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Effect of Marsdenia tenacissima extract on G2/M cell cycle arrest by upregulating 14-3-3σ and downregulating c-myc in vitro and in vivo

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Chinese Herbal Medicines 2019年第2期11卷 169-176页

作者： Li Sun Qurat UI Ain Ying-sheng Gao ghulam Jilany Khan Sheng-tao Yuan Debmalya Roy Jiangsu Key Laboratory of Drug Saeening China Pharmaceutical University Nanjing 210009 China Jiangsu Center for Pharmacodynamics Research and Evaluation China Pharmaceutical University Nanjing 210009 China

Objective: Marsdenia tenacissima extract(MTE) is a traditional Chinese herbal medicine with anti-cancer activity. In some previous studies, different mechanism actions of the anti-cancer effect of MTE have been revealed. In this study, we first observed that MTE exhibited G2/M cell cycle arrest on two different human breast cancer cell lines, MDA-MB-231 and MCF-7 by mediating 14-3-3σ and ***: The effect of MTE on G2/M cell cycle arrest was evaluated in MDA-MB-231 and MCF-7 cell lines. MTT assay was done for evaluation of cell viability. Flow cytometry was employed for cell cycle analysis. Western blotting analysis and immunohistochemistry were performed to analyze the expression of G2/M cell cycle-related key protein in cells and tissue samples. Animal studies have been conducted to elucidate the anti-tumor effect of ***: Cell cycle is the backbone for developing cancer. Cell cycle proteins play a major role in the progression of cell cycle and cell proliferation. However, some key protein directly or indirectly modulate the action of cell cycle protein that highly affect cell cycle regulation. In order to investigate cellular proliferation of cancer, we observed that MTE induced the upregulation of 14-3-3σ and downregulation of c-myc,and then reduced the expression of G2/M cell cycle associated key protein, leading to the inhibition of cellular entry into mitosis phase. We also confirmed that MTE exerted a significant antitumor effect on the MDA-MB-231 xenograft model in ***: G2/M cell cycle arrest occurred by the action of MTE, mediated by the upregulation of 14-3-3σ as well as downregulation of c-myc in MDA-MB-231 and MCF-7 cell lines.

关键词： c-myc G2/M arrest Marsdenia tenacissima extract MCF-7 MDA-MB-231 14-3-3σ

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Effect of Tillage and Nitrogen on Wheat Production, Economics, and Soil Fertility in Rice-Wheat Cropping System

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American Journal of Plant Sciences 2013年第1期4卷 17-25页

作者： Khalid Usman Ejaz Ahmad Khan Niamatullah Khan Muhammad Anwar Khan Said ghulam Sarfaraz Khan Jalaluddin Baloch Department of Agronomy Faculty of Agriculture Gomal University Dera Ismail Khan Pakistan Department of Horticulture Faculty of Agriculture Gomal University Dera Ismail Khan Pakistan Department of Soil & Environmental Sciences Faculty of Agriculture Gomal University Dera Ismail Khan Pakistan University Wensam College Gomal University Dera Ismail Khan Pakistan

Conservation tillage and nitrogen may improve soil fertility, yield and income on sustainable basis. The aim of this study was to evaluate the impact of three tillage systems viz. zero (ZT), reduced (RT), and conventional tillage (CT) and five N rates (0, 80, 120, 160, and 200 kg·N·ha-1) on yield and yield components, soil organic matter (SOM), total soil N (TSN), and income of wheat (Triticum aestivum L.) grown after rice (Oryza sativa L.). Nitrogen rates significantly affected yield and yield attributing characters with highest values recorded at200 kg·N·ha-1. Mean maximum grain yield (6390 -6845 kg·ha-1), net benefit (1359 - 1460 US$ ha-1), and benefit cost ratio (5.6 - 5.7) could be achieved at 160 to200 kg·N·ha-1. The tillage systems produced similar yield and yield components except spikes m-2. The SOM and TSN were highest in ZT compared to other tillage systems. Though tillage × N interactions were not significant for most of the parameters under study, the overall effect of ZT with200 kg·N·ha-1 appeared to be most favorable compared to RT and CT. Zero tillage resulted in highest number of spikes m-2 (304.4), SOM (9.6 g·kg-1) and TSN (0.5 g·kg-1) with200 kg·N·ha-1. The results suggest that ZT with200 kg·N·ha-1 was optimum and sustainable strategy to achieve higher yield and income and also to improve SOM and TSN on silty clay soil.

