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雌激素受体Esr1诱导红耳龟早期卵巢分化

生物工程学报 2024年第7期40卷 2308-2321页

作者：郭金洪杨洪哲时翔胡颖王圣云汪思凡孙伟葛楚天浙江万里学院动物性别与发育重点研究所浙江宁波315100 浙江万里学院生物与环境学院浙江宁波315100 中国海洋大学海洋生命学院山东青岛266000

为探究3种雌激素受体(estrogen receptor) Esr1、Esr2和Gper1在红耳龟早期胚胎性腺分化中的作用,本研究在分析受体基因表达特征的基础上,通过向性腺分化启动前的产雄温度(male-producing temperature,MPT)龟胚分别注射Esr1、Esr2和Gper... 详细信息

为探究3种雌激素受体(estrogen receptor) Esr1、Esr2和Gper1在红耳龟早期胚胎性腺分化中的作用,本研究在分析受体基因表达特征的基础上,通过向性腺分化启动前的产雄温度(male-producing temperature,MPT)龟胚分别注射Esr1、Esr2和Gper1激动剂PPT、WAY 200070和G-1,从性腺形态结构、生殖细胞分布模式、性别分化关键基因和蛋白表达分布方面对处理后的胚胎性腺进行了性逆转分析。表达分析结果显示,esr1在性别分化关键时期性腺中的表达量显著高于esr2和gper1 (表达极低),且呈现产雌温度(female-producing temperature,FPT)性腺高表达。功能验证实验显示,PPT处理后的MPT性腺形态结构明显雌性化,生殖细胞呈现雌性分布模式;雄性分化关键基因dmrt1、amh和sox9 m RNA表达明显下降,雌性分化关键基因foxl2和cyp19a1 m RNA表达则显著上升;Amh和Sox9蛋白表达的荧光信号几乎消失,Foxl2和Arom蛋白被激活出现大量表达,表明性腺由雄性逆转为雌性(性逆转率:70.27%)。而WAY 200070和G-1处理后的MPT性腺仍分化为睾丸,雌雄基因和蛋白的表达及分布与雄性性腺类似。结果表明在红耳龟中,单独激活Esr1能够充分启动早期性腺的雌性分化过程,提示雌激素可能通过其受体1 (Esr1)诱导早期卵巢分化。本研究为进一步解析雌激素在龟性别决定和分化中的调控机制提供了参考。

关键词：雌激素受体性腺分化雌激素性逆转红耳龟

Intelligent extraction of road cracks based on vehicle laser point cloud and panoramic sequence images

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Journal of Road Engineering 2024年第1期4卷 69-79页

作者： Ming Guo Li Zhu Ming Huang Jie Ji Xian Ren Yaxuan Wei chutian Gao School of geomatics and Urban Spatial Informatics Beijing University of Civil Engineering and ArchitectureBeijing 102616China Engineering Research Centre of Representative Building and Architectural Heritage Database Ministry of EducationBeijing 100044China Key Laboratory of Modern Urban Surveying and Mapping National Administration of SurveyingBeijing 102616China School of Civil and Transportation Engineering Beijing University of Civil Engineering and ArchitectureBeijing 102616China

In light of the limited efficacy of conventional methods for identifying pavement cracks and the absence of comprehensive depth and location data in two-dimensional photographs,this study presents an intelligent strategy for extracting road *** methodology involves the integration of laser point cloud data obtained from a vehicle-mounted system and a panoramic sequence of *** study employs a vehicle-mounted LiDAR measurement system to acquire laser point cloud and panoramic sequence image data simultaneously.A convolutional neural network is utilized to extract cracks from the panoramic sequence *** extracted sequence image is then aligned with the laser point cloud,enabling the assignment of RGB information to the vehicle-mounted three dimensional(3D)point cloud and location information to the two dimensional(2D)panoramic ***,a threshold value is set based on the crack elevation change to extract the aligned roadway point *** three-dimensional data pertaining to the cracks can be *** experimental findings demonstrate that the use of convolutional neural networks has yielded noteworthy outcomes in the extraction of road *** utilization of point cloud and image alignment techniques enables the extraction of precise location data pertaining to road *** approach exhibits superior accuracy when compared to conventional ***,it facilitates rapid and accurate identification and localization of road cracks,thereby playing a crucial role in ensuring road maintenance and traffic ***,this technique finds extensive application in the domains of intelligent transportation and urbanization *** technology exhibits significant promise for use in the domains of intelligent transportation and city development.

