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Establishment of human cerebral organoid systems to model early neural development and assess the central neurotoxicity of environmental toxins

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Neural Regeneration Research 2025年第1期20卷 242-252页

作者： Daiyu hu yuanqing Cao Chenglin Cai Guangming Wang Min Zhou Luying Peng yantao Fan Qiong Lai Zhengliang gao Fundamental Research Center Shanghai Yangzhi Rehabilitation Hospital(Shanghai Sunshine Rehabilitation Center)School of MedicineTongji UniversityShanghaiChina Institute of Geriatrics(Shanghai University) Affiliated Nantong Hospital of Shanghai University(The Sixth People’s Hospital of Nantong)Shanghai University School of MedicineNantongJiangsu ProvinceChina Shanghai Engineering Research Center of Organ Repair Shanghai University School of MedicineShanghaiChina Key Laboratory of Arrhythmias Ministry of EducationShanghai East HospitalSchool of MedicineTongji UniversityShanghaiChina

human brain development is a complex process,and animal models often have significant *** address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-like organoids,to more accurately model early.human brain development and *** enable more consistent and intuitive reproduction of early brain development,in this study,we incorporated forebrain organoid culture technology into the traditional unguided method of brain organoid *** involved embedding organoids in matrigel for only 7 days during the rapid expansion phase of the neural epithelium and then removing them from the matrigel for further cultivation,resulting in a new type of human brain organoid *** cerebral organoid system replicated the temporospatial characteristics of early.human brain development,including neuroepithelium derivation,neural progenitor cell production and maintenance,neuron differentiation and migration,and cortical layer patterning and formation,providing more consistent and reproducible organoids for developmental modeling and toxicology *** a proof of concept,we applied the heavy metal cadmium to this newly improved organoid system to test whether it could be used to evaluate the neurotoxicity of environmental *** organoids exposed to cadmium for 7 or 14 days manifested severe damage and abnormalities in their neurodevelopmental patterns,including bursts of cortical cell death and premature *** exposure caused progressive depletion of neural progenitor cells and loss of organoid integrity,accompanied by compensatory cell proliferation at ectopic *** convenience,flexibility,and controllability of this newly developed organoid platform make it a powerful and affordable alternative to animal models for use in neurodevelopmental,neurological,and neurotoxicological studies.

关键词： cadmium cell death cell proliferation cortical development environmental toxins neural progenitor cells neurogenesis neurotoxicology organoids stem cells

维普期刊数据库

Primary porcine hepatocytes with portal vein serum cultured on microcarriers or in spheroidal aggregates

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World Journal of Gastroenterology 2000年第3期6卷 365-370页

作者： gao y.hu hz Chen K yang JZ First Mil Med Univ Zhujiang Hosp Dept Hepatobiliary Surg Guangzhou 510282 Guangdong Provi Peoples R China

AIM To develop a culture mode providingdurable biomaterials with high yields andactivities used in bioartificial *** Hepatocytes were isolated from awhole pig liver by Seglen’s method of orthotopicperfusion with *** culture onmicrocarriers,primary porcine hepatocyteswere inoculated at a concentration of 5×10~7/mLinto the static culture systems containing 2 g/LCytodex-3,then supplemented with 100 mL/Lfetal calf serum(FCS)or 100 mL/L porcineportal vein serum(PPVS)*** aggregate culture hepatocytes wereinoculated into 100 mL siliconized flasks at aconcentration of 5.0×10~6/*** In culture on microcarriershepatocytes tended to aggregate on Cytodex-3obviously after being *** multi-cellular aggregated spheroids could be found inthe two systems 24 h-48 h after hepatocyteswere *** morphological charact-eristics and synthetic functions were maintainedfor 5 wk in FCS culture system and 8 wk in PPVSculture *** spheroidal aggregate cultureabout 80%-90% isolated hepatocytes becameaggregated spheroids 24h after cultured insuspension and mean diameter of the spheroidswas 100μ*** relationship among thehepatocytes resembled that in the liver in *** functions of albumin and urea of the spheroids were twice those of hepatocytescultured on *** As high-yields and high-activitymodes of culture on microcarriers or inspheroidal aggregate culture with portal veinserum are promising to provide biomaterials forbioartificial liver(BAL)efficiently.

