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ABF2, ABF3, and ABF4 Promote ABA-Mediated Chlorophyll Degradation and Leaf Senescence by Transcriptional Activation of Chlorophyll Catabolic Genes and Senescence-Associated Genes in Arabidopsis

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Molecular Plant 2016年第9期9卷 1272-1285页

作者： Shan gao jiong gao Xiaoyu Zhu Yi Song Zhongpeng Li Guodong Ren Xin Zhou Benke Kuai State Key Laboratory of Genetic Engineering School of Life Sciences Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering Institute of Biodiversity ScienceFudan University Shanghai 200438 China These authors contributed equally to this article.

Chlorophyll （Chl） degradation is an integral process of leaf senescence, and NYE1/SGR1 has been demonstrated as a key regulator of Chl catabolism in diverse plant species. In this study, using yeast one-hybrid screening, we identified three abscisic acid （ABA）-responsive element （ABRE）-binding transcription factors, ABF2 （AREB1）, ABF3, and ABF4 （AREB2）, as the putative binding proteins of the NYE1 promoter. Through the transactivation analysis, electrophoretic mobility shift assay, and chromatin immunoprecipitation, we demonstrated that ABF2, ABF3, and ABF4 directly bound to and activated the NYE1 promoter in vitro and in vivo. ABA is a positive regulator of leaf senescence, and exogenously applied ABA can accelerate Chl degradation. The triple mutant of the ABFs, abf2abf3abf4, as well as two ABA-insensitive mutants, abil-1 and snrk2.2/2.3/2.6, exhibited stay-green phenotypes after ABA treatment, along with decreased induction of NYE1 and NYE2 expression. In contrast, overexpression of ABF4 accelerated Chl degradation upon ABA treatment. Interestingly, ABF2/3/4 could also activate the expression of two Chl catabolic enzyme genes, PAO and NYCl, by directly binding to their promoters. In addition, abf2abf3abf4 exhibited a functional stay-green phenotype, and senescence-associated genes （SAGs）, such as SAG29 （SWEET15）, might be directly regulated by the ABFs. Taken together, our results suggest that ABF2, ABF3, and ABF4 likely act as key regulators in mediating ABA-triggered Chl degradation and leaf senescence in general in Arabidopsis.

关键词： abscisic acid ABF transcription factor chlorophyll degradation leaf senescence Arabidopsisthaliana

The NPR1-WRKY46-WRKY6 signaling cascade mediates probenazole/salicylic acid-elicited leaf senescence in Arabidopsis thaliana

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Journal of Integrative Plant Biology 2021年第5期63卷 924-936页

作者： Dingyu Zhang Zheng Zhu jiong gao Xin Zhou Shuai Zhu Xiaoyan Wang Xiaolei Wang Guodong Ren Benke Kuai State Key Laboratory of Genetic Engineering and Fudan Center for Genetic Diversity and Designing Agriculture Institute of Plant BiologySchool of Life SciencesFudan UniversityShanghai 200438China Institute of Neurology Huashan HospitalFudan UniversityShanghai 200040China Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering Institute of Biodiversity ScienceFudan UniversityShanghai 200438China

Endogenous salicylic acid(SA) regulates leaf senescence, but the underlying mechanism remains largely unexplored. The exogenous application of SA to living plants is not efficient for inducing leaf senescence. By taking advantage of probenazole(PBZ)-inducedbiosynthesisof endogenous SA, we previously established a chemical inducible leaf senescence system that depends on SA biosynthesis and its core signaling receptor NPR1 in Arabidopsis thaliana. Here,using this system, we identified WRKY46 and WRKY6 as key components of the transcriptional machinery downstream of NPR1 signaling. Upon PBZ treatment, the wrky46 mutant exhibited significantly delayed leaf senescence. We demonstrate that NPR1 is essential for PBZ/SA-induced WRKY46 activation, whereas WRKY46 in turn enhances NPR1 expression. WRKY46 interacts with NPR1 in the nucleus, binding to the W-box of the WRKY6 promoter to induce its expression in response to SA signaling. Dysfunction of WRKY6 abolished PBZ-induced leaf senescence, while overexpression of WRKY6 was sufficient to accelerate leaf senescence even under normal growth conditions, suggesting that WRKY6 may serve as an integration node of multiple leaf senescence signaling pathways. Taken together,these findings reveal that the NPR1-WRKY46-WRKY6 signaling cascade plays a critical role in PBZ/SA-mediated leaf senescence in Arabidopsis.

