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Improved genome annotation of Brassica oleracea highlights the importance of alternative splicing

Horticultural Plant Journal 2024年第4期10卷 961-970页

作者： Yinqing Yang lei Zhang Qi Tang Lingkui Zhang Xing Li Shumin Chen Kang Zhang Ying Li Xilin hou Feng Cheng State Key Laboratory of Crop Genetics and Germplasm Enhancement College of HorticultureNanjing Agricultural UniversityNanjing 210095China State Key Laboratory of Vegetable Biobreeding Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural AffairsSino-Dutch Joint Laboratory of Horticultural GenomicsBeijing 100081China

Brassica oleracea has been developed into many important crops,including cabbage,kale,cauliflower,broccoli and so *** genome and gene annotation of cabbage(cultivar JZS),a representative morphotype of ***,has been widely used as a common reference in biological *** its genome assembly has been updated twice,the current gene annotation still lacks information on untranslated regions(UTRs)and alternative splicing(AS).Here,we constructed a high-quality gene annotation(JZSv3)using a full-length transcriptome acquired by nanopore sequencing,yielding a total of 59452 genes and 75684 ***,we re-analyzed the previously reported transcriptome data related to the development of different tissues and cold response using JZSv3 as a reference,and found that 3843 out of 11908 differentially expressed genes(DEGs)underwent AS during the development of different tissues and 309 out of 903 cold-related genes underwent AS in response to cold ***,we also identified many AS genes,including BolLHCB5 and BolHSP70,that displayed distinct expression patterns within variant transcripts of the same gene,highlighting the importance of JZSv3 as a pivotal reference for AS ***,JZSv3 provides a valuable resource for exploring gene function,especially for obtaining a deeper understanding of AS regulation mechanisms.

关键词： Brassica oleracea Oxford nanopore technologies Gene annotation Alternative splicing

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High-static-low-dynamic stiffness isolator based on an electromagnetic negative stiffness spring with long linear stroke

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Science China(Technological Sciences) 2024年第3期67卷 740-752页

作者： YUAN ShuJin WU YaJun SONG DaiPing PU HuaYan MOU LiSheng hou lei ZHAO Jinglei LI XuePing LUO Jun WU Jie HUANG XiaoXu State Key Laboratory of Mechanical Transmissions Chongqing UniversityChongqing 400044China China Aerodynamics Research and Development Center Mianyang 621000China College of Materials Science and Engineering Chongqing UniversityChongqing 400044China

Negative stiffness mechanisms can improve low-frequency vibration isolation performance and have been widely used in the vibration isolation of precision equipment. However, the negative stiffness mechanism usually introduces a nonlinear stiffness,resulting in a nonlinear response and worsening the vibration isolation performance, especially under large amplitude *** this paper, an electromagnetic spring with linear negative stiffness(ESLNS) is proposed, in which the antagonistic ampere forces of the energized coils are used to generate negative stiffness within a long linear stroke. The magnetic field distribution is improved through the design of the magnetic circuit, thereby increasing the stiffness generation efficiency. The stiffness can be adjusted bidirectionally by current within the range of positive and negative stiffness. An electromagnetic stiffness model was established based on the equivalent magnetic circuit method. Experimental measurements verified the accuracy of the model and proved the linearity of the electromagnetic spring. A vibration isolator with high static and low dynamic stiffness(HSLDS) based on the ESLNS is designed and tested. The experimental results prove that the introduction of the ESLNS can effectively expand the isolation frequency band without changing the equilibrium position. Moreover, the vibration isolator with ESLNS does not produce nonlinear response. The proposed electromagnetic spring with linear negative stiffness extends the application range of HSLDS isolators to a large amplitude vibration environment.

