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genes & Diseases 2024年第3期11卷 148-150页

作者： chen Tan Lingwa Wang Yifan Yang Shizhi He george g.chen Jason YK.Chan Michael CF.Tong C.A.van Hasselt Wenbin Xu Ling Feng Ru Wang Jugao Fang Department of Otolaryngology Head and Neck Surgery Beijing Tongren HospitalCapital Medical UniversityBeijing 100730China Key Laboratory of Otolaryngology Head and Neck Surgery(Ministry of Education of China) Beijing Institute of OtolaryngologyBeijing 100730China Department of Otorhinolaryngology Head and Neck SurgeryThe Chinese University of Hong KongPrince of Wales HospitalHongKong SAR999077China Department of Medical genetics Institute of Basic Medical ScienceChinese Academy of Medical Science&Peking Union Medical CollageBeijing 100730China

Locoregionally advanced laryngeal and hypopharyngeal cancers(LA-LHCs)are traditionally treated with surgery followed by postoperative radiotherapy,which impairs speech and swallowing functions and reduces the quality of life.^(1,2)The use of induction chemotherapy(IC)as a larynxpreserving approach for LA-LHCs has been verified and refined.^(3)However,the short-term tumor response to IC varies,non-responders usually show poor survival and little benefit.

关键词： chemotherapy laryngeal surgery

半边旗中二萜类化合物5F葡糖糖苷的分离鉴定及其抗肿瘤作用

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中成药 2010年第2期32卷 257-260页

作者：龚先玲陈志红梁念慈邓亦峰 george g.chen 广东医学院天然药物研究与开发重点实验室广东湛江524023 广东医学院分析中心广东湛江524023 香港中文大学外科系

目的:进一步研究半边旗的抗肿瘤活性成分。方法:运用反复硅胶柱层析的方法对半边旗中的化学成分进行分离纯化,以LC-MS及各种有机波谱法鉴定化学单体结构;并通过MTT法对分离鉴定的单体化合物及其苷元进行抗肿瘤活性研究。结果:从中分离鉴... 详细信息

目的:进一步研究半边旗的抗肿瘤活性成分。方法:运用反复硅胶柱层析的方法对半边旗中的化学成分进行分离纯化,以LC-MS及各种有机波谱法鉴定化学单体结构;并通过MTT法对分离鉴定的单体化合物及其苷元进行抗肿瘤活性研究。结果:从中分离鉴定1个二萜苷类化合物,为11β-hydroxy-15-oxo-ent-kaur-16-en-19-oic acid 19-β-D-glucoside(Ⅰ);化合物Ⅰ及其苷元5F均可抑制两种肿瘤细胞A549和CNE-2Z的生长,且它们对两种肿瘤细胞的抑制作用呈一定剂量依赖关系和时间依赖关系,化合物5F的活性强于化合物Ⅰ。结论:α,β不饱和环戊酮结构是其活性部位,5F中19位碳上的羧基与糖连接形成酯苷,成为化合物Ⅰ后,其抗肿瘤活性大大降低。

关键词：半边旗抗肿瘤成分二帖化合物成分分析

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半边旗抗肿瘤有效成分5F的纯化工艺研究

中成药 2008年第9期30卷 1286-1289页

作者：龚先玲陈志红吕应年邓亦峰 george g.chen 梁念慈广东医学院天然药物研究与开发重点实验室广东湛江524023 广东医学院分析中心广东湛江524023 香港中文大学外科系癌症中心

目的:探索D101大孔吸附树脂制备半边旗中抗肿瘤有效成分贝壳杉烷类二萜5F的工艺。方法:以HPLC为检测手段,以5F在浸膏中的含量为指标,比较甲醇和氯仿提取5F的专属性;以HPLC和TLC为检测手段,考察D101大孔吸附树脂对5F的吸附和洗脱条件。结... 详细信息

目的:探索D101大孔吸附树脂制备半边旗中抗肿瘤有效成分贝壳杉烷类二萜5F的工艺。方法:以HPLC为检测手段,以5F在浸膏中的含量为指标,比较甲醇和氯仿提取5F的专属性;以HPLC和TLC为检测手段,考察D101大孔吸附树脂对5F的吸附和洗脱条件。结果:半边旗药粉采用氯仿提取,制成含乙醇20%的药液上柱后,先用2BV30%的乙醇除去极性杂质,再用4BV60%的乙醇洗脱,得到质量分数为32.25%的5F。结论:建立了一种5F的纯化工艺,并达到要求的标准。

