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Compact and effective photon-resolved image scanning microscope

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Advanced Photonics 2024年第1期6卷 84-95页

作者： Giorgio Tortarolo Alessandro Zunino Simonluca Piazza Mattia donato Sabrina Zappone Agnieszka Pierzyńska-Mach Marco Castello giuseppe Vicidomini Istituto Italiano di Tecnologia Molecular Microscopy and SpectroscopyGenoaItaly Genoa Instruments GenoaItaly University of Genoa Dipartimento di InformaticaBioingegneriaRobotica e Ingegneria dei SistemiGenoaItaly Istituto Italiano di Tecnologia Nanoscopy and NIC@IITGenoaItaly École Polytechnique Fédérale de Lausanne Laboratory of Experimental BiophysicsLausanneSwitzerland

Fluorescence confocal laser-scanning microscopy(LSM)is one of the most popular tools for life science *** popularity is expected to grow thanks to single-photon array detectors tailored for *** detectors offer unique single-photon spatiotemporal information,opening new perspectives for gentle and quantitative superresolution ***,a flawless recording of this information poses significant challenges for the microscope data acquisition(DAQ)*** present a DAQ module based on the digital frequency domain principle,able to record essential spatial and temporal features of *** use this module to extend the capabilities of established imaging techniques based on single-photon avalanche diode(SPAD)array detectors,such as fluorescence lifetime image scanning ***,we use the module to introduce a robust multispecies approach encoding the fluorophore excitation spectra in the time ***,we combine time-resolved stimulated emission depletion microscopy with image scanning microscopy,boosting spatial *** results demonstrate how a conventional fluorescence laser scanning microscope can transform into a simple,information-rich,superresolved imaging system with the simple addition of a SPAD array detector with a tailored data acquisition *** expected a blooming of advanced single-photon imaging techniques,which effectively harness all the sample information encoded in each photon.

关键词： fluorescence lifetime image scanning microscopy digital frequency domain single photon

Fractional-order systems without equilibria:The first example of hyperchaos and its application to synchronization

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Chinese Physics B 2015年第8期24卷 224-232页

作者： donato Cafagna giuseppe Grassi Dipartimento Ingegneria Innovazione Università del Salento

A challenging topic in nonlinear dynamics concerns the study of fractional-order systems without equilibrium *** particular, no paper has been published to date regarding the presence of hyperchaos in these systems. This paper aims to bridge the gap by introducing a new example of fractional-order hyperchaotic system without equilibrium points. The conducted analysis shows that hyperchaos exists in the proposed system when its order is as low as 3.84. Moreover, an interesting application of hyperchaotic synchronization to the considered fractional-order system is provided.

关键词： fractional-order systems equilibrium points hyperchaotic systems synchronization

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Solar-Electric Boat

Journal of Transportation Technologies 2012年第2期2卷 144-149页

作者： giuseppe Schirripa Spagnolo donato Papalillo Andrea Martocchia giuseppe Makary department of Electronic Engineering University “Roma Tre” Roma Italy

The aim of this paper is the design of a Solar-Electric Boat for tourists’ transport along the coast, in the rivers, in the lakes. Our idea is to define the project guidelines for the realization of a zero impact boat. This paper illustrates the practical new technologies (naval architecture small craft design, mechanical and electrical design), rational design and engineering approach, safety and reliability methods used in solar boats. In our project, the boat is powered by lithiumion batteries that can be charged at any time by the photovoltaic generator placed on a flat top structure. The project is designed for brief trip around coast, where the public transport becomes very polluting during summer. Starting from the consideration that this boat is used during sunny weather, it is possible to know the boat’s energy demand and proceed with the design of a suitable electric boat and of the energy storage/management system. It is also proposed an innovative management of charge/discharge of the batteries. With this management, we have optimized the use and prolonged the time of life of the batteries during the navigation and the control of the real autonomy of it.

关键词： Solar-Electric Ship Electric Propulsion Photovoltaic Lithium-Ion Batteries

Consensus statement AIGO/SICCR diagnosis and treatment of chronic constipation and obstructed defecation(Part Ⅱ:Treatment)

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World Journal of Gastroenterology 2012年第14期18卷 4994-5013页