关键词： linear negative stiffness mechanism low-frequency vibration isolation electromagnetic spring vibration control

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Inhibition of the cGAS–STING pathway:contributing to the treatment of cerebral ischemia-reperfusion injury

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Neural Regeneration Research 2025年第7期20卷 1900-1918页

作者： Hang Yang Yulei Xia Yue Ma Mingtong gao Shuai hou Shanshan Xu Yanqiang Wang School of Clinical Medicine Shandong Second Medical UniversityWeifangShandong ProvinceChina Department of Emergency The Affiliated Hospital of Weifang Medical UniversityWeifangShandong ProvinceChina Department of Neurology II The Affiliated Hospital of Weifang Medical UniversityWeifangShandong ProvinceChina

The cGAS–STING pathway plays an important role in ischemia-reperfusion injury in the heart,liver,brain,and kidney,but its role and mechanisms in cerebral ischemia-reperfusion injury have not been systematically ***,we outline the components of the cGAS–STING pathway and then analyze its role in autophagy,ferroptosis,cellular pyroptosis,disequilibrium of calcium homeostasis,inflammatory responses,disruption of the blood–brain barrier,microglia transformation,and complement system activation following cerebral ischemia-reperfusion *** further analyze the value of cGAS–STING pathway inhibitors in the treatment of cerebral ischemia-reperfusion injury and conclude that the pathway can regulate cerebral ischemia-reperfusion injury through multiple *** of the cGAS–STING pathway may be helpful in the treatment of cerebral ischemia-reperfusion injury.

关键词： calcium homeostasis cellular autophagy cerebral ischemia-reperfusion injury cGAS–STING pathway ferroptosis gut–brain–microbiota axis inflammatory light chain 3 microglial cells Syntaxin-17 protein

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Sahara’s surface transformation forced an abrupt hydroclimate decline and Neolithic culture transition in northern China

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The Innovation 2024年第1期5卷 70-79页

作者： Yandong hou Hao Long Sumiko Tsukamoto Zhengyao Lu Jie Chen Daniel EIbarra Toru Tamura Qiong Zhang Weiyi Sun Jingran Zhang lei gao Manfred Frechen Ji Shen State Key Laboratory of Lake Science and Environment Nanjing Institute of Geography and LimnologyChinese Academy of Sciences(NIGLAS)Nanjing 210008China leibniz Institute for Applied Geophysics(LIAG) Department of Geochronology30655 HannoverGermany Department of Physical Geography and Ecosystem Science Lund University22100 LundSweden Key Laboratory of Western China’s Environmental Systems(Ministry of Education) College of Earth and Environmental SciencesLanzhou UniversityLanzhou 730000China College of Atmospheric Sciences Lanzhou UniversityLanzhou 730000China Institute at Brown for Environment and Society and Department of Earth Environmentaland Planetary SciencesBrown UniversityProvidenceRI 02912USA Geological Survey of Japan National Institute of Advanced Industrial Science and TechnologyTsukubaIbaraki 305-8567Japan Graduate School of Frontier Sciences University of TokyoKashiwaChiba 277-8561Japan Department of Physical Geography and Bolin Centre for Climate Research Stockholm University10691 StockholmSweden School of Geography Nanjing Normal UniversityJiangsu Centre for Collaborative Innovation in Geographical Information Resource Development and ApplicationKey Laboratory of Virtual Geographic EnvironmentMinistry of Education of ChinaNanjing 210023China School of Geography and Ocean Science Nanjing UniversityNanjing 210023China

