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Stability of PAC(Psy-2A-Crt I) gene and agronomic traits in the F_(2:3) of IR36/PAC transgenic plants

Journal of Integrative Agriculture 2015年第6期14卷 1163-1170页

作者： Hyun Suk Lee gihwan yi Kyung Min Kim Division of Plant Biosciences School of Applied Biosciences College of Agriculture and Life Science Kyungpook National University Department of Farm Management College of Agriculture and Life Science Kyungpook National University

This study was carried out to evaluate the expression of yellow endosperm color in the progeny of Psy-2A-Crt I（β-carotene gene, PAC） transgenic plants crossed with IR36. The selected 25 lines of F2 progeny exhibited stable expression of yellow endosperm color and high agronomic characteristics. IR36 and PAC transgenic plant of the grain length, width and ratio showed the same tendency of normal distribution. For progeny selection, colorimeters were as employed to distinguish differing visible colors. Correlation analysis was conducted to investigate the relationship between b＊ value and the expression of specific genes. b＊ value was associated with the relative expression of the PAC-Rev and MAR product by real-time PCR and t-test revealed significant difference. Based on this study, correlation between colorimetric values and real-time PCR was effective to detect gene expression. Yellow endosperm progenies represent a new genetic pool that might be useful to increase the genetic diversity of yellow endosperm rice.

关键词： transgenic agronomic crossed yellow breeding panicle length distinguish phenotype Correlation

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Analysis of Low Amylose and Processability Fractured Endosperms Derived from Somatic Variation

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Food and Nutrition Sciences 2013年第6期4卷 21-27页

作者： gihwan yi Kyung Min Kim Department of Farm Management College of Agriculture & Life Science Kyungpook National University Gunwi-gun South Korea Division of Plant Biosciences School of Applied Biosciences College of Agriculture and Life Science Kyungpook National University Daegu South Korea.

Recently, improving eating quality and processing properties has become one of the most important objectives in japonica rice breeding programs in Korea. This study was carried out to determine the agronomy and physicochemical characteristics of the opaque endosperm rice "S-21-3-8" regenerated from seed-derived callus culture of a rice cultivar, "Ilpum". S3 generation of opaque endosperm mutants selected from pedigree breeding was used for analysis of agronomic and physicochemical traits. Genetic segregation was observed at the highest frequency among opaque endosperm mutants, being present in 85.7% (12/14 lines) of the entire opaque lines. However, the major agricultural characteristics and grain traits of "S-21-3-8" were similar to those of a donor cultivar, "Ilpum". "S-21-3-8" showed significantly lower (10.6%) amylose than those (17.7%) of "Ilpum" in brown rice, while the protein levels were similar to those of the donor plant. The grain hardness of "S-21-3-8" (1.67 kgf/mm2) was lower than that of "Ilpum" (1.97 kgf/mm2), resulting in a high flour-milling percentage. The loosely packed starch granules of "S-21-3-8" in the opaque endosperm were demonstrated by SEM analysis of cross-sectioned rice grains. The opaque endosperm mutants that were of somaclonal variations in the tissue culture will lead to improved eating quality and processing properties of rice.

关键词： Rice Opaque Endosperm Flour-Milling Starch Granules

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Analysis of Genetic and Biogeography Diversity of Korean Indigenous Wild Rocambole (<i>Allium monanthum</i>Max)

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American Journal of Plant Sciences 2013年第5期4卷 1054-1059页

作者： gihwan yi Kyung-Min Kim Division of Agroindustry & Farm Management Kyungpook National University Daegu South Korea Division of Plant Biosciences School of Plant Biosciences Kyungpook National University Daegu South Korea.

This study was conducted to obtain baseline data describing Allium monanthum Max. for breeding an elite cultivar. A. monanthum has recently become popular vegetable in Korea owing to its unique flavors and nutraceutical effects. The agronomic traits of 271 accessions of A. monanthum collected throughout Korea were evaluated in this study. Leaf width showed the greatest variation (0.1 to 25 mm) among traits evaluated, followed by leaf sheath length and number of cormlets. The average leaf sheath length and diameter, which are commonly used to evaluate quality in the market, were 1.29 cm and 0.3 cm. Some superior accessions had a leaf sheath length of >10 cm and diameter of >1.1 cm. The average weight of the cormlets ranged from 1 to 2 g;however, one accession weighed more than 4 g. The genetic diversity of A. monanthum calculated based on morphology and molecular distance was greater in Jeolla and Gyeoungsang provinces than Gyeonggi and Chungcheong provinces. Finally, seven elite accessions of A. monanthum were selected owing to their potential usefulness in future breeding projects.

关键词： Allium monanthum Indigenous Agronomic Traits Elite Lines Breeding

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