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Long noncoding RNAs HAND2-AS1 ultrasound microbubbles suppress hepatocellular carcinoma progression by regulating the miR-873-5p/tissue inhibitor of matrix metalloproteinase-2 axis

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World Journal of Gastrointestinal Oncology 2024年第4期16卷 1547-1563页

作者： Qiang Zou Hao-Wen Wang xi-liang Di Yuan li Hui gao Department of Interventional Therapy Tianjin Medical University Cancer Institute and HospitalTianjin 300060China National Clinical Research Center for Cancer Key Laboratory of Cancer Prevention and TherapyTianjin’s Clinical Research Center for CancerTianjin 300060China College of Animal Science and Technology Northeast Agricultural UniversityHarbin 150030Heilongjiang ProvinceChina Department of Hematology and Oncology Linyi People’s HospitalLinyi 251500Shandong ProvinceChina Department of Comprehensive Oncology Baotou Cancer HospitalBaotou 014030Inner Mongolia Autonomous RegionChina

BACKGROUND Increasing data indicated that long noncoding RNAs(lncRNAs)were directly or indirectly involved in the occurrence and development of tumors,including hepatocellular carcinoma(HCC).Recent studies had found that the expression of lncRNA HAND2-AS1 was downregulated in HCC tissues,but its role in HCC progression is unclear.Ultrasound targeted microbubble destruction mediated gene transfection is a new method to overexpress genes.AIM To study the role of ultrasound microbubbles(UTMBs)mediated HAND2-AS1 in the progression of HCC,in order to provide a new reference for the treatment of HCC.METHODS In vitro,we transfected HAND2-AS1 siRNA into HepG2 cells by UTMBs,and detected cell proliferation,apoptosis,invasion and epithelial-mesenchymal transition(EMT)by cell counting kit-8 assay,flow cytometry,Transwell invasion assay and Western blotting,respectively.In addition,we transfected miR-837-5p mimic into UTMBs treated cells and observed the changes of cell behavior.Next,the UTMBs treated HepG2 cells were transfected together with miR-837-5p mimic and tissue inhibitor of matrix metalloproteinase-2(TIMP2)overexpression vector,and we detected cell proliferation,apoptosis,invasion and EMT.In vivo,we established a mouse model of subcutaneous transplantation of HepG2 cells and observed the effect of HAND2-AS1 silencing on tumor formation ability.RESULTS We found that UTMBs carrying HAND2-AS1 restricted cell proliferation,invasion,and EMT,encouraged apoptosis,and HAND2-AS1 silencing eliminated the effect of UTMBs.Additionally,miR-873-5p targets the gene HAND2-AS1,which also targets the 3’UTR of TIMP2.And miR-873-5p mimic counteracted the impact of HAND2-AS1.Further,miR-873-5p mimic solely or in combination with pcDNA-TIMP2 had been transformed into HepG2 cells exposed to UTMBs.We discovered that TIMP2 reversed the effect of miR-873-5p mimic caused by the blocked signalling cascade for matrix metalloproteinase(MMP)2/MMP9.In vivo results showed that HAND2-AS1 silencing significantly inhibited tumor formation in mice.CON

关键词： Hepatocellular carcinoma Ultrasound microbubbles Long noncoding RNA HAND2-AS1 miR-873-5p Tissue inhibitor of matrix metalloproteinase-2

Application of GNSS-PPP on Dynamic Deformation Monitoring of Offshore Platforms

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China Ocean Engineering 2024年第2期38卷 352-361页

作者： YU li-na xiONG Kuan gao xi-feng li Zhi FAN li-long ZHANG Kai Institute of Ocean Energy and Intelligent Construction Tianjin University of TechnologyTianjin 300384China China Railway Construction Bridge Engineering Bureau Group Co.LTD Tianjin 300300China School of Civil Engineering Tianjin UniversityTianjin 300072China Department of Land Surveying and Geo-Informatics The Hong Kong Polytechnic UniversityHong KongChina State Key Laboratory of Precision Blasting Jianghan UniversityWuhan 430056China

