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Identifying key genes associated with Hirschsprung's disease based on bioinformatics analysis of RNA-sequencing data

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World Journal of Pediatrics 2017年第3期13卷 267-273页

作者： Wei-Kang Pan ya-Fei Zhang Hui Yu ya gao Bai-Jun Zheng Peng Li Chong Xie Xin Ge Department of Pediatric Surgery The Second Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710004China Department of Endoscopy Shaanxi Nuclear Industry 215 HospitalXianyang 712000China

Background:Hirschsprung's disease (HSCR) is a type of megacolon induced by deficiency or dysfunction of ganglion cells in the distal intestine and is associated with developmental disorders of the enteric nervous *** explore the mechanisms of HSCR,we analyzed the RNA-sequencing data of the expansion and the narrow segments of colon tissues separated from children with ***:RNA-sequencing of the expansion segments and the narrow segments of colon tissues isolated from children with HSCR was *** differentially expressed genes (DEGs) were identified using the edgeR package in R,functional and pathway enrichment analyses of DEGs were carried out using DAVID *** further screen the key genes,protein-protein interaction (PPI) network and module analyses were conducted separately using Cytoscape ***:A total of 117 DEGs were identified in the expansion segment samples,including 47 up-regulated and 70 down-regulated *** enrichment analysis suggested that FOS and DUSP1 were implicated in response to endogenous *** the PPI network analysis,FOS (degree=20),EGR1 (degree=16),ATF3 (degree=9),NOS1 (degree=8),CCL5 (degree=8),DUSP1 (degree=7),CXCL3 (degree=6),VIP (degree=6),FOSB (degree=5),and NOS2 (degree=4) had higher degrees,which could interact with other *** addition,two significant modules (module 1 and module 2) were identified from the PPI ***:Several genes (including FOS,EGR1,ATF3,NOS1,CCL5,DUSP1,CXCL3,VIP,FOSB,and NOS2) might be involved in the development of HSCR through their effect on the nervous system.

关键词： differentially expressed genes functional and pathway enrichment analysis Hirschsprung's disease protein-protein interaction network

Second harmonic magnetoacoustic responses of magnetic nanoparticles in magnetoacoustic tomography with magnetic induction

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Chinese Physics B 2020年第3期29卷 259-264页

作者： Gepu Guo ya gao Yuzhi Li Qingyu Ma Juan Tu Dong Zhang School of Physics and Technology Nanjing Normal UniversityNanjing 210023China Institute of Acoustics Nanjing UniversityNanjing 210093China

Due to the unique magnetic, mechanical and thermal properties, magnetic nanoparticles(MNPs) have comprehensive applications as the contrast and therapeutic agents in biomedical imaging and magnetic hyperthermia. The linear and nonlinear magnetoacoustic responses determined by the magnetic properties of MNPs have attracted more and more attention in biomedical engineering. By considering the relaxation time of MNPs, we derive the formulae of second harmonic magnetoacoustic responses(2H-MARs) for a cylindrical MNP solution model based on the mechanical oscillations of MNPs in magnetoacoustic tomography with magnetic induction(MAT-MI). It is proved that only the second harmonic magnetoacoustic oscillations can be generated by MNPs under an alternating magnetic excitation. The acoustic pressure of the 2H-MAR is proportional to the square of the magnetic field intensity and exhibits a linear increase with the concentration of MNPs. Numerical simulations of the 2H-MAR are confirmed by the experimental measurements for various magnetic field intensities and solution concentrations using a laser vibrometer. The favorable results demonstrate the feasibility of the harmonic measurements without the fundamental interference of the electromagnetic excitation, and suggest a new harmonic imaging strategy of MAT-MI for MNPs with enhanced spatial resolution and improved signal-to-noise ratio in biomedical applications.