关键词： Wheat Tillage Nitrogen Income SOM TSN

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Preparation and Characteristic of Self-floating Silica

Preparation and Characteristic of Self-floating Silica

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作者：古拉姆（ghulam Muhyodin）北京化工大学

学位级别：硕士

本文通过一种简便且经济的溶胶-凝胶法研究自漂浮疏水性二氧化硅颗粒。该方法是通过发生水解和缩合反应建立的,其中原硅酸乙酯(TEOS)和合成产物(十八烷基2-((3-(三乙氧基硅烷基)丙基)氨基)乙酸酯)用作引发剂,用不同比例的TEOS:SP合成二... 详细信息

本文通过一种简便且经济的溶胶-凝胶法研究自漂浮疏水性二氧化硅颗粒。该方法是通过发生水解和缩合反应建立的,其中原硅酸乙酯(TEOS)和合成产物(十八烷基2-((3-(三乙氧基硅烷基)丙基)氨基)乙酸酯)用作引发剂,用不同比例的TEOS:SP合成二氧化硅颗粒。在玻璃基板上以羟基-2-甲基-1-苯基-1-丙酮(1173))为光引发剂,液体中的二氧化硅颗粒和粉末二氧化硅颗粒以不同重量百分比在聚乙二醇二丙烯酸酯中进行光聚合(UV-365,光强为22 mW/cm 2下反应60s),在这个过程中,大部分二氧化硅经历了自浮,但有一点残留在下面。已证实TEOS与SP的比例对该聚合物膜的疏水性,粗糙度和光泽性质具有高度影响。已经发现,无论使用液体二氧化硅颗粒或者二氧化硅粉末颗粒,1:5比例时聚合物薄膜的疏水性和粗糙度最大,光泽性最小。因此所有的测试结果都是使用液体中的二氧化硅。0.5-3 wt%的离心二氧化硅薄膜的光泽性比液体二氧化硅颗粒的光泽性高,超过该范围直至5 wt%时,光泽度下降甚至比液体二氧化硅颗粒的光泽度更小。进行FTIR研究以鉴定所制备材料中的各种官能团,然后分别进行TEM和SEM以研究粒度和表面形态。结果表明,TEOS:SP比例的影响是非常显着的,其中1:5比率时有利于产生最大自浮性,疏水性和粗糙度以及最小光泽性的二氧化硅。该报告的目的是提供疏水性自浮二氧化硅的完整科学进展,以评估其未来展望。该报告对二氧化硅的影响进行了讨论,说明了复合材料在环境和工程方面的影响,和这个领域的快速发展的前景。

关键词：溶胶-凝胶法悬浮二氧化硅光引发聚合水接触角

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Muon flux measurement at China Jinping Underground Laboratory

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Chinese Physics C 2021年第2期45卷 509-517页

作者：郭子溢 Lars Bathe-Peters 陈少敏 Mourad Chouaki 窦威郭磊 ghulam Hussain 李进京刘倩罗光骆文泰祁鸣邵文辉唐健万林焱王喆续本达徐彤徐蔚然杨玉梓 Minfang Yeh 赵林 Department of Engineering Physics Tsinghua UniversityBeijing 100084China Center for High Energy Physics Tsinghua UniversityBeijing 100084China Key Laboratory of Particle&Radiation Imaging(Tsinghua University) Ministry of EducationChina Institut für Physik Technische Universität BerlinBerlin 10623Germany École Polytechnique Fédérale de Lausanne Lausanne 1015Switzerland School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China School of Physics Sun Yat-Sen UniversityGuangzhou 510275China School of Physics Nanjing UniversityNanjing 210093China Brookhaven National Laboratory UptonNew York 11973USA

