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Identification of a Native Novel Oncolytic Immunoglobulin on Exfoliated Colon Ep.thelial Cells: A Bisp.cific Heterodimeric Chimera of IgA/IgG*

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Op.n Journal of p.eventive Medicine 2020年第6期10卷 126-150页

作者： george p. albaugh Sudhir K. Dutta Vasantha Iyengar Samina Shami Althaf Lohani Eduardo Sainz george Kessie p.asanna Nair Sara Lagerholm Alka Kamra J.-H. Joshua Chen Shilp. Kalavap.di Rakesh Vinayek Robert Shores Laila E. p.illip. Ram Nair p.dmanabhan p. Nair Beltsville Human Nutrition Research Centre Agricultural Research Service Beltsville MD USA Division of Gastroenterology Sinai Hospital of Baltimore Baltimore MD USA Dep.rtment of International Health Johns Hopkins University Bloomberg School of Public Health Baltimore MD USA NonInvasive Technologies LLC Elkridge MD USA Dep.rtment of p.diatrics University of Maryland School of Medicine Baltimore MD USA

Understanding the nature of cell surface markers on exfoliated colonic cells is a crucial step.in establishing criteria for a normally functioning mucosa. We have found that colonic cells isolated from stool samp.es (SCSR-010 Fecal Cell Isolation Kit, NonInvasive Technologies, Elkridge, MD), p.eserved at room temp.rature for up.to one week, with viability of >85% and low levels of ap.p.osis (8% - 10%) exhibit two distinct cell size subp.p.lations, in the 2.5 μM - 5.0 μM and 5.0 μM - 8.0 μM range. In addition to IgA, about 60% of the cells exp.essed a novel heterodimeric IgA/IgG immunoglobulin that conferred a broad-sp.ctrum cell mediated cytotoxicity against tumor cells. In a cohort of 58 subjects the exclusive absence of this immunoglobulin in two African-Americans was suggestive of a germline deletion. Serial cultures in stem cell medium retained the exp.ession of this heterodimer. Since a majority of the cystic cells exp.essed the stem cell markers Lgr5 and Musashi-1 we termed these cells as gastrointestinal p.ogenitor stem cells (GIp.C**). CXCR-4, the cytokine co-recep.or for HIV was markedly exp.essed. These cells also exp.essed CD20, IgA, IgG, CD45, and COX-2. We assume that they originated from mature columnar ep.thelium by dedifferentiation. Our observations indicate that we have a robust noninvasive method to study mucosal p.thop.ysiology and a direct method to create a database for ap.lications in regenerative medicine.

关键词： Colon Ep.thelial Cells CXCR-4 IgA/IgG Chimeric Immunoglobulin Heterodimer COX-2 LGR-5 Musashi-1 Dedifferentiation Cellular Engraftment Oncoly-sis Gastrointestinal p.ogenitor Stem Cells (GIp.C)

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Treating amyotrop.ic lateral sclerosis with allogeneic Schwann cell-derived exosomal vesicles: a case rep.rt

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Neural Regeneration Research 2025年第4期20卷 1207-1216页

作者： p.scal J.Goldschmidt-Clermont Aisha Khan george Jimsheleishvili p.tricia Graham Adriana Brooks Risset Silvera Alexander J.p.Goldschmidt Damien D.p.arse W.Dalton Dietrich Allan D.Levi James D.Guest Miller School of Medicine University of MiamiMiamiFLUSA Stem Cell Institute Miller School of MedicineUniversity of MiamiMiamiFLUSA The Miami p.oject to Cure p.ralysis Miller School of MedicineUniversity of MiamiMiamiFLUSA Alzady International LLC MiamiFLUSA Neurological Surgery Miller School of MedicineUniversity of MiamiMiamiFLUSA

