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Microbial Fertilizer: A Sustainable Strategy for Medicinal Plants Production

Phyton-International Journal of Experimental Botany 2024年第6期93卷 1221-1236页

作者： Chuang Liu Jing Xie Hao Liu Can Zhong gen pan Shuihan Zhang Jian Jin Hunan Academy of Chinese Medicine Hunan University of Chinese MedicineChangsha410036China Institute of Chinese Medicine Resources Hunan Academy of Chinese MedicineChangsha410013China

Medicinal plants have aroused considerable interest as an alternative to chemical drugs due to the beneﬁcial effects of their active secondary metabolites.However,the extensive use of chemical fertilizers and pesticides in pursuit of yield has caused serious pollution to the environment,which is not conducive to sustainable devel-opment in theﬁeld of medicinal plants.Microbial fertilizers are a type of“green fertilizer”containing speciﬁc microorganisms that can improve the soil microbial structure,enhance plant resistance to biological and abiotic stresses,and increase the yield of medicinal plants.The root exudates of medicinal plants attract different micro-organisms to the rhizosphere,which then migrate further to the plant tissues.These microbes can increase the levels of soil nutrients,and improve the physical and chemical properties of soil through nitrogenﬁxation,and phosphorus and potassium solubilization.In addition,soil microbes can promote the synthesis of hormones that increase plant growth and the accumulation of active compounds,eventually improving the quality of med-icinal plants.In 2022,the total value of the global microbial fertilizer market was$4.6 billion and is estimated to reach$10.36 billion by 2030.In this review,we have summarized the types of microbial fertilizers,the coloniza-tion and migration of microorganisms to plant tissues,and the beneﬁcial effects of microbial fertilizers.In addi-tion,the prospects of developing microbial fertilizers and their application for medicinal plants have also been discussed.It aims to provide a reference for the rational application of microbial fertilizers in theﬁeld of med-icinal plants and the green and sustainable development of medicinal plant resources.

关键词： Microbial fertilizers market overview rhizosphere microorganisms endophytic bacteria beneﬁcial effects

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DDG1 and G Protein α Subunit RGA1 Interaction Regulates Plant Height and Senescence in Rice(Oryza sativa)

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Phyton-International Journal of Experimental Botany 2023年第7期92卷 2051-2064页

作者： Xi Liu Chuxuan Zhao Di Wang gen pan Xiaonan Ji Su Gao Tanxiao Du Yating Feng Wenjing Chen Jiangsu Key Laboratory of Eco-Agricultural Biotechnology around Hongze Lake Regional Cooperative Innovation Center for Modern Agriculture and Environmental ProtectionHuaiyin Normal UniversityHuai’an223300China School of Life Sciences Huaiyin Normal UniversityHuai’an223300China Crop Research and Development Center Huaiyin Institute of Agricultural Sciences of Xuhuai Region in JiangsuHuai’an223001China Institute of Bast Fiber Crops Chinese Academy of Agricultural SciencesChangsha410205China

Many studies have already shown that dwarfism and moderate delayed leaf senescence positively impact rice yield,but the underlying molecular mechanism of dwarfism and leaf senescence remains largely unknown.Here,using map-based cloning,we identified an allele of DEP2,DDG1,which controls plant height and leaf senescence in rice.The ddg1 mutant displayed dwarfism,short panicles,and delayed leaf senescence.Compared with the wild-type,ddg1 was insensitive to exogenous gibberellins(GA)and brassinolide(BR).DDG1 is expressed in various organs,especially in stems and panicles.Yeast two-hybrid assay,bimolecular fluorescent complementation and luciferase complementation image assay showed that DDG1 interacts with theα-subunit of the heterotrimeric G protein.Disruption of RGA1 resulted in dwarfism,short panicles,and darker-green leaves.Furthermore,we found that ddg1 and the RGA1 mutant was more sensitive to salt treatment,suggesting that DDG1 and RGA1 are involved in regulating salt stress response in rice.Our results show that DDG1/DEP2 regulates plant height and leaf senescence through interacting with RGA1.

