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Quantitative proteomic determination of diethylstilbestrol action on prostate cancer

Asian Journal of Andrology 2013年第3期15卷 413-420页

作者： Pierre Bigot Kevin Mouzat Muriel Bahut Nora Benhabiles geraldine cancel tassin Abdel-Rahmene Azzouzi Olivier Cussenot Department of Urology Angers University Hospital Angers 49933 France CeRePP Tenon Hospital Paris 75970 France Department of Biochemistry N'imes University Hospital NTmes 30029 France Plate-Forme Technologique de Biotechnologie Moleculaire Angers University Angers 49933 France 5CEA DSV IRCM SREIT Laboratoire de Cancerologie Experimentale Fontenay-aux-Roses 92265 France

Diethylstilbestrol （DES） has a direct cellular mechanism inhibition on prostate cancer. Its action is independent from the oestrogen receptors and is preserved after a first-line hormonal therapy. We aimed to identify proteins involved in the direct cellular inhibition effects of DES on prostate cancer. We used a clonogenic assay to establish the median lethal concentration of DES on 22RV1 cells. 22RV1 cells were exposed to standard and DES-enriched medium. After extraction, protein expression levels were obtained by two-dimensional differential in-gel electrophoresis （2D-DIGE） and isotope labelling tags for relative and absolute quantification （iTRAQ）. Proteins of interest were analysed by quantitative RT-PCR and western blotting. The differentially regulated proteins （P〈0.01） were interrogated against a global molecular network based on the ingenuity knowledge base. The 2D-DIGE analyses revealed DES-induced expression changes for 14 proteins （〉 1.3 fold; P〈0.05）. The iTRAQ analyses allowed the identification of 895 proteins. Among these proteins, 65 had a modified expression due to DES exposure （i.e., 23 overexpressed and 42 underexpressed）. Most of these proteins were implicated in apoptosis and redox processes and had a predicted mitochondrial expression. Additionally, ingenuity pathway analysis placed the OAT and HSBP1 genes at the centre of a highly significant network. RT-PCR confirmed the overexpression of OAT （P=0.006） and HSPB1 （P=0.046）.

关键词： cultured cells DES diethylstilbestrol ingenuity pathway analysis isotope labelling mass spectrometry prostate cancer proteomics

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8q24ll区与瓜德罗普岛（法属西印度群岛）的非洲裔加勒比男性前列腺癌进展的风险相关

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Asian Journal of Andrology 2015年第1期17卷 117-119,I0010页

作者： geraldine cancel-tassin Marc Romana Cecile Gaffory Pascal Blanchet Olivier Cussenot Luc Multigner CeRePP Pitie-Salpetriere Hospital Paris 75013 France ER2 UPMC Univ Paris 6 Paris 75020 France 3U665 Inserm Pointe a Pitre 97159 Guadeloupe French West Indies Department of Urology Assistance Publique Hopitaux de Paris Tenon Hospital GHU Est Paris 75020 France U1085 - IRSET Inserm Pointe Pitre 97154 Guadeloupe French West Indies Department of Urology CHU Pointe--Pitre/Abymes Pointe Pitre 97159 Guadeloupe French West Indies.

Multiple regions of the genome have been associated with the risk of prostate cancer in Caucasians, particularly including several polymorphisms located at 8q24. Region 2 of 8q24 has been repeatedly found to be associated with the risk of prostate cancer among men of African descent, although one study performed in the Caribbean island of Jamaica did not report this finding. In this study, the single nucleotide polymorphism rs16901979, located in region 2 of 8q24, was genotyped in 498 cases of histologically confirmed prostate cancer and 541 controls from the French Caribbean islands of Guadeloupe, where the population is largely of African descent. The AA genotype and the A allele at rs16901979 were associated with elevated risks of prostate cancer （odds ratios [ORs] = 1.84, 95% confidence interval [95% CI] = 1.26-2.69, P= 0.002 and OR = 1.36, 95% CI = 1.13-1.64, P= 0.001, respectively）. Following stratification of the patients by disease aggressiveness, as defined by the Gleason score, the pooled genotypes AC ＋ AA were associated with a higher risk of a Gleasen score 〉7 at diagnosis （OR = 1.79, 95% CI = 1.17-2.73, P= 0.007）. In summary, the A allele at rs16901979 was associated with the risk of prostate cancer in the Caribbean population of Guadeloupe, confirming its involvement in populations of African descent. Moreover, our study provides the first evidence of an association between this variant and the risk of aggressive prostate cancer.

