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Glutamate dehydrogenase and Na^+-K^+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels

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Chinese Journal of Oceanology and Limnology 2011年第2期29卷 343-349页

作者：李二超 Leticia ARENA Gabriel LIZAMA Gabriela GAXIOLA gera.d cuzon Carlos ROSAS 陈立侨 Alain VAN WORMHOUdT School of Life Science East China Normal University UMR5178 Station de Biologie Marine du Musum National d'Histoire NaturelleBP225Concarneau 29900France Unidad Multidisciplinaria de docencia e Investigación Facultad de CienciasUniversidad Nacional Autónoma de MxicoPuerto de abrigo s/nSisal C.P.97350HunucmYucatnMexico IFREMER TahitiFrench Polynesia

Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp *** effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp(***) were *** involved an examination of growth performance,glutamate dehydrogenase(GdH) and Na+-K+ ATPase mRNA expression,,and GdH activity in muscles and *** experimental diets were formulated,containing 25%,40%,and 50% dietary protein,and fed to the shrimp at a salinity of *** 20 days,no significant difference was observed in weight gain,though GdH and Na+-K+ ATPase gene expression and GdH activity increased with higher dietary protein ***,shrimp fed diets with 25% and 50% dietary protein were transferred into tanks with salinities of 38 and 5,respectively,and sampled at weeks 1 and *** fed with 40% protein at 25 in salinity(optimal conditions) were used as a *** of the salinities,shrimp fed with 50% dietary protein had significantly higher growth performance than other diets;no significant differences were found in comparison with the *** fed with 25% dietary protein and maintained at salinities of 38 and 5 had significantly lower weight gain values after 2 *** salinity change also stimulated the hepatosomatic index,which increased in the first week and then recovered to a relatively normal level,as in the control,after 2 *** findings indicate that in white shrimp,the specific protein nutrient and energy demands related to ambient salinity change are associated with protein *** dietary protein level could improve the osmoregulation capacity of *** with more energy resources allocated to GdH activity and expression.

关键词：饲料蛋白水平谷氨酸脱氢酶盐度变化对虾养殖 ATP酶生长性能 Na 日粮蛋白质水平

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