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Exosomes of adult human fibroblasts cultured on 3D silk fibroin nonwovens intensely stimulate neoangiogenesis

Burns & Trauma 2021年第1期9卷 488-506页

作者： Peng Hu Anna Chiarini Jun Wu giuliano Freddi Kaiyu Nie Ubaldo Armato Ilaria Dal Prà Human Histology&Embryology Section Department of SurgeryDentistryPaediatrics&GynaecologyUniversity of Verona Medical SchoolStrada Le Grazie 8I-37134VeronaVenetiaItaly Department of Burns&Plastic Surgery The Affiliated Hospital of ZunYi Medical University149 Dalian RoadZunYi City563003 Guizhou ProvinceChina Department of Burns and Plastic Surgery Second People’s HospitalUniversity of Shenzhen3002 Sungang West RoadFutian DistrictShenzhen518000Guangdong ProvinceChina Silk Biomaterials S.r.l. Via Cavour 2I-22074LomazzoLombardyItaly

Background:Bombyx mori silk fibroin is a biomacromolecule that allows the assembly of scaffolds for tissue engineering and regeneration purposes due to its cellular adhesiveness,high biocompatibility and low *** work showed that two types of 3D silk fibroin nonwovens(3D-SFnws)implanted into mouse subcutaneous tissue were promptly vascularized via undefined molecular *** present study used nontumorigenic adult human dermal fibroblasts(HDFs)adhering to a third type of 3D-SFnws to assess whether HDFs release exosomes whose contents promote ***:Electron microscopy imaging and physical tests defined the features of the novel carded/hydroentangled *** were cultured on 3D-SFnws and polystyrene plates in an exosome-depleted *** amounts and D-glucose consumption revealed the growth and metabolic activities of HDFs on ***9-expressing total exosome fractions were from conditioned media of 3D-SFnws and 2D polystyrene plates HDF *** growth factors(AGFs)in equal amounts of the two groups of exosomal proteins were analysed via doubleantibody arrays.A tube formation assay using human dermal microvascular endothelial cells(HDMVECs)was used to evaluate the exosomes’angiogenic ***:The novel features of the 3D-SFnws met the biomechanical requirements typical of human soft *** experimental day 15,3D-SFnws-adhering HDFs had increased 4.5-fold in numbers and metabolized 5.4-fold more D-glucose than at day 3 in *** to polystyrene-stuck HDFs,exosomes from 3D-SFnws-adhering HDFs carried significantly higher amounts of AGFs,such as interleukin(IL)-1α,IL-4 and IL-8;angiopoietin-1 and angiopoietin-2;angiopoietin-1 receptor(or Tie-2);growth-regulated oncogene(GRO)-α,GRO-βand GRO-γ;matrix metalloproteinase-1;tissue inhibitor metalloproteinase-1;and urokinase-type plasminogen activator surface receptor,but lesser amounts of anti-angiogenic tissue inhibitor metalloproteinase-2 and

关键词： Silk fibroin Nonwovens Dermis Fibroblast Human endothelial cell Exosome Cytokine Chemokine Angiogenesis Regeneration Tissue engineering

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Sensor with Intact or Modified Yeast Cells as Rapid Device for Toxicological Test of Chemicals

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Journal of Agricultural Chemistry and Environment 2014年第2期3卷 35-40页

作者： Roberto dragone Chiara Frazzoli Gerardo Grasso Gloria Rossi Department of Agricultural Environmental and Food Sciences University of Molise Campobasso Italy Department of Veterinary Public Health and Food Safety Istituto Superiore di Sanità Rome Italy Institute of Nanostructured Materials Consiglio Nazionale delle Ricerche Rome Italy

Aerobic catabolism of S. cerevisiae (cell respiration) is a rapid, cost-effective, and reproducible toxicological endpoint of the whole cells biosensor. To increase the signal intensity, a protocol for the immobilization and modification of the yeast cells is described. In particular, the enzymatic treatment of the immobilized yeast cells allows removing the cell wall and obtaining structurally modified cells namely spheroplasts. Both immobilization and exposure of sensitive cells like spheroplasts confirmed to improve the method’s sensitivity vs. the chemicals. The present paper reports the test of different chemicals (including Mercury and wood preservative like Tanalith) present in consumer products, performed both by sensor with intact and modified whole cells.