The real-time dynamic deformation monitoring of offshore platforms under environmental excitation is crucial to their safe operation.Although Global Navigation Satellite System-Precise Point Positioning(GNSS-PPP)has been considered for this purpose,its monitoring accuracy is relatively low.Moreover,the influence of background noise on the dynamic monitoring accuracy of GNSS-PPP remains unclear.Hence,it is imperative to further validate the feasibility of GNSS-PPP for deformation monitoring of offshore platforms.To address these concerns,vibration table tests with different amplitudes and frequencies are conducted.The results demonstrate that GNSS-PPP can effectively monitor horizontal vibration displacement as low as±30 mm,which is consistent with GNSS-RTK.Furthermore,the spectral characteristic of background noise in GNSS-PPP is similar to that of GNSS-RTK(Real Time Kinematic).Building on this observation,an improved Complete Ensemble Empirical Mode Decomposition with Adaptive Noise(CEEMDAN)has been proposed to de-noise the data and enhance the dynamic monitoring accuracy of GNSS-PPP.Field monitoring application research is also undertaken,successfully extracting and analyzing the dynamic deformation of an offshore platform structure under environmental excitation using GNSS-PPP monitoring in conjunction with improved CEEMDAN de-noising.By comparing the de-noised dynamic deformation trajectories of the offshore platform during different periods,it is observed that the platform exhibits reversible alternating vibration responses under environmental excitation,with more pronounced displacement deformation in the direction of load action.The research results confirm the feasibility and potential of GNSS-PPP for dynamic deformation monitoring of offshore platforms.

关键词： GNSS-PPP offshore platform dynamic deformation monitoring improved CEEMDAN de-noising

An improved transfer learning strategy for short-term cross-building energy prediction usingdata incremental

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Building Simulation 2024年第1期17卷 165-183页

作者： Guannan li Yubei Wu Chengchu Yan xi Fang Tao li Jiajia gao Chengliang Xu Zixi Wang School of Urban Construction Wuhan University of Science and TechnologyWuhan 430065China Anhui Province Key Laboratory of Intelligent Building and Building Energy-saving Anhui Jianzhu UniversityHefei 230601China State Key Laboratory of Green Building in Western China Xi'an University of Architecture and TechnologyXi'an 710055China College of Urban Construction Nanjing Tech UniversityNo.200North Zhongshan RoadNanjing 210009China Key Laboratory of Low-grade Energy Utilization Technologies and Systems(Chongqing University) Ministry of Education of ChinaChongqing UniversityChongqing 400044China College of Civil Engineering Hunan UniversityChangsha 410082China Hubei Provincial Engineering Research Center of Urban Regeneration Wuhan University of Science and TechnologyWuhan 430065China

The available modelling data shortage issue makes it difficult to guarantee the performance of data-driven building energy prediction(BEP)models for both the newly built buildings and existing information-poor buildings.Both knowledge transfer learning(KTL)and data incremental learning(DIL)can address the data shortage issue of such buildings.For new building scenarios with continuous data accumulation,the performance of BEP models has not been fully investigated considering the data accumulation dynamics.DIL,which can learn dynamic features from accumulated data adapting to the developing trend of new building time-series data and extend BEP model's knowledge,has been rarely studied.Previous studies have shown that the performance of KTL models trained with fixed data can be further improved in scenarios with dynamically changing data.Hence,this study proposes an improved transfer learning cross-BEP strategy continuously updated using the coarse data incremental(CDI)manner.The hybrid KTL-DIL strategy(LSTM-DANN-CDI)uses domain adversarial neural network(DANN)for KLT and long short-term memory(LSTM)as the Baseline BEP model.Performance evaluation is conducted to systematically qualify the effectiveness and applicability of KTL and improved KTL-DIL.Real-world data from six-type 36 buildings of six types are adopted to evaluate the performance of KTL and KTL-DIL in data-driven BEP tasks considering factors like the model increment time interval,the available target and source building data volumes.Compared with LSTM,results indicate that KTL(LSTM-DANN)and the proposed KTL-DIL(LSTM-DANN-CDI)can significantly improve the BEP performance for new buildings with limited data.Compared with the pure KTL strategy LSTM-DANN,the improved KTL-DIL strategy LSTM-DANN-CDI has better prediction performance with an average performance improvement ratio of 60%.