关键词： Oryza sativa DDG1 plant height senescence RGA1

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数字经济赋能高质量充分就业:内在逻辑、作用机理与优化路径

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华北水利水电大学学报（社会科学版） 2024年第5期40卷 1-9页

作者：刘盘根高晋华北水利水电大学公共管理学院河南郑州450046 华北水利水电大学城乡融合发展研究中心河南郑州450046

实现高质量充分就业是党的二十大提出的重要目标。数字经济赋能高质量充分就业为增进民生福祉、提高人民生活品质、解决就业问题提供新动力。数字经济发展与实现高质量充分就业存在内在逻辑,二者在实现全体人民共同富裕方面具有目标一致... 详细信息

实现高质量充分就业是党的二十大提出的重要目标。数字经济赋能高质量充分就业为增进民生福祉、提高人民生活品质、解决就业问题提供新动力。数字经济发展与实现高质量充分就业存在内在逻辑,二者在实现全体人民共同富裕方面具有目标一致性,数字经济为实现高质量充分就业提供有力支撑,同时,高质量充分就业也是数字经济发展的重要驱动力。数字经济赋能高质量充分就业的作用机理可归纳为数字技术赋能、组织载体赋能、数字产业赋能、服务供给赋能四个维度。在新发展阶段,数字经济赋能高质量充分就业需要从以下几个方面协同推进:健全劳动法律法规;培育数字经济新业态;加强数字经济就业服务平台建设;构建就业评价指标体系。

关键词：数字经济数字赋能高质量充分就业

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Significant contribution of carbonyls to atmospheric oxidation capacity(AOC)during the winter haze pollution over North China Plain

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Journal of Environmental Sciences 2024年第5期139卷 377-388页

作者： Xue Yang gen Zhang Guang pan Guolan Fan Houyong Zhang Xuan Ge Mingyue Du School of Municipal and Environmental Engineering Shandong Jianzhu UniversityJi’nan 250101China State Key Laboratory of Severe Weather&Key Laboratory of Atmospheric Chemistry of China Meteorological Administration Chinese Academy of Meteorological SciencesBeijing 100081China Shandong Jinan Ecological Environment Monitoring Center Ji’nan 250101China

Atmospheric carbonyl compounds play significant roles in the cycling of radicals and have exhibited surprisingly high levels in winter that were well correlated to particulate matter,for which the reason have not been clearly elucidated.Here we measured carbonyl compounds and other trace gasses together with PM_(2.5)over urban Jinan in North China Plain during the winter.Markedly higher carbonyl concentrations(average:14.63±4.21 ppbv)were found during wintertime haze pollution,about one to three-times relative to those on nonhaze days,with slight difference in chemical composition except formaldehyde(HCHO).HCHO(3.68 ppbv),acetone(3.17 ppbv),and acetaldehyde(CH_3CHO)(2.83 ppbv)were the three most abundant species,accounting for~75% of the total carbonylson both haze and non-haze days.Results from observational-based model(OBM)with atmospheric oxidation capacity(AOC)indicated that AOC significantly increased with the increasing carbonyls during the winter haze events.Carbonyl photolysis have supplied key oxidants such as RO_(2) and HO_(2),and thereby enhancing the formation of fine particles and secondary organic aerosols,elucidating the observed haze-carbonyls inter-correlation.Diurnal variation with carbonyls exhibiting peak values at early-noon and night highlighted the combined contribution of both secondary formation and primary diesel-fuel sources.1-butene was further confirmed to be the major precursor for HCHO.This study confirms the great contribution of carbonyls to AOC,and also suggests that reducing the emissions of carbonyls would be an effective way to mitigate haze pollution in urban area of the NCP region.

