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Measures of bioavailable serum sex hormone levels in aging Chinese by protein chip

Science china(Life Sciences) 2006年第3期49卷 286-292页

作者： ZHOU Yong, CHANG Shuying, MENG Xiaoluo, YU Huafeng, WANG Luning, HE Jinggui, ZHANG Baohe, ZHANG Juntian, GENG Meiyu & DU Guanhua Institute of Marine Drugs and Food, Ocean University of china, Qingdao 266003, china Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, beijing 100050, china general hospital of air force, pla, beijing 100036, china 304 hospital of pla, beijing 100037, china beijing Tongren hospital, beijing 100730, china general hospital of pla, beijing 100853, china Institute of Marine Drugs Food Ocean University of China Qingdao 266003 China Institute of Materia Medica Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100050 China general hospital of air force PLA Beijing 100036 China 304 hospital of pla Beijing 100037 China beijing Tongren hospital Beijing 100730 China general hospital of pla Beijing 100853 China

The purpose of this study was to develop a protein chip technique based on receptor binding assays to measure bioavailable serum sex hormone levels (BSSHL). 224 aging healthy Chinese were investigated to get the referenced values of BSSHL for the first time. In the assays recombined sex hormone receptor proteins were jointed to polysaccharide coated slides to make protein chip,; the dose-dependence curves of sex hormone on chip were prepared. The data showed that this method had good precision (CV>16%); accuracy (Bias>10%),; the sensitivity could reach 1 pmol/L. From the results, BSSHL of men; women declined with aging, but no significant differences were observed. The BSSHL of aging men were higher than those of women. The bioavailable serum;rogen level of men was 52–112 pmol/L, women’s was 3–70 pmol/L; the whole group was 41.9–81.4 pmol/L. The bioavailable serum estrogen level of men was 0.8–3.0 pmol/L, women’s was 1.2–2.5 pmol/L; the whole group was 0.6–2.64 pmol/L. Based on the assays, BSSHL measurement by protein chip can meet the needs of epidemiological studies in terms of speed, accuracy; sample volume required,; was helpful in quantitative assessment of aging people’s health.

关键词： receptor protein chip estrogen - rogen aging

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糖尿病性牙周炎与氧化应激的研究进展

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牙体牙髓牙周病学杂志 2012年第8期22卷 484-488页

作者：韩春柯杰赵桂芝解放军空军总医院口腔科北京100036

糖尿病性牙周炎是糖尿病的并发症之一,由于糖尿病的存在,使得牙周炎病人牙周组织的破坏程度和病程进展速度都显著增高。近年来,氧化应激在糖尿病并发症中的作用机制日益得到重视,本文拟通过文献回顾对目前氧化应激在糖尿病性牙周炎中的... 详细信息

糖尿病性牙周炎是糖尿病的并发症之一,由于糖尿病的存在,使得牙周炎病人牙周组织的破坏程度和病程进展速度都显著增高。近年来,氧化应激在糖尿病并发症中的作用机制日益得到重视,本文拟通过文献回顾对目前氧化应激在糖尿病性牙周炎中的的研究进展作一综述。

关键词：糖尿病性牙周炎氧化应激血糖

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New development of biliary surgery in china

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World Journal of Gastroenterology 2000年第2期6卷 187-192页

作者： Zhi Qiang Huang The Research Institute of general Surgery,The general hospital of pla,beijing 100853,china 解放军总医院北京

CHARACTERISTICS OF BILIARY CALCULOUSDISEASES IN china:THE CHANGING SCOPEDiseases of the biliary tract in china is complicatedwith the prevalence of primary infection of the bileduct *** the middle of the 20th century,biliary infection,biliary parasitic infestation,andbiliary stones made up the three chief componentsof biliary diseases in *** to the

关键词： biliary surgery cholelithiasis intrahepatic stones carcinoma gallbladder carcinoma bile duct

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Silencing phospholipid scramblase 1 expression by RNA interference in colorectal cancer and metastatic liver cancer

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Hepatobiliary & Pancreatic Diseases International 2012年第4期11卷 393-400页