关键词： building energy prediction(BEP) cross-building data incremental learning(DIL) domain adversarial neural network(DANN) knowledge transfer learning(KTL)

Strain-promoted S-arylation and alkenylation of sulfinamides using arynes and cyclic alkynes

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Science China Chemistry 2024年第3期67卷 928-935页

作者： xi Zou Boming Shen gao-lin li Qian liang Yanhua Ouyang Binghe Yang Peiyuan Yu Bing gao State Key Laboratory of Chemo/Bio-Sensing and Chemometrics College of Chemistry and Chemical EngineeringInstitute of Chemical Biology and NanomedicineHunan UniversityChangsha 410082China Department of Chemistry and Shenzhen Grubbs Institute Guangdong Provincial Key Laboratory of CatalysisSouthern University of Science and TechnologyShenzhen 518055China

The conversion of commercially available chiral sulfinamides into pharmaceutically useful chiral sulfoximines via direct SIVfunctionalization is synthetically attractive but challenging due to the competitive reaction of N-functionalization. Herein, we disclose a novel strain-release strategy to access stereospecific and chemoselective SIV-arylation and alkenylation of sulfinamides using arynes and strained cyclic alkynes. This method tolerates an unprecedented chemical diversity of functional groups attached to the nitrogen center(N-R). The origin of the high SIV-selectivity is elucidated by density functional theory calculations, suggesting a stepwise mechanism for the aryne substrates and a concerted mechanism for the cyclic alkynes.

关键词： arylation alkenylation sulfoximines sulfinamides arynes

HepG2.2.15-derived exosomes facilitate the activation and fibrosis of hepatic stellate cells

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World Journal of Gastroenterology 2024年第19期30卷 2553-2563页

作者： Yang gao li li Sheng-Ning Zhang Yuan-Yi Mang xi-Bing Zhang Shi-Ming Feng Department of Hepatobiliary Pancreatic and Vascular Surgery The Affiliated Calmette Hospital of Kunming Medical University and The First Hospital of KunmingKunming 650011Yunnan ProvinceChina

BACKGROUND The role of exosomes derived from HepG2.2.15 cells,which express hepatitis B virus(HBV)-related proteins,in triggering the activation of LX2 liver stellate cells and promoting liver fibrosis and cell proliferation remains elusive.The focus was on comprehending the relationship and influence of differentially expressed microRNAs(DE-miRNAs)within these exosomes.AIM To elucidate the effect of exosomes derived from HepG2.2.15 cells on the activation of hepatic stellate cell(HSC)LX2 and the progression of liver fibrosis.METHODS Exosomes from HepG2.2.15 cells,which express HBV-related proteins,were isolated from parental HepG2 and WRL68 cells.Western blotting was used to confirm the presence of the exosomal marker protein CD9.The activation of HSCs was assessed using oil red staining,whereas DiI staining facilitated the observation of exosomal uptake by LX2 cells.Additionally,we evaluated LX2 cell proliferation and fibrosis marker expression using 5-ethynyl-2′-deoxyuracil staining and western blotting,respectively.DE-miRNAs were analyzed using DESeq2.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways were used to annotate the target genes of DE-miRNAs.RESULTS Exosomes from HepG2.2.15 cells were found to induced activation and enhanced proliferation and fibrosis in LX2 cells.A total of 27 miRNAs were differentially expressed in exosomes from HepG2.2.15 cells.GO analysis indicated that these DE-miRNA target genes were associated with cell differentiation,intracellular signal transduction,negative regulation of apoptosis,extracellular exosomes,and RNA binding.KEGG pathway analysis highlighted ubiquitin-mediated proteolysis,the MAPK signaling pathway,viral carcinogenesis,and the toll-like receptor signaling pathway,among others,as enriched in these targets.CONCLUSION These findings suggest that exosomes from HepG2.2.15 cells play a substantial role in the activation,proliferation,and fibrosis of LX2 cells and that DE-miRNAs within these exosomes contribute to the underlying mechanisms.

