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Repellent effect of guava leaf volatiles on settlement of adults of citrus psylla, Diaphorina citri Kuwayama, on citrus

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Insect Science 2010年第1期17卷 39-45页

作者： Syed Muhammad Zaka Xin-Nian Zeng paul Holford george Andrew Charles Beattie Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education South China Agricultural University Guangzhou China Centre for Plant and Food Science University of Western Sydney Locked Bag 1797 Pennth South DC New South Wales 1797 Australia

The Asiatic citrus psyllid （Diaphorina cirri Kuwayama [Hemiptera： Sternorrhyncha： Psyllidae] is a vector ofhuanglongbing （citrus greening）, a devastating disease of citrus caused by phloem-limited bacteria. Growing guava （Psidium guajava） as an intercrop appears to be a successful means of reducing psyllid numbers within citrus orchards; however, the mechanism by which such a reduction is achieved is unknown. To determine the repellent effect of guava leaf and factors attributed to this activity, responses of adult psyllids to guava leaf and its odor were evaluated in cage tests and Y-tube olfactometer test. The results showed that guava leaf possessed a repellent effect against the adult citrus psyllids. Fewer psyllids were found on citrus leaves in the presence of guava foliage than in its absence. Young and old guava leaf showed equal repellent activity. By covering the guava shoots with net cloth, it was revealed that the repellent effect of guava leaf against adult psyllids on citrus was attributed to the volatile compounds, rather than physical factors. The olfactometer response of adult psyllids to guava leaf odor was dosage-dependent. Between guava odor and control, only 35.00%, 25.00% and 16.25% of the psyllids moved toward guava odor when presented with 5.0, 10.0 and 15.0 g of guava shoots, respectively. The olfactometer experiments also showed that both male and female psyllids responded similarly to the guava leaf odor.

关键词： Asiatic citrus psyllid huanglongbing (HLB) insect behavior Psidium guajava volatile compounds

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Anti-inflammatory effect of Heliotropium indicum Linn on lipopolysaccharide-induced uveitis in New Zealand white rabbits

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International Journal of Ophthalmology(English edition) 2016年第4期9卷 528-535页

作者： Samuel Kyei george Asumeng Koffuor paul Ramkissoon Elvis Ofori Ameyaw Emmanuel Akomanin Asiamah Discipline of Optometry School of Health Sciences College of Health Sciences University of Kwa Zulu-Natal Department of Optometry School of Allied Health SciencesUniversity of Cape Coast PMB Department of Pharmacology Faculty of Pharmacy and Pharmaceutical Sciences College of Health SciencesKwame Nkrumah University of Science and TechnologyPMB Department of Biomedical and Forensic Sciences School of Allied Health Science University of Cape Coast PMB Department of Pathology Komfo Anokye Teaching Hospital

AIM： To investigate the anti-inflammatory effect of an aqueous whole plant extract of Heliotropium indicum（HIE） on endotoxin-induced uveitis in New Zealand white rabbits.·METHODS： Clinical signs of uveitis including flares,iris hyperemia and miosis, were sought for and scored in1.0 mg/kg lipopolysaccharide（LPS）-induced uveitic rabbits treated orally with HIE（30-300 mg/kg）,prednisolone（30 mg/kg）, or normal saline（10 m L/kg）. The number of polymorphonuclear neutrophils infiltrating, the protein concentration, as well as levels of tumor necrosis factor-α（TNF-α）, prostaglandin E2（PGE2）, and monocyte chemmoattrant protein-1（MCP-1） in the aqueous humor after the various treatments were also determined. A histopathological study of the anterior uveal was performed.· RESULTS： The extract and prednisolone-treatment significantly reduced（P ≤0.001） both the clinical scores of inflammation（1.0-1.8 compared to 4.40 ±0.40 in the normal saline-treated rabbits） and inflammatory cells infiltration. The level of protein, and the concentrationsof TNF-α, PGE2 and MCP-1 in the aqueous humor were also significantly reduced（P ≤0.001）. Histopathological studies showed normal uveal morphology in the HIE and prednisolone-treated rabbits while normal saline-treated rabbits showed marked infiltration of inflammatory cells.· CONCLUSION： The HIE exhibits anti-inflammatory effect on LPS-induced uveitis possibly by reducing the production of pro-inflammatory mediators.

