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Highly-efficient quantitative fluorescence resonance energy transfer measurements based on deep learning

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Journal of Innovative Optical Health Sciences 2020年第6期3卷 23-35页

作者： lin ge Fei Liu Jianwen Luo Department of Biomedical Engineering School of Medicine Tsinghua UniversityBeijing100084China Beijing Advanced Information&Industrial Technology Research Institute Beijing Information Science&Technology University Beijing 100192China

Intensity-based quantitative fluorescence resonance energy transfer(FRET)is a technique to measure the distance of molecules in scale of a few nanometers which is far beyond optical diffraction *** widely used technique needs complicated experimental process and manual image analyses to obtain precise results,which take a long time and restrict the application of quantitative FRET especially in living *** this paper,a simplified and automatic quanti-tative FRET(saqFRET)method with high efficiency is *** saqFRET,photo-activatable acceptor PA-mCherry and optimized excitation wavelength of donor enhanced green fluorescent protein(EGFP)are used to simplify FRET crosstalk *** manual image analyses are time consuming when the dataset is *** proposed automatic image analyses based on deep learning can analyze 100 samples within 30 s and demonstrate the same precision as manual image analyses.

关键词： Resonance energy transfer fluorescence living cells photoactivatable deep network

Steady-state performance analysis of HOS-based blind adaptive multiuser detection

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Science in China(Series F) 2009年第7期52卷 1243-1249页

作者： ZHENG lin QIU HongBing ge Wei lin JiMing Guilin University of Electronic Technology Guilin 541004 China

The blind adaptive multiuser detections based on higher-order statistics （HOS） can obtain higher steady-state decorrelating performance than the conventional linear algorithm under the high SNR con- dition. However, the closed-form analysis for this steady-state performance is scarce due to the com- plication of analyzing the nonlinear updates of the adaptive algorithm. An analysis approach based on ordinary differential equation （ODE） method is proposed to get the closed-form excess mean-square error （EMSE） expression of the HOS-based multiuser detections. The simulation and the comparison verify the results of the analysis.

关键词： ordinary differential equation blind multiuser detection higher-order statistics multi-access interference CDMA

Theoretical modelling of liquid–liquid phase separation: from particle-based to field-based simulation

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Biophysics Reports 2022年第2期8卷 55-67页

作者： lin-ge Li Zhonghuai Hou Hefei National Laboratory for Physical Sciences at the Microscale & Department of Chemical Physics University of Science and Technology of China

Liquid–liquid phase separation(LLPS) has proved to be ubiquitous in living cells, forming membraneless organelles(MLOs) and dynamic condensations essential in physiological processes. However,some underlying mechanisms remain challenging to unravel experimentally, making theoretical modeling an indispensable aspect. Here we present a protocol for understanding LLPS from fundamental physics to detailed modeling procedures. The protocol involves a comprehensive physical picture on selecting suitable theoretical approaches, as well as how and what to interpret and resolve from the results. On the particle-based level, we elaborate on coarse-grained simulation procedures from building up models, identifying crucial interactions to running simulations to obtain phase diagrams and other concerned properties. We also outline field-based theories which give the system's density profile to determine phase diagrams and provide dynamic properties by studying the time evolution of density field, enabling us to characterize LLPS systems with larger time and length scales and to further include other nonequilibrium factors such as chemical reactions.