关键词： Screening Methods Spheroplast Whole Cell Tanalith Mercury

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Endoscopic management of intragastric penetrated adjustable gastric band for morbid obesity

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World Journal of Gastroenterology 2006年第25期12卷 4098-4100页

作者： Giovanni D De Palma Antonio Formato Vincenzo Pilone Maria Rega Maria Elena giuliano Immacolata Simeoli Pietro Forestieri Centro per l'Innovazione Tecnologica in Chirurgia (ITC)-Dipartimento di Chirurgia Generale Oncologica e Tecnologie Avanzate.Università degli Studi di Napoli Federico Ⅱ Facoltà di Medicina e Chirurgia NapoliItaly

This report describes a case of successful endoscopic management of intragastric penetrated adjustable gastric band in a patient with morbid obesity. The favorable course of the case described here demonstrates that adjustable gastric bands in the process of migration need not be removed surgically in patients who are asymptomatic.

关键词： Morbid obesity Adjustable gastric band Complications Endoscopy Endoscopic therapy

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Climatic and anthropogenic factors explain the variability of Fagus sylvatica treeline elevation in fifteen mountain groups across the Apennines

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Forest Ecosystems 2020年第1期7卷 51-63页

作者： giuliano Bonanomi Maurizio Zotti Valentina Mogavero Gaspare Cesarano Luigi Saulino Angelo Rita Giulio Tesei Marina Allegrezza Antonio Saracino Emilia Allevato Dipartimento di Agraria Universitàdi Napoli Federico Ⅱvia Università10080055 PorticiNAItaly Scuola di Scienze Agrarie ForestaliAlimentari e AmbientaliUniversitàdella BasilicataViale dell’Ateneo Lucano 1085100 PotenzaItaly Dipartimento di Scienze Agrarie Alimentari e AmbientaliUniversitàPolitecnica delle Marchevia Brecce Bianche60100 AnconaItaly.

Background:Fagus sylvatica forms the treeline across the Apennines mountain range,with an average elevation of 1589 *** studies evidenced that the current position of the treeline in the Apennines is heavily depressed as a result of a complex interaction between climatic factors and the past human *** this study we correlated treeline elevation in the fifteen major mountain groups in the Apennines with selected climatic,geomorphological,and human disturbance variables in order to investigate in detail the site-specific features affecting the current treeline ***:Treeline elevation was lowest in the North Italy(Apuan Alps),while the highest treeline was found in Central Italy(Simbruini).An absolute maximum treeline elevation of *** exceeding *** found on 13 mountain peaks in Central and Southern ***,treeline elevation was largely lower on warmer south-facing slopes compared to northern slopes,with values several hundred meters lower in the Gran Sasso and *** the causes of this pattern are still unknown,we argue that treeline elevation on southfacing slopes may be limited by the combination of climatic constraints(*** drought)and human *** of a pervasive anthropogenic effect depressing treeline elevation was found in the North(Apuan Alps)Central(Gran Sasso,Velino-Sirente,Sibillini)and Southern part of Apennines(Pollino).By contrast,treeline elevation of the Laga,Simbruini,and Orsomarso mountain groups appears less affected by past anthropogenic ***,we recorded in the several mountain groups(***,Marsicani and Pollino)the coexistence of very depressed treelines just a few kilometers away from much higher treelines,among the highest ever recorded for ***:Finally,we argue that *** treeline across the Apennines is locally shaped both by the interaction of low temperatures experienced by the species in its earli

关键词： Anthropogenic impact Latitudinal gradient Mean annual temperature Population density Summer drought Winter temperature

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Intraoperative thromboelastography as a tool to predict postoperative thrombosis during liver transplantation

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World Journal of Transplantation 2020年第11期10卷 345-355页

作者： Lesley De Pietri Roberto Montalti giuliano Bolondi Valentina Serra Fabrizio Di Benedetto Department of General Surgery Division of Anaesthesiology and Intensive Care UnitNuovo Ospedale Civile di SassuoloSassuolo 41049ModenaItaly Department of Public Health Hepato-Pancreato-Biliary Surgery SectionFederico II University of NaplesNapoli 80138Italy Surgery and Trauma Department Intensive Care UnitOspedale Bufalini CesenaCesena 47521Italy Surgery Department Hepato-Pancreato-Biliary SurgerySurgical Oncology and Liver Transplantation UnitAzienda Ospedaliero Universitaria di ModenaUniversity of Modena and Reggio EmiliaModena 41125Italy

