检索结果-南通市图书馆

红麻酮脂酰辅酶A合成酶(KCS)酵母双杂交诱饵载体构建及自激活检测

在线全文

学校读者我要写书评

暂无评论

中国麻业科学 2018年第2期40卷 75-78页

作者：张高阳邓接楼黄思齐李德芳江西上饶师范学院生命科学学院江西上饶334001 中国农业科学院麻类研究所长沙410205

酮脂酰辅酶A合成酶(KCS)是超长链脂肪酸合成过程中的限速酶,参与超长链脂肪酸和蜡质的合成,在植物抗旱和应对生物胁迫反应中发挥重要调控作用。研究采用PCR方法从红麻中(茎皮)分离KCS基因片段,将该基因按正确方式插入酵母双杂交表达质粒... 详细信息

酮脂酰辅酶A合成酶(KCS)是超长链脂肪酸合成过程中的限速酶,参与超长链脂肪酸和蜡质的合成,在植物抗旱和应对生物胁迫反应中发挥重要调控作用。研究采用PCR方法从红麻中(茎皮)分离KCS基因片段,将该基因按正确方式插入酵母双杂交表达质粒pGBKT7中,酶切鉴定正确后,用PEG/Li Ac方法转化酵母MaV203,经抗性筛选后,用X-Gal对转化酵母进行自激活检测。结果表明,KCS基因的DNA结合结构域与酵母GAL4转录因子的上游激活位点(UAS)结合,可激活报告基因半乳糖苷酶的表达。经删除KCS基因DNA binding区域后重新转化酵母,结果表明,不携带KCS基因DNA binding区域的截短蛋白可以作为诱饵蛋白来研究与之相互作用的蛋白。

关键词：酮脂酰辅酶A合成酶酵母双杂交自激活红麻

在线全文

学校读者我要写书评

暂无评论

冬凌草药渣固体发酵工艺条件优化

河南农业科学 2024年第3期53卷 169-180页

作者：陈璐周省委徐可可张高阳刘汶泽曹小凤杨林林董诚明河南中医药大学药学院河南郑州450046 河南省道地药材生态种植工程技术研究中心河南郑州450046

为筛选出冬凌草药渣固体发酵工艺的最适条件,研究不同接种量、不同料液比、不同发酵时间、不同发酵温度处理下酵母菌、乳酸菌固体发酵工艺对冬凌草药渣中齐墩果酸、熊果酸、粗蛋白、粗纤维、粗脂肪、钙、粗灰分、赖氨酸含量变化的影响,... 详细信息

为筛选出冬凌草药渣固体发酵工艺的最适条件,研究不同接种量、不同料液比、不同发酵时间、不同发酵温度处理下酵母菌、乳酸菌固体发酵工艺对冬凌草药渣中齐墩果酸、熊果酸、粗蛋白、粗纤维、粗脂肪、钙、粗灰分、赖氨酸含量变化的影响,并对其进行相关性和主成分分析。结果表明,酵母菌固体发酵最优条件:接种量6%、料液比1∶1.2、发酵时间3 d、发酵温度28℃。此条件下,粗蛋白、粗脂肪、钙、粗灰分、齐墩果酸、熊果酸含量分别相较于发酵前提高0.93%、23.08%、13.89%、19.74%、15.00%、16.22%,粗纤维含量相较于发酵前减少1.19%。齐墩果酸含量与熊果酸含量相关性达到0.86,呈极显著正相关,粗蛋白含量与钙、粗灰分、赖氨酸含量相关性分别达到0.95、0.97、0.90,呈极显著正相关。乳酸菌固体发酵最优条件:接种量6%、料液比1∶1.2、发酵时间6 d、发酵温度28℃。此条件下,粗蛋白、粗脂肪、钙、粗灰分、赖氨酸含量分别相较于发酵前提高33.02%、23.08%、74.07%、51.32%、21.21%,粗纤维含量相较于发酵前减少24.76%。粗蛋白含量与赖氨酸含量相关性达到0.77,呈极显著正相关。综上,酵母菌和乳酸菌固体发酵方法均可使冬凌草药渣的营养价值得到提高。