关键词： monocytechemotaticprotein-1 prostaglandin E2 tumor necrosis factor-α polymorphonuclear neutrophil iris hyperemia prednisolone heliotropium uveitis

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Controlled Environment Agriculture and Its Ability to Mitigate Food Insecurity

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Agricultural Sciences 2023年第2期14卷 298-315页

作者： Aubrey Lynn Garcia Mya Alexandria Catherine Griffith george paul.buss Xiusheng Yang John L. Griffis Sarah Bauer Ankit Kumar Singh Department of Ecology and Environmental Studies The Water School Florida Gulf Coast University Fort Myers FL USA Department of Marine and Earth Sciences The Water School Florida Gulf Coast University Fort Myers FL USA Department of Natural Resources and the Environment University of Connecticut Storrs CT USA Department of Environmental and Civil Engineering Mercer University Macon GA USA

The research objective of this review is to discuss the rationale that led to the development of Controlled Environment Agriculture (CEA) and investigate this agricultural approach as a potential solution to mitigate the increased pressures on food security. It describes the need for urban cultivation systems using controlled environments and how they can be harnessed to address pressures facing food security. The factors that have contributed to the growth of CEAs, education, environmental justice, and the advantages and disadvantages of growing crops in CEAs in urban areas will be discussed. The article reviews global urban cultivation systems using controlled environments, by identifying the technologies needed to establish them. The practice of CEA is being increasingly adopted worldwide and we describe urban agriculture and compare it with traditional growing systems. Indoor farming systems that integrate into existing urban infrastructure such as vertical farming and plant factories using CEAs are discussed. Indoor farming gives urban areas enhanced access to food sources, but the cost is high, however decreasing due to recent technological advances. The current review extends the literature by incorporating recent research on the topic of agriculture in urban areas and food security. This review seeks to provide additional information regarding the viability of CEA in urban areas.

关键词： Controlled Environment Agriculture Environmental Education Environmental Justice Food Deserts Urban Agriculture

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住院患者认知障碍识别和信息管理的整合性综述

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International Journal of Nursing Sciences 2024年第1期11卷 120-132页

作者：熊蓓蓓 Daniel X.Bailey paul Prudon Elaine M.Pascoe Leonard C.Gray Frederick Graham Amanda Henderson Melinda Martin-Khan Centre for Health Services Research Faculty of MedicineThe University of QueenslandBrisbaneAustralia Centre for Clinical Research Faculty of MedicineThe University of QueenslandRoyal Brisbane&Women’s HospitalBrisbaneAustralia Dementia and Delirium Division of MedicinePrincess Alexandra HospitalBrisbaneAustralia School of Nursing Faculty of HealthQueensland University of TechnologyBrisbaneAustralia Nursing Practice Development Unit Princess Alexandra HospitalBrisbaneAustralia School of Nursing Midwifery and Social SciencesCentral Queensland UniversityBrisbaneAustralia Griffith Health Griffith UniversityBrisbaneAustralia School of Nursing Midwifery and ParamedicineThe University of the Sunshine CoastBrisbaneAustralia Department of Health and Life Sciences University of ExeterExeterUnited Kingdom School of Nursing University of Northern British ColumbiaPrince GeorgeCanada

目的旨在对目前提供急性照护的医院识别认知障碍患者以及在病历中记录和管理认知相关信息的实际情况及政策要求和推荐方法进行综述。方法采用Whittemore和Knaf1的五步法,系统检索Medline,CINAHL和Scopus数据库,并检索了灰色文献来源。... 详细信息

