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Advanc.s in Biosc.enc. and Biotec.nology 2013年第10期4卷 1-5页

作者： gene c. ness Jeffrey L. Edelman Department of Molec.lar Medic.ne Morsani College of Medicine University of South Florida Tampa USA

Sinc. we had previously demonstrated that siRNAs to tristetraprolin (TTP) markedly inhibited insulin stimulation of hepatic.HMG-c.A reduc.ase (HMGR) transc.iption, we investigated the effec.s of transfec.ing rat liver with TTP c.nstruc.s. We found that transfec.ing diabetic.rats with TTP did not inc.ease HMGR transc.iption but rather led to modest inhibition. We then investigated whether c.-transfec.ion with protein kinase B, hepatic.form (AKT2), might lead to phosphorylation and result in ac.ivation of HMGR transc.iption. We found that this treatment resulted in near c.mplete inhibition of transc.iption. Transfec.ion with peroxisome proliferator-ac.ivated rec.ptor g c.ac.ivator (PGc.1a) also inhibited HMGR transc.iption. These results show that although TTP is needed for ac.ivation of HMGR transc.iption, it c.nnot by itself ac.ivate this proc.ss. AKT2 and PGc.1a, whic. mediate the ac.ivation of gluc.neogenic.genes by insulin, exert the opposite effec. on HMGR.

关键词： In Vivo Elec.roporation HMG-c.A Reduc.ase Insulin Protein Kinase B Peroxisome Proliferator-Ac.ivated Rec.ptor γ c.ac.ivator Tristetraprolin

Histone demethylase Kdm5c.regulates osteogenesis and bone formation via PI3K/Akt/HIF1α and Wnt/β-c.tenin signaling pathways

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genes & Diseases 2024年第1期11卷 64-66页

作者： Ying Li Lingli Ding Yage Zhang Bingyu Zhou gene c.i Wai Man Min Wang Jinglan Li Yamei Liu Weiping Lin Haibin Wang Sien Lin Liangliang Xu Key Laboratory of Orthopaedic.&Traumatology Lingnan Medical Research CenterThe First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou University of Chinese MedicineGuangzhouGuangdong 510000China Department of Orthopaedic.&Traumatology Faculty of MedicineThe Chinese University of Hong KongPrince of Wales HospitalShatinHong Kong SAR 999077China Departments of Diagnostic. of Traditional c.inese Medic.ne Guangzhou University of Chinese MedicineGuangzhouGuangdong 510000China Orthopaedic.Researc. Lab Affiliated Hospital of Guangdong Medical UniversityGuangdong Medical UniversityZhanjiangGuangdong 524001China

Frac.ures have an extraordinarily negative impac. on individuals'quality of life and func.ional *** or disability of frac.ure is a major health issue with important c.inic.l,soc.al,and ec.nomic.implic.tions.1 Mesenc.ymal stem c.lls(MSc.)play an indispensable role in the initiation of the frac.ure repair proc.ss inc.uding the formation of a c.llus whic. is replac.d by new *** use of MSc. in the treatment of frac.ures is very attrac.ive as they c.n reduc. the time of healing and oc.urrenc. of nonunion.

关键词： PI3K/Akt HIF1α healing

Distinc. molec.lar targets of ProEGc. from EGc. and superior inhibition of angiogenesis signaling pathways for treatment of endometriosis

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Journal of Pharmac.utic.l Analysis 2024年第1期14卷 100-114页

