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Buds Reactivity and Factors Promoting Shoots Proliferation and Rooting of Cashew Seedlings Using in Vitro Tissue Culture Process

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Advances in Bioscience and Biotechnology 2022年第9期13卷 388-400页

作者： Bénédicte Sophie Adjoua Akakpo Arnaud Agbidinoukoun Corneille Ahanhanzo gilles habib todjro cacai Bienvenu Témidouan Badou Serge Houedjissin Joseph Dossou Central Laboratory of Plant Biotechnology and Plant Breeding Faculty of Sciences and Technique University of Abomey-Calavi (UAC) Cotonou Benin Faculty of Agronomic Sciences University of Abomey-Calavi Cotonou Benin

Tissue culture techniques are widely used for the mass propagation of many species. In cashew in vitro propagation, some protocols need to be established at this end. The present work was carried out to evaluate the conditions for in vitro regeneration of cashew seedlings from micropropagation by organogenesis on Benin genotypes. Nodal explants from one-month-old cashew seedlings in the greenhouse and cotyledonary nodes from in vitro germination were used for this purpose. BAP and kinetin were evaluated alone at 2.2 mg/L and then the combination of 2.2 mg/L BAP + 0.2 mg/L IBA was also evaluated. The response of axillary bud proliferation on explants was obtained with both cotyledonary nodes and axillary buds from different combinations of growth regulators. However, the best responses were recorded with cotyledonary nodes. When 2.2 mg/L BAP was used, 80% of the explants responded with numerous proliferation (5 to 8) buds (5.75 ± 0.12) with good shoot length (6.73 ± 0.3 cm) on MS medium containing 150 mL coconut water. Rooting was observed with the combination of NAA (2.5 mg/l) + IBA (2.5 mg/l) on 1/2 MS containing 40 g/l sucrose.

关键词： Micropropagation Cashew Elite Genotypes Buds In Vitro Regeneration Organogenesis

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Effects of Non-Conventional Substrate and NPK Fertilizer on Cassava Plantlets Acclimatization for Plant Material Production

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Agricultural Sciences 2021年第10期12卷 1058-1069页

作者： gilles habib todjro Cacaï Corneille Ahanhanzo Dèwanou Kant David Ahoya Serge Sètondji Houédjissin Jerome Anani Houngue Central Laboratory of Plant Biotechnology and Plant Breeding Department of Genetics and Biotechnology Faculty of Science and Technology University of Abomey-Calavi Cotonou Benin

The adaptation of cassava plantlets to natural conditions remains the main obstacle in the process of free virus plantlets production. In this study, cassava plantlets acclimatization was assessed by pre-acclimatization, height of acclimated plantlets, substrate, and nutrient watering solution. The results revealed that pre-acclimatization gave a high survival rate (61.9%) whereas the direct tunnel acclimatization was conducted to low rate (27.31%) of plantlets. Furthermore, the height of plantlets influenced significantly (p < 0.05) their survival rate with best survival rate observed on plantlet height from 1 cm to 5 cm. There was no significant difference between the substrates used and watering solutions. However, the Sawdust substrate gave a high survival rate (46.67%) and better phylogenesis (1.8) in the variety RB 89509. Likewise, plantlets treated with NPK nutrient watering solutions (20-10-10) had a better survival rate (48.57%), better phylogenesis (2.125) with the variety RB 89509 and better growth in height (1.18 cm) with the variety BF92/0267. These findings constitute a database for the establishment of the technical itinerary for the acclimatization of cassava plantlets.

关键词： Manihot esculenta Plantlets Pre-Acclimatization Substrates Nutrient Solution NPK

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Implementation of the Acclimatization Process for Ginger Plantlets (Zingiber officinale Roscoe)

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Open Journal of Applied Sciences 2024年第8期14卷 2236-2248页

作者： Florentin Danton Kango gilles habib todjro Cacaï Serge Sètondji Houédjissin Jerome Anani Hounguè Jaures Riwan Uriel Kouke David Dègbé Allotchémè Corneille Ahanhanzo Central Laboratory of Plant Biotechnology and Plant Breeding Department of Genetic and Biotechnology Faculty of Science and Technology University of Abomey-Calavi Cotonou Benin

Ginger (Zingiber officinale Roscoe) is an important culinary and medicinal spice but is rarely cultivated due to the unavailability of seeds. Given the difficulties in adapting to plantlets produced in natural environments, it is important to analyze the survival conditions of ginger plantlets. For this reason, we varied the incubation temperature and humidity as well as the substrate during the weaning phase. Then, we varied the nutrients contained in the watering solution during the hardening phase. The statistical analysis showed that physical factors and substrates significantly influenced (p < 0.0001) plantlet survival. Nutrient solutions significantly influenced the phylogenesis, rhizogenesis, and height growth of the plantlets. The suitable physical factors for good development of plantlets are a temperature of 26.54˚C and a humidity of 96.16%. The 1C2T2TC substrate (1 Compost + 2 Soil + 2 Coconut Peat) had a significant survival rate of approximately 92.5%. During hardening, the Plantzym solution promoted good growth in terms of plantlet height (0.6 cm) and good development of roots (30 roots) and leaves (03 leaves). This work will make it possible to develop a technical seed production sheet for better development of ginger cultivation in Benin.

关键词： Acclimatization Zingiber officinale Weaning Nutrient Solutions Substrates

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