关键词： African Continental Ancestry Group aggressiveness Caribbean region genetic predisposition to disease prostaticneoplasms

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The germline genetics of mild-to-moderate penetrance:An intriguing role of PRAME in multiple carcinogenesis

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Genes & Diseases 2024年第3期11卷 7-10页

作者： Diaddin Hamdan Van Tai Nguyen Justine Paris Christophe Leboeuf Morad El Bouchtaoui Marc Espie Anne Janin geraldine Falgarone Melanie Di Benedetto Guithem Bousquet Universite de Paris Cite INSERMUMR_S942MASCOTParis F-75006France Hopital La Porte Verte Versailles F-78004France National Cancer Hospital Cancer Research and Clinical Trials CenterHaNoi100000VietNam AP-HP-Hopital Saint Louis Centre de Maladies du SeinParisF-75010France Universite Sorbonne Paris Nord Bobigny F-93000France AP-HP-Hopital Avicenne Unite de Medecine AmbulatoireBobigny F-93000France

Germline genetics of high penetrance such as BRCA genes,mutations might be sufficient for carcinogenesis,but this only explains fewer than 10%of *** assume that most cancers are the result of germline mutations of low to moderate penetrance,combined with acquired mutations due to external carcinogenic *** their accumulation over time,aging is associated with an increased risk of multiple cancer development in a given *** decades,germline genetics have been based on familial linkage studies enabling most high-penetrance genes to be *** recently,population-wide studies have led to the identification of considerable numbers of polymorphisms associated with cancer ***,most of them are of unknown functional value,thus limiting their translational application.

关键词： carcinogenesis BRCA linkage

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新马泰全部免签！为何中国游客依旧纠结东南亚之旅

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环球人文地理 2024年第5期 50-59页

作者： Clear 黄元琪 anwar Thirawatana carloscastilla GuyChan billow926 geraldine Ng Kencana Studio

如今，东南亚黄金旅游路线“新马泰”的三个国家已全部对中国游客免签。然而宣布免签后，中国游客似乎依旧犹豫，客流量不如预期。人们还愿意去东南亚旅游吗？除了飞涨的物价，争议已久的安全问题，电信诈骗、大麻合法化……游客又该如...

如今，东南亚黄金旅游路线“新马泰”的三个国家已全部对中国游客免签。然而宣布免签后，中国游客似乎依旧犹豫，客流量不如预期。人们还愿意去东南亚旅游吗？除了飞涨的物价，争议已久的安全问题，电信诈骗、大麻合法化……游客又该如何处之？东南亚，尤其黄金旅游路线“新马泰”，曾经备受中国游客欢迎。

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国际护理知识网络建设的调查研究

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International Journal of Nursing Sciences 2024年第3期11卷 374-380,I0004,I0005页

作者： Margareth Santos Zanchetta Suzanne Fredericks Kateryna Metersky geraldine Martorella Pammla Petrucka Laurie Clune Kelly Graziani Giacchero Vedana Cristina Rosa Soares Lavareda Baixinho Cristianne Maria Famer Rocha Sara Campagna Sally Zhang He Márcia Teles de Oliveira Gouveia Marcelo Medeiros Denize Bouttelet Munari Daniel Gonzalo Eslava Albarracin Carlos Aguilera-Serrano Walterlania Silva Santos Toronto Metropolitan University TorontoCanada Florida State University TallahasseeUSA University of Saskatchewan ReginaCanada University of Regina ReginaCanada State of Sao Paulo University Ribeirao PretoBrazil Lisbon Superior School of Nursing LisbonPortugal Federal University of Rio Grande do Sul Porto AlegreBrazil University of Turin TurinItaly Scarborough Health Network ScarboroughCanada Federal University of Piauí TeresinaBrazil Federal University of Goias GoianiaBrazil Fundacion Universitaria Cafam BogotaColombia University of Malaga MalagaSpain University of Brasília Ceil^andiaBrazil

本文报告了为创建护理知识网络(Nursing Knowledge Network,NKN)对不同国家护理院校进行环境扫描调查所收集到的信息。采用在线方式(2021年2-10月)进行环境扫描,探索研究领域的确定、现有资源、预期收益、教学研究创新、知识传播障碍及... 详细信息

本文报告了为创建护理知识网络(Nursing Knowledge Network,NKN)对不同国家护理院校进行环境扫描调查所收集到的信息。采用在线方式(2021年2-10月)进行环境扫描,探索研究领域的确定、现有资源、预期收益、教学研究创新、知识传播障碍及网络的预期贡献。目标信息提供者包括来自13个国家63所大学的200名护理教师。要求每所大学有1名参与者回答问卷。共有42所大学(约70%)回复,包括巴西21所,加拿大4所,葡萄牙和西班牙各3所,哥伦比亚、墨西哥、秘鲁和美国各2所,智利、意大利和巴拉圭各1所。其分享了相关信息和见解:通过护理高等教育机构之间的协作和交流,全球可以加强共同创新和研究合作能力;较少被研究的领域是知识网络生成证据、为当地科学实践提供信息的潜在重点。这些信息将为进一步了解护理发展,以及地方、区域和全球护理证据应用和传播相关的政府政策与计划制订提供参考。

关键词：信息网络创新扩散国际教育交流知识管理护理教育护理教师实践护理院校跨文化护理

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Effective simulation of flexible lateral boundaries in two-and three-dimensional DEM simulations

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Particuology 2008年第6期6卷 483-500页

作者： geraldine Cheung Catherine O'Sullivan Department of Civil and Environmental Engineering Imperial College London