目的旨在对目前提供急性照护的医院识别认知障碍患者以及在病历中记录和管理认知相关信息的实际情况及政策要求和推荐方法进行综述。方法采用Whittemore和Knaf1的五步法,系统检索Medline,CINAHL和Scopus数据库,并检索了灰色文献来源。纳人了提供急性照护的医院已实施的患者认知障碍识别和认知信息管理相关的文献。采用混合方法评价工具和灰色文献质量评估清单对文章质量进行评价。采用主题分析法呈现和整合结果。该综述已在PROSPERO预注册(CRD42022343577)。结果共22篇原始研究文献和10篇政府/行业文件被纳人分析。结果显示,实践和政策之间存在差距。尽管在政策层面上高度重视认知障碍的识别、认知相关信息的透明度,以及与患者、家属和照顾者(如适用)之间的互动,但有时在临床实践中,认知评估是非正式的,患者的认知相关信息未作记录,与患者、家属和照顾者的互动也存在不足。结论.通过整合认知评估,利用信息技术开发信息管理综合系统,建立相关法律法规,提供教育与和培训,并采用国家层面的策略,有可能显著改善对认知障碍患者的识别和护理。

关键词：急性照护认知功能障碍数据透明度谵妄痴呆健康信息管理与患者互动.

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The Comparative Performance of Plug Preparation Using Different Fertilizer Sources and Concentrations

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Agricultural Sciences 2023年第9期14卷 1193-1205页

作者： george paul.buss Paige Ann Carroll Mya Alexandria Catherine Griffith Xiusheng Yang John L. Griffis Galen Papkov Sarah Bauer Kathryn Jackson Ankit Kumar Singh Department of Marine and Earth Sciences The Water School Florida Gulf Coast University Fort Myers FL USA Department of Ecology and Environmental Studies The Water School Florida Gulf Coast University Fort Myers FL USA Department of Natural Resources University of Connecticut Storrs CT USA Department of Mathematics Florida Gulf Coast University Fort Myers FL USA Department of Environmental and Civil Engineering Mercer University Macon GA USA Independent Researcher Alexandria VA USA

Plugs are crucial for initiating crop production in greenhouses, soil, and controlled environment agriculture (CEA). Vegetable, fruiting, ornamental, and other horticultural crops that utilize plugs for production have demonstrated superior transplant establishment rate, plant health, and total yield. The APS Laboratory for Sustainable Food at Florida Gulf Coast University investigated the quality of plugs grown based on different concentrations and fertigation sources using synthetic and organic sources. We carried out the growth of “Rex Butterhead” Lettuce (Latuca sativa) plugs with five different fertigation treatments, 1) full-strength synthetic starter fertilizer solution;2) half-strength synthetic starter fertilizer solution;3) full-strength organic starter fertilizer solution;4) half-strength organic starter fertilizer solution, and 5) no fertilizer for control. Fertilizer treatments were formulated following manufacturer recommendations. The seeds were sown in Oasis® Horticubes and saturated every day with the different fertilizer treatments. The plugs were cultivated for 15 days in a controlled environment until two leaves after the cotyledons had developed. After 15 days, we collected data which included wet weight (g), dry weight (g), leaf area (cm2), and chlorophyll concentration (mg/cm2). In addition, we derived data including the Leaf Area Index (LAI, cm2/cm2) and Specific Leaf Area (SLA, cm2/g). Descriptive statistics were used to describe the biomass data. A Tukey’s HSD test was carried out to understand the differences between the fertilizer sources. We determined there was a statistically significant difference (P = 7.34E−29) in the measured plug growth parameters due to the various fertigation sources. We found that all fertilizer treatments produced viable plugs except for the control treatment. Of all the treatments, we concluded the half-strength organic treatment produced the mo

关键词： Controlled Environments Fertigation Lettuce Plugs Urban Agriculture

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Development of starch-based films reinforced with cellulosic nanocrystals and essential oil to extend the shelf life of red grapes

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Food Bioscience 2022年第3期47卷 403-412页

作者： Sneh Punia Bangar William Scott Whiteside Fatih Ozogul Kyle D.Dunno george Armstrong Cavender paul Dawson Department of Food Nutrition and Packaging SciencesClemson UniversitySC 29634USA Department of Seafood Processing Technology Faculty of FisheriesCukurova UniversityBalcaliAdana01330Turkey Department of Packaging Science Rochester Institute of TechnologyRochesterNYUSA

