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Small intestine contrast ultrasonography vs computed tomography enteroclysis for assessing ileal Crohn's disease

World Journal of Gastroenterology 2012年第42期18卷 6088-6095页

作者： Sara Onali Emma Calabrese Carmelina Petruzziello Francesca Zorzi Giuseppe Sica Roberto Fiori Marta Ascolani Elisabetta Lolli Giovanna Condino giampiero palmieri Giovanni Simonetti Francesco Pallone Livia Biancone Department of Systems Medicine University "Tor Vergata"00133 RomeItaly Department of Surgery University "Tor Vergata"00133 RomeItaly Radiology Unit Department of ImagingUniversity "Tor Vergata"00133 RomeItaly Pathology Unit University "Tor Vergata"00133 RomeItaly

AIM:To compare computed tomography enteroclysis(CTE) vs small intestine contrast ultrasonography(SICUS) for assessing small bowel lesions in Crohn's disease(CD),when using surgical pathology as gold standard.METHODS:From January 2007 to July 2008,15 eligible patients undergoing elective resection of the distal ileum and coecum(or right colon) were prospectively enrolled.All patients were under follow-up.The study population included 6 males and 9 females,with a median age of 44 years(range:18-80 years).Inclusion criteria:(1) certain diagnosis of small bowel requiring elective ileo-colonic resection;(2) age between 18-80 years;(3) elective surgery in our Surgical Unit;and(4) written informed consent.SICUS and CTE were performed ≤ 3 mo before surgery,followed by surgical pathology.The following small bowel lesions were blindly reported by one sonologist,radiologist,surgeon and histolopathologist:disease site,extent,strictures,abscesses,fistulae,small bowel dilation.Comparison between findings at SICUS,CTE,surgical specimens and histological examination was made by assessing the specificity,sensitivity and accuracy of each technique,when using surgical findings as gold standard.RESULTS:Among the 15 patients enrolled,CTE was not feasible in 2 patients,due to urgent surgery in one patients and to low compliance in the second patient,refusing to perform CTE due to the discomfort related to the naso-jejunal tube.The analysis for comparing CTE vs SICUS findings was therefore performed in 13 out of the 15 CD patients enrolled.Differently from CTE,SICUS was feasible in all the 15 patients enrolled.No complications were observed when using SICUS or CTE.Surgical pathology findings in the tested population included:small bowel stricture in 13 patients,small bowel dilation above ileal stricture in 10 patients,abdominal abscesses in 2 patients,enteric fistulae in 5 patients,lymphnodes enlargement(> 1 cm) in 7 patients and mesenteric enlargement in 9 patients.In order to compare findings by using SICUS,CTE,histology and surgery,

关键词：计算机断层扫描技术评估小肠回肠病理组织学检查灌肠造影超声

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Ileal lesions in patients with ulcerative colitis after ileo-rectal anastomosis:Relationship with colonic metaplasia

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World Journal of Gastroenterology 2008年第34期14卷 5290-5300页

作者： Livia Biancone Emma Calabrese giampiero palmieri Carmelina Petruzziello Sara Onali Giuseppe Sigismondo Sica Marta Cossignani Giovanna Condino Kiron Moy Das Francesco Pallone Cattedra di Gastroenterologia Dipartimento di Medicina InternaUniversità di "Tor Vergata" Cattedra di Anatomia ed Istologia Patologica Università di "Tor Vergata" Cattedra di Gastroenterologia Dipartimento di Medicina InternaUniversità di "Tor Vergata" Cattedra di Chirurgia Generale Università di "Tor Vergata" Cattedra di Gastroenterologia Dipartimento di Medicina Interna Università di "Tor Vergata" GI Unit Department of Gastroenterology & Hepatology University of Medicine & Dentistry of New Brunswick

