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Expression of Toll-like Receptor 4 in Neonatal Cord Blood Mononuclear Cells in Patients with Preeclampsia

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2010年第5期30卷 615-619页

作者：夏革清徐丹芬吴敏吴超英 Department of Obstetrics and Gynecology Union HospitalTongji Medical CollegeHuazhong University of Science and Technology

The expression of Toll-like receptor 4 (TLR4) in neonatal cord blood mononuclear cells (MNCs) and serum TNF-α were investigated in order to explore the roles of TLR4 in the pathogenesis of *** study enrolled 27 patients suffering from preeclampsia (experimental group) and 21 normal pregnancy patients (control group).After MNCs were separated, the expression of TLR4 mRNA and protein was detected by using real-time quantitative PCR and Western blotting respectively, and the expression of TNF-α by using *** results showed the TLR4 mRNA level in cord blood MNCs (2-CT:0.07±0.17), TLR4 protein expression level (absorbance ratio:0.81%±0.15%) and TNF-α level (9.5±1.73 pg/mL) were all increased in experimental group as compared with control group with the differences being statistically significant (P<0.05).There was a positive correlation between the expression of TLR4 mRNA and TNF-α in both experimental group and control group (r=0.54 and 0.53, respectively, P<0.05).It was concluded that TLR4 expression in the experimental group of cord blood MNCs was increased and there was a positive correlation between the expression of TLR4 mRNA and TNF-α in both ***4-mediated release of inflammatory cytokines may be one of the important reasons leading to preeclampsia.

关键词： preeclampsia mononuclear cells Toll-like receptor 4 tumor necrosis factor α

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胎儿听力检测研究进展

中华耳科学杂志 2023年第4期21卷 591-595页

作者：史梦雯夏革清孙宇华中科技大学同济医学院附属协和医院耳鼻咽喉科武汉430022 华中科技大学同济医学院附属协和医院妇产科武汉430022

人类的耳部结构及听觉功能在胎儿时期就已发育完善,产前明确胎儿听觉功能可以达到指导生育或实现早期干预的目的。本文简要总结了胎儿期听觉系统发育及其评估方法,包括声刺激实验、脑磁图听觉诱发反应、影像学耳部畸形筛查、产前检测等... 详细信息

人类的耳部结构及听觉功能在胎儿时期就已发育完善,产前明确胎儿听觉功能可以达到指导生育或实现早期干预的目的。本文简要总结了胎儿期听觉系统发育及其评估方法,包括声刺激实验、脑磁图听觉诱发反应、影像学耳部畸形筛查、产前检测等,并初探胎儿镜在听力学领域的应用,以期助力精准防控耳聋出生缺陷。

关键词：先天性耳聋声刺激实验胎儿脑磁图胎儿镜

Preliminary suggestions on diagnosis and treatment of pregnancy complicated with COVID-19 in Wuhan Union Hospital

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中华妇产科杂志 2020年第2期 77-80页

作者： Zhu Ying Wu Qiulei Wang Lin Liu xiaoli Yuan Hongfang Wang Zheng Zhang Jinnong Zhao Lei Deng Feitao Jin Jing Zhang Yanling Tang Qianqian Chen Hui Zeng xiangzhi Wu Min Tian Yanhong xia geqing Department of Obstetrics and Gynecology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China Department of Laboratory Wuhan Red Cross Hospital Wuhan 430015 Department of Surgery Department of Emergency Department of Infection the first people''s Hospital of Tianmen City Tianmen 431700 China

By analyzing the clinical characteristics and diagnosis and treatment of COVID-19 in pregnancy, the preliminary suggestions on diagnosis and treatment of pregnancy complicated with COVID-19 in Wuhan Union Hospital were established. Five pregnant patients with COVID-19 were collected before January 26, 2020. One case was <28 pregnant weeks, the others for≥28 weeks; 3 cases were confirmed by nucleic acid test of novel coronavirus, and 2 cases were clinically diagnosed as suspected infection. The relevant clinical symptoms, auxiliary examination and treatment were summarized and analyzed. The main respiratory symptom was dry cough, the others were not typical in the early stage of pregnancy with COVID-19. The blood oxygen saturation decreased significantly within 72 hours after delivery and were significantly improved with symptomatic treatment. The blood routine examination showed that the lymphocyte count decreased and the eosinophil number decreased significantly, which was synchronized with the lymphocyte decline. Eosinophil count was 0 in typical clinical symptoms, and increased with the improvement of clinical symptoms. Severe cases and pregnant women on medium and late stage should be hospitalized and they should be admitted to ICU if necessary. Obstetric treatment should be used according to the specific conditions of mother and fetus. Pregnant patients with COVID-19 should be carefully distinguished during pregnancy due to its insidious, thus to avoid delay in early diagnosis and treatment. 72 hours after delivery is a critical period for close monitoring. The trend of eosinophil is declined significantly and correlated to the clinical manifestations, but the universality of this conclusion needs large sample study.