China Jinping Underground Laboratory(CJPL)is ideal for studying solar,geo-,and supernova neutrinos.A precise measurement of the cosmic-ray background is essential in proceeding with R&D research for these MeV-scale neutrino *** a 1-ton prototype detector for the Jinping Neutrino Experiment(JNE),we detected 264 high-energy muon events from a 645.2-day dataset from the first phase of CJPL(CJPL-I),reconstructed their directions,and measured the cosmic-ray muon flux to be (3.53±0.22_stat.±0.07_sys.)×-10^(-10)cm^(-2).The observed angular distributions indicate the leakage of cosmic-ray muon background and agree with simulation data accounting for Jinping mountain's terrain.A survey of muon fluxes at different laboratory locations,considering both those situated under mountains and those down mine shafts,indicates that the flux at the former is generally a factor of (4±2) larger than at the latter,with the same vertical *** study provides a convenient back-of-the-envelope estimation for the muon flux of an underground experiment.

关键词： CJPL cosmic-ray muon flux angular distribution neutrino detector liquid scintillator

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The Implication of Blockchain in Quality Control of Automobile Spare Parts Supply Chain Case Study of SHACMAN on Counterfeit Spare Parts

The Implication of Blockchain in Quality Control of Automobi...

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作者：古拉姆（ghulam Mujtaba）长安大学

学位级别：硕士

研究目的:本文目的是探索区块链在供应链中的应用潜力,将区块链进一步划分成特定子类别、流程和点,以帮助供应链实现(敏捷性、结构灵活性、扩展企业和风险管理)高效且有效的信息管理。研究方法:通过阅读文献与研究案例(超过24个案例研究... 详细信息

研究目的:本文目的是探索区块链在供应链中的应用潜力,将区块链进一步划分成特定子类别、流程和点,以帮助供应链实现(敏捷性、结构灵活性、扩展企业和风险管理)高效且有效的信息管理。研究方法:通过阅读文献与研究案例(超过24个案例研究)建立相应理论,然后通过研究这些案例分析区块链应用在供应链中所能带来的好处。在有效分析了案例研究后,通过问卷调查和深度访谈对SHACMAN(陕西汽车控股集团有限公司)的供应链设计进行了讨论。这些案例的研究表明,区块链的应用可以实现降低供应链断链风险、提高供应链效率与降低成本的目标。研究结果:区块链的优势是可以通过合作者之间进行支持数据的一致性检验以实现分散风险,通过加密技术提高安全性能,这种优势使其成为供应链集成存储管理的完美选择。此外,它可以在整个供应链中解决一系列问题,这使其成为解决全球供应链风险的最佳选择。实践意义:全球供应链中的风险是多维的,因此解决方案应是多维的。区块链可以提供给遍布在不同地区的合作伙伴一个连接网络,使这些合作伙伴可以实现透明化、可见的、可追溯、未扭曲的和未经更新的信息管理。所有这些优势都可以解决造假问题(包括在药房、咖啡厅、汽车到飞机乃至军用备件等)、提高效率、降低风险、降低成本与可审计性。本文创新与不足:在作者的最大研究范围内,无法穷尽讨论区块链对供应链所有不同部门的影响,与区块链相关的文献缺乏对区块链实践应用的讨论及模型。此外,尚未从道德管理角度出发讨论区块链的透明性。基于区块链在供应链应用背景下,尚未对任务与技术的契合性进行一般的理论。

关键词：供应链管理区块链质量控制伪造

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Sewage Sludge: An Important Biological Resource for Sustainable Agriculture and Its Environmental Implications

