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The ruminal bacterial community in lactating dairy cows has limited variation on a day-to-day basis

Journal of Animal Science and Biotechnology 2019年第4期10卷 1108-1112页

作者： Joseph H.Skarlupka Maria E.Kamenetsky Kelsea A.Jewell garret suen Department of Bacteriology University of Wisconsin-Madison5159 MSB1550 Linden DriveMadisonWI 53706USA Department of Population Health Sciences University of Wisconsin-MadisonMadison 53726USA College of Agricultural and Life Sciences Statistical Consulting Lab University of Wisconsin-MadisonMadison 53706USA Department of Biology Centralia CollegeCentraliaWA 98531USA.

Dairy cows rely on a complex ruminal microbiota to digest their host-indigestible feed. Our ability to characterize this microbiota has advanced significantly due to developments in next-generation sequencing. However, efforts to sample the rumen, which typical y involves removing digesta directly from the rumen via a cannula, intubation, or rumenocentesis, is costly and labor intensive. As a result, the majority of studies characterizing the rumen microbiota are conducted on samples col ected at a single time point. Currently, it is unknown whether there is significant day-to-day variation in the rumen microbiota, a factor that could strongly influence conclusion drawn from studies that sample at a single time point. To address this, we examined day-to-day changes in the ruminal microbiota of lactating dairy cows using next-generation sequencing to determine if single-day sampling is representative of sampling across 3 consecutive days. We sequenced single-day solid and liquid fractions of ruminal digesta col ected over 3 consecutive days from 12 cannulated dairy cows during the early, middle, and late stages of a single lactation cycle using the V4 region of the bacterial 16 S r RNA gene. We then generated 97% similarity operational taxonomic units(OTUs) from these sequences and showed that any of the individual samples from a given 3-day sampling period is equivalent to the mean OTUs determined from the combined 3-d data set. This finding was consistent for both solid and liquid fractions of the rumen,and we thus conclude that there is limited day-to-day variability in the rumen microbiota.

关键词： Bacterial community Next-generation sequencing Rumen microbiota

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Assessing the microbiota of recycled bedding sand on a Wisconsin dairy farm

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Journal of Animal Science and Biotechnology 2022年第2期13卷 601-612页

作者： Hannah E.Pilch Andrew J.Steinberger Donald C.Sockett Nicole Aulik garret suen Charles J.Czuprynski Department of Pathobiological Sciences School of Veterinary MedicineUniversity of Wisconsin-MadisonMadison 53706USA Department of Bacteriology University of Wisconsin-MadisonMadison 53706USA Microbiology Doctoral Training Program University of Wisconsin-MadisonMadison 53706USA Wisconsin Veterinary Diagnostic Laboratory School of Veterinary MedicineUniversity of Wisconsin-MadisonMadison 53706USA

Background:Sand is often considered the preferred bedding material for dairy cows as it is thought to have lower bacterial counts than organic bedding materials and cows bedded on sand experience fewer cases of lameness and *** can also be efficiently recycled and reused,making it ***,some studies have suggested that the residual organic material present in recycled sand can serve as a reservoir for commensal and pathogenic bacteria,although no studies have yet characterized the total bacterial community *** we sought to characterize the bacterial community composition of a Wisconsin dairy farm bedding sand recycling system and its dynamics across several stages of the recycling process during both summer and winter using 16S rRNA gene amplicon ***:Bacterial community compositions of the sand recycling system differed by both seasons and *** samples had higher richness and distinct community compositions,relative to winter *** both summer and winter samples,the diversity of recycled sand decreased with time drying in the recycling ***,summer sand 14 d post-recycling was enriched in operational taxonomic units(OTUs)belonging to the genera Acinetobacter and Pseudomonas,relative to freshly washed sand and sand from cow *** contrast,no OTUs were found to be enriched in winter *** sand recycling system contained an overall core microbiota of 141 OTUs representing 68.45%±10.33%SD of the total bacterial relative abundance at each sampled *** 4 most abundant genera in this core microbiota included Acinetobacter,Psychrobacter,Corynebacterium,and *** was present in greater abundance in summer samples,whereas Psychrobacter and Corynebacterium had higher relative abundances in winter *** had consistent relative abundances across both ***:These findings highlight the potential of recycled bedding sand as a bacterial reservo