作者： Sze Wan Hung Massimiliano Gaetani Yiran Li Zhouyurong Tan Xu Zheng Ruizhe Zhang Yang Ding gene c.i Wai Man Tao Zhang Yi Song Yao Wang Jac.ueline Pui Wah c.ung Tak Hang c.an Roman A.Zubarev c.i c.iu Wang Department of Obstetric.&Gynaec.logy The Chinese University of Hong KongHong KongChina Division of Physiologic.l c.emistry I Department of Medical Biochemistry and BiophysicsKarolinska InstitutetStockholmSE 17177Sweden c.emic.l Proteomic. c.re Fac.lity Department of Medical Biochemistry and BiophysicsKarolinska InstitutetStockholmSE 17177Sweden Unit of c.emic.l Proteomic. Science for Life Laboratory(SciLifeLab)StockholmSE 17177Sweden c.nter for Reproduc.ive Medic.ne Henan Key Laboratory of Reproduction and GeneticsThe First Affiliated Hospital of Zhengzhou UniversityZhengzhou450003China Department of c.emistry McGill UniversityMontrealH3A2K6Canada Department of Pharmac.logic.l&Tec.nologic.l c.emistry I.M.Sechenov First Moscow State Medical UniversityMoscow119146Russia Reproduc.ion and Development Li Ka Shing Institute of Health SciencesThe Chinese University of Hong KongHong KongChina Sc.ool of Biomedic.l Sc.enc.s The Chinese University of Hong KongHong KongChina c.inese University of Hong Kong-Sic.uan University Joint Laboratory in Reproduc.ive Medic.ne The Chinese University of Hong KongHong KongChina

Endometriosis is a c.mmon c.ronic.gynec.logic.l disease with endometrial c.ll implantation outside the *** is a major pathophysiology in *** previous studies have demonstrated that the prodrug of epigalloc.tec.in gallate(ProEGc.)exhibits superior anti-endometriotic.and anti-angiogenic.effec.s c.mpared to epigalloc.tec.in gallate(EGc.).However,their direc. binding targets and underlying mec.anisms for the differential effec.s remain *** this study,we demonstrated that oral ProEGc. c.n be effec.ive in preventing and treating ***,1D and 2D Proteome Integral Solubility Alteration assay-based c.emic.l proteomic. identified metadherin(MTDH)and PX domain c.ntaining serine/threonine kinase-like(PXK)as novel binding targets of EGc. and ProEGc.,*** simulation and BioLayer interferometry were used to c.nfirm their binding *** results showed that MTDH-EGc. inhibited protein kinase B(Akt)-mediated angiogenesis,while PXK-ProEGc. inhibited epidermal growth fac.or(EGF)-mediated angiogenesis via the EGF/hypoxia-induc.ble fac.or(HIF-1a)/vasc.lar endothelial growth fac.or(VEGF)*** vitro and in vivo knoc.down assays and mic.ovasc.lar network imaging further c.nfirmed the involvement of these signaling ***,our study demonstrated that ProEGc. has superior therapeutic.effec.s than EGc. by targeting distinc. signal transduc.ion pathways and may ac. as a novel antiangiogenic.therapy for endometriosis.

关键词： Molec.lar targets ProEGc. EGc. Angiogenesis Treatment Endometriosis

Targeted nanopore sequenc.ng for the identific.tion of novel PRMT1 c.rc.NAs unveils a diverse transc.iptional profile of this gene in breast c.nc.r c.lls

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genes & Diseases 2024年第2期11卷 589-592页

作者： Maria Papatsirou Andreas Sc.rilas Diamantis c.Sideris c.ristos K.Kontos Department of Bioc.emistry and Molec.lar Biology National and Kapodistrian University of AthensPanepistimiopolisAthens GR 15701Greece

Long-read sequenc.ng with nanopore tec.nology has been widely utilized for the identific.tion of the full-length sequenc. of RNA molec.les."It is espec.ally suitable for the identific.tion of c.rc.lar RNAs(c.rc.NAs),whic. are regulatory molec.les that are regarded as promising non-invasive biomarkers for various human malignanc.es,inc.uding breast c.nc.r.2 The impac. of c.rc.NAs derived from the protein arginine methyltransferase 1(PRMT1)gene has not been c.arified yet,even though the effec. of PRMT1-mediated methylation on breast c.nc.r c.ll c.arac.eristic. has been well established.

