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Purification and Characterization of Glucose-6-Phosphate Dehydrogenase from Pigeon Pea (Cajanus cajan) Seeds

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Advances in Enzyme Research 2014年第4期2卷 134-149页

作者： Siddhartha Singh Pramod kumar srivastava Department of Biochemistry Faculty of Science Banaras Hindu University Varanasi India Department of Basic Science & Humanities College of Horticulture and Forestry Central Agricultural University Pasighat India

Glucose-6-phosphate dehydrogenase has been purified from pigeon pea (Cajanus cajan) seeds and subjected to characterization. The enzyme was purified 123.69 fold with a yield of 21.37% by ammonium sulphate fractionation, PEG-4000 precipitation, CM cellulose column chromatography and DEAE cellulose column chromatography. The catalytically active enzyme is a dimer of 113 KDa with a subunit molecular weight of 55 KDa. Thermal inactivation of enzyme follows first order kinetics at 30°C and 40°C with half life of 6 and 1.5 min respectively. Km value for glucose-6-phosphate and NADP+ was found to be 2.68 mM and 0.75 mM respectively whereas Vmax value was found to be 0.11 U/mL and 0.13 U/mL respectively. The enzyme shows more affinity towards NADP+ than glucose-6-phosphate. The pKa value was found to be 10.41 indicating that the amino acid residue at active site might be lysine. The enzyme exhibited maximum catalytic activity at pH 8.2. The enzyme was found to be highly thermosensitive with gradual loss of activity above 30°C temperature.

关键词： Purification Characterization Enzyme Glucose-6-Phosphate Dehydrogenase Pigeon Pea

Control Framework for Secure Cloud Computing

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Journal of Information Security 2015年第1期6卷 12-23页

作者： Harshit srivastava Sathish Alampalayam kumar Information Technology Maharaja Agrasen Institute of Technology New Delhi India Computer Science and Information Systems Coastal Carolina University Conway USA

Cloud computing is touted as the next big thing in the Information Technology (IT) industry, which is going to impact the businesses of any size and yet the security issue continues to pose a big threat on it. The security and privacy issues persisting in cloud computing have proved to be an obstacle for its widespread adoption. In this paper, we look at these issues from a business perspective and how they are damaging the reputation of big companies. There is a literature review on the existing issues in cloud computing and how they are being tackled by the Cloud Service Providers (CSP). We propose a governing body framework which aims at solving these issues by establishing relationship amongst the CSPs in which the data about possible threats can be generated based on the previous attacks on other CSPs. The Governing Body will be responsible for Data Center control, Policy control, legal control, user awareness, performance evaluation, solution architecture and providing motivation for the entities involved.

关键词： Cloud Computing Security Privacy Organization Control Governance Framework Cloud Provider

Next-Generation Intelligent MXene-Based Electrochemical Aptasensors for Point-of-Care Cancer Diagnostics

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Nano-Micro Letters 2022年第6期14卷 248-281页

作者： Arpana Parihar Ayushi Singhal Neeraj kumar Raju Khan MohdAkram Khan Avanish Ksrivastava Industrial Waste Utilization Nano and BiomaterialsCSIR-Advanced Materials and Processes Research Institute(AMPRI)Hoshangabad RoadBhopal 462026MPIndia Academy of Scientific and Innovative Research(AcSIR) Ghaziabad 201002India

Delayed diagnosis of cancer using conventional diagnostic modalities needs to be addressed to reduce the mortality rate of ***,2 D nanomaterial-enabled advanced biosensors have shown potential towards the early diagnosis of *** high surface area,surface functional groups availability,and excellent electrical conductivity of MXene make it the 2 D material of choice for the fabrication of advanced electrochemical biosensors for disease ***-enabled electrochemical aptasensors have shown great promise for the detection of cancer biomarkers with a femtomolar limit of ***,the stability,ease of synthesis,good reproducibility,and high specificity offered by MXene-enabled aptasensors hold promise to be the mainstream diagnostic *** this review,the design and fabrication of MXene-based electrochemical aptasensors for the detection of cancer biomarkers have been ***,various synthetic processes and useful properties of MXenes which can be tuned and optimized easily and efficiently to fabricate sensitive biosensors have been ***,futuristic sensing applications along with challenges will be deliberated herein.

