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Bluetongue Virus （BTV） Serological Survey and Evidence of Emergent BTV-8 Serotype in Morocco

Journal of Agricultural Science and Technology(A) 2014年第4期4卷 353-358页

作者： Kamar Drif Chafika Loutfi Ouafaa Fassi Fihri ghizlane sebbar Moulay Mustapha Ennaji MehdiElHarrak Department of Microbiology Immunology and Infectious Diseases Institute Hassan H of Agronomic and Veterinary Rabat 10101 Morocco Laboratory of Virology Society of Veterinarian Pharmaceutical and Biological Productions (BIOPHARMA) Rabat 10051 Morocco Laboratory of Virology Microbiology and Quality/Eeotoxieology and Biodiversity Faculty of Science and Techniques University Hassan H Mohammedia Casablanca Mohammedia 20800 Morocco

Bluetongue (BT) is an infectious, non-contagious arthropod-borne disease that infects all ruminants, including sheep, cattle, deer, goats and camelids. Bluetongue virus (BTV) belonged to Reoviridae is ARN genome of 19 Kb. Twenty-six BTV serotypes have long been recognized, to be associated with severe disease in certain breeds of sheep, whereas cattle and goats are usually sub-clinically affected. Before 2004, BT was considered an exotic disease in Morocco, however, the first outbreak was observed in 2004 in sheep. This outbreak was caused by the isolated BTV-4. Two years later a BTV-1 emerged in Morocco. Both serotype 1 and serotype 4 circulated after 2007 across the country. The aims of the present work was to perform a serological study on sheep from different regions in Morocco in order to clarify the current BTV epidemiological situation and its evolution from 2009 to 2012, to determine the co-infection rate, and to confirm the possible circulation of other BTV Serotype mainly the BTV-8. All of 436 sera were tested by serum neutralization using reference strains. Results confirm the presence of BTV-4, BTV-1 and BTV-8. However, the present study report for the first time the emerging BTV-8 circulation in Morocco. Moreover, the founding reveal as well a higher co-infection rate in cattle compared to sheep.

关键词：蓝舌病病毒血清学调查血清型 BTV 摩洛哥证据血清中和试验呼肠孤病毒

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Isolation and Identification of Mannheimia haemolytica and Pasteurella multocida Species from Ruminants in Six Different Regions in Morocco

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Journal of Agricultural Science and Technology(A) 2018年第6期8卷 398-405页

作者： ghizlane sebbar Khalil Zro Faouzi Kichou Abdelkrim Fillali Maltouf Bouchra Belkadi Society of Veterinary Pharmaceutical and Biological Productions(Biopharma) B.P.4569RabatMorocco Laboratory of Microbiology and Molecular Biology Faculty of SciencesUniversity Mohammed V4 Ibn Battouta Ave.BP 1014 RP RabatMorocco Institute of Agronomy and Veterinary Medicine Hassan II B.P.6202RabatMorocco

Pasteurella species is considered the principal pathogen of the respiratory *** haemolytica and Pasteurella multocida were investigated and typed from nasal swabs and tissues taken from sheep,goat and ***,41 lung and 121 nasal swabs samples were collected from animals with respiratory diseases during 2015 to 2017 in six different regions in *** first,a screening of Pasteurella species using the real time PCR(RT-PCR)was carried out,then all isolated strains on agar blood were confirmed by PCR gel based assay specific for *** and *** was evaluated in mice and histopathological examination was done on some of lung *** results revealed that 34 samples of which 28(55%)from nasal swabs and six(38%)from lungs were positive for *** and nine samples of which seven(14%)from nasal swabs and two(13%)from lungs were positive on *** serogroup ***-two percent(72%)isolates were highly pathogenic to mice,which is in accordance with the results obtained by histopathology *** is the first report for widespread infections of Pasteurella(***&***)in ruminants in ***,measures including development of vaccines are highly required to mitigate the impact of the bacteria in animals.

关键词： Isolation identification PCR Mannheimia haemolytica Pasteurella multocida histopathology Morocco.

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Diagnosis of Clinical Cases of Infectious Bursal Disease Using a Modified Rapid Taq Man-MGB Real-Time RT-PCR Assay

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Journal of Agricultural Science and Technology(A) 2018年第4期8卷 230-238页

作者： Maryame Cheggag Khalil Zro ghizlane sebbar Naoufal Rahmatallah Mohamed Mouahid Mohammed EL Houadfi Faouzi Kichou Unit of Histology and Pathological Anatomy Department of Veterinary Pathology and Public HealthInstitute of Agronomy and Veterinary Medicine Hassan IIB.P.6202RabatMorocco Society of Veterinary Pharmaceutical and Biological Productions(Biopharma) B.P.4569RabatMorocco Veterinary Clinic Temara Morocco

Infectious bursal disease(IBD)is an important contagious viral infection of immune system of *** infection possesses a permanent threat to the profitability of poultry industry *** aim of this work was to modify the Taq Man-MGB real-time reverse transcription-polymerase chain reaction(rRT-PCR)in one step involving two fluorogenic Taq Man labeled probe and using this protocol for detection of infectious bursal disease virus(IBDV)collected from suspected cases distributed in different regions of the country during the period *** intralaboratory validation of modified method was realized for specificity,linearity,repeatability,sensitivity and *** allowed reducing the test running time by six *** method was applied on 102 pools of bursa of fabricius(BF)samples collected from affected broiler farms suspected to be infected by *** showing macroscopic lesions including muscle petechial hemorrhages,hypertrophy and hemorrhage of BF,were subjected to molecular analysis using modified protocol“Taq Man-MGB rRT-PCR”.The validation satisfied all criteria and the assay developed could be a useful tool for a very rapid diagnosis of IBDV and permit to detect and to discriminate in one-step very virulent(vv)from non-vv(classic and variant)IBDV *** of 84 IBDV positive samples,a prevalence of 39%for vv strains and 61%for classical strains was *** results indicate that despite the vaccination against IBDV,the vv form of this pathologie continues to cause serious problems for Moroccan broiler *** obtained results indicate the successfully detection of IBDV and differentiated all vvIBDV strains from non-vvIBDV strains;Avian infectious agent RNA viruses tested are negative,demonstrating great specificity of the *** results obtained indicate that this method is suitable as a routine laboratory test for the rapid detection and differentiation of IBDV strains in samples of avian origin.

关键词： IBDV one-step rRT-PCR Taq Man-MGB macroscopic lesions

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