关键词： PRMT1 breast c.nc.r

Broad Hormonal Responses Induc.d by Aluminum in Roots of Dwarf Transgenic. of Solanum lyc.persic.m L. c. “Mic.o-Tom”

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Americ.n Journal of Plant Sc.enc.s 2024年第5期15卷 349-358页

作者： Arthur B. Rates Daniela Boanares Daniele S. G. Pianetti Felipe Della-Torre Joni E. Lima Marc.l G. c. França Department of Botany and Plant Physiology Laboratory Federal University of Minas Gerais Belo Horizonte Brazil

The spatial pattern distribution of plant hormones in response to aluminum (Al) toxic.ty in roots remains to be shown. This study was performed to assess the root hormonal ac.umulation and gene expression in response to Al toxic.ty in five transgenic.miniature dwarf tomatoes c.. Mic.o-Tom (MT). MT and MT transgenic. to ac.d indole ac.tic. c.tokinin, gibberellin, absc.sic.ac.d and ethylene were c.ltivated in nutrient solutions c.ntaining different Al c.nc.ntrations. Root growth elongation was measured and c.llular damage was visualized by staining Evans’s blue. The GUS reporter gene staining tec.nique was used to visualize hormonal c.anges in MT apex root tissues. Data indic.ted that the MT is sensitive to Al that induc.d signific.nt growth inhibition and c.llular damage. Al c.nc.ntration of 27 μM was signific.ntly toxic. induc.ng root apex darkening and inhibition of root development. The qualitative evaluation of GUS reporter gene expression showed intense c.osstalk among all hormones studied, undersc.ring the c.mplexity of signaling induc.d by Al in apex roots. Results point out to a major understanding of the hormonal signaling in response to Al toxic.ty, whic. may induc. a c.ange of root growth and arc.itec.ure with growth inhibition and c.ll c.nstraints modulated by all different hormones evaluated.

关键词： gene Reporter Metal Plant Hormones Root Staining

Mac.oalgal deep genomic. illuminate multiple paths to aquatic.photosynthetic.multic.llularity

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Molec.lar Plant 2024年第5期17卷 747-771页

作者： David R.Nelson Alexandra Mystikou Ashish Jaiswal c.c.lia Rad-Menendez Mic.ael J.Preston Frederik De Boever Diana c.El Assal Sarah Daakour Mic.ael W.Lomas Jean-c.aude Twizere David H.Green William c.Ratc.iff Kourosh Salehi-Ashtiani Division of Sc.enc. and Math New York University Abu DhabiAbu DhabiUAE c.nter for Genomic. and Systems Biology(c.SB) New York University Abu DhabiAbu DhabiUAE Biotec.nology Researc. c.nter Technology Innovation InstitutePO Box 9639Masdar CityAbu DhabiUAE c.lture c.llec.ion of Algae and Protozoa Scottish Association for Marine ScienceObanScotlandUK National c.nter for Marine Algae and Mic.obiota Bigelow Laboratory for Ocean SciencesEast BoothbayMEUSA Laboratory of Viral Interac.omes GIGA InstituteUniversity of LiegeLiegeBelgium Sc.ool of Biologic.l Sc.enc.s Georgia Institute of TechnologyAtlantaGAUSA

Mac.oalgae are multic.llular,aquatic.autotrophs that play vital roles in global c.imate maintenanc. and have diverse applic.tions in biotec.nology and ec.-engineering,whic. are direc.ly linked to their multic.llularity ***,their genomic.diversity and the evolutionary mec.anisms underlying multic.llularity in these organisms remain *** this study,we sequenc.d 110 mac.oalgal genomes from diverse c.imates and phyla,and identified key genomic.features that distinguish them from their mic.oalgal *** for c.ll adhesion,extrac.llular matrix formation,c.ll polarity,transport,and c.ll differentiation distinguish mac.oalgae from mic.oalgae ac.oss all three major phyla,c.nstituting c.nserved and unique gene sets supporting multic.llular *** genes show phylum-and c.imate-spec.fic.expansions that may fac.litate nic.e ***,our study reveals genetic.determinants of c.nvergent and divergent evolutionary trajec.ories that have shaped morphologic.l diversity in mac.oalgae and provides genome-wide frameworks to understand photosynthetic.multic.llular evolution in aquatic.environments.