关键词： magnetoacoustic tomography with magnetic induction second harmonic magnetoacoustic responses magnetic nanoparticles magnetic force mechanical oscillations

Investigation of the spatiotemporal variation and influencing factors on fine particulate matter and carbon monoxide concentrations near a road intersection

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Frontiers of Earth Science 2017年第1期11卷 63-75页

作者： Zhanyong WANG Qing-Chang LU Hong-Di HE Dongsheng WANG ya gao Zhong-Ren PENG Center for ITS and UAV Applications Research State Key Laboratory of Ocean Engineering School of Naval Architecture Ocean & Civil Engineering Shanghai Jiao Tong University Shanghai 200240 China Logistics Research Center Shanghai Maritime University Shanghai 201306 China Department of Urban and Regional Planning University of Florida Gainesville FL 32611-5706 USA

The minute-scale variations of fine particulate matter （PM2.5） and carbon monoxide （CO） concentrations near a road intersection in Shanghai, China were investigated to identify the influencing factors at three traffic periods. Measurement results demonstrate a synchronous variation of pollutant concentrations at the roadside and setbacks, and the average concentration of PM2.5 at the roadside is 7% （44% for CO） higher than that ofsethacks within 500 m of the intersection. The pollution level at traffic peak periods is found to be higher than that of off-peak periods, and the morning peak period is found to be the most polluted due to a large amount of diesel vehicles and unfavorable dispersion conditions. Partial least square regressions were constructed for influencing factors and setback pollutant concentrations, and results indicate that meteorological factors are the most significant, followed by setback distance from the intersection and traffic factors. CO is found to be sensitive to distance from the traffic source and vehicle type, and highly dependent on local traffic conditions, whereas PM2.5 originates more from other sources and background levels. These findings demonstrate the importance of localized factors in understanding spatiotemporal patterns of air pollution at intersections, and support decision makers in roadside pollution management and control.

关键词： traffic-related pollutants fine-scale variation distance gradient meteorology road intersection

A glutathione-triggered precision explosive system for improving tumor chemosensitivity

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Nano Research 2021年第7期14卷 2372-2382页

作者： Yuanyuan Nie Yurui Xu ya gao Jielei He Lei Sun Jianmei Chen Yushuang Cui Haixiong Ge Xinghai Ning National Laboratory of Solid State Microstructures Collaborative Innovation Center of Advanced MicrostructuresChemistry and Biomedicine Innovation CenterCollege of Engineering and Applied SciencesJiangsu Key Laboratory of Artificial Functional MaterialsNanjing UniversityNanjing 210093China

Stimuli-responsive delivery systems hold promise in cancer ***,their application potential has been limited due to undesirable drug leaking during blood circulation and inefficient therapeutic efficacy in tumors,resulting in undesirable therapeutic ***,we have developed a novel redox-sensitive pegylated phospholipid,termed as DOPE-SS-PEG,which can form a glutathione(GSH)-triggered precision explosive system(GPS)for simultaneously improving circulation stability,tumor specificity,and chemosensitivity,leading to explosive anticancer *** is constructed of liposomal doxorubicin(DOX)functionalized with DOPE-SS-PEG and MnO_(2) nanoparticles,which can protect liposome structure in the presence of serum GSH(20μM),whereas converts to cationic liposome in response to intracellular GSH(10 mM),thereby enhancing circulation stability,tumor specificity,endosomal escape,and cytoplasmic ***,GPS can not only generate oxygen to relieve hypoxia and consequently enhance chemosensitivity,but quench GSH antioxidability to elevate the accruement of intracellular reactive oxygen species(ROS),leading to an explosion of oxidative stress induced cell ***,in vivo studies show that GPS selectively accumulates in tumor tissues,effectively inhibits tumor growth,exhibits minimal systemic adverse effects,and consequently prolongs the survival time of tumor-bearing ***,GPS is a unique stimuli-responsive treatment with programmed and on-demand drug delivery,as well as explosive therapeutic efficacy,and provides an intelligent anticancer treatment.

关键词： glutathione-responsive serum stability selective intracellular release synergistic effects explosive reactive oxygen species(ROS)production enhanced anticancer effects

Boosting solar hydrogen production via electrostatic interaction mediated ***-TiO_(2−x)biohybrid system

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Nano Research 2024年第6期17卷 5390-5398页

作者： Xingxing Lv Weicheng Huang ya gao Rui Chen Xiaowei Chen Danqing Liu Ling Weng Liangcan He Shaoqin Liu School of Material Science and Chemical Engineering Harbin University of Science and TechnologyHarbin 150040China School of Medicine and Health and Key Laboratory of Micro-systems and Micro-structures Manufacturing of Ministry of EducationHarbin Institute of TechnologyHarbin 150001China Department of Physics School of ScienceJimei UniversityXiamen 361021China Zhengzhou Research Institute Harbin Institute of TechnologyZhengzhou 450046China

