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High-magnification observation of seminiferous tubules through the tunica albuginea by two-photon laser scanning microscopy

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Asian Journal of Andrology 2011年第5期13卷 774-776页

作者： Vincent Achard Pascal Weber georges mercier Marie-Pierre Blanchard Laboratoire d＇Histologie-Cytologie Faculte de Medecine secteur Timone Universite de la Mediterranee Marseille 13385 France Centre d＇Assistance Medicale a la Procreation AP-HM Conception Marseille 13385 France CNRS - IBDML - UMR6216 Service Imagerie - Case 907 Faculte des Sciences de Luminy Marseille 13288 France and Centre de Microscopie et Imagerie IFR Jean Roche Universite de la Mediterranee Marseille 13916 France

Testicular sperm extraction is widely used in the treatment of male infertility in cases of non-obstructive azoospermia. Identifying spermatogenetic foci within the testes is critical for testicular sperm extraction. Two-photon laser scanning microscopy （TPLSM） is an autofluorescence-based microscopy technique that allows observation at a cellular level in the depth of fresh living tissues and does not require any histological processing （fixation or staining）. The wavelengths previously used have shown no phototoxicity on sperm. We used TPLSM to detect spermatogenetic foci in fresh mouse testicular parenchyma without disrupting the tunica albuginea. Fresh surgically retrieved testes were observed using TPLSM within 1 h after extraction. Contralateral testes for each animal were observed using standard histology. Using TPLSM we were able to observe and measure the diameter of seminiferous tubules through the tunica albuginea, similar to the histological control. Structures within epithelial tubules were also observed, although their nature has yet to be identified. TPLSM is a real-time microscopy technique that could detect spermatogenetic foci.

关键词： seminiferous epithelium spermatogenesis

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A new hemostatic agent composed of Zn^(2+)-enriched Ca^(2+)alginate activates vascular endothelial cells in vitro and promotes tissue repair in vivo

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Bioactive Materials 2022年第12期7卷 368-382页

作者： Anne-Charlotte Ponsen Richard Proust Sabrina Soave Françoise mercier-Nom´e Isabelle Garcin Laurent Combettes Jean-Jacques Lataillade georges Uzan INSERM UMRS-MD 1197 Universit´e Paris-SaclayInstitut Andr´e LwoffHˆopital Paul BrousseVillejuifFrance Plateforme d’Histologie Immunopathologie de Clamart(PHIC) Universit´e Paris-SaclayINSERMCNRSInstitut Paris Saclay d’Innovation Th´erapeutiqueChˆatenay-MalabryFrance Universit´e Paris-Saclay INSERMInflammationMicrobiome and ImmunosurveillanceClamartFrance INSERM UMRS 1174 Universit´e Paris-SaclayOrsayFrance Centre de Transfusion Sanguine des Arm´ees ClamartFrance

To control capillary bleeding, surgeons may use absorbable hemostatic agents, such as Surgicel® and TachoSil®. Due to their slow resorption, their persistence in situ can have a negative impact on tissue repair in the resected organ. To avoid complications and obtain a hemostatic agent that promotes tissue repair, a zinc-supplemented calcium alginate compress was developed: HEMO-IONIC®. This compress is non-absorbable and is therefore removed once hemostasis has been achieved. After demonstrating the hemostatic efficacy and stability of the blood clot obtained with HEMO-IONIC, the impact of Surgicel, TachoSil, and HEMO-IONIC on cell activation and tissue repair were compared (i) in vitro on endothelial cells, which are essential to tissue repair, and (ii) in vivo in a mouse skin excision model. In vitro, only HEMO-IONIC maintained the phenotypic and functional properties of endothelial cells and induced their migration. In comparison, Surgicel was found to be highly cytotoxic, and TachoSil inhibited endothelial cell migration. In vivo, only HEMO-IONIC increased angiogenesis, the recruitment of cells essential to tissue repair (macrophages, fibroblasts, and epithelial cells), and accelerated maturation of the extracellular matrix. These results demonstrate that a zinc-supplemented calcium alginate, HEMO-IONIC, applied for 10 min at the end of surgery and then removed has a long-term positive effect on all phases of tissue repair.

关键词： Hemostatic agent Alginate Surgery Hemostasis Endothelial cells Tissue repair

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Role of acetic acid on U(Ⅵ) sorption on silica

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Nuclear Science and Techniques 2015年第1期26卷 127-134页

作者：赵羽佳韦悦周 Florence mercier-Bion Gregory Lefevre School of Nuclear Science and Engineering Shanghai Jiao Tong University Institut de Physique Nucleaire d’Orsay CNRS/IN2P3 Universite Paris-Sud 1115 rue Georges Clemenceau Orsay Cedex 91406 France Institut de Recherche de Chimie Paris CNRS-Chimie ParisTech 11 rue Pierre et Marie Curie Paris 75005 France

The influence of acetate on U(Ⅵ) sorption on silica from aqueous solutions was studied at p H 2–7 by complementary experimental methods of macroscopic measurement, spectroscopic investigation and thermodynamic calculation. Sorption percentage of U(Ⅵ) in the absence and in the presence of different acetate concentrations was determined by batch sorption procedures. Attenuated total reflection Fourier transform infra-red spectroscopy(ATR-FTIR) was used to elucidate the mechanisms of uranyl sorption on silica in the presence of acetate, by investigating, in-situ, the changes in the U(Ⅵ) sorption on silica surface in presence of ligand: the absorption bands of carboxylate ν(COO) in the range 1300–1700 cm-1and those around 850–950 cm-1of uranyl species νas(UO2) are followed. The decrease of U(Ⅵ) sorption with increasing acetate concentration was observed. Sorption of U(Ⅵ)-acetate(U-Ac) species on silica surface was demonstrated for the first ***-FTIR investigations clearly evidenced the absorption bands characteristic of sorbed U-Ac complexes both from the acetate and uranyl spectral regions those the wavenumbers correspond to sorbed species. No sequence effect of acetate and uranyl on the sorption on silica in ternary systems U(Ⅵ)-acetate-silica was observed from the sorption data. From the ATR-FTIR investigations, we can induce that the sorption of U-Ac leads to chemical equilibria and makes possible the modeling of sorption isotherms by surface complexation models. The related thermodynamic constants were modeled using CCM surface complexation modeling.

关键词：吸附作用傅里叶变换红外光谱硅胶 VI 醋酸 ATR-FTIR 表面络合模型 U(Ⅵ)

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