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Au,Pd and maghemite nanofunctionalized hydroxyapatite scaffolds for bone regeneration

Regenerative Biomaterials 2020年第5期7卷 461-469页

作者： giovanna calabrese Salvatore Petralia Claudia Fabbi Stefano Forte Domenico Franco Salvatore Guglielmino Emanuela Esposito Salvatore Cuzzocrea Francesco Traina Sabrina Conoci Department of Chemistry Biology Pharmacy and Environmental Science University of MessinaPiazza Pugliatti198122 MessinaSiciliaItaly Research and Development Applied Chemical WorksPaternoCataniaItaly Department of Chemical Science Fin-Ceramica FaenzaVia Granarolo 177/3Faenza 48018Italy Istituto Oncologico del Mediterraneo Ricerca ViagrandeCatania 95029Italy Dipartimento Biomorf Universita degli Studi di MessinaMessinaItaly Rizzoli Orthopedic Institute IRCSSBolognaItaly Distretto Tecnologico Micro e Nano Sistemi Sicilia CataniaItaly

Nanotechnology plays a key role in the development of innovative scaffolds for bone tissue engineering(BTE)allowing the incorporation of nanomaterials able to improve cell proliferation and *** this study,Mg-HA-Coll type I scaffolds(Mg-HA-based scaffolds)were nanofunctionalized with gold nanorods(Au NRs),palladium nanoparticles(Pd NPs)and maghemite nanoparticles(MAG NPs).Nanofunctionalized Mg-HA-based scaffolds(NF-HA-Ss)were tested for their ability to promote both the proliferation and the differentiation of adipose-derived mesenchymal stem cells(hADSCs).Results clearly highlight that MAG nanofunctionalization substantially improves cell proliferation up to 70% compared with the control(Mg-HA-based scaffold),whereas both Au NRs and Pd NPs nanofunctionalization induce a cell growth inhibition of 94% and 89%,*** evidences were found for the osteoinductive properties showing relevant calcium deposits(25% higher than the control)for MAG nanofunctionalization,while a decreasing of cell differentiation(20% lower than the control)for both Au NRs and Pd NPs *** results are in agreement with previous studies that found cytotoxic effects for both Pd NPs and Au *** excellent improvement of both osteoconductivity and osteoinductivity of the MAG NF-HA-S could be attributed to the high intrinsic magnetic field of superparamagnetic MAG *** findings may pave the way for the development of innovative nanostructured scaffolds for BTE.

关键词： bone regeneration hydroxyapatite scaffold tissue engineering gold nanoroads Pd nanoparticles maghemite nanoparticles

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Esophageal cell proliferation in gastroesophageal reflux disease: Clinical-morphological data before and after pantoprazole

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World Journal of Gastroenterology 2009年第8期15卷 936-941页

作者： Carlo calabrese Davide Treré Giuseppina Liguori Veronica Gabusi Manuela Vici giovanna Cenacchi Massimo Derenzini Giulio Di Febo Department of Clinical Medicine University of Bologna Bologna 40138 Italy Department of Experimental Pathology Clinical Pathology University of Bologna Bologna 40138 Italy Clinical Department of Radiologic and Histopathologic Sciences University of Bologna Bologna 40138 Italy

AIM: To evaluate esophageal mucosal defense mechanisms at an epithelial level to establish if pantoprazole treatment can induce ultrastructural healing and improvement in the proliferation activity of the esophageal epithelium in gastroesophageal reflux disease (GERD). METHODS: This was a single-blinded study for pHmonitoring, and histological, ultrastructural and MIB1 immunostaining evaluation. Fifty eight patients with GERD were enrolled and underwent 24 h pH-monitoring and endoscopy. Patients were treated for 12 and 24 mo with pantoprazole. Esophageal specimens were taken for histological and ultrastructural evaluation, before and after the treatment. RESULTS: With transmission electron microscopy, all patients with GERD showed ultrastructural signs of damage with dilation of intercellular spaces (DIS). After 3 mo of therapy the mean DIS values showed asignificant reduction and the mean MIB1-LI values of GERD showed an increase in cell proliferation. A further 3 mo of therapy significantly increased cell proliferation only in the erosive esophagitis (ERD) group. CONCLUSION: Three months of pantoprazole therapy induced ultrastructural healing of mucosal damage in 89% and 93% of ERD and non-erosion patients, respectively. Moreover, long-term pantoprazole treatment may be helpful in increasing the capability for esophageal cell proliferation in GERD, particularly in ERD patients.