作者： Antonio Bove Massimo Bellini Edda Battaglia Renato Bocchini Dario Gambaccini Vincenzo Bove Filippo Pucciani donato Francesco Altomare giuseppe Dodi Guido Sciaudone Ezio Falletto Vittorio Piloni Gastroenterology and Endoscopy Unit Department of GastroenterologyAORN "A.Cardarelli"80131 NaplesItaly Gastrointestinal Unit Department of GastroenterologyUniversity of Pisa56100 PisaItaly Gastroenterology and Endoscopy Unit Cardinal Massaja Hospital14100 AstiItaly Gastroenterology Unit M.Bufalini Hospital47023 CesenaItaly department of Medical and Surgical Critical Care University of Florence50141 FlorenceItaly department of Emergency and Organ Transplantation University of Bari70124 BariItaly department of Oncological and Surgical Sciences University of Padua35126 PaduaItaly Division of General and Geriatric Surgery Second University of Naples80131 NaplesItaly Sixth Division of University Surgery Department of General SurgerySan Giovanni Battista Hospital10126 TurinItaly Diagnostic Imaging Centre "N.Aliotta" Villa Silvia-SenigalliaI-60100 AnconaItaly

The second part of the Consensus Statement of the Italian Association of Hospital Gastroenterologists and Italian Society of Colo-Rectal Surgery reports on the treatment of chronic constipation and obstructed *** is no evidence that increasing fluid intake and physical activity can relieve the symptoms of chronic *** with normal-transit constipation should increase their fibre intake through their diet or with commercial *** laxatives may be effective in patients who do not respond to fibre *** laxatives should be reserved for patients who do not respond to osmotic *** trials have shown that serotoninergic enterokinetic agents,such as prucalopride,and prosecretory agents,such as lubiprostone,are effective in the treatment of patients with chronic *** is sometimes *** colectomy with ileorectostomy may be considered in patients with slow-transit constipation and inertia coli who are resistant to medical therapy and who do not have defecatory disorders,generalised motility disorders or psychological *** controlled trials have established the efficacy of rehabilitative treatment in dys-synergic *** surgical procedures may be used to treat obstructed defecation in patients with acquired anatomical defects,but none is considered to be the gold *** should be reserved for selected patients with an impaired quality of *** defecation is often associated with pelvic organ *** with the placement of prostheses is replacing fascial surgery in the treatment of pelvic organ prolapse,but the efficacy and safety of such procedures have not yet been established.

关键词：外科治疗排便便秘慢性 AIGO 随机对照试验诊断手术治疗

LED Applications in Railway Signals: Wavelength and Intensity vs Temperature Variation

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Journal of Transportation Technologies 2012年第1期2卷 78-83页

作者： giuseppe Schirripa Spagnolo donato Papalillo Andrea Martocchia 不详

In recent years, light emitting diodes (LEDs) have entered the lighting market, offering consumers performance and features exceeding those of traditional lighting technologies. LEDs (light-emitting diodes) are becoming more common in safety signals for railroad, highway, automotive, and many other applications. In addition to having a longer life and greater durability than incandescent bulbs, LEDs are much more energy efficient than their incandescent counterparts. Since the heat from the junction must be dissipated into the ambient somehow, changing the ambient temperature affects the junction temperature and hence the emitted light. When the LEDs are used in the railway or traffic signals, the optical proprieties of these have to maintain more rigorous specifications. Therefore the development of signals using LED as light source, able to respect intensity specifications, is not simple. In this paper, we describe problems of the temperature dependent changes of LED intensity and color shift. Besides we will introduce an innovative technique, that we have developed, to allow the use of the LEDs in applications with rigorous specifications.

关键词： LED Temperature-dependency LED Intensity Shift Railway Signal

Pan-enteric dysmotility,impaired quality of life and alexithymia in a large group of patients meeting ROMEⅡ criteria for irritable bowel syndrome

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World Journal of Gastroenterology 2003年第10期9卷 2293-2299页

作者： Piero Portincasa Antonio Moschetta giuseppe Baldassarre donato F. Altomare giuseppe Palasciano Section of Internal Medicine Department of Internal Medicine and Public Medicine(DIMIMP)University Medical SchoolBari Division of Geriatrics Hospital"Miulli"Acquaviva delle FontiBariItaly Section of-Surgery Department of Emergency and Organ Transplant(DETO)University Medical SchoolBariItaly