The remote forcing from land surface changes in the Sahara is hypothesized to play a pivotal role in modulating the intensity of the East Asian summer monsoon(EASM)through ocean-atmospheric *** modulation has far-reaching consequences,particularly in facilitating societal shifts documented in northern ***,we present a well-dated lake-level record from the Daihai Lake Basin in northern China,providing quantitative assessments of Holocene monsoonal precipitation and the consequent migrations of the northern boundary of the *** reconstruction,informed by a water-and-energy balance model,indicates that annual precipitation reached700 mm during 8–5 ka,followed by a rapid decline to tween 5 and 4 *** shift coherently aligns with a signi550 mm be-ficant300 km northwestward movement of the EASM northern boundary during the Middle Holocene(MH),in contrast to its current *** findings underscore that these changes cannot be entirely attributed to orbital forcing,as corroborated by simulation *** model simulations deployed in our study suggest that the presence of the Green Sahara during the MH significantly strengthened the EASM and led to a northward shift of the monsoon rainfall ***,the Sahara’s reversion to a desert landscape in the late Holocene was accompanied by a corresponding southward retraction of monsoon *** dramatic hydroclimate changes during5–4 ka likely triggered or at least contributed to a shift in Neolithic cultures and societal transformation in northern *** decreasing agricultural productivity,communities transitioned from millet farming to a mixed rainfed agriculture and animal husbandry ***,our findings elucidate not only the variability of the EASM but also the profound implications of a remote forcing,such as surface transformations of the Sahara,on climatic changes and cultural evolution in northern China.

关键词： Holocene monsoon abrupt

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Transcriptomic and metabolomic analysis provides insights into lignin biosynthesis and accumulation and differences in lodging resistance in hybrid wheat

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Journal of Integrative Agriculture 2024年第4期23卷 1105-1117页

作者： Weibing Yang Shengquan Zhang Qiling hou Jiangang gao Hanxia Wang Xianchao Chen Xiangzheng Liao Fengting Zhang Changping Zhao Zhilie Qin Institute of Hybrid Wheat Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China Beijing Municipal Key Laboratory of Molecular Genetics of Hybrid Wheat Beijing 100097China

The use of hybrid wheat is one way to improve the yield in the ***,greater plant heights increase lodging risk to some *** this study,two hybrid combinations with differences in lodging resistance were used to analyze the stem-related traits during the filling stage,and to investigate the mechanism of the difference in lodging resistance by analyzing lignin synthesis of the basal second internode(BSI).The stem-related traits such as the breaking strength,stem pole substantial degree(SPSD),and rind penetration strength(RPS),as well as the lignin content of the lodging-resistant combination(LRC),were significantly higher than those of the lodgingsensitive combination(LSC).The phenylpropanoid biosynthesis pathway was significantly and simultaneously enriched according to the transcriptomics and metabolomics analysis at the later filling stage.A total of 35 critical regulatory genes involved in the phenylpropanoid pathway were ***,42%of the identified genes were significantly and differentially expressed at the later grain-filling stage between the two combinations,among which more than 80%were strongly up-regulated at that stage in the LRC compared with *** the contrary,the LRC displayed lower contents of lignin intermediate metabolites than the *** results suggested that the key to the lodging resistance formation of LRC is largely the higher lignin synthesis at the later grain-filling ***,breeding strategies for synergistically improving plant height and lodging resistance of hybrid wheat were put forward by comparing the LRC with the conventional wheat applied in large areas.

关键词： gene expression lignin synthesis lodging-resistance hybrid wheat

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Experimental investigation of inflow-outflow asymmetry in induced-charge electro-osmosis

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Energy Storage and Saving 2024年第1期3卷 16-22页

作者： Lingqi Zhao Tianwei Lai Yingke gao Shaohang Yan Mingzhe Liu Yu hou State Key Laboratory of Multiphase Flow in Power Engineering Xi’an Jiaotong UniversityXi’an710049China MOE Key Laboratory of Cryogenic Technology and Equipment Xi’an Jiaotong UniversityXi’an710049China

Induced-charge electro-osmosis(ICEO)is a research hotspot in bioengineering and analytical ***-outflow asymmetry of ICEO was reported in the existing literatures,but systematic study on this phenomenon is *** this experimental study,we found that in strong electric fields,not only the velocity magnitude but also the vortex positions of ICEO are asymmetrical along the inflow and outflow directions because of the pronounced non-uniform surface electrokinetic *** the inflow and outflow directions,the amplitudes of velocities are unequal,ICEO maximum velocity positions change depending on the electric field intensity and sodium chloride(NaCl)***,the distances between vortex centers are *** NaCl solution concentration of 0.001 mol⋅L^(-1),the outflow velocity almost *** asymmetry rises with the increasing electric field *** new discoveries can direct the application of microscale devices.