关键词：半边旗 5F D101大孔吸附树脂 HPLC

半边旗提取物5F-Na盐溶液对三种肝细胞癌细胞株的增殖抑制作用

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辽宁中医杂志 2010年第9期37卷 1814-1815页

作者：吴科锋刘义吕应年 george g.chen 梁念慈广东天然药物研究与开发实验室广东湛江524023 香港中文大学外科学系广东医学院生物化学与分子生物学研究所研究所广东湛江524023

目的:评估半边旗提取物5F-Na盐溶液对肝细胞癌细胞株的增殖抑制作用。方法:采用MTT法,观察不同浓度的半边旗提取物物5F-Na盐溶液分别作24、48、72h对三种不同类型肝细胞癌细胞株(Hepg2,Hep3B,BEL-7402)生长增殖的影响。结果:不同浓度的... 详细信息

目的:评估半边旗提取物5F-Na盐溶液对肝细胞癌细胞株的增殖抑制作用。方法:采用MTT法,观察不同浓度的半边旗提取物物5F-Na盐溶液分别作24、48、72h对三种不同类型肝细胞癌细胞株(Hepg2,Hep3B,BEL-7402)生长增殖的影响。结果:不同浓度的半边旗提取物5F-Na盐溶液分别对3种肝癌细胞作用24、48、72h后,所有的肝癌细胞株生长增殖均受到不同程度的抑制(Pg2,Hep3B,BEL-7402作用72h后的IC50分别是20.67,16.74,16.09mg/L。结论:半边旗提取物5F-Na盐溶液能有效抑制肝细胞癌细胞株的体外增殖。

关键词：半边旗提取物5F-Na盐溶液肝细胞癌

半边旗提取物5F-Na盐溶液的制备及体外抑癌作用研究

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中国药房 2010年第47期21卷 4419-4421页

作者：吴科锋吕应年刘义李立 george g.chen 赖宝山梁念慈广东天然药物研究与开发实验室湛江市524023 香港中文大学外科学系香港特别行政区999077 广东医学院生物化学和分子生物学研究所湛江市524023

目的:制备5F-Na盐,并观察其对肺癌细胞株NCL-H460和肝癌细胞株BEL-7402的杀伤作用。方法:加入0.5mol·L-1NaOH使半边旗提取物5F完全溶解,稀HCl调节pH值为7.4,加入磷酸盐缓冲液使其pH值稳定,高效液相色谱法测定5F-Na盐溶液中5F的含量。通... 详细信息

目的:制备5F-Na盐,并观察其对肺癌细胞株NCL-H460和肝癌细胞株BEL-7402的杀伤作用。方法:加入0.5mol·L-1NaOH使半边旗提取物5F完全溶解,稀HCl调节pH值为7.4,加入磷酸盐缓冲液使其pH值稳定,高效液相色谱法测定5F-Na盐溶液中5F的含量。通过MTT法检测所制备5F-Na盐溶液对肺癌细胞株NCL-H460和肝癌细胞株BEL-7402的杀伤作用。结果:所制备5F-Na盐溶液中5F为标示量的(100.51±2.18)%,其对NCl-H460细胞株24、48、72h的IC50分别为23.7、14.2、7.0μg·mL-1,对BEL-7402细胞株24、48、72h的IC50分别为157.3、41.5、21.0μg·mL-1。结论:本研究制备的5F-Na盐溶液能有效抑制癌细胞株生长增殖。

关键词：半边旗 5F-Na盐溶液制备抗肿瘤

半边旗提取物5F对肝细胞癌BEL-7402生长抑制作用及增殖细胞核抗原表达的影响

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中国现代医学杂志 2010年第24期20卷 3687-3690页

作者：吴科锋刘义吕应年 george g.chen 梁念慈广东天然药物研究与开发实验室广东湛江524023 香港中文大学外科学系广东医学院生物化学与分子生物学研究所广东湛江524023

目的研究半边旗提取物5F对肝细胞癌BEL-7402细胞生长的抑制作用。方法 MTT法检测5F对BEL-7402细胞的生长抑制作用;用Hoechst33258荧光染色法检测细胞凋亡;流式细胞仪检测5F对细胞周期的影响;免疫细胞化学法检测5F对增殖细胞核抗原(PCNA... 详细信息

