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Alterations in phosphoproteome under salt stress in Thellungiella roots

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Chinese Science Bulletin 2010年第32期55卷 3673-3679页

作者： ZHOU YiJun GAO Fei LI XiaoFeng zhang Jun zhang genfa Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education College of Life Science Beijing Normal University Beijing 100875 China College of Life and Environmental Sciences Minzu University of China Beijing 100081 China Departments of Anesthesiology Neurosurgery Biomedical Sciences Texas Tech University Health Science Center El Paso TX 79905 USA

High salinity stress is a major environmental factor that limits plant's distribution and productivity. An Arabidopsis-related halophyte,Thellungiella halophila,is an emerging model system used for plant abiotic stress tolerance research. Previous studies have suggested that protein phosphorylation has a crucial role in the high salinity response in plants. However,the phosphoproteome differential expression under high salinity stress in halophytes has not been well studied. In this report,phosphoproteome differential expression was analyzed under high salinity stress in Thellungiella roots. Twenty-six putative phosphoproteins were found to have changed expression pattern at the post-translational level. Twenty of these were identified by mass spectrometric analysis,including 18 upregulated and two downregulated phosphoproteins. These proteins were involved in a variety of cellular processes,such as signal transduction,ROS detoxification,energy pathway,protein synthesis and protein folding. While most of these salt-responsive putative phosphoproteins are known salt-stress-related proteins,some of them have not been previously reported. Our results provide not only new insights into salt stress responses in Thellungiella but also a good foundation for further investi-gation of these high salinity-regulated phosphoproteins.

关键词：蛋白质磷酸化盐芥盐生植物应力改建细胞信号转导蛋白质折叠蛋白质合成

Determination of Hydrocarbon Charging History by Diagenetic Sequence and Fluid Inclusions:A Case Study of the Kongquehe Area in the Tarim Basin

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Acta Geologica Sinica(English Edition) 2015年第3期89卷 876-886页

作者： WANG Bin FENG Yong ZHAO Yongqiang ZHOU Yushuang LUO Yu zhang genfa Wuxi Research Institute of Petroleum Geology SINOPEC School of Geosciences Yangtze University Research Institute of Exploration and Development Northwest Oilfield Branch CompanySINOPEC

Petrographic analysis of hydrocarbon inclusions in reservoirs is the basis and prerequisite for study of hydrocarbon charge history using fluid inclusion *** from Silurian reservoirs in the Kongquehe area were studied with microscopy,cathode luminescence and scanning electron microscopy,and the paragenetic sequence of diagenetic events was *** and oil inclusions were found in four different occurrences,i.e.,① in healed cracks in detrital quartz grains,② in quartz overgrowths that were formed relatively early in diagenesis,③ in healed cracks crosscutting quartz overgrowths and detrital quartz,and ④ in paragenetically late calcite *** bitumens were found in intergranular pores and in late fractures,whereas gas inclusions occur in healed cracks crosscutting quartz overgrowths and detrital *** homogenization temperatures of aqueous（Th_（aq）） and oil incluisons（Th_0） within individual fluid inclusion assemblages are very consistent,suggesting that the microthermometric data are *** Th_（aq） values are generally larger than Th_0,indicating the oil charging events took place at significant *** results suggest that there were at least two episodes of hydrocarbon charging in the Kongquehe area：the early hydrocarbon charging occurred in late Caledonian,dominated by oil,and the late hydrocarbon charging occurred in the Yanshan-Himalayan,first by oil and then *** addition,two episodes of hydrocarbon reservoir adjustment and destruction occurred in the Hercynian and Himalayan,respectively,forming solid bitumen.

关键词： Fluid inclusion diagenetic sequence hydrocarbon charge history Kongquehe area TarimBasin

Proteomic analysis of long-term salinity stress-responsive proteins in Thellungiella halophila leaves

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Chinese Science Bulletin 2008年第22期53卷 3530-3537页

作者： GAO Fei ZHOU YiJun HUANG LingYun HE DaChengt zhang genfa Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education College of Life Science Beijing Normal University Beijing 100875 China