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American Journal of Plant Sciences 2012年第12期3卷 1708-1721页

作者： Khalid Usman Sarfaraz Khan Said ghulam Muhammad Umar Khan Niamatullah Khan Muhammad Anwar Khan Shad Khan Khalil Department of Agriculture Chemistry Faculty of Agriculture Gomal University Dera Ismail Khan Pakistan Department of Agronomy Agricultural University Peshawar Pakistan Department of Agronomy Faculty of Agriculture Gomal University Dera Ismail Khan Pakistan Department of Soil Science Faculty of Agriculture Gomal University Dera Ismail Khan Pakistan University Wensam College Gomal University Dera Ismail Khan Pakistan

Intensive farming generally needs large addition of organic matter to maintain fertility and enhance crop yields. Sewage sludge/biosolids are by-products of municipal and industrial wastewater treatment and a rich source of organic nutrients. Sewage sludge having high content of organic matter, macro- and micro-nutrients, can be used as fertilizer/soil conditioner for food, vegetable crop, horticultural plants and pasture, which in most cases can be beneficially recycled. In the past sewage sludge was regarded as a waste product due to expected high level of contaminants such as pathogens, pollutants and synthetic materials discharged in sewer from homes and industries, which were often incinerated, dumped in occasion or land fill. As a result of rapidly increasing population, urbanization and industrialization, wastewater production and sewage sludge generation have increased manifold. Due to high cost of mineral fertilizers and escalating trends in their prices, there is an increasing trend of using sewage sludge in agriculture, especially under intensive cropping in arid and semi arid regions of the country. Therefore, application of sewage sludge to agricultural soils may be sustainable and economical due to nutrient cycling and disposal of sewage sludge. However, there may be a risk in use of sewage sludge due to potentially harmful contents present in the sludge such as heavy metals and pathogens. This paper, therefore, presents a review on various aspects of sewage sludge used in agriculture.

关键词： Sewage Sludge Sustainable Agriculture Pathogen Heavy Metals Soil Quality and Fertility

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Falkner-Skan aspects of a radiating(50%ethylene glycol D 50%water)-based hybrid nanofluid when Joule heating as well as Darcy-Forchheimer and Lorentz forces affect significantly

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Propulsion and Power Research 2023年第3期12卷 428-442页

作者： ghulam Rasool A.Wakif Xinhua Wang Ahmed Alshehri Abdulkafi Mohammed Saeed Institute of Intelligent Machinery Faculty of Materials and ManufacturingBeijing University of TechnologyBeijing 100124China Department of Mechanical Engineering Lebanese American UniversityBeirutLebanon Laboratory of Mechanics Faculty of Sciences Aïn ChockHassan II UniversityCasablancaMorocco Department of Mathematics Faculty of SciencesKing Abdulaziz UniversityJeddah 21589Saudi Arabia Department of Mathematics College of ScienceQassim UniversityBuraydah 51452Saudi Arabia

Falkner-Skan aspects are revealed numerically for a non-homogeneous hybrid mixture of 50%ethylene glycol-50%water,silver nanomaterials Ag,and molybdenum disul-fide nanoparticles MoS2 during its motion over a static wedge surface in a DarcyForchheimer porous medium by employing the modified Buongiorno *** Brownian and thermophoresis mechanisms are included implicitly along with the thermophysical properties of each phase via the mixture theory and some efficient phenomenological *** present simulation also accounts for the impacts of nonlinear radiative heat flux,magnetic forces,and Joule ***,the generalized differential quadrature method and Newton-Raphson technique are applied successfully for solving the resulting nonlinear boundary layer *** a limiting case,the obtained findings are validated accurately with the existing literature *** behaviors of velocity,temperature,and nanoparticles volume fraction are discussed comprehensively against various governing *** crucial results,it is revealed that the temperature is enhanced due to magnetic field,linear porosity,radiative heat flux,Brownian motion,thermophoresis,and Joule heating ***,it is depicted that the hybrid nanoliquids present a higher heat flux rate than the monotype nanoliquids and liquids ***,the surface frictional impact is minimized via the linear porosity ***,the surface heat transfer rate receives a prominent improvement due to the radiative heat flux inclusion.