关键词： Carbonyls Haze Observation-based model Source apportionment Atmospheric oxidation capacity

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Single-center Experience in the Diagnosis and Treatment of Hepatic Perivascular Epithelioid Cell Neoplasm

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Journal of Clinical and Translational Hepatology 2022年第1期10卷 72-79页

作者： Junjun Jia Jia Luo Cheng-gen pan Guomei Ge Meibao Feng Bei Zou Li Liu Shusen Zheng Jun Yu Division of Hepatobiliary and pancreatic Surgery Department of SurgeryThe First Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiangChina Key Laboratory of Organ Transplantation Research Center for Diagnosis and Treatment of Hepatobiliary DiseasesHangzhouZhejiangChina Department of Pathology The First Affiliated Hospi-talCollege of MedicineHangzhouZhejiangChina Department of Library The First Affiliated HospitalZhejiang Univer-sity School of MedicineHangzhouZhejiangChina

Background and Aims:Perivascular epithelioid cell neo-plasms(PEComas)are a rare type of mesenchymal neo-plasm and their preoperative diagnosis is challenging.In this study,we summarized the experience from a single medical center to study the examinations,clinical presen-tations,and pathological and histological characteristics of PEComas in the liver in order to optimize overall un-derstanding of the diagnosis and treatment of these neo-plasms.Methods:We conducted a retrospective analysis to investigate the clinical and pathological characteristics as well as imaging presentations of 75 patients diagnosed with hepatic PEComa in The First Affiliated Hospital of Zhe-jiang University between April 2010 and April 2020.Re-sults:Among the 75 patients,52 were women,and the median age was 48 years.Most patients had no specific symptoms,and two were admitted to the hospital for a second time owing to relapse.All patients underwent surgi-cal resection.Histologically,38 patients had classical angio-myolipoma(AML)and 37 had epithelioid AML.The PECo-mas were accompanied by positive immunohistochemical expression of HMB45,Melan-A,and smooth muscle actin.Follow-up data were obtained from 47 of the total 75 pa-tients,through October 2020.Two patients had metastasis after surgery.Conclusions:AML is the most common type of hepatic PEComa.There are no specific symptoms of he-patic PEComa,and serological examinations and imaging modalities for accurate preoperative diagnosis are lacking.Epithelioid AML should be considered a tumor of uncertain malignant potential;however,the prognosis of PEComa af-ter resection is promising.

关键词： Liver Perivascular epithelioid cell neoplasms Diagnosis Treatment

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Topsoil organic carbon mineralization and CO_2 evolution of three paddy soils from South China and the temperature dependence

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Journal of Environmental Sciences 2007年第3期19卷 319-326页

作者： ZHANG Xu-hui LI Lian-qing pan gen-xing Institute of Resources Ecosystem and Environment of Agriculture Nanjing Agricultural University Nanjing 210095 China.

Carbon mineralization and its response to climatic warming have been receiving global attention for the last decade. Although the virtual influence of temperature effect is still in great debate, little is known on the mineralization of organic carbon （SOC） of paddy soils of China under warming. SOC mineralization of three major types of China＇s paddy soils is studied through laboratory incubation for 114 d under soil moisture regime of 70% water holding capacity at 20℃ and 25℃ respectively. The carbon that mineralized as CO2 evolved was measured every day in the first 32 d and every two days in the following days. Carbon mineralized during the 114 d incubation ranged from 3.51 to 9.22 mg CO2-C/gC at 20℃ and from 4.24 to 11.35 mg CO2-C/gC at 25℃ respectively; and a mineralizable C pool in the range of 0.24 to 0.59 gC/kg, varying with different soils. The whole course of C mineralization in the 114 d incubation could be divided into three stages of varying rates, representing the three subpools of the total mineralizable C： very actively mineralized C at 1-23 d, actively tnineralized C at 24--74 d and a slowly mineralized pool with low and more or less stabilized C mineralization rate at 75-114 d. The calculated Q10 values ranged from 1.0 to 2.4, varying with the soil types and N status. Neither the total SOC pool nor the labile C pool could account for the total mineralization potential of the soils studied, despite a well correlation of labile C with the shortly and actively mineralized C, which were shown in sensitive response to soil warming. However, the portion of microbial C pool and the soil C/N ratio controlled the C mineralization and the temperature dependence. Therefore, C sequestration may not result in an increase of C mineralization proportionally. The relative control of C bioavailability and microbial metabolic activity on C mineralization with respect to stabilization of sequestered C in the paddy soils of China is to be further studied.