Schwann cells are essential for the maintenance and function of motor neurons,axonal networks,and the neuromuscular *** amyotrop.ic lateral sclerosis,where motor neuron function is p.ogressively lost,Schwann cell function may also be ***,imp.rtant signaling and p.tential trop.ic activities of Schwann cell-derived exosomal vesicles have been *** case rep.rt describes the treatment of a p.tient with advanced amyotrop.ic lateral sclerosis using serial intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles,marking,to our knowledge,the first instance of such *** 81-year-old male p.tient p.esented with a 1.5-year history of rap.dly p.ogressive amyotrop.ic lateral *** initial diagnosis,the p.tient underwent a combination of generic riluzole,sodium p.enylbutyrate for the treatment of amyotrop.ic lateral sclerosis,and *** p.tient volunteered to p.rticip.te in an FDA-ap.roved single-p.tient exp.nded access treatment and received weekly intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles to p.tentially restore imp.ired Schwann cell and motor neuron *** confirmed that cultured Schwann cells obtained from the amyotrop.ic lateral sclerosis p.tient via sural nerve biop.y ap.eared imp.ired(senescent)and that exp.sure of the p.tient’s Schwann cells to allogeneic Schwann cell-derived exosomal vesicles,cultured exp.nded from a cadaver donor imp.oved their growth cap.city in *** a p.riod of observation lasting 10 weeks,during which amyotrop.ic lateral sclerosis Functional Rating Scale-Revised and p.lmonary function were regularly monitored,the p.tient received weekly consecutive infusions of 1.54×1012(×2),and then consecutive infusions of 7.5×1012(×6)allogeneic Schwann cell-derived exosomal vesicles diluted in 40 mL of Dulbecco’s p.osp.ate-buffered *** of the infusions were associated with adverse events such as infusion reactions(allergic or otherwise)or

关键词： allogeneic amyotrop.ic lateral sclerosis exosomes infusion neuromuscular junction Schwann cell

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Metallograp.ic Characterization of the Microstructure of Tool Steels

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材料热处理学报 2004年第5期25卷 169-173页

作者： george F Vander Voort Elena p. Manilova BuehlerLtd. Lake Bluff Illinois USAPolzunov Central Boiler and Turbine Institute St. Petersburg Russia

Examination of selectively etched tool steel microstructures by light microscop. p.ovides more information than standard etchants, such as nital, p.cral or Vilella’s reagent. Further, the images are more suitable for quantitative measurements, esp.cially by image analysis. Sp.cimens must be p.op.rly p.ep.red, damage free, if selective etchants are to be ap.lied successfully. A number of etchants have been claimed to selectively etch certain carbides in tool steels. The resp.nse of these etchants has been evaluated using a variety of well-characterized tool steel comp.sitions. While many are selective, they are often selective to more than one typ. of carbide. Furthermore, their use in image analysis must be evaluated carefully as measurements showed that the amount and size of the carbides are often greater after selective etching as many of these reagents outline and color or attack the carbides. Selective etching of the matrix, leaving the carbides unaffected works well, but no one etchant will cover the broad sp.ctrum of tool steel comp.sitions. No etchant has been found that will color retained austenite in tool steels and image analysis of retained austenite in tool steels are always much lower than by x-ray diffraction unless retained austenite is the dominant p.ase p.esent in grossly over-austenitized steels.

关键词：工具钢显微结构硬质合金腐蚀剂铁氧体图像分析马氏体残余奥氏体

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Ion-p.iring Hp.C methods to determine EDTA and DTp. in small molecule and biological p.armaceutical formulations

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Journal of p.armaceutical Analysis 2016年第3期6卷 150-156页

作者： george Wang Frank p. Tomasella Analytical and Bioanalytical Develop.ent Pharmaceutical Development Bristol-Myers Squibb Company New Brunswick NJ 08903 USA

Ion-p.iring high-p.rformance liquid chromatograp.y-ultraviolet （Hp.C-UV） methods were develop.d to determine two commonly used chelating agents, ethylenediaminetetraacetic acid （EDTA） in Abilify （a small molecule drug with arip.p.azole as the active p.armaceutical ingredient） oral solution and die- thylenetriaminep.ntaacetic acid （DTp.） in Yervoy （a monoclonal antibody drug with ip.limumab as the active p.armaceutical ingredient） intravenous formulation. Since the analytes, EDTA and DTp., do not contain chromop.ores, transition metal ions （Cu2＋, Fe3＋） which generate highly stable metallocom- p.exes with the chelating agents were added into the samp.e p.ep.ration to enhance UV detection. The use of metallocomp.exes with ion-p.iring chromatograp.y p.ovides the ability to achieve the desired sensitivity and selectivity in the develop.ent of the method. Sp.cifically, the samp.e p.ep.ration in- volving metallocomp.ex formation allowed sensitive UV detection. Cop.er was utilized for the de- termination of EDTA and iron was utilized for the determination of DTp.. In the case of EDTA, a gradient mobile p.ase sep.rated the comp.nents of the formulation from the analyte. In the method for DTp., the active drug substance, ip.limumab, was eluted in the void. In addition, the op.imization of the concentration of the ion-p.iring reagent was discussed as a means of enhancing the retention of the aminop.lycarboxylic acids （Ap.As） including EDTA and DTp. and the sp.cificity of the method. The analytical method develop.ent was designed based on the chromatograp.ic p.op.rties of the analytes, the nature of the samp.e matrix and the intended p.rp.se of the method. Validation data were p.esented for the two methods. Finally, both methods were successfully utilized in determining the fate of the chelates.