关键词： leaf senescence NPR1 probenazole salicylic acid WRKY46 WRKY6

NON-YELLOWING2 （NYE2）, a Close Paralog of NYE1, Plays a Positive Role in Chlorophyll Degradation in Arabidopsis

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Molecular Plant 2016年第4期9卷 624-627页

作者： Wu, Shouxin Li, Zhongpeng Yang, Lifeng Xie, Zuokun Chen, Junyi Zhang, Wei Liu, Tianqi gao, Shan gao, jiong Zhu, Yihua Xin, Jiwen Ren, Guodong Kuai, Benke Fudan Univ Sch Life Sci Fudan Inst Plant Biol State Key Lab Genet Engn Shanghai 200438 Peoples R China Huazhong Agr Univ Coll Plant Sci & Technol Natl Key Lab Crop Genet Improvement Wuhan 430070 Peoples R China

Dear Editor,Plant senescence is an integral part of plant development, with old organs senescing in association with the emergence and development of nascent ones. Rapid chlorophyll （Chl） degradation, the most prominent event during green organ senescence, is proposed to be a key detoxification process to facilitate massive nutrient remobilization （Lim et al., 2007）.

关键词： senescence integral development

HOPF BIFURCATION OF NONLINEAR ADVERTISING CAPITAL MODEL WITH DISCRETE AND CONTINUOUS TIME DELAYED FEEDBACK

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Annals of Differential Equations 2007年第3期23卷 264-272页

作者： gao Rushan Ruan jiong （Research Center and Laboratory of Mathematics for Nonlinear Science Department and Institute of Mathematics Fudan University Shanghai 200433

In this paper, stability and Hopf bifurcation of a nonlinear advertising ca- pital model with time delayed are studied. By analyzing the change of delay, we obtain several sufficient conditions on stable and unstable properties. When delay passes a critical value, Hopf bifurcation may appear. Furthermore, the direction and stability of bifurcating periodic solutions are investigated by normal form and center manifold theory. Additionally, we also have some discussion about the model with continuous time delay.

关键词： advertising capital model time delay stability Hopf bifurcation normal form center manifold

Malignant transformation of aneurysmal bone cysts： a case report

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Chinese Medical Journal 2009年第1期122卷 110-112页

作者： MEI jiong gao You-shui WANG Shu-qing CAI Xuan-song Department of Orthopedics Tongji Hospital Tongji University Shanghai 200065 China

An aneurysmal bone cyst is a non-neoplastic, .destructive and expansile lesion consisting of a＂blood-filled sponge＂ that is defined to represent a benign reactive vascularity. Szendroi et al suggested that the aneurysmal bone cyst corresponds to a haemodynamic disturbance that is primary or secondary venous malformation of the bones. The association with trauma including fracture has also been noted and 25%-71% of such patients have a history of trauma. Conventionally, malignant transformation of aneurysmal bone cysts is perceived as a rare occurrence except those induced by irradiation. Both malignant fibrous histiocytoma and osteosarcoma have been reported in the malignant transformation cases.

关键词： aneurysmal bone cyst prosthesis implantation malignant fibrous histiocytoma

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高校学报进入Ei收录刊源的几点措施

大连海事大学学报 2006年第2期32卷 143-144页

作者：高炯大连海事大学期刊社辽宁大连116026

对于工科院校而言,论文被Ei收录已成为其学术水平的评价标准之一.探讨学报成为Ei刊源的基本对策,认为应从办刊的基础抓起,开辟高质量稿源,完善Ei对编排格式的要求,从而提高学报Ei的收录率.