关键词： porcine hepatocytes microcarriers cell culture spheroidal aggregate culture portal vein serum

Cultivation of human liver cell lines with microcarriers acting as biological materials of bioartificial liver

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World Journal of Gastroenterology 1999年第3期5卷 221-224页

作者： gao y Xu XP hu hz yang JZ Mil Med Univ 1st Dept Gen Surg Zhujiang Hosp Guangzhou 510282 Guangdong Prov Peoples R China

AIM To improve the Cultivation efficiency and yield of human liver cell line *** High--density cultivation of CL--l onmicrocarriers was carried out with periodicobservation of their growth and *** specific functions of human liver cell werealso *** Cells of CL-1 cell line grew well onmicrocarrier Cytodex--3 and on the 7th day thepeak was reached. The amount of CLI cells was2.13 x 10’ and the total amount of albuminsynthesis reached 71 .23 ig, urea synthesis23 .32 mg and diazepam transformation 619 .7 ugrespectively. The yield of CL-l on microcarrierswas 49.3 times that of conventional *** amounts of albumin synthesis, ureasynthesis and diazepam transformation were39.8 times, 41 .6 times and 33.3 times those ofconventional Cultivation, *** The human liver cell line CL--1 canbe Cultivated to a high density with Cytodex--3and has better biological functions. High-densityCultivation of CLI on microcarriers can act asthe biological material of bioartificial liver.

关键词： cell Figure

Nanotechnology Powered CRISPR-Cas Systems for Point of Care Diagnosis and Therapeutic

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Research 2023年第3期2022卷 155-156页

作者： Chen-zhong Li Tony y.hu Center for Cellular and Molecular Diagnostics Department of Biochemistry and Molecular BiologyTulane University School of Medicine1324Tulane Ave.New OrleansLA70112US

Inspired by the successful application of Cas9 gene editing for“genome surgery,”we are looking back at what has been accomplished in the ten years of CRISPR *** effort is now focused on how to utilize the unique functions of CRISPR-Cas systems for molecular diagnostics and the next generation of therapeutic ***-Cas systems have two major advantages for gene editing that render them attractive for these new applications,specifically the ability to easily control their target recognition specificity and to couple this specificity to a targeted or secondary cleavage ***,despite the great potential of CRISPR/Cas for both gene editing and diagnosis,its medical applications have yet to be fully realized due factors that limit their delivery or utility for point-of-care(POC)*** this editorial note,we discuss how nanotechnology can address challenges associated with new CRISPR applications and what nanotechnology-specific advances are needed to circumvent remaining barriers that limit the development or use of new CRISPR/CAS-based genome editing and diagnostic approaches.

关键词： limit specificity utilize

Strategies for advanced personalized tuberculosis diagnosis:Current technologies and clinical approaches

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Precision Clinical Medicine 2021年第1期4卷 35-44页

作者： Xuerong Chen Tony y.hu Department of Respiratory and Critical Care Medicine West China HospitalSichuan UniversityChengdu 610041China Center for Cellular and Molecular Diagnostics School of MedicineTulane UniversityNew OrleansLA 70112USA

Diagnosis of tuberculosis can be difficult as advances in molecular diagnosis approaches(especially nanoparticles combined with high-throughput mass spectrometry for detecting mycobacteria peptide)and personalized medicine result in many changes to the diagnostic *** review will address issues concerning novel technologies from bench to bed and new strategies for personalized tuberculosis diagnosis.

关键词： tuberculosis molecular diagnosis personalized medicine

Domain generalization with semi-supervised learning for people-centric activity recognition

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Science China(Information Sciences) 2025年第1期68卷 171-188页

作者： Jing LIU Wei Zhu Di LI Xing hu Liang SONG Academy for Engineering & Technology Fudan University Shanghai East-bund Research Institute on Networking Systems of AI School of Optoelectronic Information and Computer Engineering University of Shanghai for Science & Technology

People-centric activity recognition is one of the most critical technologies in a wide range of real-world applications,including intelligent transportation systems, healthcare services, and brain-computer interfaces. Large-scale data collection and annotation make the application of machine learning algorithms prohibitively expensive when adapting to new tasks. One way of circumventing this limitation is to train the model in a semi-supervised learning manner that utilizes a percentage of unlabeled data to reduce the labeling burden in prediction tasks. Despite their appeal, these models often assume that labeled and unlabeled data come from similar distributions, which leads to the domain shift problem caused by the presence of distribution gaps. To address these limitations, we propose herein a novel method for people-centric activity recognition,called domain generalization with semi-supervised learning(DGSSL), that effectively enhances the representation learning and domain alignment capabilities of a model. We first design a new autoregressive discriminator for adversarial training between unlabeled and labeled source domains, extracting domain-specific features to reduce the distribution gaps. Second, we introduce two reconstruction tasks to capture the task-specific features to avoid losing information related to representation learning while maintaining task-specific consistency. Finally, benefiting from the collaborative optimization of these two tasks, the model can accurately predict both the domain and category labels of the source domains for the classification task. We conduct extensive experiments on three real-world sensing datasets. The experimental results show that DGSSL surpasses the three state-of-the-art methods with better performance and generalization.