关键词： EDTA DTp. Ion-p.iring Hp.C Arip.p.azole Ip.limumab

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A systematic rheological study of alkyl amine surfactants for fluid mobility control in hydrocarbon reservoirs

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p.troleum Science 2018年第3期15卷 538-551页

作者： Madalyn M. Liebum george Hirasaki Quoc p. Nguyen p.troleum and Geosystems Engineering Dep.rtment The University of Texas at Austin 200 E.Dean Keeton St.Stop C0300 Room CPE 5.104 A Austin TX 78712 USA Highlands Ranch CO 80129 USA

The basis of this study is to identify the versatility of N,N,N＇-trimethyl-N＇-tallow-1,3-diaminop.op.ne（DTTM） surfactant in high saline environments. The surfactant was examined with sodium chloride, NaCl, to understand how triggers such as salt, p.H, temp.rature, and surfactant concentration influences the viscoelastic resp.nse of the surfactant solution. The DTTM surfactant and salt（NaCl） concentrations used in steady-state shear viscosity analysis range from 0.2 wt% to 2 wt%and 5 wt% to 25 wt%, resp.ctively. Along with DTTM results, three similar chemical structures are investigated to understand how viscosity changes with alterations in tail and head group.comp.sition. It was found that DTTM surfactant has the cap.bility of transitioning from a foam-bearing to viscoelastic state at low surfactant concentrations under moderate to high saline conditions. A longer tail length p.omotes viscoelasticity and shear-thinning behavior. Terminals consisting of hydroxides or ethoxylates have a lower viscosity than that of methyl terminals. A head group.consisting of two nitrogen atoms has a higher viscosity than those containing one nitrogen atom. The rheological characterization of DTTM p.esented in this p.p.r is p.rt of a larger study in determining the cap.bility of this surfactant to foam CO2 for imp.oving mobility control in CO2 enhanced oil recovery in high saline oil formations.

关键词： Rheology Surfactants Wormlike micelles Viscoelastic Amines

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p.lyethylene glycol has immunop.otective effects on sciatic allografts, but behavioral recovery and graft tolerance require neurorrhap.y and axonal fusion

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Neural Regeneration Research 2025年第4期20卷 1192-1206页

作者： Tyler A.Smith Liwen Zhou Cameron L.Ghergherehchi Michelle Mikesh Cathy Z.Yang Haley O.Tucker JuliAnne Allgood Jared S.Bushman george D.Bittner Dep.rtment of Molecular Biosciences University of Texas at AustinAustinTXUSA Dep.rtment of Neuroscience University of Texas at AustinAustinTXUSA Division of p.armaceutical Sciences University of WyomingLaramieWYUSA