Packaging material has an important role in extending the shelf-life of *** study investigated the effectiveness of cellulose nanocrystals(CNCs)derived from Kudzu(Pueraria montana)vine to reinforce to starch-based *** addition,the essential oil was incorporated into these nanocomposite films to evaluate further improvement in film properties and *** packaging materials,including the pearl millet starch(PMS),PMS reinforced with Kudzu CNCs,and PMS reinforced with Kudzu CNCs stabilized pickering emulsion containing clove bud oil(CBO),was used to develop biodegradable nanocomposite *** films were investigated for their thermal,morphological,mechanical,and barrier *** addition,the films were used as packaging material to improve the shelf life of the grapes for 15 days at 5℃.PMS films reinforced with Kudzu CNCs stabilized pickering emulsion of CBO were most effective in extending the shelf life of red grapes up to 15 days at 5℃ by maintaining the weight,firmness,and soluble solids in grapes for a longer duration during *** study suggests that PMS+Kudzu CNCs+EO films have the potential for commercial packaging of fruits during short storage periods without adversely affecting their quality and sensory attributes.

关键词： Packaging material Nanocomposites Cellulose nanocrystals Red grapes Shelf life

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TYMS/KRAS/BRAF molecular profiling predicts survival following adjuvant chemotherapy in colorectal cancer

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World Journal of Gastrointestinal Oncology 2019年第7期11卷 551-566页

作者： Anastasios Ntavatzikos Aris Spathis paul Patapis Nikolaos Machairas Georgia Vourli george Peros Iordanis Papadopoulos Ioannis Panayiotides Anna Koumarianou Hematology-Oncology Unit 4th Department of Internal Medicine Medical School National and Kapodistrian University of Athens“ATTIKON” University Hospital Athens 12462 Greece Department of Cytopathology National and Kapodistrian University of Athens Medical School“ATTIKON” University Hospital Athens 12462 Greece 3rd Department of Surgery Medical School National and Kapodistrian University of Athens“ATTIKON” University Hospital Athens 12462 Greece Department of Hygiene Epidemiology and Medical Statistics Medical School National and Kapodistrian University of Athens Athens 11527 Greece Department of Surgery Medical School National and Kapodistrian University of Athens Evgenideio Therapeutirio S.A.“I AGIA TRIAS” Athens 11528 Greece 2nd Department of Pathology University of Athens Medical School“ATTIKON” University Hospital Athens 12462 Greece

BACKGROUND Patients with stage II-III colorectal cancer (CRC) treated with adjuvant chemotherapy, gain a 25% survival benefit. In the context of personalized medicine, there is a need to identify patients with CRC who may benefit from adjuvant chemotherapy. Molecular profiling could guide treatment decisions in these patients. Thymidylate synthase (TYMS) gene polymorphisms, KRAS and BRAF could be included in the molecular profile under consideration. AIM To investigate the association of TYMS gene polymorphisms, KRAS and BRAF mutations with survival of CRC patients treated with *** A retrospective study studied formalin-fixed paraffin-embedded tissues (FFPEs) of consecutive patients treated with adjuvant chemotherapy during January/2005-January/2007. FFPEs were analysed with PCR for the detection of TYMS polymorphisms, mutated KRAS (mKRAS) and BRAF (mBRAF). Patients were classified into three groups (high, medium and low risk) according to 5’UTR TYMS polymorphisms Similarly, based on 3’UTR polymorphism ins/loss of heterozygosity (LOH) patients were allocated into two groups (high and low risk of relapse, respectively). Cox regression models examined the associated 5- year survival outcomes. RESULTS One hundred and thirty patients with early stage CRC (stage I-II: 55 patients;stage III 75 patients;colon: 70 patients;rectal: 60 patients) were treated with surgery and chemotherapy. The 5-year disease free survival and overall survival rate was 61.6% and 73.9% respectively. 5’UTR polymorphisms of intermediate TYMS polymorphisms (2RG/3RG, 2RG/LOH, 3RC/LOH) were associated with lower risk for relapse [hazard ratio (HR) 0.320, P = 0.02 and HR 0.343, P = 0.013 respectively] and death (HR 0.368, P = 0.031 and HR 0.394, P = 0.029 respectively). The 3’UTR polymorphism ins/LOH was independently associated with increased risk for disease recurrence (P = 0.001) and death (P = 0.005). mBRAF (3.8% of patients) was associated with increased risk of death (HR 4.500,