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一氧化氮与妊娠期胎儿窘迫相关性研究

同济医科大学学报 2000年第4期29卷 337-338页

作者：夏革清孙永玉同济医科大学附属协和医院妇产科武汉430022

为了探讨一氧化氮与妊娠期胎儿窘迫的关系 ,根据脐动脉血气分析结果选择 2 6例妊娠期胎儿窘迫的孕妇( 组 ) ,用 Griess法测母体血清及脐动脉血浆中亚硝酸盐浓度 ,并与正常胎儿的孕妇 ( 组 )比较。两组均为足月妊娠。结果发现 : 组母体... 详细信息

为了探讨一氧化氮与妊娠期胎儿窘迫的关系 ,根据脐动脉血气分析结果选择 2 6例妊娠期胎儿窘迫的孕妇( 组 ) ,用 Griess法测母体血清及脐动脉血浆中亚硝酸盐浓度 ,并与正常胎儿的孕妇 ( 组 )比较。两组均为足月妊娠。结果发现 : 组母体血及脐动脉血中 NO2 - 浓度分别为 (2 2 .75± 10 .48)、 (2 0 .39± 13.5 1)μmol/ L ,显著低于组[(74.82± 45 .36 )、 (6 1.42± 2 6 .0 7) μmol/ L],同时两组的母体血及胎儿脐动脉血 NO2 -浓度相关系数分别为 0 .812、0 .840。说明妊娠期胎儿窘迫时 NO水平较正常显著下降 ,提示 NO下降可能与慢性胎儿窘迫有关。

关键词：一氧化氮妊娠胎儿窘迫相关性研究

孕激素对重度子痫前期TLR4信号通路的调控作用

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实用妇产科杂志 2015年第11期31卷 828-831页

作者：吴敏曾想枝夏革清华中科技大学同济医学院附属协和医院湖北武汉430022

目的:研究孕激素在重度子痫前期孕妇外周血血浆中的水平及其在重度子痫前期发病机制Toll样受体4(TLR4)信号通路中的作用。方法:选择重度子痫前期孕妇30例(重度子痫前期组)和正常孕妇30例(正常对照组),采用稀释定量法分别测定其血浆孕激... 详细信息

目的:研究孕激素在重度子痫前期孕妇外周血血浆中的水平及其在重度子痫前期发病机制Toll样受体4(TLR4)信号通路中的作用。方法:选择重度子痫前期孕妇30例(重度子痫前期组)和正常孕妇30例(正常对照组),采用稀释定量法分别测定其血浆孕激素水平;原代培养重度子痫前期组孕妇外周血单个核细胞(PBMC),以不同浓度(0、10-8mol/L、10-6mol/L、10-4mol/L)孕激素(孕酮)刺激,实时定量聚合酶联反应检测TLR4、髓样分化因子88(My D88)、核转录因子-κB(NF-κB)mRNA的表达;酶联免疫吸附试验检测肿瘤坏死因子-α(TNF-α)及白介素-6(IL-6)蛋白的表达。结果:重度子痫前期组孕妇血浆孕激素水平明显低于正常对照组(P<0.05)。随着孕激素浓度的增加,重度子痫前期组孕妇TLR4、My D88、NF-κB mRNA的相对表达量逐渐减少,4组组间两两比较,差异均有统计学意义(P<0.01);TNF-α及IL-6蛋白的表达均减少,4组组间两两比较,差异均有统计学意义(P<0.01)。结论:孕激素水平在重度子痫前期孕妇外周血浆中明显降低,孕激素可在重度子痫前期发病机制TLR4信号通路中发挥抑制作用,从而对机体产生保护作用。

关键词：孕激素重度子痫前期 Toll样受体4 核转录因子-κB 肿瘤坏死因子-α

表皮生长因子抑制生殖道沙眼衣原体感染的实验研究

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华中科技大学学报（医学版） 2005年第6期34卷 770-772页

作者：夏革清陈红峰李红发华中科技大学同济医学院附属协和医院妇产科武汉430022 河南洛阳东方医院妇产科洛阳471003

目的探讨表皮生长因子(epidermal growth factor,EGF)对生殖道沙眼衣原体(Chlamydial trachoma-tis,CT)感染的影响。方法制备CT生殖道感染小鼠模型。CT阴道感染后,随机分成实验组(n=24)和对照组(n=24),分别用EGF及生理盐水干预,比较不... 详细信息