关键词： MXene Electrochemical devices POCT Aptamer Cancer diagnostics

Effect of micro-environmental factors on natural regeneration of Sal (Shorea robusta)

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Journal of Forestry Research 2011年第4期22卷 543-550页

作者： J. V. Tyagi Rakesh kumar S. L. srivastava R. D. Singh National Institute of Hydrology Jalvigyan Bhawan Roorkee - 247 667Uttarakhand India

factors viz., soil moisture and light in- tensity are important factors that affect natural regeneration in forests. These factors vary spatially depending on the overhead canopy density of the forest. The present study focused on studying the effect of variation of soil moisture and light intensity on natural regeneration of sal species （Shorea robusta） under different micro-environments due to overhead canopy of varying forest density. Experimental plots of 40m× 40m size were laid under different overhead canopy densities in a small sal forested watershed in the foot hills of Himalayas in Nainital District of Uttarakhand State, India. The plots were monitored on a long term basis for soil moisture at multi depths, light intensity and natural regeneration of sal. The results of the study revealed that the natural regeneration was highest under C1 （up to-0.30） canopy followed by C2 （0.30-0.50）, and C3 （0.50-0.70） canopies. The C3 canopy showed the dying back of sal shoots over 4 years of study. The highest R2 value of linear regression between incremental score of plot regeneration and average soil moisture content was obtained as 0.156 for average soil moisture content during months at 100 cm depth. The R2 value between incremental score of plot regeneration and annual average light intensity was obtained as 0.688 which indicated that the regeneration is largely dependent on the light intensity conditions during the year. The multiple linear regression analysis between the incremental score of regeneration and the average light intensity and average soil moisture content revealed that that about 80% of variation in regeneration is explained by both the factors.

关键词： Natural regeneration sal canopy density soil moisture,light intensity

Artificial Neural Network Modeling for Sorption of Cadmium from Aqueous System by Shelled Moringa Oleifera Seed Powder as an Agricultural Waste

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Journal of Water Resource and Protection 2010年第4期2卷 339-344页

作者： Abhishek Kardam kumar Rohit Raj Jyoti kumar Arora Man Mohan srivastava Shalini srivastava 不详

A two-layer Artificial Neural Network (ANN) model was developed to predict the removal efficiency of Cd(II) ions from aqueous solution using shelled Moringa Oleifera seed (SMOS) powder. Batch experiments re-sulted into standardization of optimum conditions: biomass dosage (4.0 g), Cd(II) concentration (25 mg/L) volume (200 mL) at pH 6.5. A time of forty minutes was found sufficient to achieve the equilibrium. The ANN model was designed to predict sorption efficiency of SMOS for target metal ion by combining back propagation (BP) with principle component analysis. A sigmoid axon was used as transfer function for input and output layer. The Levenberg-Marquardt algorithm (LMA) was applied, giving a minimum mean squared error (MSE) for training and cross validation at the ninth place of decimal.

关键词： Artificial Neural Network Biosorption SMOS Cd(II) Removal

Comparative analysis of real-world data of frequent treatment sequences in metastatic prostate cancer

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Current Urology 2024年第2期18卷 104-109页

作者： Jiten Jaipuria Ishleen Kaur Mohammad Najmud Doja Tanvir Ahmad Amitabh Singh Sudhir kumar Rawal Vineet Talwar girish Sharma Uro-oncology division Rajiv Gandhi Cancer Institute and Research CentreNew DelhiIndia Amity Centre for Cancer Epidemiology and Cancer Research Amity Institute of BiotechnologyAmity UniversityNoidaIndia School of Engineering and Technology Vivekananda Institute of Professional Studies–Technical CampusNew DelhiIndia Department of Computer Engineering Jamia Millia IslamiaNew DelhiIndia Indian Institute of Information Technology SonepatIndia Department of MedicalOncology Rajiv Gandhi Cancer Institute and Research CentreNew DelhiIndia

Background:The incidence of prostate cancer is increasing worldwide.A significant proportion of patients developmetastatic disease and are initially prescribed androgen deprivation therapy(ADT).However,subsequent sequences of treatments in real-world settings that may improve overall survival remain an area of active *** and methods:Data were collected from 384 patients presenting with de novo metastatic prostate cancer from 2011 to 2015 at a tertiary cancer *** were categorized into surviving(n=232)and deceased(n=152)groups at the end of 3 *** sequence pattern mining techniques(Generalized Sequential Pattern Mining and Sequential Pattern Discovery using Equivalence Classes)were applied to determine the exact order of the most frequent sets of treatments in each ***:Degarelix,as the initial form of ADT,was uniquely in the surviving *** sequence of ADT followed by abiraterone and docetaxel was uniquely associated with a higher 3-year overall *** followed by fosfestrol was found to have a unique niche among surviving patients with a long duration of response to the initial *** who received chemotherapy followed by radiotherapy and those who received radiotherapy followed by chemotherapy were found more frequently in the deceased ***:We identified unique treatment sequences among surviving and deceased patients at the end of 3 *** should be the preferred formof *** who received ADT followed by abiraterone and chemotherapy showed better *** requiring palliative radiation and chemotherapy in any sequence were significantlymore frequent in the deceased group,identifying the need to offer such patients the most efficacious agents and to target them in clinical trial design.