关键词： Liquid–liquid phase separation Theoretical modelling Coarse-grained simulation

Effect of transition metal oxides doping on Ce_(0.9)Sm_(0.05)Nd_(0.05)O_(1.95) solid electrolyte materials

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Journal of Advanced Ceramics 2012年第2期1卷 150-156页

作者： Ming ZHOU lin ge Han CHEN Lucun GUO College of Materials Science and Engineering Nanjing University of TechnologyNo.5 Xinmofan RoadNanjing210009China

The effect of transition metal oxides(TMOs)Co_(O1.5),Fe_(O1.5)and MnO_(2)addition on Ce_(0.9)Sm_(0.05)Nd_(0.05)O_(1.95)was *** crystal structures,microstructures,thermal expansion and electrical properties were characterized by X-ray diffraction(XRD),scanning electron microscopy(SEM),dilatometer and AC impedance spectroscopy,*** results show that the TMOs addition remarkably promotes the densification of Ce_(0.9)Sm_(0.05)Nd_(0.05)O_(1.95)and reduces the sintering temperature by~150℃.The samples with TMOs addition sintered at 1450℃exhibit higher conductivities than those sintered at 1500℃.The maximum conductivity of 0.040 ***^(-1)at 700℃was achieved with 1 mol%FeO1.5 doping when sintered at 1450℃.In addition,the thermal expansion was linear for all the *** doping of TMOs does not appreciably change thermal expansion coefficient.

关键词： transition metal oxides microstructure thermal expansion ionic conductivity

Periodical Oscillations of Supercurrent Fluctuation in Mesoscopic Josephson Junctions in Quantized Electromagnetic Fields

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Chinese Physics Letters 1996年第5期13卷 359-361页

作者： KUANG Le-man WANG Yi-wen ge Mo-lin Theoretical Physics Division Nankai Instilute of MathematicsTianjin 300071 Department of Physics Hu'nan Normal UriiversityChangsha 410081

Supercurrent fluctuation of a mesoscopic Josephson jutiction(MJJ)in the presence of a de static voltage and a time-varying quantized electromagnetic field(QEMF)is *** is shown thm the fluctuation of supercurrent in the MJJ exhibits periodical oscillations with a periodicity shich depends upon the rate of the classical static external voltage and the frequency of the QEMF.

关键词： Josephson Electromagnetic Periodical

LncRNA C9orf139 can regulate the growth of pancreatic cancer by mediating the miR-663a/Sox12 axis

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World Journal of Gastrointestinal Oncology 2020年第11期12卷 1272-1287页

作者： Jin-Nian ge Di Yan Chun-lin ge Min-Jie Wei Department of general Surgery The First Affiliated Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina Intensive Care Unit The Central Affiliated Hospital of Shenyang Medical CollegeShenyang 110024Liaoning ProvinceChina Department of Pharmacology School of PharmacyChina Medical UniversityShenyang 110122Liaoning ProvinceChina

BACKGROUND Recent studies have proved the important role of many oncogenic long noncoding RNAs(lncRNAs) in the progression of pancreatic cancer, but little is known about the mechanisms of tumor suppression in pancreatic *** To evaluate the function of tumor suppressor lncRNA C9orf139 in pancreatic cancer progression and to study the underlying *** We assigned 54 patients with pancreatic ductal adenocarcinoma treated at our hospital to the patient group and 30 normal subjects undergoing physical examination to the control group. RT-qPCR was used to measure the relative expression of C9orf139 in the tissue and serum of patients, in an attempt to investigate the prognostic value of C9orf139 in pancreatic cancer patients. The luciferase reporter gene assay was performed to determine the interaction between C9orf139 and miR-663 a. The biological function of C9orf139 was assessed by in vitro assays and in vivo subcutaneous tumor formation tests in animal models. To figure out the molecular mechanism of C9orf139 to act on miR-663 a/Sox12, RNA pull-down, Western blot assay, RNA immunoprecipitation assay, and co-immunoprecipitation assay were *** C9orf139 level significantly increased in the tissue and serum of patients, which had clinical diagnostic value for pancreatic cancer. Patients with high C9orf139 expression had a higher risk of progressing to stage Ⅲ + Ⅳ, lymph node metastasis, and poor differentiation. Cox regression analysis suggested that C9orf139, tumor-node-metastasis stage, and lymph node metastasis were independent prognostic factors in patients. The underlying mechanism of C9orf139 was that it promoted the growth of pancreatic cancer cells by modulating the miR-663 a/Sox12 *** C9orf139 is highly expressed in pancreatic cancer, qualified to be used as a potential diagnostic and prognostic marker for pancreatic cancer. Its promotion of pancreatic cancer cell growth is achieved by mediating the miR-663 a/Sox12 ax