关键词： Gastroesophageal reflux disease Esophagitis Cell proliferation Electron microscopy Pantoprazole

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Effect of omeprazole on symptoms and ultrastructural esophageal damage in acid bile reflux

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World Journal of Gastroenterology 2005年第12期11卷 1876-1880页

作者： Carlo calabrese Anna Fabbri Mauro Bortolotti giovanna Cenacchi Scialpi Carlo Desiree Zahlane Mario Miglioli Giulio Di Febo Dipartimento di Medicina Interna e Gastroenterologia

AIM: To value whether omeprazole could induce the healing of DIS and regression of symptoms in patients with DGER. METHODS: We enrolled 15 symptomatic patients with a pathological esophageal 24-h pH-metry and bilimetry. Patients underwent endoscopy and biopsies were taken from the distal esophagus. Specimens were analyzed at histology and transmission electron microscopy (TEM). Patients were treated with omeprazole 40 mg/d for 3 mo and then endoscopy with biopsies was repeated. Patients with persistent heartburn and/or with an incomplete recovery of DIS were treated for 3 more months and endoscopy with biopsies was performed. RESULTS: Nine patients had a non-erosive reflux disease at endoscopy (NERD) while 6 had erosive esophagitis (ERD). At histology, of the 6 patients with erosive esophagitis, 5 had mild esophagitis and 1 moderate esophagitis. No patients with NERD showed histological signs of esophagitis. After 3 mo of therapy, 13/15 patients (86.7%,P<0.01) showed a complete recovery of DIS and disappearance of heartburn. Of the 2 patients treated for 3 more months, complete recovery of DIS and heartburn were achieved in one. CONCLUSION: Three or 6 mo of omeprazole therapy led to a complete regression of the ultrastructural esophageal damage in 86.7% and in 93% of patients with DGER, NERD and ERD respectively. The ultrastructural recovery of the epithelium was accompanied by regression of heartburn in all cases.

关键词： Duodenogastroesophageal reflux Gastroeso phageal reflux disease Transmission Electron Microscopy Dilated Intercellular Spaces Non-erosive reflux disease

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Wireless capsule endoscopy and proximal small bowel lesions in Crohn’s disease

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World Journal of Gastroenterology 2010年第26期16卷 3299-3304页

作者： Carmelina Petruzziello Sara Onali Emma calabrese Francesca Zorzi Marta Ascolani giovanna Condino Elisabetta Lolli Paola Naccarato Francesco Pallone Livia Biancone GI Unit Department of Internal Medicine University "Tor Vergata" Rome 00133 Italy

AIM: To investigate the prevalence of proximal small bowel (SB) lesions detected by wireless capsule endoscopy (WCE) in Crohn's disease (CD). METHODS: WCE was performed in 64 patients: 32 with CD of the distal ileum, and 32 controls with iron-deficiency anemia (IDA) or diarrhea. WCE was performed using the Given SB-WCE, followed by small intestine contrast ultrasonography (SICUS). Findings compatible with CD by using WCE included erosions, aphthoid or deep ulcers, and strictures/stenosis. RESULTS: WCE detected proximal SB lesions in 16/32 (50%) patients (14 aphthoid ulcers, 2 deep ulcers, one stricture), which appeared not to be related to clinical parameters [epigastric pain, age, smoking, non-steroidalanti-inflammatory drugs (NSAIDs), IDA]. Among patients with proximal SB lesions, 6 (37%) were smokers, 3 (19%) NSAID users, 3 (19%) had epigastric pain and 4 (25%) had IDA. SICUS detected proximal SB lesions in 3/32 patients (19%) also showing lesions with WCE. No correlations were observed between proximal SB lesions assessed by WCE or by SICUS (χ2 = 1.5, P = 0.2). CONCLUSION: The use of WCE allows the detection of previously unknown upper SB lesions in a high proportion of patients with a previous diagnosis of CD involving the distal ileum.

关键词： Wireless capsule endoscopy Crohn’s disease Small bowel

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Ileal lesions in patients with ulcerative colitis after ileo-rectal anastomosis:Relationship with colonic metaplasia

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World Journal of Gastroenterology 2008年第34期14卷 5290-5300页

作者： Livia Biancone Emma calabrese Giampiero Palmieri Carmelina Petruzziello Sara Onali Giuseppe Sigismondo Sica Marta Cossignani giovanna Condino Kiron Moy Das Francesco Pallone Cattedra di Gastroenterologia Dipartimento di Medicina InternaUniversità di "Tor Vergata" Cattedra di Anatomia ed Istologia Patologica Università di "Tor Vergata" Cattedra di Gastroenterologia Dipartimento di Medicina InternaUniversità di "Tor Vergata" Cattedra di Chirurgia Generale Università di "Tor Vergata" Cattedra di Gastroenterologia Dipartimento di Medicina Interna Università di "Tor Vergata" GI Unit Department of Gastroenterology & Hepatology University of Medicine & Dentistry of New Brunswick