关键词： Bovine Dairy farm Microbiota Recycled bedding sand 16S rRNA sequencing

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Assessing the relationship between the rumen microbiota and feed efficiency in Nellore steers

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Journal of Animal Science and Biotechnology 2021年第4期12卷 1645-1661页

作者： Déborah Romaskevis Gomes Lopes Márcio de Souza Duarte Alex J.La Reau Ibrahim Zuniga Chaves Tiago Antônio de Oliveira Mendes Edenio Detmann Cláudia Braga Pereira Bento Maria Eugênia Zerlotti Mercadante Sarah Figueiredo Martins Bonilha garret suen Hilário Cuquetto Mantovani Departamento de Microbiologia Universidade Federal de ViçosaViçosaMG 36570-900Brazil Departamento de Zootecnia Universidade Federal de ViçosaViçosaMinas GeraisBrazil Department of Bacteriology University of Wisconsin-MadisonMadisonWIUSA Departamento de Bioquímia e Biologia Molecular Universidade Federal de ViçosaViçosaMinas GeraisBrazil Instituto de Zootecnia Centro APTA Bovinos de CorteSertãozinhoSão PauloBrazil

Background:Ruminants rely upon a complex community of microbes in their rumen to convert host-indigestible feed into ***,little is known about the association between the rumen microbiota and feed efficiency traits in Nellore(Bos indicus)cattle,a breed of major economic importance to the global beef ***,we compare the composition of the bacterial,archaeal and fungal communities in the rumen of Nellore steers with high and low feed efficiency(FE)phenotypes,as measured by residual feed intake(RFI).Results:The Firmicutes to Bacteroidetes ratio was significantly higher(P<0.05)in positive-RFI steers(p-RFI,low feed efficiency)than in negative-RFI(n-RFI,high feed efficiency)*** differences in bacterial composition from steers with high and low FE were mainly associated with members of the families Lachnospiraceae,Ruminococcaceae and Christensenellaceae,as well as the genus *** community richness was lower(P<0.05)in p-RFI than in n-RFI steers and the genus Methanobrevibacter was either increased or exclusive of p-RFI *** fungal genus Buwchfawromyces was more abundant in the rumen solid fraction of n-RFI steers(P<0.05)and a highly abundant OTU belonging to the genus Piromyces was also increased in the rumen microbiota of highefficiency ***,analysis of rumen fermentation variables and functional predictions indicated similar metabolic outputs for the microbiota of distinct FE ***:Our results demonstrate that differences in the ruminal microbiota of high and low FE Nellore steers comprise specific taxa from the bacterial,archaeal and fungal *** OTUs belonging to the genus Piromyces were identified in animals showing high feed efficiency,whereas among archaea,Methanobrevibacter was associated with steers classified as *** identification of specific RFI-associated microorganisms in Nellore steers could guide further studies targeting the isolation and functional characterization

关键词： Beef cattle ITS1 region Next-generation sequencing RFI Rumen microbiota 16S rRNA gene

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The ruminal bacterial community in lactating dairy cows has limited variation on a day-to-day basis.

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畜牧与生物技术杂志(英文版) 2019年第4期

作者： Joseph H.Skarlupka Maria E.Kamenetsky Kelsea A.Jewell garret suen

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Assessing the microbiota of recycled bedding sand on a Wisconsin dairy farm.

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畜牧与生物技术杂志(英文版) 2022年第2期

作者： Hannah E.Pilch Andrew J.Steinberger Donald C.Sockett Nicole Aulik garret suen Charles J.Czuprynski

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Assessing the relationship between the rumen microbiota and feed efficiency in Nellore steers.

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畜牧与生物技术杂志(英文版) 2021年第4期

作者： Déborah Romaskevis Gomes Lopes Márcio de Souza Duarte Alex J.La Reau Ibrahim Zuniga Chaves Tiago Ant?nio de Oliveira Mendes Edenio Detmann Cláudia Braga Pereira Bento Maria Eugênia Zerlotti Mercadante Sarah Figueiredo Martins Bonilha garret suen Hilário Cuquetto Mantovani

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