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Small Ubiquitin-Like Modifier Conjugating Enzyme with Active Site Mutation Acts as Dominant Negative Inhibitor of SUMO Conjugation in Arabidopsis

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Journal of Integrative Plant Biology 2013年第1期55卷 75-82页

作者： Konstantin Tomanov Christian Hardtke Ruchika Budhiraja Rebecca Hermkes george coupland Andreas Bachmair Department of Biochemistry and Cell Biology Max F. Perutz Laboratories Center for Molecular Biology University of Vienna Department of Plant Molecular Biology University of Lausanne Department of Plant Developmental Biology Max Planck Institute for Plant Breeding Research

Small ubiquitin-like modifier （SUMO） conjugation affects a broad range of processes in plants, including growth, flower initiation, pathogen defense, and responses to abiotic stress. Here, we investigate in vivo and in vitro a SUMO conjugating enzyme with a Cys to Ser change in the active site, and show that it has a dominant negative effect. In planta expression significantly perturbs normal development, leading to growth retardation, early flowering and gene expression changes. We suggest that the mutant protein can serve as a probe to investigate sumoylation, also in plants for which poor genetic infrastructure precludes analysis via loss-of-function mutants.

关键词： Agroinfection conjugating enzyme dominant-negative active site mutation small ubiquitin-like modifier conjugation small ubiquitin-like modifier.

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SUMO PROTEASE RELATED TO FERTILITY 1 and -2,the SUMO proteases,are required for fertility in Arabidopsis

SUMO PROTEASE RELATED TO FERTILITY 1 and -2,the SUMO proteas...

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从植物科学到农业发展——2012全国植物生物学大会

作者： george coupland Max Planck Institute for Plant Breeding Carl-von-Linne Weg 10D-50829 CologneGermany

Sumoylation is involved in extensive cellular processes in *** planta,SUMO is implicated in flowering-time control,responses to biotic and abiotic ***,we report that two proteins,SUMO PROTEASE RELATED TO FERTILITY(SPF) 1 and 2,regulate a number of aspects of reproductive fertility in Arabidopsis *** single spfl mutant exhibits some abnormalities in flower structure and embryos,but the pollen and ovules are morphologically normal,while the single spfl mutant is largely wild type. However,spfl enhances the phenotype of spf1 mutants so that the spfl spfl double mutant exhibits more serious abnormalities in micro- and mega-gametophytes and *** data of both RT-PCR and promoter GUS fusions shows that expression of these two genes is largely confined to reproductive ***1 and SPF2 can interact with different SUMO isoforms in ***1 protein displays in vitro a SUMO protease activity,and the pattern of SUMO-conjugate accumulation in the inflorescences,but not in seedlings,of the spf1 spf2 mutant differs from that of the wild *** results indicate that SPF1 and SPF2 are SUMO proteases active in reproductive tissues of Arabidopsis and contribute to fertility.

关键词： SUMO fertility gametophytes embryogenesis Arabidopsis

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SUMO PROTEASE RELATED TO FERTILITY 1 and-2,the SUMO proteases,are required for fertility in Arabidopsis

SUMO PROTEASE RELATED TO FERTILITY 1 and-2,the SUMO protease...

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2013全国植物生物学大会

作者： LIU Linpo JIANG Ying ZHANG Xiaomei WANG Xu HAN Yuzhen george coupland FU Yongfu MOA Key Lab of Soybean Biology(Beijing) Institute of Crop SciencesThe National Key Facility for Crop Gene Resources and Genetic ImprovementChinese Academy of Agricultural Sciences College of Biological Sciences State Key Laboratory of Plant Physiology and BiochemistryChina Agricultural University Max Planck Institute for Plant Breeding

Sumoylation is involved in extensive cellular processes in *** planta,SUMO is implicated in flowering-time control,responses to biotic and abiotic ***,we report that two proteins,SUM

关键词： SUMO SUMO protease fertility microgametophyte megagametophyte embryo

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