作者： Wei Cui,Shi-Yong Li,Jun-Feng Du,Zi-Man Zhu and Ping An Department of general Surgery,general Surgery Center of the pla,general hospital of beijing Military Command,beijing 100700,china Department of Hepatobiliary Surgery,First Affiliated hospital,general hospital of the pla,beijing 100037,china [a] Department of general Surgery General Surgery Center of the PLA General Hospital of Beijing Military Command Beijing 100700 China [b] Department of Hepatobiliary Surgery First Affiliated Hospital General Hospital of the PLA Beijing 100037 China

BACKGROUND:Phospholipid scramblase 1(PLSCR1) not only participates in the transbilayer movement of phospholipids,but also plays a role in the pathogenesis and progression of *** present study aimed to evaluate the effect of silencing PLSCR1 expression by RNA interference in colorectal cancer(CRC) and metastatic liver ***:The expression of PLSCR1 in CRC and metastatic liver cancer samples was assessed by *** cultured cells with the highest expression were selected for subsequent *** designed three siRNA oligonucleotide segments targeted at *** transfection was *** biological behavior of the cells in proliferation,adhesion,migration and invasion was ***:PLSCR1 protein expression increased significantly in the majority of CRC and metastatic liver cancer samples compared with normal *** cells had the highest expression of *** siRNA-390 oligonucleotide segment had the best silencing *** transfection,Lovo cell proliferation was significantly inhibited compared with the controls in the MTT *** and fibronectin adhesion assays showed Lovo cell adhesion was also significantly *** the migration assay,the number of migrating cells in the PLSCR1 siRNA-390 group was 50±12,significantly lower than the number in the siRNA-N group(115±28) and in the control group(118±31).In an invasion test,the number of invading cells in the PLSCR1 siRNA-390 group was 60±18,significantly lower than that in the siRNA-N group(97±26) and the control group(103±24).CONCLUSIONS:PLSCR1 is overexpressed in CRC and metastatic liver *** of PLSCR1 by siRNA inhibits the proliferation,adhesion,migration and invasion of Lovo cells,which suggests that PLSCR1 contributes to the tumorigenesis and tumor progression of ***1 may be a potential gene therapy target for CRC and associated metastatic liver cancer.

关键词： phospholipid scramblase 1 colorectal cancer metastatic liver cancer RNA interference

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Experi mental Study on Preparation of Decellularized Artery Vascular Graftand Transplantation of Carotid Artery Allografts

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生物医学工程学杂志 2005年第S1期22卷 63-64页

作者： Heng-Hua FAN~(1Δ) Bo-Xun ZHANG~2 Xiang-Dang LIANG~2 Ai-Y WANG~2 Di WU~1 Ji WU~11(Orthopedic Department of air force general hospital, beijing 100036,china.)2(Orthopedic Department of pla general hospital, beijing 100853,china)

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The ratio of MMP-2 to TIMP-2 in hilar cholangiocarcinoma:a semi-quantitative study

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Hepatobiliary & Pancreatic Diseases International 2004年第4期3卷 599-602页

作者： Mei Xiao, Nin-Xing Zhou,Zhi-Qiang Huang,Ya-Li Lu, Ling-Hong Chen, Dian-Jun Wang and Wei-Lin Chang beijing, china Department of Hepatobiliary Surgery, general air- force of hospital, beijing 100036, china and general hospital of People’ s Liberation Army, beijing 100853 , china Department of Hepatobiliary Surgery of general airforce of hospital Beijing 100036 China. fengweiairforce@***

BACKGROUND: Hilar cholangiocarcinoma is associated with low resectability and poor survival. The aim of this study was to evaluate the roles of matrix metalloproteinases- 2 (MMP-2 ) and its tissue inhibitor of metalloproteinase-2 (TIMP-2) in tumor invasion or as a prognostic factor in patients with human hilar cholangiocarcinoma. METHODS: The expressions of MMP-2 and TIMP-2 were investigated in patients. Paraffinized tissue sections ob- tained from 50 patients with human hilar cholangiocarcino- ma were analysed. The expressions of MMP-2 and TIMP-2 were examined immunohistochemically. Image analysis with image-pro plus analysis software was used to semi- quantitatively determine the ratio of MMP-2 to TIMP-2. RESULTS: The expression levels of MMP-2 and TIMP-2 were strongly associated with tumor hepatic invasion in pa- tients with hilar cholangiocarcinoma. Significant diffe- rences in the ratio of MMP-2 to TIMP-2 between some pathologic factors were observed in patients with hilar cholangiocarcinoma. CONCLUSIONS: MMP-2 plays an essential role in tumor invasion and metastasis,while TIMP-2 is shown to strongly inhibit cancer invasion and metastasis. The ratio of MMP-2 to TIMP-2 may be a prognostic indicator for patients with hilar cholangiocarcinoma.