关键词：冬凌草药渣固体发酵发酵工艺酵母菌乳酸菌

改良CTAB法提取不同作物总RNA技术研究

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

中国农学通报 2024年第11期40卷 28-35页

作者：刘国梅郭淑慧孙璇姚琳张高扬杜春芳山西农业大学农学院山西晋中030801 山西农业大学棉花研究所山西运城044000

为提高操作效率,本研究开发出一种简洁有效的作物总RNA提取方法。研究以油菜、棉花、花生、小麦、马铃薯、玉米、大豆、西葫芦等作物的不同组织为试材,通过CTAB法、改良CTAB法及TanSzol Reagent试剂盒法提取总RNA,检测结果发现,经过改... 详细信息

为提高操作效率,本研究开发出一种简洁有效的作物总RNA提取方法。研究以油菜、棉花、花生、小麦、马铃薯、玉米、大豆、西葫芦等作物的不同组织为试材,通过CTAB法、改良CTAB法及TanSzol Reagent试剂盒法提取总RNA,检测结果发现,经过改良后的CTAB法提取的总RNA质量较高。在油菜花瓣、小麦、花生、西葫芦叶片中,该方法获得了较高的总RNA得率,分别为24.5、23.7、26.4、17.35μg/mL;OD260/280和OD260/230值分别为1.90、1.73、1.72、1.80和2.10、1.50、2.01、1.70。综合比较以上3种方法,改良后的CTAB法适用于油菜、小麦、花生、西葫芦等作物的总RNA提取,为后续分子生物学检测奠定了基础。

关键词：改良CTAB法总RNA RNA提取

晋南地区不同甘蓝型油菜品系菜薹的生物学性状及产质量

在线全文

学校读者我要写书评

暂无评论

贵州农业科学 2022年第3期50卷 18-23页

作者：赵卫喆孙璇姚琳咸拴狮张高杨杜春芳山西农业大学棉花研究所山西运城044000

【目的】探明不同甘蓝型油菜品系菜薹在晋南地区的生物学性状、营养品质及产量差异,为选育和推广适合晋南地区种植的优质高产油菜新品系菜薹提供理论依据。【方法】以20个不同甘蓝型油菜双低品系为研究对象,主要测定菜薹的生物学性状、... 详细信息

【目的】探明不同甘蓝型油菜品系菜薹在晋南地区的生物学性状、营养品质及产量差异,为选育和推广适合晋南地区种植的优质高产油菜新品系菜薹提供理论依据。【方法】以20个不同甘蓝型油菜双低品系为研究对象,主要测定菜薹的生物学性状、营养品质及产量等11个指标,并结合Delphi法对各油菜品系菜薹进行综合排序。【结果】参试的20个不同甘蓝型油菜双低品系菜薹的生物学性状、营养品质及产量均存在较大差异。菜薹产量以yc2002最高,达4166.7 kg/hm^(2),较CK增加33.0%;Vc以yc2001最高,为93.31 mg/100g,较CK高2.55%;类胡萝卜素以yc2015最高,达335.99 ug/g-1fw,较CK高36.13%;叶绿素以yc2007最高,为54.62,较CK高14.58;可溶性糖以yc2018最高,为5.30%,较CK高79.05%。【结论】yc2001、yc2002、yc2003、yc2004、yc2010和yc2015的综合表现均优于CK(秦油7号),适宜作为晋南地区“油蔬两用”油菜品系推广,其中,yc2001品系菜薹综合品质优良,表现为茎粗、薹叶多、叶色绿和产量高等特点,可作为首推品系。

关键词：甘蓝型油菜菜薹生物学性状品质

在线全文

学校读者我要写书评

暂无评论

智能奶瓶清洗机的设计

科技创新导报 2021年第10期18卷 47-50页

作者：张高扬杨丙辉张文杰罗水琼林欣悦刘海龙林志康塔里木大学机械电气化工程学院新疆阿拉尔843300

新型高效的奶瓶清洗工具将会成为婴儿母亲的必需品之一,为此设计一种智能奶瓶清洗机将尤为必要。本文将对智能奶瓶清洗机的设计进行论述,主要包括结构设计的理论和相关设计的应用。智能奶瓶清洗机的设计主要采用计算机辅助设计进行,先... 详细信息

新型高效的奶瓶清洗工具将会成为婴儿母亲的必需品之一,为此设计一种智能奶瓶清洗机将尤为必要。本文将对智能奶瓶清洗机的设计进行论述,主要包括结构设计的理论和相关设计的应用。智能奶瓶清洗机的设计主要采用计算机辅助设计进行,先通过计算机建模设计出智能奶瓶清洗机的模型,再将模型各组份拆分,然后分别对模型进行可行性分析和参数调整实验,得出模型修正和改进数据,最终根据此数据指导智能奶瓶清洗机的设计。