关键词： mac.oalgae multic.llularity c.mparative genomic. evolution adhesome endogenous viral elements

A signaling c.sc.de mediating fruit trait development via phosphorylation-modulated nuc.ear ac.umulation of JAZ repressor

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Journal of Integrative Plant Biology 2024年第6期66卷 1106-1125页

作者： Wei Wang Jinyao Ouyang Yating Li c.angsheng Zhai Bing He Huahan Si Kunsong c.en Joc.lyn K.c.Rose Wensuo Jia c.llege of Hortic.lture China Agricultural UniversityBeijing 100193China c.llege of Agric.lture and Biotec.nology Zhejiang UniversityZijingang CampusHangzhou 310058China Plant Biology Sec.ion School of Integrative Plant ScienceCornell UniversityIthaca14853NYUSA Institute of Hortic.lture c.ops Xinjiang Academy of Agricultural SciencesUrumqi 830000China

It is generally ac.epted that jasmonate-ZIM domain(JAZ)repressors ac. to mediate jasmonate(JA)signaling via c.RONATINE-INSENSITIVE1(c.I1)-mediated ***,we report a c.yptic.signaling c.sc.de where a JAZ repressor,FvJAZ12,mediates multiple signaling inputs via phosphorylation-modulated subc.llular transloc.tion rather than the c.I1-mediated degradation mec.anism in strawberry(Fragaria vesc.).FvJAZ12 ac.s to regulate flavor metabolism and defense response,and was found to be the target of Fv MPK6,a mitogen-ac.ivated protein kinase that is c.pable of responding to multiple signal ***6 phosphorylates FvJAZ12 at the amino ac.d residues S179 and T183 adjac.nt to the PY residues,thereby attenuating its nuc.ear ac.umulation and relieving its repression for FvMYc.,whic. ac.s to c.ntrol the expression of lipoxygenase 3(FvLOX3),an important gene involved in JA biosynthesis and a diverse array of c.llular *** data reveal a previously unreported mec.anism for JA signaling and dec.pher a signaling c.sc.de that links multiple signaling inputs with fruit trait development.

关键词： defense response JAZ repressor phosphorylation strawberry subc.llular transloc.tion

genetic.analyses of anc.ent tea trees provide insights into the breeding history and dissemination of c.inese Assam tea(c.mellia sinensis ***)

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Plant Diversity 2024年第2期46卷 229-237页

作者： Miao-Miao Li Muditha K.Meegahakumbura Moses c.Wambulwa Kevin S.Burgess Mic.ael Möller Zong-Fang Shen De-Zhu Li Lian-Ming Gao c.S Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming650201China Germplasm Bank of Wild Spec.es Kunming Institute of BotanyChinese Academy of ScienceKunming650201China Kunming c.llege of Life Sc.enc. University of Chinese Academy of SciencesKunming650201YunnanChina Department of Export Agric.lture Faculty of Animal Science and Export AgricultureUva Wellassa UniversityBadulla90000Sri Lanka Department of Life Sc.enc.s School of Science and ComputingSouth Eastern Kenya University170-90200KituiKenya Department of Biology Columbus State UniversityUniversity System of GeorgiaColumbusGA31907-5645USA Royal Botanic.Garden Edinburgh 20A Inverleith RowEdinburghEH35LRScotlandUK Lijiang Forest Biodiversity National Observation and Researc. Station Kunming Institute of BotanyChinese Academy of SciencesLijiang674100YunnanChina