AIM:Psychological factors, altered motility and sensation disorders of the intestine can be variably associated with irritable bowel syndrome (IBS). Such aspects have not been investigated simultaneously. The aim of this paper was to evaluate gastrointestinal motility and symptoms, psychological spectrum and quality of life in a large group of IBS patients in southern ***: One hundred IBS patients (F:M=73:27, age 48±2 years, mean±SE) fulfilling ROME Ⅱ criteria matched with 100 healthy subjects (F:M=70:30, 45±2 years). Dyspepsia,bowel habit, alexithymia, psycho-affective profile and quality of life were assessed using specific questionnaires. Basally and postprandially, changes in gallbladder volumes and antral areas after liquid meal and orocaecal transit time (OCTT)were measured respectively by ultrasonography and H2-breath test. Appetite, satiety, fullness, nausea, and epigastric pain/discomfort were monitored using visual-analogue ***: Compared with controls, IBS patients had increased dyspepsia (score 12.6±0.7 VS 5.1±0.2, P＜0.0001),weekly bowel movements (12.3±0.4 VS 5.5±0.2, P＜0.00001, comparable stool shape), alexithymia (score 59.11.1 VS40.5±1.0, P=0.001), poor quality of life and psychoaffective profile. IBS patients had normal gallbladderemptying, but delayed gastric emptying (T50:35.5±1.0 VS 26.1±0.6 min, P=0.00001) and OCTT (163.0±5.4 VS96.6±1.8min, P=0.00001). Fullness, nausea, and epigastric pain/discomfort were greater in IBS than in ***: ROME Ⅱ IBS patients have a pan-enteric dysmotility with frequent dyspepsia, associated with psychological morbidity and greatly impaired quality of *** presence of alexithymia, a stable trait, is a novel finding of potential interest to detect subgroups of IBS patients with different patterns recoveed after therapy.

关键词：肠易激综合征述情障碍生活质量临床研究

The HEPD particle detector of the CSES satellite mission for investigating seismo-associated perturbations of the Van Allen belts

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Science China(Technological Sciences) 2018年第5期61卷 643-652页

作者： AMBROSI Giovanni BARTOCCI Simona BASARA Laurent BATTISTON Roberto BURGER William J. CARFORA Luca CASTELLINI Guido CIPOLLONE Piero CONTI Livio CONTIN Andrea de donato Cinzia de SANTIS Cristian FOLLEGA Francesco M. GUANDALINI Cristina IONICA Maria IUPPA Roberto LAURENTI Giuliano LAZZIZZERA Ignazio LOLLI Mauro MANEA Christian MARCELLI Laura MASCIANTONIO giuseppe MERGE Matteo OSTERIA giuseppe PACINI Lorenzo PALMA Francesco PALMONARI Federico PANICO Beatrice PATRIZII Laura PERFETTO Francesco PICOZZA Piergiorgio POZZATO Michele PUEL Matteo RASHEVSKAYA Irina RICCI Ester RICCI Marco RICCIARINI Sergio Bruno SCOTTI Valentina SOTGIU Alessando SPARVOLI Roberta SPATARO Bruno VITALE Vincenzo Istituto Nazionale di Fisica Nucleare (INFN) Sezione di Perugia Perugia 06123 Italy Uninettuno University Rome 00186 Italy Istituto Nazionale di Fisica Nucleare (INFN) Sezione di Roma Tor Vergata Rome 00133 Italy Istituto Nazionale di Fisica Nucleare (INFN) TIFPA Povo (TN) 38123 Italy Dipartimento di Fisica Universita di Trento Povo (TN) 38123 Italy Dipartimento di Fisica Universita di Roma Tor Vergata Rome 00133 Italy IFAC-CNR Sesto Fiorentino (FI) 50019 Italy Dipartimento di Fisica e Astronomia Universita di Bologna Bologna 40127 Italy Istituto Nazionale di Fisica Nucleare (INFN) Sezione di Bologna Bologna 40127 Italy Istituto Nazionale di Fisica Nucleare (INFN) Sezione di Napoli Naples 80126 Italy Istituto Nazionale di Fisica Nucleare (INFN) LNE Frascati (RM) 00044 Italy Agenzia Spaziale Italiana (ASI) SSDC Rome 00133 Italy

CSES(China Seismo-Electromagnetic Satellite) is a mission developed by CNSA(Chinese National Space Administration) and ASI(Italian Space Agency), to investigate the near-Earth electromagnetic, plasma and particle environment, for studying the seismo-associated disturbances in the ionosphere-magnetosphere transition zone. The anthropogenic and electromagnetic noise,as well as the natural non-seismic electromagnetic emissions is mainly due to tropospheric activity. In particular, the mission aims to confirming the existence of possible temporal correlations between the occurrence of earthquakes for medium and strong magnitude and the observation in space of electromagnetic perturbations, plasma variations and precipitation of bursts with highenergy charged particles from the inner Van Allen belt. In this framework, the high energy particle detector(HEPD) of the CSES mission has been developed by the Italian LIMADOU Collaboration. HEPD is an advanced detector based on a tower of scintillators and a silicon tracker that provides good energy and angular resolution and a wide angular acceptance, for electrons of 3–100 Me V, protons of 30–200 Me V and light nuclei up to the oxygen. CSES satellite has been launched on February 2^(nd), 2018 from the Jiuquan Satellite Launch Center(China).