Salinity is one of the most severe environmental factors that may impair crop productivity. A proteomic study based on two-dimensional gel electrophoresis is performed in order to analyze the long-term salinity stress response of Thellungiella halophila, an Arabidopsis-related halophyte. Four-week-old seedlings are exposed to long-term salinity treatment. The total crude proteins are extracted from leaf blades, separated by 2-DE, stained with Coomassie Brilliant Blue, and differentially displayed spots are identified by MALDI-TOF MS or QTOF MS/MS. Among 900 protein spots reproducibly detected on each gel, 30 spots exhibit significant change and some of them are identified. The identified proteins include not only some previously characterized stress-responsive proteins such as TIR-NBS-LRR class disease resistance protein, ferritin-1, and pathogenesis-related protein 5, but also some proteins related to energy pathway, metabolism, RNA processing and protein degradation, as well as proteins with unknown functions. The possible functions of these proteins in salinity tolerance of T. halophila are discussed and it is suggested that the long-term salinity tolerance of T. halophila is achieved, at least partly, by enhancing defense system, adjusting energy and metabolic pathway and maintaining RNA structure.

关键词：盐化作用蛋白质质谱学细胞学

RLINl,encoding a putative coproporphyrinogen Ⅲ oxidase,is involved in lesion initiation in rice

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Journal of Genetics and Genomics 2011年第1期38卷 29-37页

作者： Changhui Sun Linchuan Liu Jiuyou Tang Aihong Lin Fantao zhang Jun Fang genfa zhang Chengcai Chu Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education College of Life Science Beijing Normal University Beijing 100875 China The State Key Laboratory of Plant Genomics and National Plant Gene Research Center (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Datun Road Chaoyang District Beijing 100101 China Graduate School of the Chinese Academy of Sciences Yuquan Road Beijing 100039 China Rice Research Institute Sichuan Agricultural University Chengdu 611130 China

Lesion mimic is necrotic lesions on plant leaf or stem in the absence of pathogenic infection, and its exact biological mechanism is varied. By a large-scale screening of our T-DNA mutant population, we identified a mutant rice lesion initiation 1 （rlin1）, which was controlled by a single nuclear recessive gene. Map-based cloning revealed that RLIN1 encoded a putative coproporphyrinogen Ⅲ oxidase in tetrapyrrole biosynthesis pathway. Sequencing results showed that a G to T substitution occurred in the second exon of RLIN1 and led to a missense mutation from Asp to Tyr. Ectopic expression of RLIN1 could rescue rlin1 lesion mimic phenotype. Histochemical analysis demonstrated that lesion formation in rlin1 was light-dependent accompanied by reactive oxygen species accumulated. These results suggest that tetrapyrrole participates in lesion formation in rice.

关键词： Rice Map-based cloning Lesion mimic Coproporphyrinogen Ⅲ oxidase Tetrapyrrole

Biochemistry and Genetic Analysis of Bioeffects of Low Energy N+Implantation and Y-Radiation on Arabidopsis thaliana

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Frontiers in Biology 2006年第1期1卷 41-45页

作者： Shi Xiaoming Li Ke Nie Yanli zhang genfa College of Life Sciences Beijing Normal UniversityBeijing 100875China

We analyzed the activities of specific enzymes,esterase and peroxidase,and the electrophoresis profiling of isozymes of both the second-generation(M1)and the thirdgeneration(M2)individuals of Arabidopsis thaliana after the parental generation was treated with low-energy N+implantation andγ-*** indicated that the mutagenic effects of both treatments were *** obvious difference between N+implantation andγ-radial on the relationship of doses and effects was also *** the correlation between effects and doses,the former presented a particular saddle-like pattern,while the latter showed a pattern of *** amplification of polymorphic DNA analysis showed that the variant DNA bands were consistent in M1 and M2 generations treated with bothγ-radiation and ion ***,the ratio of individual plant variation between the two treatments was remarkably different.

关键词： ion implantation γ-ray isozyme Arabidopsis thaliana RAPD

DETERMINATION FREE PROLINE CONTENT OF JOJOBA LEAVES IN DROUGHT TREATMENTS

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DETERMINATION FREE PROLINE CONTENT OF JOJOBA LEAVES IN DROUG...

生物学前沿与进展开放课程建设的价值意蕴与实践探索

全国作物生物技术与诱变技术学术研讨会

作者： GENG Hongwei, zhang genfa(Life Science College, Beijing Normal University, 100875)

＜正＞ The relationship between the proline content and the drought resistance ofjojoba were investigated in this paper. The results indicated that the proline content in leaf can reflect the extent of the water loss in jojoba leaf, so that it is an easy way to determine the drought tolerance in jojoba. Jojoba mature leaves with similar figure were taken offas material in the experiment. Mature leaves refers to the second or third pair of leaves from the top of middle strong branches, their color was lighter than most of those leaves located in the middle and the bottom of a branch, but darker than the top or the second pair young leaves. All the leaves were 6-7cm long and 2.5-3.5cm wide with smooth surface.