关键词： Ethylene glycol-water hybrid fluid Hybrid nanofluid Falkner-Skan flow Nonlinear radiation Modified Buongiorno nanofluid model

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Ethnobotanical Studies on Dwarf Palm (<i>Nannorhops ritchieana</i>(Griff.) Aitchison) and Date Palm (<i>Phoenix dactylifera</i>L.) in Dera Ismail Khan, KPK, Pakistan

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American Journal of Plant Sciences 2012年第8期3卷 1162-1168页

作者： Sarfaraz Khan Marwat Khalid Usman Ejaz Ahmad Khan Said ghulam Jalaluddin Baloch Abdul Manan Tauqeer Fazal Ur Rehman Department of Economics Gomal University Dera Ismail Khan Pakistan Faculty of Agriculture Gomal University Dera Ismail Khan Pakistan Faculty of Pharmacy Gomal University Dera Ismail Khan Pakistan University WENSAM College Gomal University Dera Ismail Khan Pakistan

This paper is based on research work conducted during 2008-2010 in Dera Ismail Khan (D. I. Khan) District, Pakistan, to investigate Socio-Economic Impacts of Dwarf Palm (Nannorhops ritchieana) and Date Palm (Phoenix dactylifera) on the local inhabitants. A questionnaire for data collection of Dwarf Palm was designed and local people were interviewed. The main Dwarf Palm (Mazri) areas are lying between the Koh-i-Surkh and Marwat hill and yielding about 1000 tons of mazri leaves annually. Date Palm plantations are concentrated in Paniala, Paharpur, Chawdwan and Dhakki. The prominent varieties grown in the area are Dhakki, Shakri, Gulistan, Zaidi, Hillawi, Basra, Azadi and khudarawi. Dhakki Dates are well-known among all other varieties. Total area under dates cultivation in the district is 100,000 hectares, with total production of 11,000 tons dates. An average yield of Dhakki-Dates y-1 ranges from 6500 to 7000 tons i.e., 75% of the total production of dates in the area and dried dates production ranges from 800 - 1000 tons·year-1. A significant population is involved in processing of mazri and date palm leaves to prepare different products. Dates have a tremendous potential for economic uplift of the people. But growers face limitations and challenges regarding lack of information about customers and markets, harvesting, processing, and packaging techniques and infrastructure for producing good quality fresh dates. Fruit dropping in date palm particularly in Dhakki variety has become a serious concern. More than 70% date fruits drop before maturity. If production and processing constraints are properly handled, this may have significant socio-economic impact on local inhabitants.

关键词： Mazri Palm Date Palm Arecaceae Socio-Economic Impact

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鉴定和表达分析揭示棉花GH3第二亚家族成员参与纤维发育、激素响应和株型调控

鉴定和表达分析揭示棉花GH3第二亚家族成员参与纤维发育、激素响...

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中国农学会棉花分会2018年年会

作者：余道乾 ghulam Qanmber 鲁丽丽杨作仁李付广棉花生物学国家重点实验室/中国农业科学院棉花研究所

生长素诱导的基因调节植物生长和发育的许多方面。Gretchen Hagen 3 (GH3)基因家族是植物界广泛存在的3个生长素早期应答基因家族分支之一,参与吲哚-3-乙酸、水杨酸和茉莉酸等的动态平衡和逆境适应性调节过程;其中,GH3家族第二亚家族成... 详细信息