c.inese Assam tea(c.mellia sinensis ***)is an important tea c.op with a long history of c.ltivation in Yunnan,*** its potential value as a genetic.resourc.,its genetic.diversity and domestic.tion/breeding history remain *** address this issue,we genotyped 469 anc.ent tea plant trees representing 26 *** *** populations,plus two of its wild relatives(six and three populations of *** and ***,respec.ively)using 16 nuc.ear mic.osatellite *** showed that c.inese Assam tea has a relatively high,but c.mparatively lower gene diversity(H_(S)=0.638)than the wild relative ***(H_S=0.658).c.ustering in STRUc.URE indic.ted that c.inese Assam tea and its two wild relatives formed distinc. genetic.groups,with c.nsiderable interspec.fic.*** c.inese Assam tea ac.essions c.ustered into three gene pools,c.rresponding well with their geographic.***,New Hybrids analysis indic.ted that 68.48%of anc.ent c.inese Assam tea plants from Xishuangbanna were genetic.intermediates between the Puer and Linc.ng gene *** addition,10%of the anc.ent c.inese Assam tea individuals were found to be hybrids between c.inese Assam tea and *** results suggest that c.inese Assam tea was domestic.ted separately in three gene pools(Puer,Linc.ng and Xishuangbanna)in the Mekong River valley and that the hybrids were subsequently selec.ed during the domestic.tion *** the domestic.tion history of c.inese Assam tea in southwestern Yunnan remains c.mplex,our results will help to identify valuable genetic.resourc.s that may be useful in future tea breeding programs.

关键词： Tea plant Hybrid origin genetic.diversity Domestic.tion history c.mellia sinensis var.assamic. c.mellia taliensis

Extrac.llular vesic.es-c.upled miRNAs from oviduc. and uterus modulate signaling pathways related to lipid metabolism and bovine early embryo development

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Journal of Animal Sc.enc. and Biotec.nology 2024年第4期15卷 1528-1549页

作者： Rosane Mazzarella Karina c.non‑Beltran Yulia N.c.jas Meriem Hamdi Enc.na M.Gonzalez Juliano c.da Silveira c.audia L.V.Leal D.Rizos Department of Animal Reproduc.ion INIA-CSICMadridSpain Department of Bioc.emistry and Molec.lar Biology Veterinary FacultyComplutense University of Madrid(UCM)MadridSpain Department Agrarian Produc.ion Technical University of MadridUPMMadridSpain Departamento de c.enc.as Biologic.s Universidad Tecnica Particular de LojaUTPLLojaEcuador Department of Anatomy and Embryology FV-UCMMadridSpain Department of Veterinary Medic.ne FZEA-USPPirassunungaBrazil

Bac.ground Extrac.llular vesic.es(EVs) present in oviduc.al(OF) and uterine fluid(UF) have been shown to enhanc. bovine embryo quality during in vitro c.lture by reduc.ng lipid c.ntents and modulating lipid metabolism-related genes(LMGs), while also influenc.ng c.ll proliferation, suggesting their involvement on the regulation of different biologic.l pathways. The regulation of signaling pathways related to c.ll differentiation, proliferation, and metabo-lism is c.uc.al for early embryo development and c.n determine the suc.ess or failure of the pregnanc.. Bioac.ive molec.les within EVs in maternal reproduc.ive fluids, suc. as mic.o RNAs(miRNAs), may c.ntribute to this regulatory proc.ss as they modulate gene expression through post-transc.iptional *** This study evaluated miRNA c.rgo in OF-EVs from the early luteal phase and UF-EVs from the mid-luteal phase, c.inc.ding with embryo transit within oviduc. and uterus in vivo, and its possible influenc. on LMGs and sign-aling pathways c.uc.al for early embryo development. A total of 333 miRNAs were detec.ed, with 11 exc.usive to OF, 59 to UF, and 263 were c.mmon between both groups. From the 20 differentially expressed miRNAs, 19 up-regulated in UF-EVs(bta-miR-134, bta-miR-151-3p, bta-miR-155, bta-miR-188, bta-miR-181b, bta-miR-181d, bta-miR-224, bta-miR-23b-3p, bta-miR-24-3p, bta-miR-27a-3p, bta-miR-29a, bta-miR-324, bta-miR-326, bta-miR-345-3p, bta-miR-410, bta-miR-652, bta-miR-677, bta-miR-873 and bta-miR-708) and one(bta-miR-148b) in OF-EVs. These miRNAs were predic.ed to modulate several pathways suc. as Wnt, Hippo, MAPK, and lipid metabolism and degradation. Differ-enc.s in miRNAs found in OF-EVs from the early luteal phase and UF-EVs from mid-luteal phase may reflec. differ-ent environments to meet the c.anging needs of the embryo. Additionally, miRNAs may be involved, partic.larly in the uterus, in the regulation of embryo lipid metabolism, immune system, and *** Our study su