AIM:To assess whether in ulcerative colitis (UC) patients with ileo-rectal anastomosis (IRA), ileal lesions may develop in the neo-terminal-ileum and their possible relation with phenotypic changes towards colonic epithelium. METHODS: A total of 19 patients with IRA under regular follow up were enrolled, including 11 UC and 8 controls (6 Crohn’s disease, CD; 1 familial adenomatous polyposis, FAP; 1 colon cancer, colon K). Ileal lesions were identifi ed by ileoscopy with biopsies taken from the ileum (involved and uninvolved) and from the rectal stump. Staining included HE and immunohistochemistry using monoclonal antibodies against colonic epithelial protein CEP (Das-1) and human tropomyosin isoform 5, hTM5 (CG3). Possible relation between development of colonic metaplasia and ileal lesions was ***: Stenosing adenocarcinoma of the rectal stump was detected in 1 UC patient. The neo-terminal ileum was therefore investigated in 10/11 UC patients. Ileal ulcers were detected in 7/10 UC, associated with colonic metaplasia in 4/7 (57.1%) and Das-1 and CG3 reactivity in 3/4 UC. In controls, recurrence occurred in 4/6 CD, associated with colonic metaplasia in 3/4 and reactivity with Das-1 and CG3 in 2/3. CONCLUSION: Present fi ndings suggest that in UC, ileal lesions associated with changes towards colonic epithelium may develop also after IRA. Changes of the ileal content after colectomy may contribute to the development of colonic metaplasia, leading to ileal lesions both in the pouch and in the neo-terminal ileum after IRA.

关键词： Ulcerative colitis Ileo-rectal anastomosis Ileal lesions Colonic metaplasia

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Small intestine contrast ultrasonography vs computed tomography enteroclysis for assessing ileal Crohn's disease

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World Journal of Gastroenterology 2012年第42期18卷 6088-6095页

作者： Sara Onali Emma calabrese Carmelina Petruzziello Francesca Zorzi Giuseppe Sica Roberto Fiori Marta Ascolani Elisabetta Lolli giovanna Condino Giampiero Palmieri Giovanni Simonetti Francesco Pallone Livia Biancone Department of Systems Medicine University "Tor Vergata"00133 RomeItaly Department of Surgery University "Tor Vergata"00133 RomeItaly Radiology Unit Department of ImagingUniversity "Tor Vergata"00133 RomeItaly Pathology Unit University "Tor Vergata"00133 RomeItaly

AIM:To compare computed tomography enteroclysis(CTE) vs small intestine contrast ultrasonography(SICUS) for assessing small bowel lesions in Crohn's disease(CD),when using surgical pathology as gold ***:From January 2007 to July 2008,15 eligible patients undergoing elective resection of the distal ileum and coecum(or right colon) were prospectively *** patients were under *** study population included 6 males and 9 females,with a median age of 44 years(range:18-80 years).Inclusion criteria:(1) certain diagnosis of small bowel requiring elective ileo-colonic resection;(2) age between 18-80 years;(3) elective surgery in our Surgical Unit;and(4) written informed *** and CTE were performed ≤ 3 mo before surgery,followed by surgical *** following small bowel lesions were blindly reported by one sonologist,radiologist,surgeon and histolopathologist:disease site,extent,strictures,abscesses,fistulae,small bowel *** between findings at SICUS,CTE,surgical specimens and histological examination was made by assessing the specificity,sensitivity and accuracy of each technique,when using surgical findings as gold ***:Among the 15 patients enrolled,CTE was not feasible in 2 patients,due to urgent surgery in one patients and to low compliance in the second patient,refusing to perform CTE due to the discomfort related to the naso-jejunal *** analysis for comparing CTE vs SICUS findings was therefore performed in 13 out of the 15 CD patients *** from CTE,SICUS was feasible in all the 15 patients *** complications were observed when using SICUS or *** pathology findings in the tested population included:small bowel stricture in 13 patients,small bowel dilation above ileal stricture in 10 patients,abdominal abscesses in 2 patients,enteric fistulae in 5 patients,lymphnodes enlargement(> 1 cm) in 7 patients and mesenteric enlargement in 9 *** order to compare fi

关键词： Crohn's disease Ileal lesions Computed tomography enteroclysis Small intestine contrast ultrasonography Surgical findings

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