Discrete element method （DEM） models to simulate laboratory element tests play an important role in advancing our understanding of the mechanics of granular material response, including bonded or cemented, particulate materials. Comparisons of the macro-scale response observed in a real physical test and a ＂virtual＂ DEM-simulated test can calibrate or validate DEM models. The detailed, particle scale information provided in the DEM simulation can then be used to develop our understanding of the material behaviour. It is important to accurately model the physical test boundary conditions in these DEM simulations. This paper specifically considers triaxial tests as these tests are commonly used in soil mechanics. In a triaxial test, the test specimen of granular material is enclosed within a flexible latex membrane that allows the material to deform freely during testing, while maintaining a specified stress condition. Triaxial tests can only be realistically simulated in 3D DEM codes, however analogue, 2D, biaxial DEM simulations are also often considered as it is easier to visualize particle interactions in two dimensions. This paper describes algorithms to simulate the lateral boundary conditions imposed by the latex membrane used in physical triaxial tests in both 2D and 3D DEM simulations. The importance of carefully considering the lateral boundary conditions in DEM simulations is illustrated by considering a 2D biaxial test on a specimen of frictional unbonded disks and a 3D triaxial test on a bonded （cemented） specimen of spheres. The comparisons indicate that the lateral boundary conditions have a more significant influence on the local, particle-scale response in comparison with the overall macro-scale observations.

关键词： Triaxial testing Discrete element simulations Boundary conditions

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Regulation of neuronal survival by DNA methyltransferases

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Neural Regeneration Research 2017年第11期12卷 1768-1775页

作者： Judit Symmank geraldine Zimmer Institute of Human Genetics University Hospital JenaJenaGermany

The limited regenerative capacity of neuronal cells requires tight orchestration of cell death and survival regulation in the context of longevity, age-associated diseases as well as during the development of the nervous system. Subordinate to genetic networks epigenetic mechanisms like DNA methylation and histone modifications are involved in the regulation of neuronal development, function and aging. DNA methylation by DNA methyltransferases （DNMTs）, mostly correlated with gene silencing, is a dynamic and reversible process. In addition to their canonical actions performing cytosine methylation, DNMTs influence gene expression by interactions with histone modifying enzymes or complexes increasing the complexity of epigenetic transcriptional networks. DNMTs are expressed in neuronal progenitors, post-mi- totic as well as adult neurons. In this review, we discuss the role and mode of actions of DNMTs including downstream networks in the regulation of neuronal survival in the developing and aging nervous system and its relevance for associated disorders.

关键词： DNA methyltransferase I cortical interneurons PAK6 neuronal aging neuropsychiatric diseases neurodevelopment neuronal death

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Fine-tuning of cortical progenitor proliferation by thalamic afferents

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Neural Regeneration Research 2015年第6期10卷 887-888页

作者： Katrin Gerstmann geraldine Zimmer Institute of Human Genetics Jena University HospitalFriedrich-Schiller-University Jena CGphi MC UMR CNRS 5534 University Claude Bernard Lyon

During cerebral cortical cortex neurogenesis two major types of progenitors generate a variety of morphologically and functionally diverse projection neurons destined for the different cortical layers in non-gyrified mice. Radial glia cells （RGCs） undergo mitosis in the cortical ventricular zone and exhibit an apical-basal cell polarity, whereas non-polar intermediate progenitor cells （IPCs） divide basally in the subventricular zone （Franco and Muller, 2013; Taverna et al., 2014）.

关键词： Eph Fine-tuning of cortical progenitor proliferation by thalamic afferents

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DNMT1-dependent regulation of cortical interneuron function and survival

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Neural Regeneration Research 2021年第12期16卷 2405-2406页

作者： Daniel Pensold geraldine Zimmer-Bensch RWTH Aachen University Institute for Biology IIDepartment of Functional Epigenetics in the Animal ModelAachenGermany

Increased occurrence of age-associated disabilities and neurodegenerative diseases is the price we pay for the tremendous elevation in life expectancy in our modern *** comes along with structural,neurochemical and physiological alterations in the brain that cause memory decline and cognitive impairments(Rozycka and Liguz-Lecznar,2017).Numerous factors contribute to cognitive aging including hormonal,metabolic,and immune dysregulation,elevated oxidative stress and inflammation,changes in neurotransmission,and diminished neurotrophic support of neurons(Rozycka and Liguz-Lecznar,2017).

关键词： alterations elevated function

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LHX1–a multifunctional regulator in preoptic area-derived interneuron development

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Neural Regeneration Research 2019年第7期14卷 1213-1214页

作者： Judit Symmank geraldine Zimmer-Bensch Polyclinic for Orthodontics University Hospital Jena Department of Functional Epigenetics in the Animal Model Institute for Biology ⅡRWTH Aachen University

In the cerebral cortex,local inhibition is achieved by different types of gamma-aminobutyric(GABA)-expressing interneurons,representing essential key players of cortical information processing(Marin,2012).Their development has to be highly orchestrated to ensure proper numbers and subtype composition in the adult cortex and defects in interneuron development contribute to the pathophysiology of psychiatric diseases(Marin,2012).

关键词： cerebral cortex different types gamma-aminobutyric

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