关键词： hilar cholangiocarcinoma matrix metalloproteinase-2 tissue inhibitor of metalloproteinase-2 immunohistochemical staining tumor invasion prognostic factor

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Human hepatopoietin augmenter of liver regeneration──A hepatotrophic factor for liver regeneration, and its potential antihepatitis effect in vivo

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Chinese Science Bulletin 1998年第12期43卷 1026-1031页

作者： YANG Xiaoming 1, WANG Aimin 2, ZHOU Ping 3, XIE Ling 1, WANG Qingming 1, WU Zuze 1 and HE Fuchu 1 1. Institute of Radiation Medicine, beijing 100850, china 2. No. 252 hospital of pla, Baoding 071000, china 3. general hospital of the air Institute of Radiation Medicine Beijing China No. 252 hospital of pla Baoding China general hospital of the air force of pla Beijing China

The effect of human hapatopoietin i.e. augmenter of liver regeneration (hALR) was determined on hepatocyte DNA synthesis, and on CCl 4-induced hepatitis in animal in vitro and in vivo. It is found that ALR could directly stimulate DNA synthesis of hepatocytes in primary culture in a dose-dependent manner. In 30% hepatectomied rats, significant DNA synthesis occurred in control rats ; even so, exogenous hALR gene encoding protein increased DNA synthesis of regeneration liver by 2.3 fold compared with control rats. A lower dose of CCl 4 was administrated in rats and the effect of ALR on DNA synthesis of regenerating liver 48 h after CCl 4 administration was analyzed. Few Budr-labeled hepatocytes were visible in control rats. However, exogenous hALR markedly increased the number of labeled cells in a dose-dependent manner. Statistical analysis showed that 10 or 40 μg·kg -1 ALR protein stimulated DNA synthesis by 1.7 and 4.8 fold respectively. In addition to enhancing cell growth, adiministration of hALR achieved a significant improvement in reversing the lethality of rat hepatic failure when compared with that of control group; the elevation of cytosolic enzymes was dramatically suppressed by exogenous hALR in CCl 4-treated mice in vivo and in vitro; histologically, hepatocytes around the central vein were necrotic, and the degree of hepatocyte necrosis in control mice was more prominent than that in the mice given 40 μg·kg-1 hALR 48 h after CCl 4 administration. We also noted that hALR had a strong antihepatitis effect in vitro which was determined with primary cultured rat hepatocytes. These findings suggest that hALR protects the integrity of hepatocytes against severe hepatitis, and indicate that ALR may be an important regulator of liver regeneration and play a major role in liver injury repair. It proves ALR adminstration to be a useful treatment to accelerate liver regeneration and to prevent the onset of hepatitis or intrahepatic cholestasis induced by toxin.

关键词： liver regeneration hepatopoietin DNA synthesis hepatitis.

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Neural precursors derived from human embryonic stem cells

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Science china(Life Sciences) 2005年第3期48卷 295-299页

作者： Peng Hongmei 1,2 & Chen Gui’an 1 1. Department of Obstetrics and Gynecology, Peking University Third hospital, beijing 100083, china 2. Department of Obstetrics and Gynecology, general hospital of pla, beijing 100853, china 1. Department of Obstetrics and Gynecology Peking University Third Hospital 100083 Beijing China 2. Department of Obstetrics and Gynecology General Hospital of PLA 100853 Beijing China

Human embryonic stem (hES) cells provide a promising supply of specific cell types for transplantation therapy. We presented here the method to induce differentiation of purified neural precursors from hES cells. hES cells (Line PKU-1 and Line PKU-2) were cultured in sus- pension in bacteriological Petri dishes, which differentiated into cystic embryoid bodies (EBs). The EBs were then cultured in N2 medium containing bFGF in poly-L-lysine-coated tissue culture dishes for two weeks. The central, small cells with 2―3 short processes of the spreading out- growth were isolated mechanically and replated. The resulting neurospheres were cultured in suspension for 10 days, then dissociated into single cell suspension with a Pasteur pipette and plated. Cells grew vigorously in an attached way and were passed every 4―5 days. Almost all the cells were proved nestin positive by immunostaining. Following withdrawal of bFGF, they differentiated into neurons expressing β-tubulin isotypeIII, GABA, serotonin and synaptophysin. Through induction of PDGF-AA, they differentiated into astrocytes expressing GFAP and oli- godendrocytes expressing O4. The results showed that hES cells can differentiate into typical neural precursors expressing the specific marker nestin and capable of generating all three cell types of the central nervous system (CNS) in vitro.

