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Cytokinome profile evaluation in patients with hepatitis C virus infection

World Journal of Gastroenterology 2014年第28期20卷 9261-9269页

作者： Francesca Capone Eliana Guerriero Giovanni Colonna Patrizia Maio Alessandra Mangia giuseppe castello Susan Costantini Centro Ricerche Oncologiche di Mercogliano IstitutoNazionale per lo Studio e la CuradeiTumori "Fondazione Giovanni Pascale"IRCCS Biochemistry Biophysics and General Pathology DepartmentSecond University of Naples Department of Infectious Diseases "San Giuseppe Moscati" Hospital Liver Unit IRCCS Casa SollievodellaSofferenza Hospital

The ‘‘omics sciences’’ (genomics, transcriptomics, proteomics) are often used to study living organisms as a whole system by evaluating the complex expression patterns of genes, miRNA, proteins, and metabolites. This study aimed, through bioinformatics and systems biology, to decipher the cytokinome profile in the evolution of inflammatory processes leading to cancer. The cytokinome was defined as the totality of cytokines and their interactions in and around biological cells. The system biology approach would provide a better understanding of the complex interaction network of cytokines, especially in cancer patients. Acquired knowledge would enable health providers with tools to evaluate disease onset through progression as well as identifying innovative therapeutic strategies. Understanding the role each cytokine plays in the metabolic network is of great importance. This paper reviews our group’s ‘‘omics’’ work. In particular, it addresses the role cytokines play in liver disease in six different scenarios. The first is the role the cytokines play in chronic inflammatory diseases and cancers. The second is the significance of the cytokinome profile. The third is the role of liver cirrhosis as an inflammatory disease. The fourth is the comparison of cytokine levels evaluated in patients with chronic hepatitis C virus (HCV) or with HCV-related cirrhosis. The fifth is the comparison of cytokine levels evaluated in patients with HCV-related cirrhosis in the presence and absence of type 2 diabetes. And lastly, we present a comparison of cytokine levels evaluated in patients with HCV-related cirrhosis in the presence and absence of hepatocellular carcinoma.

关键词： Cytokinome Hepatitis C virus Cirrhosis Type 2 diabetes Hepatocellular carcinoma Interactome Systems biology

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Compact and effective photon-resolved image scanning microscope

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Advanced Photonics 2024年第1期6卷 84-95页

作者： Giorgio Tortarolo Alessandro Zunino Simonluca Piazza Mattia Donato Sabrina Zappone Agnieszka Pierzyńska-Mach Marco castello giuseppe Vicidomini Istituto Italiano di Tecnologia Molecular Microscopy and SpectroscopyGenoaItaly Genoa Instruments GenoaItaly University of Genoa Dipartimento di InformaticaBioingegneriaRobotica e Ingegneria dei SistemiGenoaItaly Istituto Italiano di Tecnologia Nanoscopy and NIC@IITGenoaItaly École Polytechnique Fédérale de Lausanne Laboratory of Experimental BiophysicsLausanneSwitzerland

Fluorescence confocal laser-scanning microscopy(LSM)is one of the most popular tools for life science *** popularity is expected to grow thanks to single-photon array detectors tailored for *** detectors offer unique single-photon spatiotemporal information,opening new perspectives for gentle and quantitative superresolution ***,a flawless recording of this information poses significant challenges for the microscope data acquisition(DAQ)*** present a DAQ module based on the digital frequency domain principle,able to record essential spatial and temporal features of *** use this module to extend the capabilities of established imaging techniques based on single-photon avalanche diode(SPAD)array detectors,such as fluorescence lifetime image scanning ***,we use the module to introduce a robust multispecies approach encoding the fluorophore excitation spectra in the time ***,we combine time-resolved stimulated emission depletion microscopy with image scanning microscopy,boosting spatial *** results demonstrate how a conventional fluorescence laser scanning microscope can transform into a simple,information-rich,superresolved imaging system with the simple addition of a SPAD array detector with a tailored data acquisition *** expected a blooming of advanced single-photon imaging techniques,which effectively harness all the sample information encoded in each photon.

关键词： fluorescence lifetime image scanning microscopy digital frequency domain single photon

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Impaired diastolic function in nave untreated human immunodeficiency virus infected patients

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World Journal of Cardiology 2010年第4期2卷 98-103页

作者： Ugo Oliviero Giovanni Bonadies Giorgio Bosso Maria Foggia Valentina Apuzzi Mariarosaria Cotugno Antonio Valvano Enrico Leonardi Guglielmo Borgia giuseppe castello Raffaele Napoli Luigi Saccà Department of Inter-nal Medicine University Federico Ⅱ80131 NaplesItaly Department of Infectious Diseases University Federico Ⅱ80131 NaplesItaly National Cancer Institute G.Pascale Foundation80131 NaplesItaly

AIM:To evaluate cardiac function and structure in un-treated human immunodeficiency virus(HIV) patients without clinical evidence of cardiovascular ***:Fifty-three nave untreated HIV-infected patients and 56 healthy control subjects underwent clinical assessment,electrocardiography(ECG) and echocardiography,including tissue doppler ***,a set of laboratory parameters was obtained from all subjects,including HIV-RNA plasma levels,CD4 cell counts and tumor necrosis factor-α ***:The two groups showed normal ECG traces and no differences regarding systolic morphologic *** contrast,a higher prevalence of left ventricular diastolic dysfunction(abnormal relaxation or pseudonormal filling pattern) was found in the HIV patients(36% vs 9% in patients and controls,respec-tively,P <0.001).CONCLUSION:Subclinical cardiac abnormalities ap-pear in an early stage of the HIV infection,indepen-dent of antiretroviral *** data suggest that HIV per se plays a role in the genesis of diastolic dys-function.