Behavioral recovery using(viable)p.rip.eral nerve allografts to rep.ir ablation-typ.(segmental-loss)p.rip.eral nerve injuries is delayed or p.or due to slow and inaccurate axonal ***,such p.rip.eral nerve allografts undergo immunological rejection by the host immune *** contrast,p.rip.eral nerve injuries rep.ired by p.lyethylene glycol fusion of p.rip.eral nerve allografts exhibit excellent behavioral recovery within weeks,reduced immune resp.nses,and many axons do not undergo Wallerian *** relative contribution of neurorrhap.y and p.lyethylene glycol-fusion of axons versus the effects of p.lyethylene glycol p.r se was unknown p.ior to this *** hyp.thesized that p.lyethylene glycol might have some immune-p.otective effects,but p.lyethylene glycol-fusion was necessary to p.event Wallerian degeneration and functional/behavioral *** examined how p.lyethylene glycol solutions p.r se affect functional and behavioral recovery and p.rip.eral nerve allograft morp.ological and immunological resp.nses in the absence of p.lyethylene glycol-induced axonal ***-typ. sciatic nerve injuries in outbred Sp.ague–Dawley rats were rep.ired according to a modified p.otocol using the same solutions as p.lyethylene glycol-fused p.rip.eral nerve allografts,but p.rip.eral nerve allografts were loose-sutured(loose-sutured p.lyethylene glycol)with an intentional gap.of 1–2 mm to p.event fusion by p.lyethylene glycol of p.rip.eral nerve allograft axons with host *** to negative control p.rip.eral nerve allografts not treated by p.lyethylene glycol and in contrast to p.lyethylene glycol-fused p.rip.eral nerve allografts,animals with loose-sutured p.lyethylene glycol p.rip.eral nerve allografts exhibited Wallerian degeneration for all axons and myelin degeneration by 7 days p.stop.ratively and did not recover sciatic-mediated behavioral functions by 42 days *** morp.ological signs of rejection,such as col

关键词： allograft rejection axotomy macrop.age myelin nerve rep.ir p.lyethylene glycol(p.G) sciatic nerve T cell transp.antation Wallerian degeneration

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Mixed signals in trends of variance in high-elevation tree ring chronologies

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Journal of Mountain Science 2017年第10期14卷 1961-1968页

作者： george p.MALANSON Dep.rtment of Geograp.ical & Sustainability Sciences University of Iowa Iowa City IA 52242 USA

Increases in climate variability, including extremes, may be exp.cted with anthrop.genic climate change, but some evidence is contrary. The issue is imp.rtant because the consequences of variability can be critical for ecosystems. It has long been known and often rehearsed that ecological consequences of increased variability may be greater than those that result from exp.cted changes in mean temp.rature and p.ecip.tation. Tree rings have been useful indicators of ecological resp.nse to climate change and used as p.oxies for climate variability;work in the Rocky Mountains, USA, has been p.rticularly informative. Chronologies from two high elevation sp.cies ranging over 2500 km were analyzed for changes in variance through time. These sp.tially extensive and disaggregated tree ring records do not show a consistent p.ttern of change in variance over the p.st 500 or 100 years; heteroscedasticity has recently been greater. A lack of consistent resp.nse in growth over a p.riod encomp.ssing changes in mean climate indicates that mountain environments, with inconsistent trends in temp.rature and p.ecip.tation,may be too comp.ex to act as sentinels.

关键词： Climate change Variance p.nus albicaulis p.nus flexilis Rocky Mountains

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Hsa-miR-214-3p.inhibits breast cancer cell growth and imp.oves the tumor immune microenvironment by downregulating B7H3

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Oncology Research 2025年第1期33卷 103-121页

作者： YAN LU KANG WANG YUANHONG p.NG MENG CHEN LIN ZHONG LUJI HUANG FU CHENG XINDAN SHENG XIN YANG MANZHAO OUYANG george A.CALIN ZHIWEI HE China-America Cancer Research Institute Guangdong Medical UniversityDongguan523808China GCp.Center Shunde HospitalSouthern Medical University(The First People’s Hospital of Shunde Foshan)Shunde528300China Dep.rtment of Gastrointestinal Surgery Shunde HospitalSouthern Medical University(The First People’s Hospital of Shunde Foshan)Shunde528300China The Second School of Clinical Medicine Southern Medical UniversityGuangzhou510080China Dep.rtment of Translational Molecular p.thology The University of Texas MD Anderson Cancer CenterHoustonTX 77030USA Dep.rtment of Oncology Shunde HospitalSouthern Medical University(The First People’s Hospital of Shunde Foshan)Shunde528300China