关键词： Colorectal neoplasms Thymidylate synthase Untranslated regions Fluorouracil KRAS BRAF Prognosis

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The Comparative Performance of Soil-Based Systems with Hydroponics

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Agricultural Sciences 2023年第8期14卷 1087-1097页

作者： Paige Ann Carroll george paul.buss Mya Alexandria Catherine Griffith Xiusheng Yang John L. Griffis Jr. Galen Papkov Sarah Bauer Kathryn Jackson Ankit Kumar Singh Department of Ecology and Environmental Studies The Water School Florida Gulf Coast University Fort Myers FL USA Department of Marine and Earth Sciences The Water School Florida Gulf Coast University Fort Myers FL USA Department of Natural Resources University of Connecticut Storrs CT USA Department of Mathematics Florida Gulf Coast University Fort Myers FL USA Department of Environmental and Civil Engineering Mercer University Macon GA USA Independent Researcher Alexandria VA USA

Conventional soil-based agriculture is resource-intensive, utilizing large amounts of land and water, thereby placing a strain on Earth’s natural resources. Soil-based agricultural techniques create environmental issues such as soil degradation, deforestation, and groundwater pollution from the mass implementation of fertilizers and pesticides. Agricultural crop production using hydroponics has shown promise to be less resource intensive and provide a faster turnaround in crop production. Soilless cultivation using hydroponics promises to relieve some pressure on Earth’s ecosystems and resources by utilizing lesser land and water footprint. The APS Laboratory for Sustainable Food at Florida Gulf Coast University (FGCU) compared the growth of Lettuce Lactuca sativa “Rex Butterhead” crop grown using soil and soilless methods to analyze the growth performance in each setting. Crops grown in the soil-based medium were raised in the FGCU Food Forest, used a mix of soil and potting mix, watered regularly, and followed standard Integrated Pest Management (IPM) practices. Crops grown hydroponically were grown in a thermally insulated grow tent with an artificial lighting source, ventilation, environmental controls, and the Deep-Water Culture (DWC) method. Lettuce plugs were grown for 15 days in controlled environments until two leaves after the cotyledons had developed and were ready for transplant. Plugs were transplanted into a 4 × 6 matrix at the FGCU Food Forest and the DWC growth system. Crops were grown to full bloom and ready for harvest in the soil (60 days) and soilless (30 days) based setups. We collected crop growth data, including wet weight (g), dry weight (g), leaf area (cm2), and chlorophyll concentration (μmol/m2). From the collected data, we derived the Specific Leaf Area (SLA, cm2/g) and biomass productivity (kg/m2). Descriptive statistics were used to describe the collected and derived data. We investigated the

关键词： Controlled Environment Agriculture Hydroponics Lettuce Soilless Agriculture Urban Agriculture

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The Assessment of Growth Performance of Brassica rapa var. chinensis ‘Li Ren Choi’, Spinacia oleracea ‘Auroch’, Eruca sativa ‘Astro’, and Brassica rapa var. japonica Using GREENBOX Technology

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Agricultural Sciences 2023年第9期14卷 1222-1237页

作者： george paul.buss Paige Ann Carroll Mya Alexandria Catherine Griffith Xiusheng Yang John L. Griffis Jr. Galen Papkov Sarah Bauer Kathryn Jackson Ankit Kumar Singh Department of Marine and Earth Sciences The Water School Florida Gulf Coast University Fort Myers FL USA Department of Ecology and Environmental Studies The Water School Florida Gulf Coast University Fort Myers FL USA Department of Natural Resources University of Connecticut Storrs CT USA Department of Mathematics Florida Gulf Coast University Fort Myers FL USA Department of Environmental and Civil Engineering Mercer University Macon GA USA Independent Researcher Alexandria VA USA