Hydrogen is garnering growing attention as a green energy source with zero carbon ***,most hydrogen production technologies still rely on the consumption of fossil fuels and are therefore *** has driven the search for more environmentally friendly methods of hydrogen *** this work,we present an innovative approach to enhance hydrogen generation via electrostatic interaction in the Escherichia coli and defective titanium dioxide(TiO_(2−x))*** method involves narrowing the forbidden bandwidth of TiO2 while introducing defect bands into its conduction band to facilitate visible light absorption and efficient charge *** biohybrid system,consisting of *** and TiO_(2−x),demonstrates a remarkable capability to produce 1.25 mmol of hydrogen within a 3-h timeframe under visible light *** accomplishment signifies a 3.31-fold rise in hydrogen production in comparison to ***,signifying a substantial enhancement in hydrogen production ***,we delve into the alterations in biological metabolites associated with hydrogen production and the changes in electron transfer in different biohybrid *** provides valuable insights into the understanding of the intrinsic mechanisms that drive the *** work introduces a novel and promising avenue for achieving this exciting goal.

关键词： Escherichia coli TiO_(2−x)nanoparticles biohybrid system hydrogen production

Influence of sea-land breeze on the formation and dissipation of severe dense fog and its burst reinforcement in the Yellow Sea coastal area,China

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Science China Earth Sciences 2024年第2期67卷 432-449页

作者： ya gao Duanyang LIU Shuqi yaN Wenjun ZHOU Hongbin WANG Fan ZU Qin MEI Chuanxiang YI Ye SHENG Key Laboratory of Transportation Meteorology of China Meteorological Administration Nanjing210041China yancheng Meteorological Bureau Yancheng224000China Nanjing Joint Institute for Atmospheric Sciences Nanjing210041China Key Laboratory of Disaster Weather Chinese Academy of Meteorological SciencesBeijing100086China

Based on the global reanalysis data of the National Centers for Environmental Prediction(NCEP)/National Center for Atmospheric Research,the surface meteorological observation data,sounding data and satellite observation data,this paper comprehensively analyzes the evolution process and formation mechanism of a persistent severe dense fog process occurred on February 15–17,2015 in yancheng,eastern *** the numerical simulation experiment of Weather Research and Forecast(WRF)model,we further analyze the impact of sea-land breeze on the formation and burst reinforcement of *** show that the precipitation caused by the southwesterly airflow in front of the upper-level trough and the low-pressure inverted trough are conducive to the formation of early rain fog,while the nighttime clear radiance under the control of surface cold high and the infiltration of weak cold advection are conducive to the formation and development of later radiation-advection *** WRF model simulates the fog evolution process,which is basically consistent with the actual fog area,and the simulation results are credible to a certain *** simulation results show that the establishment of sea breeze has an advection cooling effect on the near surface layer,which is conducive to the formation and development of the inversion layer on the near surface,providing stable stratification conditions for the formation and burst reinforcement of *** one hand,the strengthening of sea breeze circulation can continuously transport water vapor to the study *** the other,the occurrence of ultra-low level jet is favorable for the accumulation of low-level water *** the same time,the inversion intensity further strengthens,which is in favor of the burst reinforcement and long-term maintenance of fog.

关键词： Radiation-advection fog Sea-land breeze Atmospheric boundary layer Fog burst reinforcement WRF model

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A STABILITY RESULT FOR TRANSLATINGSPACELIKE GRAPHS IN LORENTZ MANIFOLDS

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Acta Mathematica Scientia 2024年第2期44卷 474-483页

作者：高雅毛井吴传喜 Faculty of Mathematics and Statistics Key Laboratory of Applied Mathematics of Hubei ProvinceHubei UniversityWuhan430062China

In this paper,we investigate spacelike graphs defined over a domain Ω⊂M^(n) in the Lorentz manifold M^(n)×ℝ with the metric−ds^(2)+σ,where M^(n) is a complete Riemannian n-manifold with the metricσ,Ωhas piecewise smooth boundary,and ℝ denotes the Euclidean *** prove an interesting stability result for translating spacelike graphs in M^(n)×ℝ under a conformal transformation.