关键词： C mineralization carbon pool laboratory incubation paddy soil soil warming

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Sorption of pyrene on two paddy soils and their particle-size fractions

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Journal of Environmental Sciences 2005年第6期17卷 962-965页

作者： LI Jiu-hai pan gen-xing Institute of Resources Ecosystem and Environment of Agriculture Nanjing Agricultural University Nanjing 210095 China

In the present study, the sorption of pyrene on two kinds of bulk paddy soils, Gleyic Stagnic Anthrosols, and Ferric accumulic Stagnic Anthrosols as well as their particle-size fractions was investigated. The sorption isotherms fitted well with Freundlich equation. For both soils, the clay fraction（〈 2μm） and coarse sand fraction（2000-250μm） had higher sorption capacity than fine sand fraction（250-20 μm） and silt fraction（20-2 μm）. The IogKoc values obtained of each soil and its particle-size fractions were similar, proving that SOM content was a key factor affecting pyrene sorption. The Kd values showed a significant correlation with contents of dithionite-extractable Fe in both paddy soils and a good relationship with CEC in Gleyic Stagnic Anthrosols, indicating possible effects of surface properties of particle-size fractions on the sorption of pyrene.

关键词： paddy soil particle-size fraction PAHs sorption

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Behçet Disease with generalized Polymorphous Cutaneous Lesions:A Case Report

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International Journal of Dermatology and Venereology 2022年第3期5卷 175-177页

作者： Li-Wei Wang Ling-Ling Luo Wei Zhang Xue-Si Zeng pan-gen Cui Department of Dermatology Department of Pathology Hospital for Skin Diseases(Institute of Dermatology)Chinese Academy of Medical Sciences and Peking Union Medical CollegeNanjingJiangsu 210042China.

Introduction:Behçet disease is a chronic multisystem vasculitis disease,however generalized polymorphous cutaneous lesions are uncommon.Here,we reported a case of Behçet disease with multiple parts of the body and complex lesions,which may lead to misjudging in clinical diagnosis.Case report:A 69-year-old man presented with a seven-year history of recurrent generalized polymorphous mucocutaneous lesions(erythematous papules,nodules,ulcers,and necrosis)over his entire body,and the lesions had been painful for the past three years.Based on the past medical history,clinical presentation,histological examination excluded other diseases,and the 2014-amended International Criteria for Behget disease,the patient had a score of 6 points and was diagnosed as Behçet disease.Discussion:The common clinical feature in patients with Behçet syndrome is the presence of recurrent and usually painful mucocutaneous ulcers.Other clinical manifestations of this disorder are more variable among different patients.A diagnosis of generalized polymorphous cutaneous lesions should remain on the list of differential diagnosis of Behçet disease after excluding other diseases.Conclusion:The mucocutaneous lesions of Behçet disease are often preceded by other manifestations,and timely diagnosis may benefit early treatment and prognosis.