Obtaining nutritious food is becoming increasingly difficult due to the growing urban population and the degradation of soil, water, and air from mechanized and industrialized agricultural techniques. More than half the global population resides in urban areas, with not enough surrounding agricultural land to meet food requirements. Food traveling long distances, an average of 1020 miles, has resulted in increased food miles for the average food item in the United States of America, representing wasted resources. The novel GREENBOX technology was invented in response to increasing pressures on food security. Previous studies conducted on GREENBOX technology assessed the technical feasibility of utilizing Lettuce Lactuca sativa ‘Rex Butterhead’. We at the APS Laboratory for Sustainable Food at Florida Gulf Coast University assessed the technical feasibility of growing different leafy green vegetable crops. GREENBOX technology consists of thermally insulated climate-controlled enclosures, an artificial lighting source, a soilless cultivation method (hydroponics), and environmental control modules. We assembled two GREENBOX units to assess the environmental conditions and growth performance of Brassica rapa var. chinensis ‘Li Ren Choi’, Spinach Spinacia oleracea ‘Auroch’, Arugula Eruca sativa ‘Astro’, and Mizuna Brassica Brassica rapa var. japonica. Plugs were cultivated and then transplanted in a randomized manner to the nutrient film technique (NFT) channels, subsequently grown for 30 days to full bloom and ready for harvest. Fertigation was carried out using a standard concentration nutrient solution. Crops were arranged in twelve blocks of four species each. We collected environmental data including daily light integral (DLI, mol/m2∙d), temperature (˚C), relative humidity (%), and vapor pressure deficit (VPD, kPa). Collected biomass data included wet weight (g), dry weight (g), leaf area (cm2), and chlorophyll concentration (mg/cm2)

关键词： Controlled Environment Agriculture Food Insecurity GREENBOX Hydroponics Lettuce

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The Comparative Performance of Nutrient-Film Technique and Deep-Water Culture Method of Hydroponics for GREENBOX Technology

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Agricultural Sciences 2023年第8期14卷 1108-1120页

作者： Mya Alexandria Catherine Griffith george paul.buss Paige Ann Carroll Xiusheng Yang John L. Griffis Jr. Galen Papkov Sarah Bauer Kathryn Jackson Ankit Kumar Singh Department of Ecology and Environmental Studies The Water School Florida Gulf Coast University Fort Myers FL USA Department of Marine and Earth Sciences The Water School Florida Gulf Coast University Fort Myers FL USA Department of Natural Resources University of Connecticut Storrs CT USA Department of Mathematics Florida Gulf Coast University Fort Myers FL USA Department of Environmental and Civil Engineering Mercer University Macon GA USA Independent Researcher Alexandria VA USA

With the rising pressures on food security, GREENBOX technology was developed as an avenue for fresh leafy vegetable crop production in urban settings. GREENBOX units were designed to be thermally insulated and climate controlled, with an artificial lighting source that utilized soilless cultivation techniques. Previous studies conducted on GREENBOX technology used the Nutrient Film Technique (NFT);however, various hydroponic methods exist, such as the Deep-Water Culture (DWC) method being the most used. The APS Laboratory for Sustainable Food at Florida Gulf Coast University (FGCU) compared the crop growth performance between DWC and NFT systems using GREENBOX technology. The following study monitored environmental conditions and compared productivity and biomass data of Rex Butterhead Lettuce crops between DWC and NFT systems. We assembled two GREENBOX units using commercially available materials and the standard nutrient solution for fertigation. The crops grown in DWC and NFT were in a 4 × 6 configuration. The DWC and NFT systems were used to grow Lettuce Lactuca sativa “Rex Butterhead” over 30 days to full bloom from prepared plugs grown for 14 days. We collected environmental data including Photosynthetic Photon Flux Density (PPFD, μmol/m2∙s), Daily Light Integral (DLI, mol/ m2∙d), temperature (˚C), relative humidity (%), and Vapor Pressure Deficit (VPD, kPa). We collected lettuce crop growth data, which included wet weight (g), dry weight (g), leaf area (cm2), and chlorophyll concentration (μmol/m2). We derived data, including the Specific Leaf Area (SLA, cm2/g) and biomass productivity (kg/m2), from previously collected data. We used descriptive statistics to present the collected data. A paired t-test was performed to understand the differences in biomass and productivity parameters between the DWC and NFT-grown lettuce crops. Both the DWC and NFT-grown crops could grow lettuce crops to harve

关键词： Controlled Environment Agriculture Food Insecurity GREENBOX Hydroponics Lettuce

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