关键词：智能清洗奶瓶清洗机紫外线消毒 51单片机

Therapy for benign rectal anastomotic stricture after surgery for colorectal cancer:A systematic review

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Laparoscopic, Endoscopic and Robotic Surgery 2024年第4期7卷 166-173页

作者： gaoyang Cao Songtao Wu Li zhang Xinjie zhang Wei Zhou Department of Colorectal Surgery Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Department of Clinical Medicine Zhejiang University School of MedicineHangzhou 310030China Department of General Surgery Tan Tock Seng HospitalSingapore 308433Singapore

Objective:To evaluate various treatment methods for benign rectal anastomotic stricture(AS)following surgery for colorectal ***:A systematic review of the literature was conducted,focusing on studies that reported outcomes of different treatment modalities for benign *** PubMed,Embase,Scopus,China National Knowledge Infrastructure,and Cochrane Library databases were searched from January 2000 to December *** inclusion criteria were studies involving human subjects,published in English,and reporting on therapeutic outcomes for benign ***:A total of 19 papers identified a range of therapeutic strategies,including nonoperative anastomotic dilation,endoscopic balloon dilation(EBD),transanal minimally invasive surgery(TAMIS),selfexpandable metal stents(SEMS),endoscopic incision(EI)and newer techniques such as prostate resection *** anastomotic dilation can serve as an initial treatment for lower *** demonstrated promise in cases where EBD was ineffective,providing an alternative method for managing *** and SEMS showed higher efficacy in refractory cases,with TAMIS being particularly effective for severe fibrotic or completely closed *** use of rigid instrumentation with an electric knife for transanal incisions demonstrated precision but lacked the flexibility needed for complex ***:While traditional methods such as nonoperative anastomotic dilation and EBD remain firstline treatments for benign AS,advanced techniques such as EI,TAMIS,and SEMS offer promising alternatives,particularly in refractory *** choice of treatment should be tailored to individual patient conditions,with consideration for the technical expertise required and the potential for complications.

关键词： Rectal anastomotic stricture Colorectal cancer Therapy

Damming effects on dissolved inorganic carbon in different kinds of reservoirs in Jialing River,Southwest China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Geochimica 2017年第4期36卷 581-597页

作者： gaoyang Cui Xiaodong Li Qinkai Li Jun Huang Yuele Tao Siqi Li Jun zhang State Key Laboratory of Environmental Geochemistry Institute of GeochemistryChinese Academy of SciencesGuiyang 550081People's Republic of China University of Chinese Academy of Sciences Beijing 100049People's Republic of China Institute of Surface-Earth System Science Tianjin UniversityTianjin 300072People's Republic of China

To assess the effects of river damming on dissolved inorganic carbon in the Jialing River, a total of 40 water samples, including inflow, outflow, and stratified water in four cascade reservoirs(Tingzikou, Xinzheng,Dongxiguan, Caojie) were collected in January and July,2016. The major cations, anions, and δ^(13)C_(DIC) values were analyzed. It was found that the dissolved compositions are dominated by carbonate weathering, while sulfuric acids may play a relatively important role during carbonate weathering and increasing DIC concentration. Different reservoirs had variable characteristics of water physiochemical stratification. The DIC concentrations of reservoir water were lower in summer than those in winter due to the dilute effects and intensive aquatic photosynthesis, as well as imported tributaries. The δ^(13)C_(DIC) values in Tingzikou Reservoir were higher during summer than those in winter,which indicated that intensive photosynthesis increased the δ^(13)C_(DIC) values in residual water, but a similar trend was not obvious in other reservoirs. Except for in Xinzheng Reservoir, the δ^(13)C_(DIC) values in inflow and outflow reservoir water were lower than those in the surface water of stratified sampling in summer. For stratified sampling, it could be found that, in summer, the Tingzikou Reservoir δ^(13)C_(DIC) values significantly decreased with water depthdue to the anaerobic breakdown of organic matter. The significant correlation(p<0.01 or 0.05) between the DIC concentrations, the δ^(13)C_(DIC) values and anthropogenic species(Na^++K^+, Cl~–, SO_4^(2-) and NO_3^-) showed that the isotope composition of DIC can be a useful tracer of contaminants. In total, Tingzikou Reservoir showed lacustrine features, Xinzheng Reservoir and Dongxiguan Reservoir had "transitional'' features, and Caojie Reservoir had a total of "fluvial'' features. Generally, cascade reservoirs in the Jialing River exhibited natural river features rather than typical lake features due to