关键词： Behçet disease cutaneous lesions generalized lesions poloymorphous

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Distribution of Cu and Pb in particle size fractions of urban soils from different city zones of Nanjing, China

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Journal of Environmental Sciences 2006年第3期18卷 482-487页

作者： WANG Huan-hua LI Lian-qing WU Xin-min pan gen-xing Institute of Resources Ecosystem and Environment of Agriculture Nanjing Agricultural University Nanjing 210095 China Chizhou Junior Teachers College Chizhou 247100 China

Soil samples from 4 defined city zones of Nanjing were randomly collected at 0-5 cm and 5-20 cm intervals and size fractions of soil particles were separated from undisturbed bulk soils by low energy dispersion procedure. The total contents of Cu and Pb in the different particle size fractions of the urban soils were analyzed by HNO3-HF-HClO4 digestion and flame atomic absorption spectrophotometer determination. The total content of Cu and Pb in soil particle size fractions varied with their size and with city zones as well. Both the content and variation with the size fractions of Pb was bigger than of Cu supporting our previous finding that there was Pb pollution to different degrees in the urban soils although the two elements were generally enriched in clay-sized fraction. Contaminated Pb tended to be preferentially enriched in the size fraction of 2000-250 μm and clay-sized fraction. While the size fractions of the soils from newly developed and preserved area contained smaller amount of Cu and Pb, the partitioning of them in coarse and fine particle size fractions were insignificant compared to that from inner residence and commercial area. The very high Pb level over 150 mg/kg of the fine particle fractions from the soils of the inner city could be a cause of high blood Pb level reported of children from the city as acute exposure to Pb of fine particles of the urban soil might occur by soil ingestion and inhalation by young children. Thus, much attention should be paid to the partitioning of toxic metals in fine soil particles of the urban soils and countermeasures against high health risk of Pb exposure by soil ingestion and dust inhalation should be practiced against the health problem of blood Pb for young children from the cities.

关键词： urban soil city zone soil contamination particle size fraction heavy metals lead health risk

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Construction of tissue-engineered heart valves by using decellularized scaffolds and endothelial progenitor cells

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Chinese Medical Journal 2007年第8期120卷 696-702页

作者： FANG Ning-tao XIE Shang-zhe WANG Song-mei GAO Hong-yang WU Chun-gen pan Luan-feng Laboratory of Molecular Biology Shanghai Medical College Fudan University Shanghai 200032 China

Background Tissue-engineered heart valves have the potential to overcome the limitations of present heart valve replacements. This study was designed to develop a tissue engineering heart valve by using human umbilical cord blood-derived endothelial progenitor cells （EPCs） and decellularized valve scaffolds. Methods Decellularized valve scaffolds were prepared from fresh porcine heart valves. EPCs were isolated from fresh human umbilical cord blood by density gradient centrifugation, cultured for 3 weeks in EGM-2-MV medium, by which time the resultant cell population became endothelial in nature, as assessed by immunofluorescent staining. EPC-derived endothelial cells were seeded onto the decellularized scaffold at 3 × 10^6 cells/cm^2 and cultured under static conditions for 7 days. Proliferation of the seeded cells on the scaffolds was detected using the MTT assay. Tissue-engineered heart valves were analyzed by HE staining, immunofluorescent staining and scanning electron microscopy. The anti-thrombogenic function of the endothelium on the engineered heart valves was evaluated by platelet adhesion experiments and reverse transcription-polymerase chain reaction （RT-PCR） analysis for the expression of endothelial nitric oxide synthase （eNOS） and tissue-type plasminogen activator （t-PA）.Results EPC-derived endothelial cells showed a histolytic cobblestone morphology, expressed specific markers of the endothelial cell lineage including von Willebrand factor （vWF） and CD31, bound a human endothelial cell-specific lectin, Ulex Europaeus agglutinin-1 （UEA-1）, and took up Dil-labeled low density lipoprotein （Dil-Ac-LDL）. After seeding on the decellularized scaffold, the cells showed excellent metabolic activity and proliferation. The cells formed confluent endothelial monolayers atop the decellularized matrix, as assessed by HE staining and immunostaining for vWF and CD31. Scanning electron microscopy demonstrated the occurrence of tight junctions between cells forming the confluent monolayer. Platelets adhesion ex

关键词： endothelial progenitor cell tissue-engineered heart valve decellularized matrix platelet adhesion

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