目的研究半边旗提取物5F对肝细胞癌BEL-7402细胞生长的抑制作用。方法 MTT法检测5F对BEL-7402细胞的生长抑制作用;用Hoechst33258荧光染色法检测细胞凋亡;流式细胞仪检测5F对细胞周期的影响;免疫细胞化学法检测5F对增殖细胞核抗原(PCNA)表达的影响。结果 5F抑制BEL-7402细胞的生长,其效果与5F的浓度和作用时间相关,24、48和72h的IC50分别为60.33、21.32和11.22 mg/L;荧光双染色法显示经5F作用后细胞出现变形,染色质浓缩,产生凋亡小体;细胞被阻滞在g2/M,PCNA阳性表达率降低。结论 5F在体外能有效地抑制BEL-7402细胞的生长,其诱导凋亡作用可能与下调增殖细胞核抗原(PCNA)表达有关。

关键词：半边旗提取物5F BEL-7402 生长抑制增殖细胞核抗原

半边旗提取物5F对不同类型NSCLC细胞的增殖抑制作用

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山东医药 2009年第52期49卷 6-8页

作者：吴科锋刘义吕应年 george g.chen 梁念慈广东天然药物研究与开发实验室广东湛江524023 香港中文大学广东医学院

目的观察半边旗提取物5F(Psl5F)对不同类型非小细胞肺癌(NSCLC)细胞株的增殖抑制作用,并找出对其敏感的细胞株类型。方法采用MTT法观察不同浓度Psl5F分别在作用24、48、72 h时对不同类型的NSCLC细胞株(A549、SPCA-I、NCI-H460、PgCL3)... 详细信息

目的观察半边旗提取物5F(Psl5F)对不同类型非小细胞肺癌(NSCLC)细胞株的增殖抑制作用,并找出对其敏感的细胞株类型。方法采用MTT法观察不同浓度Psl5F分别在作用24、48、72 h时对不同类型的NSCLC细胞株(A549、SPCA-I、NCI-H460、PgCL3)生长增殖的影响。结果不同浓度的Psl5F对4种肺癌细胞作用24、48、72 h后,所有NSCLC细胞株生长增殖均受到不同程度的抑制,其中以对NCI-H460肺癌细胞的杀伤作用最强(PgCL3作用72 h后的半数致死量(IC50)分别是12.12、10.74、3.91、4.94 mg/L。结论Psl5F能有效抑制NSCLC细胞株的体外增殖,且对大细胞肺癌NCI-H460最为敏感。

关键词：半边旗提取物5F 癌,非小细胞肺半数致死量

PES1 promotes the occurrence and development of papillary thyroid cancer by upregulating the ERα/ERβ protein ratio

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PES1 promotes the occurrence and development of papillary th...

Chondroitinase ABC combined with Schwann cell transplantation enhances restoration of neural connection and functional recovery following acute and chronic spinal cord injury

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Yi-Bo Qiu Ling-Yao Liao Rong Jiang Man Xu Lin-Wan Xu george g.chen Zhi-Min Liu Department of Biochemistry and Molecular Biology Chongqing Medical University Department of Pathology Molecular Medicine and Cancer Research CenterChongqing Medical University Department of Surgery Chinese University of Hong KongPrince of Wales Hospital

Estrogen has been suggested to play a role in the occurrence and development of papillary thyroid cancer(PTC).The balance and the ratio between ERα and ERβ protein are crucial in determining the effect of estrogen on target tissues and ***1,a BRCT domain-containing protein,has been shown to play a role in modulating the balance and the ratio between ERα and ERβ protein,which is associated with the occurrence and development of breast and ovarian ***,the role of PES1 in connection with the balance and the ratio between ERα and ERβ protein in the occurrence and development of PTC remains *** this study,PES1 protein level in human PTC tissues and cells was examined using immunohistochemistry and Western blotting,*** correlations of PES1 protein level with ERα and ERβ protein levels,with various clinicopathological features of PTC patients,with the ERα/ERβ protein ratio and the proliferation,invasion and migration of PTC cells were assessed using statistical *** results showed that ERα and ERβ were co-expressed in human PTC tissues and ***α promoted and ERβ inhibited the proliferation,invasion and migration of PTC cells and a Yin-Yang balance existed between ERα and ERβ.PES1 modulated this Yin-Yang balance by elevating ERα and reducing ERβ protein level,then upregulating the ERα/ERβ protein ratio and promoting the proliferation,invasion and migration of PTC *** PTC tissues,PES1 protein level positively correlated with ERα(rs = 0.549,P gatively correlated with ERβ protein level(rs =-0.353,P gradually increased and ERβ protein level was decreased by degree in the occurrence and development of *** PES1 and ERα and decreased ERβ protein levels correlated with aggressive PTC behaviors such as large tumor size,extrathyroidal extension(ETE),lymph node metastasis(LNM) and high TNM *** was suggested that PES1 promotes the occurrence and development of P