关键词：高校学报工程索引检索系统

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井下运输轨道参数检测仪的设计

西安科技大学学报 2011年第2期31卷 198-201页

作者：高建炯中煤张家口煤矿机械有限责任公司张家口075025

针对井下窄轨在受列车运行中产生的纵向力和横向力的作用而发生永久变形的现状,提出一种便携式的井下轨道参数检测分析系统。该系统包括轨道参数采集部分和线路静态检测分析系统两部分。其中检测仪的行进形式采用手推方式。该系统在行... 详细信息

针对井下窄轨在受列车运行中产生的纵向力和横向力的作用而发生永久变形的现状,提出一种便携式的井下轨道参数检测分析系统。该系统包括轨道参数采集部分和线路静态检测分析系统两部分。其中检测仪的行进形式采用手推方式。该系统在行进时能够同时检测铁轨的宽度、水平、里程、三角坑等参数。系统的硬件框架采用基于ARM的嵌入式框架,系统软件的设计采用嵌入式操作系统μC/OS-Ⅱ。该系统不但能显著提高井下轨道的数据检测的工作效率和数据采集精度,而且具有体积小、操作简单的特性。此外,系统的分析部分能对数据进行各种方式的查询、图形对比、图形和报表输出,使得操作者方便迅速地得到轨道的参数,较好地满足了井下轨道维护工作的实际需求。

关键词：铁轨参数数据采集LPC2148 μC/OS-Ⅱ嵌入式系统

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筒体壁厚对传动滚筒应力应变的影响

煤矿机械 2011年第4期32卷 103-105页

作者：高建炯中煤张家口煤矿机械有限责任公司河北张家口075025

传动滚筒作为带式输送机的关键部件,其结构性能的好坏直接影响着带式输送机的可靠性和使用寿命。根据传动滚筒的结构类型、材料属性和工作载荷状况,利用Abaqus进行有限元分析,以1.4 m单端输入传动滚筒为例,进行了筒体壁厚对传动滚筒应... 详细信息

传动滚筒作为带式输送机的关键部件,其结构性能的好坏直接影响着带式输送机的可靠性和使用寿命。根据传动滚筒的结构类型、材料属性和工作载荷状况,利用Abaqus进行有限元分析,以1.4 m单端输入传动滚筒为例,进行了筒体壁厚对传动滚筒应力应变影响的研究。研究表明:当筒体壁厚为22～24 mm比较合理,与实际1.4 m单端传动滚筒筒体壁厚24 mm相吻合,有效地降低了滚筒自身重量,为传动滚筒的设计提供了有利的理论依据。

关键词：筒体壁厚传动滚筒 Abaqus 有限元分析

Synthetic promoters consisting of defined cis-acting elements link multiple signaling pathways to probenazole-inducible system

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第4期16卷 253-263页

作者： Zheng ZHU jiong gao Jin-xiao YANG Xiao-yan WANG Guo-dong REN Yu-long DING Ben-ke KUAI The Southern Modern Forestry Collaborative Innovation Center Nanjing Forestry University College of Biology and the Environment Nanjing Forestry University State Key Laboratory of Genetic Engineering and Institute of Plant Biology School of Life Sciences Fudan University Bamboo Research Institute Nanjing Forestry University

Probenazole （3-allyloxy-l,2-benzisothiazole-1,1-dioxide, PBZ）, the active component of Oryzemate, could induce systemic acquired resistance （SAR） in plants through the induction of salicylic acid （SA） biosynthesis. As a widely used chemical inducer, PBZ is a good prospect for establishing a new chemical-inducible system. We first designed artificially synthetic promoters with tandem copies of a single type of cis-element （SARE, JERE, GCC, GST1, HSRE, and W-box） that could mediate the expression of the tS-glucuronidase （GUS） reporter gene in plants upon PBZ treatment. Then we combined different types of elements in order to improve inducibility in the PBZ-inducible system. On the other hand, we were surprised to find that the cis-elements, which are responsive to jasmonic acid （JA） and ethylene, also responded to PBZ, implying that SA, JA, and ethylene pathways also would play important roles in PBZ＇s action. Further analysis demonstrated that PBZ also induced early events of innate immunity via a signaling pathway in which Ca2＋ influx and mitogen-activated protein kinase （MAPK） activity were involved. We constructed synthesized artificial promoters to establish a PBZ chemical-inducible system, and preliminarily explored SA, JA, ethylene, calcium, and MAPK signaling pathways via PBZ-inducible system, which could provide an insight for in-depth study.

关键词： Probenazole Systemic acquired resistance (SAR) Cis-acting element Chemical-inducible system Synthetic promoter