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Antifibrotic effect of heparin on liver fibrosis model in rats

World Journal of Gastrointestinal Pharmacology and Therapeutics 2012年第6期3卷 86-92页

作者： Binita shah gaurang shah Department of Pharmacology K B Institute of Pharmaceutical Education and Research Gandhinagar 382023 Gujarat India

AIM: To evaluate the effect of chronic thrombin inhibition by heparin on experimentally induced chronic liver injury (liver fibrosis) in rats. METHODS: Chronic liver injury (liver fibrosis) was induced in Wistar rats by oral administration of carbon tetrachloride (CCl 4 ) for 7 wk, an animal model with persistent severe hepatic fibrosis. Intravenous administration of the thrombin antagonist (heparin) started 1 wk after the start of CCl 4 intoxication for 6 wk. After completion of treatment (7 wk), markers of hepatic dysfunction were measured and changes evaluated histopathologically. RESULTS: Higher serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), total, direct and indirect bilirubin levels, as well as lower fibrinogen levels, were found in CCl 4 intoxicated rats. Heparin, silymarin and combination of drug (heparin and silymarin) treatment for 6 wk prevented a rise in SGOT, SGPT, ALP, total, direct and indirect bilirubin levels and improved fibrinogen levels. Deterioration in hepatic function determined by the fibrosis area was retarded, as evident from hepatic histopathology. Total protein levels were not changed in all ***: Heparin, a thrombin antagonist, preserved hepatic function and reduced severity of hepatic dysfunction/fibrogenesis. Combination of heparin and silymarin produced additional benefits on liver fibrosis.

关键词： Hepatic dysfunction Liver fibrosis Carbon tetrachloride Heparin Thrombin antagonist

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Determination of mycophenolic acid in human plasma by ultra performance liquid chromatography tandem mass spectrometry

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Journal of Pharmaceutical Analysis 2014年第3期4卷 205-216页

作者： Vivek Upadhyay Vikas Trivedi gaurang shah Manish Yadav Pranav S.Shrivastav Chemistry Department Kadi Sarva VishwavidyalayaGandhinagar 382015India Department of Chemistry School of SciencesGujarat University

A simple, sensitive and high throughput ultra performance liquid chromatography tandem mass spectrometry method has been developed for the determination of mycophenolic acid in human plasma. The method involved simple protein precipitation of MPA along with its deuterated analog as an internal standard （IS） from 50 mL of human plasma. The chromatographic analysis was done on Acquity UPLC C18 （100mm×2.1mm,1.7μm） column under isocratic conditions using acetonitrile and 10 mM ammonium formate, pH 3.00 （75：25, v/v） as the mobile phase. A triple quadrupole mass spectrometer operating in the positive ionization mode was used for quantitation. In-source conversion of mycophenolic glucuronide metabolite to the parent drug was selectively controlled by suitable optimization of cone voltage, cone gas flow and desolvation temperature. The method was validated over a wide concentration range of 15-15000 ng/mL. The mean extraction recovery for the analyte and IS was 〉95%. Matrix effect expressed as matrix factors ranged from 0.97 to 1.02. The method was successfully applied to support a bioequivalence study of 500 mg mycophenolate mofetil tablet in 72 healthy subjects.

关键词： Mycophenolic acid Mycophenolic acid-d3 UPLC-MS/MS In-source conversion Incurred sample reanalysis

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Spectroscopic Investigation and Characterizations of PAM/PEO Blends Films

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Soft 2015年第2期4卷 9-24页

作者： gaurang Patel Mundan B. Sureshkumar Purvi Patel Department of Physics Faculty of Science The M.S. University of Baroda Vadodara India Science and Humanities Department Dr. S & S S Ghandhy College Surat India

Polymer blends have been obtained in the form of dimensionally stable and free standing films and their properties were characterized by different techniques. FTIR analysis and Raman spectroscopic analysis cleared the hydrogen bonding intermolecular interaction between –CONH2 groups in Poly Acrylamide (PAM) and C-O-C and –CH2OH group in Poly Ethylene Oxide (PEO). From Differential Scanning Calorimeter (DSC) the study shows that crystallinity is increasing with PEO wt%. From polymer interaction parameter we also show that the polymer blend is miscible. Thermal stability of films is investigated by Thermo Gravimetric Analysis (TGA) and derivative Thermo Gravimetric Analysis (DrTG). From UV-Vis absorption spectra, absorption band edge, direct/ indirect band gap and optical activation energy have been calculated.