生长素诱导的基因调节植物生长和发育的许多方面。Gretchen Hagen 3 (GH3)基因家族是植物界广泛存在的3个生长素早期应答基因家族分支之一,参与吲哚-3-乙酸、水杨酸和茉莉酸等的动态平衡和逆境适应性调节过程;其中,GH3家族第二亚家族成员特异性地参与生长素的动态平衡调节,并涉及逆境响应过程。迄今为止,全基因组水平的棉花GH3家族基因的鉴定和分析工作尚未见报道。我们在亚洲棉(Gossypium arboreum)、雷蒙德氏棉(***)和陆地棉(***)中分别鉴定出10、10和20个GH3第二亚家族(SubfamilyⅡ)成员。生物信息学系统分析表明,20个GhGH3第二亚家族成员可被进一步划分为3个亚组(Subgroup 1-3),与植物中已鉴定分析的GH3基因相比,棉花GH3第二亚家族成员在基因结构、蛋白基序、酶活位点和数量比例上都很保守,表明它们在生长素动态平衡和应激反应中具有类似的作用。基于RNA测序数据和qRT-PCR (Quantitative real-time polymerase chain reaction)结果的表达模式分析显示20个GhGH3基因在根、茎、叶、花和发育中的胚珠和纤维的不同阶段中差异表达,表明其参与调节整个棉花植物的生长发育过程,并存在功能分化。我们进一步分析并汇总了GH3第二亚家族基因的启动子调控元件组成,并通过qRT-PCR的方法分析陆地棉中棉所24根和茎2个器官中GH3第二亚家族基因在生长素、水杨酸、赤霉酸和油菜素内酯处理后的响应特性。4种被测试的激素均以正负2种方式调节GH3基因的表达,且第一亚组(Subgroup 1)成员对几乎所有激素处理都表现出较高的敏感性。此外,我们测试了BR缺陷突变体pag1和相应野生型(WT)CCRI24中GhGH3基因的表达模式,差异表达结果证实了部分GH3B基因的BR响应特性,也暗示突变体pag1矮化或褶皱的表型部分是由于GH3家族基因的差异表达所引起的。而且,在拟南芥中异源超表达GhGH3.5基因的转基因株系表现出叶片变小且卷曲、植株矮化、叶柄变短等生长素缺陷的典型表型,表明其在株型方面的调控功能。总之,我们鉴定了棉花GH3第二亚家族基因,GhGH3在各种组织/器官/阶段中差异表达。对IAA、SA、BL和GA的响应特性以及pag1和CCRI24中的差异表达表明GhGH3基因的一些成员可能同时参与多种激素信号传导途径。由此,我们认为Gh GH3家族基因的某些成员可作为棉纤维改良、棉花株型塑造的候选基因。

关键词：陆地棉 GH3 植物激素表达模式顺式调控元件

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耐热高光泽水性聚氨酯/聚丙烯酸酯共聚树脂的合成研究

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中国印刷与包装研究 2014年第2期6卷 66-71页

作者： Aamer Saeed ghulam Shabir 王晓芳 Department of Chemistry Quaid-I-Azam UniversityIslamabad 45320Pakistan

0 引言水性聚氨酯（APU）因具有多种优异性能,如良好的附着力、柔韧性和耐冲击性,优良的耐磨性,与其他水性单体和树脂的良好相容性,而被广泛用作织物、塑料、木材、玻璃纤维和金属等基材的黏合剂或涂料.水性聚氨酯的合成是聚氨酯化学... 详细信息

0 引言水性聚氨酯（APU）因具有多种优异性能,如良好的附着力、柔韧性和耐冲击性,优良的耐磨性,与其他水性单体和树脂的良好相容性,而被广泛用作织物、塑料、木材、玻璃纤维和金属等基材的黏合剂或涂料.水性聚氨酯的合成是聚氨酯化学工业中发展最快的分支之一,研究人员对此做了大量的工作,但关于共聚材料的制备与性能研究鲜有报道,因此,相关研究成为此领域的研究重点.

关键词：聚氨酯水性丙烯酸酯合成树脂光泽化学工业研究人员

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