关键词： Road crack extraction Vehicle laser point cloud Panoramic sequence images Convolutional neural network

Controllability of Semilinear Integrodifferential Degenerate Sobolev Equations

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Journal of Systems Science & Complexity 2024年第5期37卷 1923-1936页

作者： ge Zhaoqiang School of Mathematics and Statistics Xi’an Jiaotong University

In this paper, the approximate controllability of semilinear integrodifferential degenerate Sobolev equations with nonlocal conditions is investigated in the sense of integral solution in Hilbert spaces. Some sufficient and necessary conditions are obtained. Firstly, the existence and uniqueness of integral solutions of semilinear integrodifferential degenerate Sobolev equations with nonlocal conditions are considered by ge-evolution operator theory and Sadovskii's fixed point theorem, the existence and uniqueness theorem of solutions is given. Secondly, the approximate controllability of semilinear integrodifferential degenerate Sobolev equations with nonlocal conditions is studied in the sense of integral solution. The criterion for approximate controllability is provided. The obtained results have important theoretical and practical value for the study of controllability of semilinear integrodifferential degenerate Sobolev equations with nonlocal conditions.

关键词： Approximate controllability ge-evolution operator integral solution Sadovskii’s fixed point theorem semilinear integrodifferential degenerate Sobolev equations

On the use of deep learning for phase recovery

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Light(Science & Applications) 2024年第2期13卷 190-235页

作者： Kaiqiang Wang Li Song chutian Wang Zhenbo Ren Guangyuan Zhao Jiazhen Dou Jianglei Di george Barbastathis Renjie Zhou Jianlin Zhao Edmund Y.Lam Department of Electrical and Electronic Engineering The University of Hong KongHong Kong SARChina School of Physical Science and Technology Northwestern Polytechnical UniversityXi’anChina Department of Biomedical Engineering The Chinese University of Hong KongHong Kong SARChina School of Information Engineering Guangdong University of TechnologyGuangzhouChina Department of Mechanical Engineering Massachusetts Institute of TechnologyCambridgeMAUSA

Phase recovery(PR)refers to calculating the phase of the light field from its intensity *** exemplified from quantitative phase imaging and coherent diffraction imaging to adaptive optics,PR is essential for reconstructing the refractive index distribution or topography of an object and correcting the aberration of an imaging *** recent years,deep learning(DL),often implemented through deep neural networks,has provided unprecedented support for computational imaging,leading to more efficient solutions for various PR *** this review,we first briefly introduce conventional methods for ***,we review how DL provides support for PR from the following three stages,namely,pre-processing,in-processing,and *** also review how DL is used in phase image ***,we summarize the work in DL for PR and provide an outlook on how to better use DL to improve the reliability and efficiency of ***,we present a live-updating resource(https://***/kqwang/phase-recovery)for readers to learn more about PR.

关键词： networks phase optics

Polymeric piezoelectric accelerometers with high sensitivity,broad bandwidth,and low noise density for organic electronics and wearable microsystems

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Microsystems & Nanoengineering 2024年第3期10卷 63-74页

作者： Chang ge Edmond Cretu The Department of Electrical and Computer Engineering The University of British ColumbiaVancouverBCCanada

Piezoelectric accelerometers excel in vibration *** the emerging trend of fully organic electronic microsystems,polymeric piezoelectric accelerometers can be used as vital front-end components to capture dynamic signals,such as vocal vibrations in wearable speaking assistants for those with speaking ***,high-performance polymeric piezoelectric accelerometers suitable for such applications are *** organic compounds such as PVDF have inferior properties to their inorganic counterparts such as ***,most existing polymeric piezoelectric accelerometers have very unbalanced performance *** often sacrifice resonance frequency and bandwidth for a flat-band sensitivity comparable to those of PZT-based accelerometers,leading to increased noise density and limited application *** this study,a new polymeric piezoelectric accelerometer design to overcome the material limitations of PVDF is *** new design aims to simultaneously achieve high sensitivity,broad bandwidth,and low *** samples were manufactured and characterized,demonstrating an average sensitivity of 29.45 pC/g within a±10 g input range,a 5%flat band of 160 Hz,and an in-band noise density of 1.4μg/√*** results surpass those of many PZT-based piezoelectric accelerometers,showing the feasibility of achieving comprehensively high performance in polymeric piezoelectric accelerometers to increase their potential in novel applications such as organic microsystems.

关键词： piezoelectric polymeric overcome

Meridional deflection of global eddy propagation derived from tandem altimetry: Mechanism and implication

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Science China Earth Sciences 2024年第3期67卷 811-825页

作者： ge CHEN Xiaoyan CHEN Frontiers Science Center for Deep Ocean Multispheres and Earth System School of Marine TechnologyOcean University of ChinaQindao 266100China Laboratory for Regional Oceanography and Numerical Modeling Laoshan LaboratoryQindao 266237China