关键词：卫星发射中心粒子带子空间管理合作开发空间机构轻原子核意大利

通过家庭护士主导的健康教育减少慢性病患者就医的试点研究

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International Journal of Nursing Sciences 2021年第3期8卷 264-270,I0002页

作者： Serenella Savini Paolo Iovino Dario Monaco Roberta Marchini Tiziana Di Giovanni giuseppe donato Ausilia Pulimeno Carmela Matera giuseppe Quintavalle Carlo Turci ASL Rome 4 RomeItaly department of Biomedicine and Prevention University of Rome Tor VergataRomeItaly School of Nursing Midwifery and paramedicine Faculty of health sciencesAustralian Catholic UniversityMelbourneAustralia Presidente Ordine Professioni Infermieristiche RomeItaly

目的老年慢性病患者往往需要频繁得到医疗服务.这项研究旨在评估由家庭护士主导的结构化教育干预措施是否可以有效地减少受老年慢性病患者医疗服务使用(再入院和/或急救服务)频次.方法采用非随机前后对照研究.从意大利的2个综合性诊所... 详细信息

目的老年慢性病患者往往需要频繁得到医疗服务.这项研究旨在评估由家庭护士主导的结构化教育干预措施是否可以有效地减少受老年慢性病患者医疗服务使用(再入院和/或急救服务)频次.方法采用非随机前后对照研究.从意大利的2个综合性诊所招募了78例患者,其中70例完成了历时8个月的研究.前4个月进行了标准的居家护理,随后进行结构化教育干预,直至研究结束.基于回授法的教育干预包括每周1次60 min家庭会议,内容针对患者所患疾病及其治疗、潜在并发症、药物依从性和健康行为等方面.收集教育干预前后老年患者的医疗服务使用频率.采用McNemar检验分析教育干预前后医疗服务利用率的差异,通过Cox风险回归模型分析与医疗服务使用有关的潜在因素.结果本组(n=78)主要为女性(n=50,64.1%),平均年龄76.2(SD=4.8)岁.糖尿病是最常见的疾病(n=27,34.6%).McNemar分析结果表明,健康教育干预后患者医疗服务的使用频率明显减少(χ^(2)=28.03,P<0.001).Cox回归分析结果表明,时间和患者教育程度均与卫生服务使用概率有关,时间和患者教育程度的交互效应也与之存在关联.结论由家庭护士执业者主导的回授法教育干预措施有可能减少老年慢性病患者的医疗服务使用.

关键词：老年人慢性病设施和服务利用家庭护士家庭护理服务健康服务患者教育患者再入院

Leaf nutrient content and transcriptomic analyses of endive (Cichorium endivia) stressed by downpour-induced waterlog reveal a gene network regulating kestose and inulin contents

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Horticulture Research 2021年第1期8卷 1386-1401页

作者： Giulio Testone Anatoly Petrovich Sobolev Giovanni Mele Chiara Nicolodi Maria Gonnella giuseppe Arnesi Tiziano Biancari donato Giannino Institute for Biological Systems National Research Council(CNR)Via Salaria Km 29300-00015 MonterotondoRomeItaly Institute of Sciences of Food Production CNR.Via G.Amendola 122/O-70126BariItaly Enza Zaden Italia Strada Statale Aurelia km.96.400–01016 TarquiniaViterboItaly

Endive(Cichorium endivia L.),a vegetable consumed as fresh or packaged salads,is mostly cultivated outdoors and known to be sensitive to waterlogging in terms of yield and ***,metabolic and transcriptomic analyses were used to study variations in curly-(‘Domari’,‘Myrna’)and smooth-leafed(‘Flester’,‘Confiance’)cultivars grown in short-term waterlog due to rainfall excess before *** recording loss of head weights in all cultivars(6-35%),which was minimal in‘Flester’,NMR untargeted profiling revealed variations as influenced by genotype,environment and interactions,and included drop of total carbohydrates(6–50%)and polyols(3–37%),gain of organic acids(2–30%)and phenylpropanoids(98–560%),and cultivar-specific fluctuations of amino acids(−37 to+15%).The analysis of differentially expressed genes showed GO term enrichment consistent with waterlog stress and included the carbohydrate metabolic *** loss of sucrose,kestose and inulin recurred in all cultivars and the sucrose-inulin route was investigated by covering over 50 genes of sucrose branch and key inulin synthesis(fructosyltransferases)and catabolism(fructan exohydrolases)*** lowered expression of a sucrose gene subset together with that of SUCROSE:SUCROSE-1-FRUCTOSYLTRANSFERASE(1-SST)may have accounted for sucrose and kestose contents drop in the leaves of waterlogged *** anti-correlated modules harbouring candidate hub-genes,including 1-SST,were identified by weighted gene correlation network analysis,and proposed to control positively and negatively kestose *** silico analysis further pointed at transcription factors of GATA,DOF,WRKY types as putative regulators of 1-SST.

关键词： process. weights subset