AIM:To assess whether in ulcerative colitis (UC) patients with ileo-rectal anastomosis (IRA), ileal lesions may develop in the neo-terminal-ileum and their possible relation with phenotypic changes towards colonic epithelium. METHODS: A total of 19 patients with IRA under regular follow up were enrolled, including 11 UC and 8 controls (6 Crohn’s disease, CD; 1 familial adenomatous polyposis, FAP; 1 colon cancer, colon K). Ileal lesions were identifi ed by ileoscopy with biopsies taken from the ileum (involved and uninvolved) and from the rectal stump. Staining included HE and immunohistochemistry using monoclonal antibodies against colonic epithelial protein CEP (Das-1) and human tropomyosin isoform 5, hTM5 (CG3). Possible relation between development of colonic metaplasia and ileal lesions was investigated.RESULTS: Stenosing adenocarcinoma of the rectal stump was detected in 1 UC patient. The neo-terminal ileum was therefore investigated in 10/11 UC patients. Ileal ulcers were detected in 7/10 UC, associated with colonic metaplasia in 4/7 (57.1%) and Das-1 and CG3 reactivity in 3/4 UC. In controls, recurrence occurred in 4/6 CD, associated with colonic metaplasia in 3/4 and reactivity with Das-1 and CG3 in 2/3. CONCLUSION: Present fi ndings suggest that in UC, ileal lesions associated with changes towards colonic epithelium may develop also after IRA. Changes of the ileal content after colectomy may contribute to the development of colonic metaplasia, leading to ileal lesions both in the pouch and in the neo-terminal ileum after IRA.

关键词：溃疡性结肠炎肠阻塞回盲肠吻合术结肠组织转化

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Intestinal duplication in adulthood: A rare entity, difficult to diagnose

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World Journal of Gastrointestinal Surgery 2011年第8期3卷 128-130页

作者： Cristina Fiorani Rosa Scaramuzzo Alessandra Lazzaro Livia Biancone giampiero palmieri Achille L Gaspari Giuseppe Sica Department of Surgical Science Tor Vergata University Hospital Department of Medicine Gastroenterology Unit Tor Vergata University Hospital Department of Pathology Tor Vergata University Hospital

Duplications of the alimentary tract (ATD) are rare congenital anomalies often found early in life. They may occur anywhere in the intestinal tract but the ileum is the most frequently affected site. Clinical presentation of ATD in adults is variable and because these lesions occur so infrequently they are rarely suspected. In the present report we describe a case of ileal duplication in a 61-year-old patient with Crohn’s disease. Despite various radiological investigations and medical consultations, the diagnosis was only made on the surgical specimen.

关键词： Intestinal duplication Adulthood Intestinal obstruction Surgical resection Abdominal pain

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Evanescent waveguide lab-on-chip for optical biosensing in food quality control

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Photonics Research 2022年第6期10卷 1453-1461页

作者： ALESSIO BUZZIN RITA ASQUINI DOMENICO CAPUTO giampiero DE CESARE Department of Information Engineering Electronics and TelecommunicationsSapienza University of Rome00184 RomeItaly

Optical biosensing systems are commonly developed assembling a source,a light–sample interaction area,and a detector as distinct stand-alone elements.We present a compact,inexpensive,and easy-to-use glass chip that monolithically integrates both the interaction and detection elements in a 1 cm^(2)overall surface.The device working principle is based on evanescent wavelight interaction with the complex refractive index of a liquid mixture,being the index influenced by the mixture’s physical and chemical features.We describe the manufacture of a prototype able to perform investigations on food quality and subsequent tests on the detection of fat content in milk.Theoretical investigations are reported as well as measurements performed on samples in the green spectrum.A sensitivity of about 139 fA/(g/d L)and a limit of detection of 14 ppm have been achieved,better than those of current commercial devices.

关键词： waveguide optical interaction

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Organelle trafficking, the cytoskeleton, and pollen tube growth

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Journal of Integrative Plant Biology 2015年第1期57卷 63-78页

作者： giampiero Cai Luigi Parrotta Mauro Cresti Department of Life Sciences University of Siena

The pollen tube is fundamental for the reproduction of seed plants. Characteristically, it grows relatively quickly and uni-directionally （＂polarized growth＂） to extend the male gametophyte to reach the female gametophyte. The pollen tube forms a channel through which the sperm cells move so that they can reach their targets in the ovule. To grow quickly and directionally, the pollen tube requires an intense movement of organelles and vesicles that allows the cell＇s contents to be distributed to sustain the growth rate. While the various organelles distribute more or less uniformly within the pollen tube, Golgi-released secretory vesicles accumulate massively at the pollen tube apex, that is, the growing region. This intense movement of organelles and vesicles is dependent on the dynamics of the cytoskeleton, which reorganizes differentially in response to external signals and coordinates membrane trafficking with the growth rate of pollen tubes.