A common view on meridional deflection of eddy propagation is poleward for cyclones and equatorward for anticyclones. This widely accepted polarity-sensitive pattern has been increasingly undermined given that it is systematically violated on both zonal and regional scales as recently reported. Here, we approach the problem by exploring these violations in terms of eddy effects of spatiotemporal geostrophy, thermal fading, and flow dragging, yielding the following main conclusions.(1) The polarity-dependent deflection discrepancy is a pseudo feature resulting from a biased fit to the normalized eddy tracks which are spatially asymmetric(though azimuthally symmetric) between the two signs;Instead, the primary pattern of eddy deflection appears to be geographically correlated and polarity independent.(2) geostrophic adjustment plays a critical role in defining the meridional movement of an eddy. Specifically, a consistent poleward(equatorward) trajectory bending of spatially(temporally) ageostrophic eddies is observed irrespective of their polarity.(3) The mechanism of eddy deflection is also determined by its zonal propagation direction. Westward propagating eddies are substantially affected by thermal fading, while opposite ones by flow dragging. A realistic and comprehensive understanding of eddy deflection mechanisms serves as a significant contribution to the prediction of eddy trajectory, and the protection of ocean environment.

关键词： Mesoscale eddies Eddy propagation Eddy meridional deflection Satellite observation geostrophic adjustment

Solid Desiccant Heat Pump Fresh Air Unit using Composite Silica gel

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Journal of Thermal Science 2024年第4期33卷 1286-1300页

作者： YANG Tianyu GUO Defang ge Tianshu Institute of Refrigeration and Cryogenics Shanghai Jiao Tong UniversityKey Laboratory of Power Mechanical EngineeringMOE ChinaShanghai200240China Qingdao Haier A/C Electronic Co. LtdQingdao266101China

The introduction of fresh air into the indoor space leads to a significant increase in cooling or heating *** desiccant heat pump fresh air unit which can handle the latent and sensible load of fresh air efficiently have been proposed *** improve the performance of the solid desiccant heat pump fresh air unit in the fresh air handling process,in this paper,the application of composite silica gel in a heat pump fresh air unit was *** comparison between silica gel coating(SGC)and composite silica gel coating(CSGC)shows that the adsorption rate and water uptake capacity of CSGC are more than two times higher than those of *** experimental setup for the solid desiccant heat pump fresh air unit was *** performance of SGC and CSGC was tested in the setup *** show that under summer conditions,compared with the solid desiccant heat pump fresh air unit using silica gel(SGFU),the average moisture removal and COP of the one using composite silica gel(CSGFU)increased by 15%and 30%,*** winter conditions,compared with SGFU,the average humidification and COP of CSGFU increased by 42%and 17%.With optimal operation conditions of 3 min switchover time and 40 r/s compressor frequency,the COP of CSGFU under summer conditions can reach *** also show that the CSGFU and SGFU have higher COP and dehumidification rate under higher outdoor temperature and humidity ratio.

关键词： fresh air system desiccant coated heat exchanger humidification dehumidification experimental comparison

Theoretical study on the stability of nanobubbles and its verification in molecular dynamics simulation

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Particuology 2024年第4期87卷 99-105页

作者： Sheng Mi Chaofeng Hou Wei ge School of Chemical Engineering and Technology Tianjin UniversityTianjin300072China State Key Laboratory of Multiphase Complex Systems Institute of Process EngineeringChinese Academy of SciencesBeijing.100190China School of Chemical Engineering University of Chinese Academy of SciencesBeijing100049China

The stability of vapor nanobubbles in bulk liquid was investigated theoretically and the critical bubble size was derived from macroscale thermodynamic equations,below which the system destabilizes with sharp drop in *** critical size was quantitatively verified in molecular dynamic simulation using the Lennard-Jones model of argon,where stronger attraction between the molecules at lower density is found to contribute most to the drop of system pressure and,as the Laplace pressure on the curved bubble interface fails to balance the pressure difference across the interface,the bubbles become *** theoretical model could be extended to other systems where reliable equations of state and interfacial tension are available.

关键词： Nanobubble Stability Phase diagram Critical size

Soft coarse-grained particle model for particle-fluid systems

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Particuology 2024年第1期84卷 178-193页

作者： Yanhao Ye Ji Xu Wei ge State Key Laboratory of Multiphase Complex Systems Institute of Process EngineeringChinese Academy of SciencesBeijing100190China School of Chemical Engineering University of Chinese Academy of SciencesBeijing100049China Innovation Academy for Green Manufacture Chinese Academy of SciencesBeijing100190China

By modeling a group of neighboring real particles as a single coarse-grained particle(CGP),discrete particle method(DPM)is now capable of simulating industrial-scale particle-fluid ***,a systematic approach to determine the CGP properties and develop their interaction models is still lacking,which casts uncertainty on the predictivity of the *** this study,collisions between predefined particle groups are analyzed to construct kernel functions for modeling the CGPs and then the model parameters are determined by equating the statistical properties of the CGPs and the real particles in the physical process *** approach is implemented for homogeneous cooling of granular gas,then demonstrated effective in simulating experimental fluidized beds.

关键词： Coarse-grain(CG) Discrete particle method(DPM) Fluidized bed Multi-scale simulation