关键词： Induced-charge electro-osmosis Micro-particle image velocimetry Inflow-outflow asymmetry

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Preparation of Laser Cladding Coating Undercooling Cu-based Alloy and Co on Non-equilibrium Solidification Structure

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Journal of Wuhan University of Technology(Materials Science) 2024年第2期39卷 463-472页

作者：田徐铭 CAO Shichao hou Kai hou Xiaopeng WANG Hongfu 张煜 Department of Outpatient Xinzhou People’s HospitalXinzhou 034099China School of Mechanical Engineering North University of ChinaTaiyuan 030051China Department of Neurosurgery Xinzhou People’s HospitalXinzhou 034099China Department of Orthopedics Xinzhou People’s HospitalXinzhou 034099China

The effect of the gradient content of Co element on the solidification process of Cu-based alloy under deep under cooling conditions was *** non-equilibrium solidification structure of the under cooled alloy samples were *** is found that the rapidly solidified alloy has undergone twice grain refinement during the undercooling *** and significance of the maximum undercooling refinement structure of Cu60Ni35Co5 at T=253 K were ***-density defects were observed,such as dislocations,stacking faults networks,and twinning *** standard FCC diffraction pattern represents that it is still a single-phase *** on the metallographic diagram,EBSD and TEM data analysis,it is illustrated that the occurrence of grain refinement under high undercooling is due to stress induced *** addition,the laser cladding technology is used to coat Co-based alloy(Stellite12) coating on 304 stainless steel substrate;the microstructure of the coating cross-section was *** was found that the microstructure of the cross-section is presented as columnar crystals,planar crystals,and disordered growth direction,so that the coating has better hardness and wear *** electrochemical corrosion of the substrate and coating,it can be seen that the Co and Cr elements present in the coating are more likely to form a dense passivation film,which improved the corrosion resistance of the coating.

关键词： non-equilibrium solidification structure undercooling recrystallization laser cladding coating

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Multicomponent Seismic Data Reconstruction via Anti-Aliasing Vector POCS Method

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Journal of Earth Science 2024年第4期35卷 1385-1392页

作者： Fan Li Jianjun gao Yun Wang Chunming Wang Sian hou School of Geophysics and Information Technology China University of GeosciencesBeijing 100083China Research Institute of Petroleum Exploration and Development Petro ChinaBeijing 100083China

0 INTRODUCTION Due to the ability to comprehensively utilize P and S wave information,multi-component seismic exploration has already played an important role in fracture detection,lithology prediction,fluid identification,etc.,and it has also become an important technology for complex reservoir exploration(Yuan et al.,2021;Stewart et al.,2003).

关键词： reservoir Seismic prediction

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The coupling control of biological precursors and environmental factors onβ-carotane enrichment in alkaline lacustrine source rocks:A case study from the Fengcheng formation in the western Junggar Basin,NW China

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Petroleum Science 2024年第2期21卷 836-854页

作者： Mao-Guo hou Ming Zha Hua Liu Hai-lei Liu Jiang-Xiu Qu Ablimit Imin Xiu-Jian Ding Zhong-Fa Jiang School of Geosciences China University of PetroleumQingdaoShandong266580China Research Institute of Exploration and Development Xinjiang Petroleum Administration BureauKaramayXinjiang834000China