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生物学杂志 2022年第2期39卷 122-124页

作者：邴杰张根发北京师范大学生命科学学院北京100875

信息技术的发展为高校研究生开放课程的建设提供了契机,生物学前沿与进展是针对生物学研究生开设的一门重要的专业基础课程,借助“互联网+”技术,将高校生物学前沿与进展研究生课程进行线上线下融合建设具有重要的价值意蕴,主要体现在:... 详细信息

信息技术的发展为高校研究生开放课程的建设提供了契机,生物学前沿与进展是针对生物学研究生开设的一门重要的专业基础课程,借助“互联网+”技术,将高校生物学前沿与进展研究生课程进行线上线下融合建设具有重要的价值意蕴,主要体现在:为研究生的自主学习提供重要契机,为创新型人才的培养提供全新的教学模式,为研究型人才科学思维的发展提供重要平台。基于前期建设经验,课程建设需要根据人才培养目标制定切实可行的实施计划,通过挖掘专业亮点,赋予线上线下相融合的开放课程创意和特色;围绕课程核心内容,确定线上线下教学活动。

关键词：生物学前沿与进展研究生课程线上线下课程建设

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植物转座元件及其表观遗传调控

北京师范大学学报（自然科学版） 2015年第1期51卷 54-59页

作者：于海婵张桂芳张根发北京师范大学生命科学学院北京100875 中国科学院生物物理研究所北京100101 北京师范大学学报(自然科学版)编辑部北京100875

植物的转座元件对于植物基因组的组成、进化和基因表达都具有重要影响.植物绝大部分的转座元件都处于沉默状态.植物机体通过一套识别转座元件并进行表观遗传沉默的有效机制,可逆地调控着转座元件的激活与沉默.本文综述了转座元件沉默和... 详细信息

植物的转座元件对于植物基因组的组成、进化和基因表达都具有重要影响.植物绝大部分的转座元件都处于沉默状态.植物机体通过一套识别转座元件并进行表观遗传沉默的有效机制,可逆地调控着转座元件的激活与沉默.本文综述了转座元件沉默和激活的表观遗传机制.

关键词：转座元件转座子表观遗传表观遗传机制

植物组蛋白赖氨酸化修饰参与基因表达调控的机理

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遗传 2014年第3期36卷 208-219页

作者：施子晗李泽琴张根发北京师范大学生命科学学院北京100875

组蛋白共价修饰作为表观遗传修饰的重要部分,主要包括乙酰化和甲酰化、甲基化、磷酸化、泛素化和SUMO化等,它们形成一个复杂的网络共同调控基因的表达,其中组蛋白甲基化修饰成为研究的热点,甲基化主要发生在赖氨酸残基上。近年来,随着... 详细信息

组蛋白共价修饰作为表观遗传修饰的重要部分,主要包括乙酰化和甲酰化、甲基化、磷酸化、泛素化和SUMO化等,它们形成一个复杂的网络共同调控基因的表达,其中组蛋白甲基化修饰成为研究的热点,甲基化主要发生在赖氨酸残基上。近年来,随着有关植物组蛋白赖氨酸甲基化修饰研究的不断深入,发现其通过改变自身赖氨酸残基的甲基化状态和甲基化程度,形成转录激活或者转录抑制标记,调控基因的表达,在植物开花和逆境胁迫的响应过程中起着至关重要的作用。H3组蛋白的赖氨酸甲基化修饰能够调控FLC基因和有关抗性基因的表达,具体表现为:H3K4的三甲基化促进FLC的表达,H3K27的三甲基化则抑制FLC的表达;H3K4me3作为转录激活标记,可激活PtdIns5P基因的表达,启动响应干旱的脂质合成信号通路,响应干旱胁迫;相反,H3K27me3作为一种转录抑制标记,低水平的H3K27me3诱导COR15A和ATGOLS3基因表达,它们分别编码叶绿体低温保护蛋白Cor15am和肌醇半乳糖合成酶GOLS,以抵抗寒冷胁迫。文章主要综述了植物组蛋白赖氨酸甲基化修饰参与DNA甲基化、开花过程以及应答逆境胁迫的分子机制。

关键词：植物组蛋白修饰赖氨酸甲基化基因表达调控