关键词： Actin filaments cytoplasmic streaming microtubules motor proteins tip growth

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Fitness Cost Shapes Differential Evolutionary Dynamics of Disease Resistance Genes in Cultivated and Wild Plants

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Molecular Plant 2020年第10期13卷 1352-1354页

作者： Delfina Barabaschi Alessandro Tondelli giampiero Valè Luigi Cattivelli CREA Research Centre for Genomics and Bioinformatics Via San Protaso 302Fiorenzuola d'Arda29017Italy DiSIT Dipartimento di Scienze e Innovazione TecnologicaUniversity of Piemonte OrientalePiazza S.Eusebio 5Vercelli 13100Italy

Polyploidy is characterized by the presence of two or more complete sets of chromosomes coming from intraspecies whole-genome duplication(i.e.,autopolyploidy)or from hybridization between distinct but related species(i.e.,allopolyploidy).Poly-ploidization is a driving force in the evolution of higher plants,most of the plant species have experienced ancient or recent genome duplication events that have led to several genome modifications,such as chromosomal reorganization,gene conversion,sub-genome dominance,alteration of gene expression patterns,and expansion/contraction of some gene families.

关键词： chromosomal contraction driving

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Kinetics of defect formation in chemically vapor deposited （CVD） graphene during laser irradiation： The case of Raman investigation

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Nano Research 2015年第12期8卷 3972-3981页

作者： giampiero Amato Gianluca Milano Umberto Vignolo Ettore Vittone Quantum Research Laboratory INRIM Strada delle Cacce 91 Torino Italy Department of Physics NIS Research Center University of Torino via Pietro Giuria 1 10125 Torino Italy

激光照耀在上的效果化学上，蒸汽扔了(CVD ) graphene 被分析在各种各样的照明条件下面获得的拉曼系列的时间的进化学习。系列证明缺点相关的山峰的规范的紧张与暴露时间的方形的根增加并且与激光力量密度几乎线性地变化。而且，到放射... 详细信息

激光照耀在上的效果化学上，蒸汽扔了(CVD ) graphene 被分析在各种各样的照明条件下面获得的拉曼系列的时间的进化学习。系列证明缺点相关的山峰的规范的紧张与暴露时间的方形的根增加并且与激光力量密度几乎线性地变化。而且，到放射损坏的 graphene 的坚硬取决于它的内在的结构的质量。结果建议与普通信仰相反， micro-Raman 光谱学不能为 graphene 的描述被认为一个 noninvasive 工具。试验性的观察与我们在 hydrogenated 从 StaeblerWronski 效果的解释途径导出的一个模型兼容非结晶的硅；这条途径假设 photoexcited 搬运人的再结合导致碎弱 CC 契约。

关键词：缺陷形成激光辐照化学气相沉积石墨动力学显微拉曼光谱激光功率密度氢化非晶硅

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New device to implement the adenoma detection rate

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World Journal of Clinical Cases 2017年第7期5卷 258-263页

作者： Maddalena Zippi Wandong Hong Pietro Crispino giampiero Traversa Unit of Gastroen- terology and Digestive Endoscopy Sandro Pertini Hospital Department of Gastroenterology and Hepato- logy First Affiliated Hospital of Wenzhou Medical University Unit of Medicine and Urgency San Giovanni Hospital

It is well-known that colonoscopy is considered the gold standard for colon cancer prevention.Although performed by experienced endoscopists,the matter remains of polyps missed during this examination.The reasons may include the size,shape and location of the lesions.Many colorectal cancer screening programs have been proposed to increase the adenoma detection rate.The substantial difference between these methods is whether the improvement in vision,particularly the detection of irregularities of the mucosa,is inside the endoscope electronic components(magnification,wideangle vision,narrow band imaging,flexible spectral imaging colour enhancement,i-Scan) or outside the same,by the use of specific caps(EndoC uff,EndoV ision,Endo Rings).Endocuff is a plastic device mounted at the end of the scope with a constant vision field of the entire colon.The aim of this study is to explore the potential clinical and technical benefits of Endocuff.