关键词： Diastolic function Human immunodeficiency virus Naive subjects Cardiovascular risk factors

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Confocal-based fluorescence fluctuation spectroscopy with a SPAD array detector

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Light(Science & Applications) 2021年第2期10卷 289-300页

作者： Eli Slenders Marco castello Mauro Buttafava Federica Villa Alberto Tosi Luca Lanzanó Sami Valtteri Koho giuseppe Vicidomini Molecular Microscopy and Spectroscopy Istituto Italiano di TecnologiaGenoaItaly Dipartimento di Eiettronica Informazione e BioingegneriaPolitecmco di MilanoMilanItaly Nanoscopy and NIC@IIT lstituto Italiano di TecnologiaGenoaItaly Dipartimento di Fisica e Astronomia Universita di CataniaCataniaItaly

The combination of confocal laser-scanning microscopy(CLSM)and fluorescence fluctuation spectroscopy(FFS)is a powerful tool in studying fast,sub-resolution biomolecular processes in living cells.A detector array can further enhance CLSM-based FFS techniques,as it allows the simultaneous acquisition of several samples-essentially images-of the CLSM detection ***,the detector arrays that have previously been proposed for this purpose require tedious data corrections and preclude the combination of FFS with single-photon techniques,such as fluorescence lifetime ***,we solve these limitations by integrating a novel singie-photon-avalanche-diode(SPAD)array detector in a CLSM *** validate this new implementation on a series of FFS analyses:spot-variation fluorescence correlation spectroscopy,pair-correlation function analysis,and image-derived mean squared displacement *** predict that the unique combination of spatial and temporal information provided by our detector will make the proposed architecture the method of choice for CLSM-based FFS.

关键词： spectroscopy correction integrating

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Early onset versus late onset in Alzheimer’s disease: What is the reliable cut-off?

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Advances in Alzheimer's Disease 2013年第1期2卷 40-47页

作者： Gianfranco Spalletta Vincenzo De Luca Alessandro Padovani Luca Rozzini Roberta Perri Amalia Bruni Vincenzo Canonico Alberto Trequattrini giuseppe Bellelli Carla Pettenati Floriana Pazzelli Carlo Caltagirone Maria Donata Orfei Azienda Sanitaria Locale Città di castello Perugia Italy Centro Regionale Alzheimer Azienda Ospedaliera Guido Salvini Passirana Italy Centro Regionale Di Neurogenetica Azienda Sanitaria Provinciale Catanzaro Lamezia Terme Italy Department of Clinical and Behavioral Neurology IRCCS Santa Lucia Foundation Rome Italy Department of Geriatrics University of Naples Naples Italy Department of Neurology University of Brescia Brescia Italy Department of Neuroscience Tor Vergata University of Rome Rome Italy Department of Prevention and Clinical Medicine University of Milano-Bicocca Monza Italy Department of Psychiatry University of Toronto Toronto Canada NESMOS Department Sapienza University of Rome Rome Italy

Objective: As the literature on conventional criteria for discriminating early-onset (EO) from late-onset (LO) Alzheimer’s disease (AD) is sparse and controversial, the aim of this study was to establish a precise age at onset (AAO) criterion, by using a specific statistical procedure, and to describe the clinical characteristics of the two sub-groups. Methods: Admixture analysis was performed to establish the AAO cut-off in a multi-center study including 2000 AD patients consecutively recruited in eight Italian Memory Clinics. None of the patients were taking acetylcholinesterase inhibitors, antipsychoticor anti-depressant drugs. At the first diagnosticvisit, they were administered the Mini Mental StateExamination, the Basic and Instrumental Activities of Daily Living and the Neuropsychiatric Inventorytoassess clinical phenomenology. Results: Using a specific statistical procedure, we established that AAO that discriminated EO-from LO-AD was 66. Compared with the LO-AD group, the EO-AD group showed longer duration of illness and a higher educational level as well as less severe functional impairment and delusions. Conclusions: Differences in sociodemographic and clinical characteristics, such as duration of illness, education and delusion severity, suggested the involvement of different pathogenic processes. Additional studies are needed to further investigate the mechanisms underlying the disorder in the two sub-groups of AD patients.

关键词： Alzheimer’s Disease Onset Neuropsychiatric Symptoms Admixture Analysis Pathogenesis Cognitive Reserve

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