BACKGROUND Thromboembolic complications are relatively common causes of increased morbidity and mortality in the perioperative period in liver transplant *** postoperative portal vein thrombosis(PVT,incidence 2%-2.6%)and early hepatic artery thrombosis(HAT,incidence 3%-5%)have a poor prognosis in transplant patients,having impacts on graft and patient *** the present study,we attempted to identify the predictive factors of these complications for early detection and therefore monitor more closely the patients most at risk of thrombotic *** To investigate whether intraoperative thromboelastography(TEG)is useful in detecting the risk of early postoperative HAT and PVT in patients undergoing liver transplantation(LT).METHODS We retrospectively collected thromboelastographic traces,in addition to known risk factors(cold ischemic time,intraoperative requirement for red blood cells and fresh-frozen plasma transfusion,prolonged operating time),in 27 patients,selected among 530 patients(≥18 years old),who underwent their first LT from January 2002 to January 2015 at the Liver University Transplant Center and developed an early PVT or HAT(case group).Analyses of the TEG traces were performed before anesthesia and 120 min after *** retrospectively compared these patients with the same number of nonconsecutive control patients who underwent LT in the same study period without developing these complications(1:1 match)(control group).The chosen matching parameters were:Patient graft and donor characteristics[age,sex,body mass index(BMI)],indication for transplantation,procedure details,United Network for Organ Sharing classification,BMI,warm ischemia time(WIT),cold ischemia time(CIT),the volume of blood products transfused,and conventional laboratory coagulation *** distributed continuous data are reported as the mean±SD and compared using one-way Analysis of Variance(ANOVA).Nonnormally distributed continuous data are rep

关键词： Thromboelastography Hepatic artery thrombosis Portal vein thrombosis Liver transplantation Risk factors Cirrhosis

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Differential gene expression of chemokines in KRAS and BRAF mutated colorectal cell lines:Role of cytokines

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World Journal of Gastroenterology 2014年第11期20卷 2979-2994页

作者： Sajjad Khan Silke Cameron Martina Blaschke Federico Moriconi Naila Naz Ahmad Amanzada giuliano Ramadori Ihtzaz Ahmed Malik Clinic of Gastroenterology and Endocrinology University Medical CenterRobert-Koch-Str. 4037075 GoettingenGermany

AIM: To study KRAS/BRAF mutations in colorectal-cancer (CRC) that influences the efficacy of treatment. To develop strategies for overcoming combination of treatment.

关键词： Colorectal-cancer, KRAS, BRAF, CXCL1 (GROα ), CXCL10 (IP-10), CXCL8 (IL-8), Tumor necrosis factor-α , Interleukin-1β , Interferon-γ , siRNA

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Diet high in fructose leads to an overexpression of lipocalin-2 in rat fatty liver

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World Journal of Gastroenterology 2014年第7期20卷 1807-1821页

作者： Salamah Mohammad Alwahsh Min Xu Hatice Ali Seyhan Shakil Ahmad Sabine Mihm giuliano Ramadori Frank Christian Schultze Department of Gastroenterology and Endocrinology University Medical Center Goettingen37075 GoettingenGermany Department of General Visceral and Pediatric SurgeryUniversity Medical Center Goettingen37075 GoettingenGermany

AIM: To explore lipocalin-2 (LCN-2) expression and its possible role and mechanism(s) of production in rat models of diet-inducible fatty liver.

关键词： Non-alcoholic fatty liver disease Inflammation Endotoxins Lipopolysaccharide Oxidative stress Mitochondrial dysfunction Metabolic syndrome

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Extrahepatic portal vein thrombosis in children and adolescents:Influence of genetic thrombophilic disorders

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World Journal of Gastroenterology 2010年第48期16卷 6123-6127页

作者： Andrea Pietrobattista Matteo Luciani Juan G Abraldes Manila Candusso Simona Pancotti Massimo Soldati Lidia Monti giuliano Torre Valerio Nobili Liver Unit Research InstituteBambino Gesù Children's Hospital Department of Hematology Bambino Gesù Children's Hospital Hepatic Hemodynamic Laboratory Liver UnitHospital ClínicIDIBAPS and CIBERhedUniversity of Barcelona Department of Laboratory Pediatrics Bambino Gesù Children's Hospital Radiology Department Bambino Gesù Children's Hospital

AIM:To explore the prevalence of local and genetic thrombophilic disorders as risk factors for portal vein thrombosis (PVT) in our series,the largest ever published in pediatric literature. METHODS:We conducted a case-control study enrolling 31 children with PVT and 26 age-matched controls. All were screened for thrombophilia,including genetic disorders,protein C,protein S and homocysteine deficiencies. All coagulation parameters were studied at least 3 mo after the diagnosis of portal vein ***:In our study we showed that most pediatric patients with PVT have local prothrombotic risk factors,which are probably the most important factors leading to PVT. However,there is a clear association between the presence of prothrombotic disorders and PVT,suggesting that these increase the risk of thrombosis in patients with local factors such as perinatal umbilical vein catheterization or sepsis. CONCLUSION:Patients with PVT should be screened for inherited prothrombotic disorders regardless of a history of an obvious local risk factor.