关键词： human embryonic stem cell, differentiation, neural precursor.

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Detection of Early Noise-Induced Hearing Impairment in Pilots

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Journal of Otology 2008年第2期3卷 108-112页

作者： WANG En-tong, GONG Wei-xi, CHEN Wei, MA Xiao-li, XIA Hui, CHEN Jun Department of Otolaryngology-Head and Neck Surgery, Chinese pla air force general hospital, 30 Fucheng Road, beijing 100036, P.R.china Department of Otolaryngology–Head and Neck Surgery Chinese PLA Air Force General Hospital 30 Fucheng Road Beijing 100036 P.R. China

Objective To detect early signs of noise-induced hearing loss(NIHL) in military pilots without hearing complaints. Methods Pure tone audiometry and acoustic reflex thresholds were tested in 36 military pilots (72 ears) with noise exposure history but no complaints of hearing loss. Conventional test frequencies (0.25-8 kHz) and extended high frequencies (EHF, 10 and 12.5 kHz) were included in audiometry. White noise and pure tones at 0.5, 1, 2, and 4 kHz were used for acoustic reflex tests. Twenty normal hearing subjects(40 ears) with no exposure to occupational noise were used as controls. Results Pure tone thresholds at all conventional frequencies and at EHFs were elevated in the pilots, with the maximum shift at 4 kHz, compared with controls (p < 0.01). The pilots also showed elevated ART to white noise and decreased differentials between white noise and pure tone ARTs (p< 0.01). Conclusion Early signs of NIHL are present in some symptom-free military pilots. High frequency hearing threshold shift, elevated white noise ART and decreased differential between white noise and pure tone ARTs may be objective indicators of early NIHL.

关键词： Aviator Hearing loss High frequency audiometry Acoustic reflex threshold

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Infection associated with hydroxyapatite prosthesis and related factors

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Journal of Otology 2010年第1期5卷 24-29页

作者： LIU Yang SUN Jian-jun Center of Otolaryngology of pla, Navy general hospital, beijing 100037, china Center of Otolaryngology of pla Navy General Hospital Beijing 100037 China

Background The purpose of otomicrosurgery in modern times is to achieve complete removal of disease, which is the basis for hearing rehabilitation. Artificial ossicles are important for hearing rehabilitation. One of the materials is hydroxyapatite that acts as ossicular replacement prosthesis (HA prosthesis, PORP & TORP) and is commonly used as substitute bone in clinics. Although HA prostheses feature good biocompatibility, stability and certain resistance to infection, the structure of the material can be decomposed and absorbed. Based upon animal experiments and clinical application, this article explores the infectivity of HA prostheses and influencing factors. Materials and Methods HA prostheses were implanted in the bulla in guinea pigs. Infected HA prostheses were examined under a scanning electron microscope. Ninety patients implanted with HA prostheses were followed for more than 3 months. Five cases that had undergone revision operations were reported. Results Scanning electronic microscopy showed that the normal compact structures had disappeared and become loose in infected HA prostheses, with their micropores infiltrated with effusion, fibrous tissue and inflammatory cells. As a result, the normal ceramic structure was decomposed and the micropores structure disappeared. The infected mesotympanum in the reported cases also led to HA infection. The shape and structure of bone were destroyed, which was visible by visual examination and light microscopy. The process of cholesteatoma recurrence was always associated with infection, although the HA prosthesis was sometimes spared. Conclusion While HA prosthesis is anti-infective to some extent, long-term infection and cholesteatoma recurrence can lead to destruction and decomposition of HA prostheses. Long-term infected middle ear cavity, dysfunctional Eustachian tube, residual and recurrent cholesteatoma and other factors can cause decomposition of HA prosthesis and result in hearing loss.

关键词： Hydroxyapatite prosthesis Infection Cholesteatoma.

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