关键词： C9orf139 miR-663a Sox12 Pancreatic cancer Prognosis Tumor formation in nude mice

geometric Phase in Optical Fiber

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Chinese Physics Letters 1999年第5期16卷 316-317页

作者： ZHOU Yi WU Zhen-hua ge Mo-lin Center for Advanced Study Tsinghua UniversityBeijing 100084

An additional geometric phase viewed as Berry's phase is found in helically coiled monomode optical fiber when linear birefiingences are induced by *** phase appears when the optical axis and normal vector do not coin... 详细信息

关键词： material. optical fiber

Numerical simulation of rock deformation during the mineralization of the Xiangshan uranium deposit, Jiangxi province, south China

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Journal of China University of Mining and Technology 2008年第3期18卷 342-346页

作者： ZHOU Ye lin ge YANG Li-qiang Beijing general Research Institute of Mining ＆ Metallurgy Beijing 100044 China State Key Laboratory of geological Process and Mineral Resources China University of Geosciences Beijing 100083 China Key Laboratory of Marginal Sea geology Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640 China

The Xiangshan uranium deposit in Jiangxi province is one of the most important uranium deposits in China. The aim of our study is to obtain a better understanding of rock deformation and dilation associated with mineralization, to predict the most favorable locations of mineralization, and to assist with future mineral exploration in this deposit. On the basis of geological and structural data from previous studies, we have constructed a coupled deformation and fluid flow numerical model and simulated the faulting deformation and major mechanical factors controlling mineralization in the deposit. Particular attention has been paid to variations in regional stress, distributions of shear strain, volumetric strain and pore pressure. The relationship between the struc-tural/faulting movement and mineralization is obtained through analyzing the deformation state of fault zones. The results suggest that the mineralization is related to volumetric strain, shear strain and pore pressures. The locations displaying all these factors rep-resent the most favorable sites for mineralization. These model results are important for guiding the exploration of new uranium deposits in Xiangshan.

关键词： numerical simulation Xiangshan rock deformation volumetric strain shear strain pore pressure

Numerical Simulations of Structural Deformation and Fluid Flow in Xiangshan Deposit

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Journal of China University of Mining and Technology 2006年第4期16卷 404-408页

作者： ZHOU Ye lin ge GONG Fa-xiong LIU Shi-lin Key Laboratory of Marginal Sea geology Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou Guangdong 510640 China State Key Laboratory of geological Process and Mineral Resources China University of Geosciences Beijing 100083 China Graduate School of Chinese Academy of Sciervces Beijing 100049 China

The Xiangshan deposit in Jiangxi province is one of the most important uranium deposits in China. The aim of this study is to achieve a better understanding of mineralization in the Xiangshan deposit through numerical simulation. In order to find the most favorable locations of mineralization and to help further mineral exploration, a coupling deforma- tion and fluid flow model has been established to describe the mineralization process. In this model, the simulation re- constructs the strata deformations under fields of compressive stress and thrust structure on the hanging wall of the Zou-Shi fault. Compared with practical information, the simulation results are consistent with the No. 51 exploration section of the western Xiangshan. In addition, on the basis of geological information provided by previous investigators, the model simulates the flow process of fluids under compressive stress fields. The result suggests that many tensional areas are formed, which can help the fluid flowing upward from deeper parts. The fluid is easy to concentrate on the breccia fractured zone between two volcanic layers, especially on the intersection parts with faults, resulting in the for- mation of favourable locations of mineralization. In addition, the model is significant in guiding the exploration of ura- nium deposits in the western Xiangshan and provides clues for further exploration of deposits.

关键词： numerical simulation Xiangshan structural deformation fluid flow