The organic-rich mudstones and dolostones of the Permian Fengcheng Formation(Fm.)are typically alkaline lacustrine source rocks,which are typified by impressively abundantβ-*** β-carotane has been well acknowledged as an effective indicator of biological sources or depositional ***,the specific biological sources of β-carotane and the coupling control of biological sources and environmental factors on the enrichment of β-carotane in the Fengcheng *** *** on a comprehensive investigation of the bulk,molecular geochemistry,and organic petrology of sedimentary rocks and the biochemistry of phytoplankton in modern alkaline lakes,we proposed a new understanding of the biological precursors of β-carotane and elucidated the enrichment mechanism of β-carotane in the Fengcheng *** results show that the biological precursors crucially control the enrichment of β-carotane in the Fengcheng *** haloalkaliphilic cyanobacteria are the primary biological sources of β-carotane,which is suggested by a good positive correlation between the 2-methylhopane index,7-+8-methyl heptadecanes/C_(max),C_(29%),and β-carotane/C_(max)in sedimentary rocks and the predominance of cyanobacteria with abundantβ-carotene in modern alkaline *** enrichment of β-carotane requires the reducing condition,and the paleoredox state that affects the enrichment of β-carotane appears to have a *** paleoclimate conditions do not considerably impact the enrichment of β-carotane,but they have some influence on the water's paleosalinity by affecting evaporation and *** it does not directly affect the enrichment of β-carotane in the Fengcheng Fm.,paleosalinity does have an impact on the cyanobacterial precursor supply and the preservation conditions.

关键词： β-carotane enrichment Cyanobacterial input Environmental impact Alkaline lacustrine source rocks The Fengcheng formation

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Mitigating Lattice Distortion of High‑Voltage LiCoO_(2)via Core‑Shell Structure Induced by Cationic Heterogeneous Co‑Doping for Lithium‑Ion Batteries

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Nano-Micro Letters 2024年第3期16卷 169-182页

作者： Zezhou Lin Ke Fan Tiancheng Liu Zhihang Xu gao Chen Honglei Zhang Hao Li Xuyun Guo Xi Zhang Ye Zhu Peiyu hou Haitao Huang Department of Applied Physics and Research Institute for Smart Energy The Hong Kong Polytechnic UniversityHong KongPeople’s Republic of China Department of Applied Physics The Hong Kong Polytechnic UniversityHong KongPeople’s Republic of China Institute of Nanosurface Science and Engineering Guangdong Provincial Key Laboratory of Micro/Nano Optomechatronics EngineeringShenzhen UniversityShenzhenPeople’s Republic of China School of Physics and Technology University of JinanJinanShandongPeople’s Republic of China

Inactive elemental doping is commonly used to improve the structural stability of high-voltage layered transition-metal oxide ***,the one-step co-doping strategy usually results in small grain size since the low diffusivity ions such as Ti^(4+)will be concentrated on grain boundaries,which hinders the grain *** order to synthesize large single-crystal layered oxide cathodes,considering the different diffusivities of different dopant ions,we propose a simple two-step multi-element co-doping strategy to fabricate core–shell structured LiCoO_(2)(CS-LCO).In the current work,the high-diffusivity Al^(3+)/Mg^(2+)ions occupy the core of single-crystal grain while the low diffusivity Ti^(4+)ions enrich the shell *** Ti^(4+)-enriched shell layer(~12 nm)with Co/Ti substitution and stronger Ti–O bond gives rise to less oxygen ligand ***-situ XRD demonstrates the constrained contraction of c-axis lattice parameter and mitigated structural *** a high upper cut-off voltage of 4.6 V,the single-crystal CS-LCO maintains a reversible capacity of 159.8 mAh g^(−1)with a good retention of~89%after 300 cycles,and reaches a high specific capacity of 163.8 mAh g^(−1)at *** proposed strategy can be extended to other pairs of low-(Zr^(4+),Ta^(5+),and W6+,etc.)and high-diffusivity cations(Zn^(2+),Ni^(2+),and Fe^(3+),etc.)for rational design of advanced layered oxide core–shell structured cathodes for lithium-ion batteries.

关键词： Lithium-ion battery LiCoO_(2) Heterogeneous co-doping Core-shell structure High-voltage stability

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