关键词： Hepatic stellate cells liver fibrosis Exosomes Small RNA sequencing HepG2.2.15

Endoscopic treatment of bleeding gastric ulcer causing gastric wall necrosis:A case report

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World Journal of Gastrointestinal Surgery 2024年第4期16卷 1203-1207页

作者： Wei-Feng li Ruo-Yu gao Jing-Wen Xu xi-Qiu Yu Department of Gastroenterology Shenzhen Luohu People’s HospitalShenzhen 518020Guangdong ProvinceChina

BACKGROUND Gastric wall necrosis is a rare complication of endoscopic treatment for bleeding gastric ulcer,which may exacerbate the patient’s condition once it occurs and may even require surgical intervention for treatment.CASE SUMMARY A 59-year-old man was admitted to our department with melena.Endoscopy revealed a giant ulcer in the gastric antrum with a visible vessel in its center,which was treated with sclerosants and tissue glue injection and resulted in necrosis of the gastric wall.CONCLUSION Injection of sclerosants and tissue glue may lead to gastric wall necrosis,which is a serious complication.Therefore,before administering this treatment to patients,we should consider other more effective methods of hemostasis to avoid gastric wall necrosis.

关键词： Bleeding gastric ulcer Sclerotherapy Lauromacrogol Tissue adhesive Gastric wall necrosis Case report

Comprehensive evaluation of rare case:From diagnosis to treatment of a sigmoid Schwannoma:A case report

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World Journal of Gastrointestinal Oncology 2024年第1期16卷 244-250页

作者： Jing-Yi li xi-Zhuang gao Jian Zhang xiang-Zheng Meng Yi-xian Cao Kun Zhao Clinical Medical College of Jining Medical University Jining Medical UniversityJining 272000Shandong ProvinceChina Department of Gastroenterology The First People's Hospital of JiningJining 272000Shandong ProvinceChina

BACKGROUND Schwannomas are uncommon tumors originating from Schwann cells,forming the neural sheath.They account for approximately 2%-6%of all mesenchymal tumors and are most commonly identified in peripheral nerve trunks,with rarity in the gastrointestinal tract.Among gastrointestinal locations,the stomach harbors the majority of nerve sheath tumors,while such occurrences in the sigmoid colon are exceptionally infrequent.CASE SUMMARY This study presented a clinical case involving a 60-year-old female patient who,during colonoscopy,was diagnosed with a submucosal lesion that was later identified as a nerve sheath tumor.The patient underwent surgical resection,and the diagnosis was confirmed through immunohistochemistry.This study highlighted an exceptionally uncommon occurrence of a nerve sheath tumor in the sigmoid colon,which was effectively managed within our department.Additionally,a comprehensive review of relevant studies was conducted.CONCLUSION The preoperative diagnosis of nerve sheath tumors poses challenges,as the definitive diagnosis still relies on pathology and immunohistochemistry.Although categorized as benign,these tumors have the potential to demonstrate malignant behavior.Consequently,the optimal treatment approach entails the complete surgical excision of the tumor,ensuring the absence of residual lesions at the margins.