关键词： mean curvature flow spacelike graphs translating spacelike graphs maximal spacelike graphs constant mean curvature Lorentz manifolds

All-trans retinoic acid increases ARPE-19 cell apoptosis via activation of reactive oxygen species and endoplasmic reticulum stress pathways

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International Journal of Ophthalmology(English edition) 2020年第9期13卷 1345-1350页

作者： Juan Wu Zhen-ya gao Dong-Mei Cui Hong-Hui Li Jun-Wen Zeng State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060Guangdong ProvinceChina Xuchang University School of MedicineXuchang 461000Henan ProvinceChina Chengdu Aier Eye Hospital Chengdu 610000Sichuan ProvinceChina

AIM:To explore the apoptosis of ARPE-19 cells after the treatment with different doses of all-trans-retinoic acid(ATRA).METHODS:ARPE-19 cells were used in the in-vitro *** cytometry assay was employed to evaluate the level of reactive oxygen species(ROS)and *** effects of ATRA(concentrations from 2.5 to 20μmol/L)on the expression of endoplasmic reticulum stress(ERS)markers in vitro were evaluated by Western blot and realtime quantitative polymerase chain reaction(qRT-PCR)*** contribution of ROS and ERS-induced apoptosis in vitro was determined by using N-acetyl-L-cysteine(NAC)and Salubrinal,an antagonist of NAC and ERS,***:Flow cytometry showed that ATRA significantly increased ARPE-19 cell apoptosis and ROS levels in each group(F=86.39,P<0.001;F=116.839.P<0.001).Western blot and qRT-PCR revealed that levels of CHOP and BIP were elevated in a concentration-dependent pattern after the cells were incubated with ATRA(2.5-20μmol/L).The upregulation of VEGF-A and CHOP induced by ATRA could be inhibited by NAC(antioxidant)and Salubrinal(ERS inhibitor)in ***:ATRA induces the apoptosis of ARPE-19 cells via activated ROS and ERS signaling pathways.

关键词： all-trans-retinoic acid retinal pigment epithelium apoptosis reactive oxygen species endoplasmic reticulum stress

Photocatalytic Cr(Ⅵ)reduction over MIL-88A(Fe)on polyurethane sponge: From batch to continuous-flow operation

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Chinese Chemical Letters 2023年第7期34卷 236-239页

作者： Xiao-Hong Yi ya gao Chong-Chen Wang Yu-Hang Li Hong-Yu Chu Peng Wang Beijing Key Laboratory of Functional Materials for Building Structure and Environment Remediation School of Environment and Energy Engineering.Beijing University of Civil Engineering and ArchitectureBeijing 100044China Beijing Energy Conservation&Sustainable Urban and Rural Development Provincial and Ministry Co-construction Collaboration Innovation Center Beijing University of Civil Engineering and ArchitectureBeijing100044China

MIL-88A(Fe)@sponge(MS) was synthesized by a dip-coating method, which displayed efficient photocatalytic Cr(Ⅵ) reduction efficiency under both low power LED UV light and real solar light irradiation. It was observed that MS(0.2 g/L) could remove 100% Cr(Ⅵ)(10 mg/L) by adding 0.4 mmol/L tartaric acid(TA) without adjusting pH(pH 5.05) within 6.0 min and 3.0 min under UV light and real solar light irradiation, respectively. Besides, the photo-induced e-and radicals(O_(2)^(·-) and CO_(2)^(·-)) were found to play the momentous roles in the MS/TA/UVL/Cr(Ⅵ) system by the scavenger experiments and electron spin resonance(ESR) tests. MS was also filled into a fixed-bed reactor to test the possibility of long-term Cr(Ⅵ)reduction operation in TA/UVL system. As expected, the results revealed that MS could still maintain 100% activity up to 60 h. These results demonstrated that MIL-88A(Fe) might be the potentially efficient catalyst for large-scale wastewater treatment in the near future.

关键词： MIL-88A(Fe) Polyurethane sponge Cr(Ⅵ)reduction Tartaric acid Continuous operation