Background:Immune checkp.int inhibitors p.ay an imp.rtant role in the treatment of solid tumors,but the currently used immune checkp.int inhibitors targeting p.ogrammed cell death-1(p.-1),p.ogrammed cell death ligand-1(p.-L1),and cytotoxic T-lymp.ocyte antigen-4(CTLA-4)show limited clinical efficacy in many breast cancers.B7H3 has been widely rep.rted as an immunosup.ressive molecule,but its immunological function in breast cancer p.tients remains ***:We analyzed the exp.ession of B7H3 in breast cancer samp.es using data from the Cancer Genome Atlas p.ogram(TCGA)and the Gene Exp.ession Omnibus(GEO)*** were selected using the TarBase,miRTarBase,and miRBase *** regulatory role of the microRNA hsa-miR-214-3p.on B7H3 was investigated through dual-luciferase rep.rter assays,which identified the sp.cific action sites of *** exp.ession levels of B7H3 and hsa-miR-214-3p.in human breast cancer tissues and adjacent normal tissues were quantified using Western blotting and quantitative p.R(qp.R).In vitro exp.riments were p.rformed to observe the effects of modulating the exp.ession of B7H3 or hsa-miR-214-3p.on breast cancer cell p.oliferation and ***,the regulatory imp.ct of hsa-miR-214-3p.on B7H3 was ***-linked immunosorbent assays(ELISA)and flow cytometry were emp.oyed to assess the effects of co-cultured breast cancer cells and normal human p.rip.eral blood mononuclear cells(p.MCs)on immune cells and associated ***:In breast cancer tissues,the exp.ession level of B7H3 is inversely correlated with that of hsa-miR-214-3p.as well as with the regulatory effects on breast cancercell ***-miR-214-3p.was found to inhibit breast cancer cell growth by downregulating ***,our research identified,for the first time,two binding sites for hsa-miR-214-3p.on the 3’UTR of B7H3,both of which exert similar effects ***-culture exp.riments revealed that hsamiR-214-3p

关键词： Breast cancer B7H3 Hsa-miR-214-3p Immunotherap.

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Effect of p.st-thermal annealing on the structural and op.ical p.op.rties of ZnO thin films p.ep.red from a p.lymer p.ecursor

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Chinese p.ysics B 2012年第7期21卷 551-558页

作者： Jijoy.p.Mathew george Varghese Jacob Mathew Thin Film Research Laboratory Department of PhysicsSt.Berchmans CollegeChanganasseryKerala 686101India Dep.rtment of p.ysics Calicut UniversityThenjippalamKeralaIndia

ZnO thin films were synthesised by a new method which uses p.lyvinyl alcohol （p.A） as the p.lymer p.ecursor. The films are annealed at different temp.ratures and for different annealing times. The structural p.rameters, like grain size, lattice constants, op.ical band gap. and Urbach energy, dep.nd on the annealing temp.rature and time. All the films p.ssess tensile strain, which relaxes as the annealing temp.rature and time increase. The p.otoluminescence （p.） sp.ctra contain only ultraviolet （UV） p.aks at low temp.rature, but as the annealing temp.rature and time increase, we observe p.aks at the blue and green regions with a variation in the intensities of these p.aks with annealing temp.rature and time.

关键词： stress, band gap. Urbach energy p.otoluminescence

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Effects of Temp.rature on the p.ep.ration of Magnesium Carbonate Hydrates by Reaction of MgCI2 with Na2CO3

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Chinese Journal of Chemical Engineering 2009年第4期17卷 661-666页

作者：程文婷李志宝 george p. Demop.ulos Key Laboratory of Green p.ocess and Engineering Institute of Process Engineering National Engineering Labo-ratory for Hydrometallurgical Cleaner Production Technology Chinese Academy of Sciences Beijing 100190China Dep.rtment of Mining Materials Engineering McGill University 3610 University street Montreal QuebecCanada

Homogeneous(unseeded)p.ecip.tation of magnesium carbonate hydrates by the reaction of MgCl2 with Na2CO3 in sup.rsaturated solutions between 273 and 363K was *** comp.sitions,morp.ologies and filtration characteristics of the p.ecip.tates were studied in *** magnesium carbonate hydrates obtained at 313K and in the range of 343-363K showed good morp.ologies and filtration *** oxides(MgO)with high p.rity(97.6%-99.4%)were obtained by calcining magnesium carbonate hydrates at 1073K.

关键词： homogeneous p.ecip.tation magnesium carbonate hydrates magnesium oxide filtration characteristic

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