关键词： PES1 ERα ERβ papillary thyroid cancer

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Neural Regeneration Research 2025年第5期20卷 1467-1482页

作者： Wenrui Qu Xiangbing Wu Wei Wu Ying Wang Yan Sun Lingxiao Deng Melissa Walker chen chen Heqiao Dai Qi Han Ying Ding Yongzhi Xia george Smith Rui Li Nai-Kui Liu Xiao-Ming Xu Spinal Cord and Brain Injury Research group Stark Neurosciences Research InstituteDepartment of Neurological SurgeryIndiana University School of MedicineIndianapolisINUSA Department of Hand Surgery the Second Hospital of Jilin UniversityChangchunJilin ProvinceChina Shriners Hospitals Pediatric Research Center Temple University School of MedicinePhiladelphiaPAUSA Department of Anatomy&Cell Biology Indiana University School of MedicineIndianapolisINUSA

Schwann cell transplantation is considered one of the most promising cell-based therapy to repair injured spinal cord due to its unique growth-promoting and myelin-forming properties.A the Food and Drug Administration-approved Phase I clinical trial has been conducted to evaluate the safety of transplanted human autologous Schwann cells to treat patients with spinal cord injury.A major challenge for Schwann cell transplantation is that grafted Schwann cells are confined within the lesion cavity,and they do not migrate into the host environment due to the inhibitory barrier formed by injury-induced glial scar,thus limiting axonal reentry into the host spinal *** we introduce a combinatorial strategy by suppressing the inhibitory extracellular environment with injection of lentivirus-mediated transfection of chondroitinase ABC gene at the rostral and caudal borders of the lesion site and simultaneously leveraging the repair capacity of transplanted Schwann cells in adult rats following a mid-thoracic contusive spinal cord *** report that when the glial scar was degraded by chondroitinase ABC at the rostral and caudal lesion borders,Schwann cells migrated for considerable distances in both rostral and caudal *** Schwann cell migration led to enhanced axonal regrowth,including the serotonergic and dopaminergic axons originating from supraspinal regions,and promoted recovery of locomotor and urinary bladder ***,the Schwann cell survival and axonal regrowth persisted up to 6 months after the injury,even when treatment was delayed for 3 months to mimic chronic spinal cord *** findings collectively show promising evidence for a combinatorial strategy with chondroitinase ABC and Schwann cells in promoting remodeling and recovery of function following spinal cord injury.

关键词： axonal regrowth bladder function chondroitinase ABC functional recovery glial scar lentivirus migration Schwann cell spinal cord injury transplantation

Interactions of molten salts with cathode products in the FFC Cambridge Process

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International Journal of Minerals,Metallurgy and Materials 2020年第12期27卷 1572-1587页

作者： george Z.chen Department of Chemical and Environmental Engineering Faculty of EngineeringUniversity of NottinghamNottingham NG72RDUK Department of Chemical and Environmental Engineering Faculty of Science and EngineeringUniversity of Nottingham Ningbo ChinaNingbo 315100China

Molten salts play multiple important roles in the electrolysis of solid metal compounds,particularly oxides and sulfides,for the extraction of metals or *** of these roles are positive in assisting the extraction of metals,such as dissolving the oxide or sulfide anions,and transporting them to the anode for discharging,and offering the high temperature to lower the kinetic barrier to break the metal-oxygen or metal-sulfur ***,molten salts also have unfavorable effects,including electronic conductivity and significant capability of dissolving oxygen and carbon dioxide *** addition,although molten salts are relatively simple in terms of composition,physical properties,and decomposition reactions at inert electrodes,in comparison with aqueous electrolytes,the high temperatures of molten salts may promote unwanted electrode-electrolyte *** article reviews briefly and selectively the research and development of the F ray-F arthing.chen(FFC)Cambridge Process in the past two decades,focusing on observations,understanding,and solutions of various interactions between molten salts and cathodes at different reduction states,including perovskitization,non-wetting of molten salts on pure metals,carbon contamination of products,formation of oxychlorides and calcium intermetallic compounds,and oxygen transfer from the air to the cathode product mediated by oxide anions in the molten salt.

关键词： FFC Cambridge Process molten salts electrolysis extraction oxides sulfides metals alloys reaction mechanisms