关键词： Portal vein thrombosis Children Thrombophilic disorders Protein C Protein S

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Uganda

Uganda

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作者： edited by Jorg Wiegratz giuliano Martiniello Elisa Greco.

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核医学在甲状腺良性疾病临床处治中的应用(第1部分):甲状腺功能亢进症

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中华核医学与分子影像杂志 2022年第10期42卷 633-640页

作者： giuliano Mariani Massimo Tonacchera Mariano Grosso Francesca Orsolini Paolo Vitti H.William Strauss 肖柳(译) 杨柳(译) 李林(审校) Department of Translational Research and Advanced Technologies in Medicine and Surgery Regional Center of Nuclear MedicineUniversity of PisaPisaItaly Endocrinology Unit Department of Clinical and Experimental MedicineUniversity of PisaPisaItaly Regional Center of Nuclear Medicine University Hospital of PisaPisaItaly Molecular Imaging and Therapy Service Memorial Sloan Kettering Cancer CenterNew YorkNew York 四川大学华西医院核医学科成都610041

甲状腺良性疾病,尤其是甲状腺功能亢进症(简称甲亢)和甲状腺功能减退症(简称甲减),是最常见的内分泌疾病。甲亢常见的病因是自身免疫性甲亢(格雷夫斯病,即GD)、毒性多结节性甲状腺肿(TMNG)、毒性甲状腺腺瘤(TAs);其他少见病因包括破坏... 详细信息

甲状腺良性疾病,尤其是甲状腺功能亢进症(简称甲亢)和甲状腺功能减退症(简称甲减),是最常见的内分泌疾病。甲亢常见的病因是自身免疫性甲亢(格雷夫斯病,即GD)、毒性多结节性甲状腺肿(TMNG)、毒性甲状腺腺瘤(TAs);其他少见病因包括破坏性甲状腺炎(胺碘酮导致的甲状腺功能异常)和人为甲亢。GD主要由促甲状腺激素受体抗体(TRAb)引起;TMNG和TAs由体细胞功能激活获得突变引起。甲亢患者典型的实验室表现为促甲状腺激素(TSH)降低,游离三碘甲状腺原氨酸(FT3)和游离甲状腺素(FT4)升高,以及GD患者TSH受体自身抗体升高。超声检查主要用于明确甲状腺大小、血供以及甲状腺结节的位置、大小、数目及影像特征。甲状腺超声和实验室检查是明确甲亢病因的一线诊断方法。甲状腺显像(^(131)I或99TcmO4-甲状腺显像)可用于鉴别不同类型的甲亢,为^(131)I治疗提供策略指导。甲状腺显像可帮助鉴别GD、TMNG、TAs和破坏性甲状腺炎。^(99)Tc^(m)O_(4)^(-)可鉴别胺碘酮导致的1型和2型甲亢。测定甲状腺摄碘率(RAIU)有助于明确甲亢^(131)I治疗的放射性活度。治疗甲亢的方案主要包括抗甲状腺药物(ATD)治疗、手术或^(131)I治疗。当患者ATD治疗失败、或有手术治疗禁忌或拒绝手术,可以考虑^(131)I治疗。对于TMNG或TAs患者,^(131)I治疗目的在于恢复正常甲状腺功能。对于GD患者,^(131)I治疗的目的在于诱发甲减,这种状态易通过口服甲状腺激素替代疗法治疗。可根据甲状腺大小和RAIU的计算剂量法指导^(131)I治疗的放射性活度。^(131)I治疗的早期毒性及不良反应(甲状腺床区轻微疼痛)可通过非甾体抗炎药来处理;晚期毒性及不良反应主要是甲减和辐射诱导的恶性肿瘤(发生率极低)。

关键词：甲状腺功能亢进症毒性多结节性甲状腺肿毒性甲状腺腺瘤破坏性甲状腺炎胺碘酮导致的甲状腺功能异常自身免疫性

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