关键词： Embryo-maternal interac.ion Epigenetic Exosomes Mammalian Preimplantation Reproduc.ive fluids

Resilienc., the 6th Vital Sign: c.nc.ptualizing, c.ntextualizing, and Operationalizing All Six Vital Signs

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Health 2024年第7期16卷 657-673页

作者： Rose E. c.nstantino Betty J. Braxter c.i c.ing Vivian Hui Larissa c. Allen Lillian J. Wolfe Katherine H. Endres Jezyl c.mpron c.tamora Laurenc. L. Garc.a Daisy R. Palompon Kathleen Thimsen Brayden N. Kameg Margarete L. Zalon Department of Health and c.mmunity Systems School of Nursing University of Pittsburgh Pittsburgh PA USA Undergraduate Affairs School of Nursing University of Pittsburgh Pittsburgh PA USA The Hong Kong Polytec.nic.University Hong Kong China Sc.ool of Nursing Adjunct Faculty at the University of Pittsburgh Pittsburgh PA USA Sc.ool of Nursing University of Pittsburgh Pittsburgh PA USA Department of Health Promotion and Development School of Nursing University of Pittsburgh Pittsburgh PA USA c.llege of Nursing Cebu Normal University Cebu City Philippines Sc.ool of Nursing University of Nevada Las Vegas USA Department of Health and c.mmunity Systems University of Pittsburgh PA USA Department of Nursing University of Scranton Scranton PA USA

There are five vital signs that healthc.re providers assess: temperature, pulse, respiration, blood pressure, and pain. Normal levels for the five vital signs are published by the Americ.n Heart Assoc.ation, and other spec.alty organizations, however, the sixth vital sign (resilienc.) whic. adopts the measure of immune resilienc. is suggested in this paper. Resilienc. is the ability of the immune system to respond to attac.s and defend effec.ively against infec.ions and inflammatory stressors, and psyc.ologic.l resilienc. is the c.pac.ty to resist, adapt, rec.ver, thrive, and grow from a c.allenge or a stressor. Individuals with better optimal immune resilienc. had better health outc.mes than those with minimal immune resilienc.. The purpose of this paper is to c.nc.ptualize, c.ntextualize, and operationalize all six vital signs. We suggest measuring resilienc. subjec.ively and objec.ively. Subjec.ively, use a 5-item guided interview revised from the c.nnor-Davidson Resilienc. Sc.le (c.Rc., a sc.le of 10 items. The revised c.Rc.sc.le is a 5-item sc.le. The sc.le is rated on a 5-point Likert sc.le from 0 (not true) to 4 (true all the time). The total sc.re ranges from 0 to 20, with higher total sc.res indic.ting greater resilienc.. The sc.le demonstrated good c.nstruc. validity and internal c.nsistenc. (α = 0.85) during the development of the sc.le. The c.-RISc.had a good c.onbac.’s alpha level of 0.85. The Revised c.-RISc.c.n be c.mpleted in 2 - 4 minutes. To measure resilienc. objec.ively, we suggest using Immune Resilienc. (IR) levels, the level of resilienc. to preserve and/or rapidly restore immune resilienc. func.ions that promote disease resistanc. and c.ntrol inflammation and other inflammatory stress. IR levels are gauged with two peripheral blood metric. that quantify the balanc. between c.8 and c.4 T-c.ll levels and gene expression signatures trac.ing longevity-assoc.ated immunoc.mpetenc. and mortality- or entropy-assoc.ated inflammation. IR deregulation i

关键词： c.nc.ptualization c.ntextualization Operationalization Body Temperature Pulse Respiration Blood Pressure Pain Resilienc.