关键词： activity recognition deep learning domain generalization semi-supervised learning adversarial training

同方期刊数据库

Channel pruning on frequency response

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Science China(Information Sciences) 2025年第1期68卷 159-170页

作者： Hang LIN yifan PENG Lin BIE Chenggang yAN Xibin ZHAO yue gao School of Software Tsinghua University School of Automation Hangzhou Dianzi University

Network pruning has a significant role in reducing network parameters and accelerating the inference time of the network. Some existing methods prune the network based on the frequency of the data, and finally obtain a sub-network with high accuracy. However, according to our experimental analysis, different frequencies of information in the data contribute differently to the accuracy of the model, and using this information directly for pruning without making a selection will lead to incorrect results. We believe that pruning should retain the convolutional kernels in the network that process important information, while those kernels that process unimportant information should be removed. In this paper, we first investigate the meaning of each frequency band information in the spectrum and their contribution to the prediction accuracy of the network,and according to these results, we propose a new pruning method based on frequency response(PFR). Our PFR finds and removes the convolutional kernels in the network that specialize in processing unimportant information, resulting in a compact neural network model. PFR obtains significant experimental results on different datasets, for example, a 56.0% raduction of float points operations(FLOPs) on Res Net-50 and only 0.37% of Top-1 accuracy degradation on the Image Net dataset.

关键词： deep learning model compression filter pruning channel pruning frequency response

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Growth behavior and electronic regulation of Pt on various Mo-based supports for hydrogen evolution electrocatalysis

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Science China Chemistry 2025年

作者： Lulu Chen yichao huang Jiajun Wang Meihong Liao Zhiyuan Liu Meiqi Liu Hanqing Cai Lin Wang Lidong gao Dawei hu Lianming Zhao Ning Pu Zhuangjun Fan State Key Laboratory of Chemical Safety School of Materials Science and Engineering China University of Petroleum (East China) School of Mechanical and Electronic Engineering Qingdao Binhai University Qingdao Chuangqi New Energy Catalysis Technology Co.Ltd. Sinopec Research Institute of Petroleum Processing Co.Ltd. Institute of Energy Hefei Comprehensive National Science Center

Insight into the growth behavior and electronic regulation of platinum （Pt） on various transition metal supports is paramount in developing high-performing electrocatalysts for hydrogen evolution reactions （HER）.Herein,we studied the influence of molybdenum-based supports （MoX,X=C,N,P,and S） on the growth behavior and electronic regulation of *** found that the formation energy variations between Pt single atom and clusters on MoX supports play a pivotal role in the growth behavior of ***,the electronic regulation of Pt induced by metal-support interaction may reflect the valence changes of Pt in Pt-MoX/*** Pt-MoC/C catalyst with a moderate valence state of Pt exhibits the best HER activity with an overpotential of 12.0 mV at 10 mA cm-2and a mass activity of 27.1 A mgPt-1,12.3 times as high as that of commercial 20 wt% Pt/*** work provides constructive guidance for the design of high-performance HER catalysts.

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Clinical Peptidomics: Advances in Instrumentation, Analyses, and Applications

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Biomedical Engineering Frontiers 2023年第1期4卷 116-132页

作者： Lin Li Jing Wu Christopher JLyon Li Jiang Tony y.hu Center for Cellular and Molecular Diagnostics Department of Biochemistry and Molecular BiologySchool of MedicineTulane UniversityNew OrleansLAUSA Department of Laboratory Medicine and Sichuan Provincial Key Laboratory for human Disease Gene Study Sichuan Academy of Medical Sciences and Sichuan Provincial People’s HospitalChengduChina Department of Clinical Laboratory Third Central Hospital of TianjinTianjin Institute of Hepatobiliary DiseaseTianjin Key Laboratory of Artificial CellArtificial Cell Engineering Technology Research Center of Public Health MinistryTianjinChina Department of Biomedical Engineering School of Science and EngineeringTulane UniversityNew OrleansLAUSA

Extensive effort has been devoted to the discovery,development,and validation of biomarkers for early disease diagnosis and prognosis as well as rapid evaluation of the response to therapeutic *** and transcriptomic profiling are well-established means to identify disease-associated ***,analysis of disease-associated peptidomes can also identify novel peptide biomarkers or signatures that provide sensitive and specific diagnostic and prognostic information for specific malignant,chronic,and infectious *** evidence also suggests that peptidomic changes in liquid biopsies may more effectively detect changes in disease pathophysiology than other molecular *** gained from peptide-based diagnostic,therapeutic,and imaging approaches has led to promising new theranostic applications that can increase their bioavailability in target tissues at reduced doses to decrease side effects and improve treatment ***,despite major advances,multiple factors can still affect the utility of peptidomic *** review summarizes several remaining challenges that affect peptide biomarker discovery and their use as diagnostics,with a focus on technological advances that can improve the detection,identification,and monitoring of peptide biomarkers for personalized medicine.

关键词： doses gained devoted