关键词： Schwannoma Gastrointestinal stromal tumors Treatment Sigmoid colon Case report

Activation of G-protein-coupled receptor 39 reduces neuropathic pain in a rat model

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Neural Regeneration Research 2024年第3期19卷 687-696页

作者： Longqing Zhang xi Tan Fanhe Song Danyang li Jiayi Wu Shaojie gao Jia Sun Daiqiang liu Yaqun Zhou Wei Mei Department of Anesthesiology Hubei Key Laboratory of Geriatric Anesthesia and Perioperative Brain Healthand Wuhan Clinical Research Center for Geriatric AnesthesiaTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Activated G-protein-coupled receptor 39(GPR39)has been shown to attenuate inflammation by interacting with sirtuin 1(SIRT1)and peroxisome proliferator-activated receptor-γcoactivator 1α(PGC-1α).However,whether GPR39 attenuates neuropathic pain remains unclear.In this study,we established a Sprague-Dawley rat model of spared nerve injury-induced neuropathic pain and found that GPR39 expression was significantly decreased in neurons and microglia in the spinal dorsal horn compared with sham-operated rats.Intrathecal injection of TC-G 1008,a specific agonist of GPR39,significantly alleviated mechanical allodynia in the rats with spared nerve injury,improved spinal cord mitochondrial biogenesis,and alleviated neuroinflammation.These changes were abolished by GPR39 small interfering RNA(siRNA),Ex-527(SIRT1 inhibitor),and PGC-1αsiRNA.Taken together,these findings show that GPR39 activation ameliorates mechanical allodynia by activating the SIRT1/PGC-1αpathway in rats with spared nerve injury.

关键词： G-protein-coupled receptor 39(GPR39) neuroinflammation neuropathic pain nuclear respiratory factor 1(NRF1) peroxisome proliferator-activated receptor-γcoactivator 1α(PGC-1α) sirtuin 1(SIRT1) spinal cord mitochondrial transcription factor A(TFAM)

Evolution of HIV/AIDS Prevention and Control Policies in China:A Grounded Theory Approach

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China CDC weekly 2024年第1期6卷 12-22页

作者： Huang li Rui gao Chichen Zhang School of Public Health Southern Medical UniversityGuangzhou CityGuangdong ProvinceChina School of Health Management Southern Medical UniversityGuangzhou CityGuangdong ProvinceChina Key Laboratory of Philosophy and Social Sciences of Colleges and Universities in Guangdong Province for Collaborative Innovation of Health Management Policy and Precision Health Service Southern Medical UniversityGuangzhou CityGuangdong ProvinceChina Graduate School Xi’an Jiaotong-Liverpool UniversitySuzhou CityJiangsu ProvinceChina School of Political Science and Public Administration Soochow UniversitySuzhou CityJiangsu ProvinceChina

Objective:This study aims to provide a theoretical foundation for the development and practical application of human immunodeficiency virus(HIV)/acquired immunodeficiency syndrome(AIDS)prevention and control policy systems in China by scrutinizing,analyzing,and synthesizing the evolution of Chinese policies in this domain.Methods:Utilizing grounded theory,we employed NVivo12 software to perform text content analysis,mining,and coding classification,along with visualization techniques,on policy texts sourced from Chinese government platforms,including the official website of China’s State Council.We considered four analytical dimensions—time,subject,type,and object.Results:The National Health Commission and the State Council emerged as the primary entities engaged in policymaking for HIV/AIDS in China.We identified four distinct stages in the evolution of these policies,culminating in a novel‘sun-shaped’HIV/AIDS prevention and control policy network model with an emphasis on‘knowledge,attitude,and practice’at its nucleus,which aligns with national conditions and societal progress.Furthermore,the focal groups of these policies have been dynamically refined and updated over time.Conclusions:Our findings introduce a‘sunshaped’HIV/AIDS prevention and control policy network model specific to China.We observed a conceptual policy shift towards prioritizing overall human health rather than confining the focus to disease treatment.Additionally,in light of China’s growing elderly population,the imperative to address HIV/AIDS prevention and control among older adults is an issue that warrants increased attention.

关键词： prevention HIV/AIDS policy