关键词： River damming Water chemistry Reservoir types Dissolved inorganic carbon isotope composition DIC concentration

The mutation of ent-kaurene synthase,a key enzyme involved in gibberellin biosynthesis,confers a non-heading phenotype to Chinese cabbage(Brassica rapa ***)

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2020年第1期7卷 321-330页

作者： Yue Gao Shengnan Huang gaoyang Qu Wei Fu Meidi zhang Zhiyong Liu Hui Feng Liaoning Key Laboratory of Genetics and Breeding for Cruciferous Vegetable Crops College of HorticultureShenyang Agricultural University110866 ShenyangChina

The presence of a leafy head is a vital agronomic trait that facilitates the evaluation of the yield and quality of Chinese cabbage.A non-heading mutant(nhm1)was identified in an ethyl methanesulfonate mutagenesis population of the heading Chinese cabbage double haploid line *** analysis revealed that a single recessive gene,Brnhm1,controlled the mutant *** MutMap,Kompetitive allele-specific PCR,and cloning analyses,we demonstrated that BraA07g042410.3C,which encodes an ent-kaurene synthase involved in the gibberellin biosynthesis pathway,is the nhm1 mutant candidate gene.A single-nucleotide mutation(C to T)in the fourth exon of BraA07g042410.3C caused an amino acid substitution from histidine to *** to that of the wild-type FT,BraA07g042410.3C in the leaves of the nhm1 mutant had lower levels of *** addition,gibberellin contents were lower in the mutant than in the wild type,and the mutant plant phenotype could be restored to that of the wild type after exogenous GA3 *** results indicate that BraA07g042410.3C caused the non-heading *** is the first study to demonstrate a relationship between gibberellin content in the leaves and leafy head formation in Chinese *** findings facilitate the understanding of the mechanisms underlying leafy head development in Chinese cabbage.

关键词： substitution methane heading

The LA-ICP-MS zircons U-Pb ages and geochemistry of the Baihua basic igneous complexes in Tianshui area of West Qinling

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Earth Sciences 2007年第Z2期50卷 264-276页

作者： PEI XianZhi DING SaPing zhang GuoWei LIU HuiBin LI ZuoChen LI gaoyang LIU ZhanQing MENG Yong Key Laboratory of Western China's Mineral Resources and Geological Engineering Ministry of Education Faculty of Earth Science and Land Resources Chang'an University Xi'an 710054 China Key Laboratory of Western China's Mineral Resources and Geological Engineering Ministry of Education Faculty of Earth Science and Land Resources Chang'an University Xi'an 710054 China State Key Laboratory of Continental Dynamics Department of Geology Northwest University Xi'an 710069 China Key Laboratory of Western China's Mineral Resources and Geological Engineering Ministry of Education Faculty of Earth Science and Land Resources Chang'an University Xi'an 710054 China

Baihua meta-igneous complex consists mainly of pyroxenite-gabbro(diorite)-diorite-quartz diorite. They form a complete comagmatic evolutionary series. The geochemical characteristics of basic-interme- diate basic igneous rocks indicate that they belong to a tholeiite suite. The REE distribution pattern is nearly flat type and LREE is slightly enriched type, and their primitive mantle-normalized and MORB-normalized trace element spider diagrams are generally similar; the LIL elements (LILE) Cs, Ba, Sr, Th and U are enriched, but Rb, K and the HFSEs Nb, P, Zr, Sm, Ti and Y are relatively depleted. All these show comagmatic evolution and origin characteristics. The tectonics environment discrimination of trace element reveals that these igneous complexes formed in an island-arc setting. The LA-ICP-MS single-zircons U-Pb age of Baihua basic igneous complex is 434.6±1.5 Ma (MSWD = 1.3), which proves that the formation time of the island-arc type magmatite in the northern zone of West Qinling is Late Ordovician or Early Silurian, also reveals that the timing of subduction of paleo-ocean basin represented by the Guanzizhen ophiolite and resulting island-arc-type magmatic activities is probably Middle-Late Ordovician to Early Silurian.

关键词： basic igneous complex, island-arc setting, LA-ICP-MS, zircons U-Pb isotopic ages, Tianshui area, West Qinling