目的探讨表皮生长因子(epidermal growth factor,EGF)对生殖道沙眼衣原体(Chlamydial trachoma-tis,CT)感染的影响。方法制备CT生殖道感染小鼠模型。CT阴道感染后,随机分成实验组(n=24)和对照组(n=24),分别用EGF及生理盐水干预,比较不同时间各组CT感染率和阳性感染者阴道脱落菌量。结果CT感染动物模型制备成功;EGF干预组较对照组感染率明显下降;EGF组中阳性感染者阴道脱落菌量明显少于对照组。结论EGF能明显降低CT感染的发生率和降低CT的毒力,为预防生殖道CT感染开辟了新途径。

关键词：表皮生长因子沙眼衣原体生殖道感染

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妊娠并发重症肝炎妊娠结局临床分析

临床急诊杂志 2009年第2期10卷 93-94页

作者：夏革清吴超英华中科技大学同济医学院附属协和医院妇产科武汉430022

目的:分析妊娠并发重症肝炎不同妊娠结局的相关影响因素。方法:将1996年1月1日-2005年12月30日我院收治的妊娠并发重症肝炎患者8例,分析患者年龄、职业、孕周、病程、并发症、产科处理等因素与妊娠结局的关系。结果:8例患者,平均年龄26... 详细信息

目的:分析妊娠并发重症肝炎不同妊娠结局的相关影响因素。方法:将1996年1月1日-2005年12月30日我院收治的妊娠并发重症肝炎患者8例,分析患者年龄、职业、孕周、病程、并发症、产科处理等因素与妊娠结局的关系。结果:8例患者,平均年龄26岁,7例为农民,1例无固定职业。病程最长3个月,最短7d。产妇4例死亡,其中1例药物流产,3例自然临产分娩;4例存活,1例自然流产,1例早产,2例剖腹产。新生儿1例存活,1例足月死胎,3例早产死亡,3例流产。结论:患者职业、并发症、产科处理与妊娠结局明显相关,孕妇年龄及病程与妊娠结局无明显相关。

关键词：妊娠重症肝炎

荧光原位杂交产前诊断羊水间期细胞非整倍体的临床应用价值

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华中科技大学学报（医学版） 2009年第2期38卷 254-257页

作者：夏革清张丽萍谢宝国吴超英华中科技大学同济医学院附属协和医院妇产科武汉430022

目的探讨荧光原位杂交(fluorescenceinsituhybridization,FISH)诊断羊水间期细胞非整倍体的临床应用价值。方法收集547例孕妇中唐氏综合征筛查阳性者28例及3例具有其他产前诊断指征的孕妇,共计31例患者的羊水,应用13/21号染色体特异性... 详细信息

目的探讨荧光原位杂交(fluorescenceinsituhybridization,FISH)诊断羊水间期细胞非整倍体的临床应用价值。方法收集547例孕妇中唐氏综合征筛查阳性者28例及3例具有其他产前诊断指征的孕妇,共计31例患者的羊水,应用13/21号染色体特异性双色探针对羊水间期细胞进行FISH检测,以羊水培养细胞常规G显带核型分析作为FISH检测结果对照。结果31例标本中1例发生母血严重污染,其余30份标本均杂交成功(包括1例混浊羊水)。FISH检测结果发现典型21三体1例,未发现13三体。FISH诊断结果与核型分析结果一致。结论FISH产前诊断羊水间期细胞非整倍体准确率与核型分析一致,与核型分析相比,FISH具有快速、所需样本量少且结果直观易辨的优势,有较高的临床应用价值。

关键词：荧光原位杂交羊水间期细胞非整倍体产前诊断

Clinical presentations and outcomes of SARS-CoV-2 infected pneumonia in pregnant women and health status of their neonates

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Science Bulletin 2020年第18期65卷 1537-1542,M0003页

作者： Luming Xu Qianqian Yang Haojun Shi Shijun Lei xiaoli Liu Ying Zhu Qiulei Wu Xiuli Ding Yanhong Tian Qinghua Hu Fenghua Chen Zhi Geng xiangzhi Zeng Lin Lin Xuehong Cai Min Wu Zehua Wang Zheng Wang geqing xia Lin Wang Department of Clinical Laboratory Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Obstetrics and Gynecology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Gastrointestinal Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Respiratory Pathophysiology Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Radiology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Obstetrics and Gynecology Wuhan Red Cross HospitalWuhan 430015China Department of Obstetrics and Gynecology The First People's Hospital ofTianmenTianmen 431700China

The outbreak of COVID-19 has affected the world *** much remains unknown,the severity of the illness is quite *** disease outbreak is still ongoing with a rapidly growing number of patients.

关键词： infected Clinical pneumonia