关键词： Adenoma detection rate Cap-assisted colonoscopy Colorectal cancer Endocuff-assisted colonoscopy Standard colonoscopies

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Congruent responses of vascular plant and ant communities to pastoral land-use abandonment in mountain areas throughout different biogeographic regions

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Ecological Processes 2022年第1期11卷 469-482页

作者： Marcello Verdinelli Marco Pittarello Maria Carmela Caria Giovanna Piga Pier Paolo Roggero Gian Marco Marrosu Stefano Arrizza Maria Leonarda Fadda giampiero Lombardi Michele Lonati Ginevra Nota Maria Sitzia Simonetta Bagella Institute of BioEconomy(CNR IBE) National Research Council of Italy07100 SassariItaly Department of Agricultural Forestand Food Science(DIS-AFA)University of TorinoTorinoItaly Department of Chemistry and Pharmacy University of Sassari07100 SassariItaly Department of Agricultural Sciences University of Sassari07100 SassariItaly Desertification Research Center University of Sassari07100 SassariItaly AGRIS Sardegna Loc.Bonassai07100 SassariItaly

Background:There is a long-term trend towards the abandonment of agro-pastoral activities in the mountain areas of Europe:the following encroachment process of semi-natural grasslands by shrubs is one of the main severe threats to the conservation of biodiversity in mountain environments.To better understand the impact of land abandonment,we analysed the reliability of plant functional groups,ant traits,and ant functional groups as indicators of land use changes.We carried out the research in Italy at four sites along a latitudinal/altitudinal gradient in three biogeo-graphic regions(Mediterranean,Continental,Alpine).We identified three stages of a chronosequence at each site as representative of the plant succession in response to pastoral land-use abandonment.Results:As expected,both the plant and ant assemblages considerably differed across sites at the species level and,within each site,among the three stages.This trend was found also using ant traits,functional groups of ants,and plant functional groups.Ant and plant communities were related in terms of composition and functionality.Harvester ants and ants with collective foraging strategy were associated with annual legumes and grasses(Therophytes);ants with a strictly individual foraging strategy with Phanerophytes.Ant traits and plant functional groups indicated significant differences among the three stages of the chronosequence.However,ant functional groups could not discriminate between the stages represented by secondary grasslands currently grazed and shrub-encroached grasslands ungrazed.Conclusion:Despite some limitations of ant functional groups in explaining the succession stages of land abandonment,our results suggest that ants are a good surrogate taxon and might be used as bioindicators of land-use changes and ecosystem functioning.Furthermore,our findings indicate that the functional group approach should be applied to other European ecosystems.Finally,reducing the taxonomic complexity could contribute to developing predictive models to detect

关键词： Biodiversity conservation Bioindicators Cross-taxon congruence Functional groups Land abandonment Monitoring Mountain pastures

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Dyspnea and Fatigue Following Video-assisted Thoracic Surgery for Pulmonary Lobectomy： Measuring Scales to Optimize the Allocation of Resources

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Chinese Medical Journal 2016年第17期129卷 2139-2140页

作者： Massimiliano Polastri Andrea Dell'Amore giampiero Dolci Niccolo Daddi Medicat Department of Continuity of Care and Disability Physical Medicine and Rehabilitation University Hospital St. Orsola-Malpighi Bologna 40138 Italy Department of Cardiac-Thoracic and Vascular Diseases Thoracic Surgery University Hospital St. Orsola-Malpighi Bologna 40138 Italy

To the Editor： The efficacy of minimally invasive techniques for pulmonary lobectomy is gaining a great interest in literature. Recently, new surgical strategies and technical improvements have been reported for video-assisted thoracic surgery （VATS）. Furthermore, postoperative physiotherapeutic aspects are a source of interest; it has been observed that the walking distance, calculated as the mean score on the 6-min walk test （6MWT） preoperatively, represents a predictive measure for the onset of possible postoperative complications following pulmonary lobectomy. Nevertheless, the suitability of the 6MWT during the early postoperative period is limited by common functional limitations that are normally present after surgery, including the possible need for oxygen supplementation during the first days postoperatively and the presence of thoracic drainage.

关键词： Fatigue Following Video-assisted Thoracic Surgery Pulmonary Lobectomy

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