关键词： Hormone therapy Metastatic prostate cancer Sequence mining Survival

Neural Network Modeling for Ni(II) Removal from Aqueous System Using Shelled Moringa Oleifera Seed Powder as an Agricultural Waste

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Journal of Water Resource and Protection 2010年第4期2卷 331-338页

作者： kumar Rohit Raj Abhishek Kardam Jyoti kumar Arora Man Mohan srivastava Shalini srivastava 不详

A single-layer Artificial Neural Network (ANN) model was developed to predict the removal efficiency of Ni(II) ions from aqueous solution using shelled Moringa Oleifera seed (SMOS) powder. Batch experiments resulted into standardization of optimum conditions: biomass dosage (4.0 g), Ni(II) concentration (25 mg/L) volume (200 mL) at pH 6.5. A time of forty minutes was found sufficient to achieve the equilibrium. The ANN model was designed to predict sorption efficiency of SMOS for target metal ion by combining back propagation (BP) with principle component analysis. A sigmoid axon was used as transfer function for input and output layers. The Levenberg–Marquardt Algorithm (LMA) was applied, giving a minimum mean squared error (MSE) for training and cross validation at the ninth place of decimal.

关键词： Artificial Neural Networks Biosorption Moringa Oleifera Ni(II) Removal

Avoiding second donor site and the second set of neck vessels in a case of absent peroneal skin perforators

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Plastic and Aesthetic Research 2022年第1期9卷 661-666页

作者： Aditya Narayan Choudhary Rajan Arora Kripa Shanker Mishra Ravi Kiran Naalla Saket srivastava Ajay kumar Dewan Department of Reconstructive and Microvascular Surgery Rajiv Gandhi Cancer Institute and Research CentreRohiniNew Delhi 110085India Department of Surgical Oncology Rajiv Gandhi Cancer Institute and Research CentreRohiniNew Delhi 110085India

This report describes the procedure of a case in which the skin paddle of the free fibula flap derived its supply solely from a soleal musculocutaneous perforator originating from the posterior tibial *** contrast,the osteo-muscular component was supplied by the peroneal vascular *** harvested the skin paddle with its vascular supply from the posterior tibial artery separately,and the osteo-muscular fibula was harvested with its supply from peroneal *** this way,we avoided violation of the second donor site for the skin *** addition,we used the distal end of peroneal vessels to salvage our skin paddle instead of sacrificing another set of neck vessels for *** technique may also be utilised in cases where the neck vessels may not be available due to previous surgeries,radiation therapy,or decision by the surgery team to not sacrifice two sets of neck vessels for anastomosis.

关键词： Free fibula flap peroneal vessels posterior tibial vessels mandible reconstruction flaps in series

Bacterial biofilms in human gastrointestinal tract: An intricate balance between health and inflammatory bowel diseases

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World Journal of Pharmacology 2019年第3期8卷 26-40页

作者： Niharika Chandra Ankita srivastava Sunil kumar Faculty of Biotechnology Institute of Bio-Sciences and Technology Shri Ramswaroop Memorial University Uttar Pradesh 225003 India Faculty of Bio-Sciences Institute of Bio-Sciences and Technology Shri Ramswaroop Memorial University Uttar Pradesh 225003 India

Inflammatory bowel disease (IBD) has been a worldwide health problem. It is characterized by severe intestinal inflammation due to immune responses against the gut microbes in genetically susceptible individuals. The understanding of gut microbiota for its composition and complex interaction in normal and diseased conditions has been assisted by the use of molecular, metagenomics and meta transcriptomics studies. The alteration of intestinal microbiota is the key determinant in the degree of inflammation caused and the prolonged course of disease. The relationship between luminal gut bacteria and innate immunity is also of prime significance. Such developments have further led to the search of specific (including bacteria and fungi) as a causative agent of IBD. Although detailed research has been done for the role of gut microbiota in IBD, molecular mechanisms and related gene expression are still not well understood in this disease, which hampers the generation of effective therapeutic agents for IBD. This paper assessed various factors contributing to IBD, genetic dysbiosis and pathogenic influence in the gut microbiota, interactions such as microbiome-host immune system interaction and microbe-microbe interactions involved in IBD, currently available IBD therapies, followed by a detailed review on bacterial infections that might be involved in IBD, globally and specifically in India.

关键词： Ulcerative colitis Crohn’s disease Inflammatory bowel disease Gut microbiota Lactic acid bacteria