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不同油菜品种与花椒间作的适应性研究

种业导刊 2024年第2期 42-44页

作者：徐小强高炯赵永强天水市果树研究所甘肃天水741001

为筛选适宜椒园间作的绿肥冬油菜品种,在椒龄8 a的花椒园中进行10个白菜型冬油菜品种的对比试验,分析不同油菜品种盛花期的株高、根长、根直径、根鲜(干)质量、地上部鲜(干)质量、出干率、鲜(干)生物产量等。结果表明,天油1358在盛花期... 详细信息

为筛选适宜椒园间作的绿肥冬油菜品种,在椒龄8 a的花椒园中进行10个白菜型冬油菜品种的对比试验,分析不同油菜品种盛花期的株高、根长、根直径、根鲜(干)质量、地上部鲜(干)质量、出干率、鲜(干)生物产量等。结果表明,天油1358在盛花期时单株生物鲜、干质量分别为195.52、25.20 g,植株含水量87.11%,鲜生物产量88005 kg/hm^(2),干生物产量11340 kg/hm^(2),鲜、干生物产量均排名第1位;其次是陇油7号和陇油16号。综合考虑,天油1358长势强,越冬率高,抗冻性较强,生物产量高,适合在天水黄绵土质地带的花椒园中间作种植。

关键词：油菜绿肥花椒间作品种筛选

REF6 promotes lateral root formation through de-repression of PIN1/3/7 genes

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Journal of Integrative Plant Biology 2019年第4期61卷 383-387页

作者： Xiaolei Wang jiong gao Shan gao Zhongpeng Li Benke Kuai Guodong Ren State Key Laboratory of Genetic Engineering and Fudan Center for Genetic Diversity and Designing Agriculture Institute of Plant Biology School of Life Sciences Fudan University School of Life Sciences Qilu Normal University

The H3K27 methyltransferase CLF inhibits lateral root(LR) formation through depositing the repressive H3K27me3 mark to the chromatin of PIN1, a key polar auxin transporter gene. Here, we show that the H3K27me3 demethylase REF6 promotes lateral root primordium initiation and LR emergence. REF6 directly binds to the chromatin of PIN1/3/7. Dysfunction in REF6 results in increased levels of H3K27me3 on PIN1/3/7 and suppressed expression of PIN genes. Genetic analysis of the clf ref6 double mutant revealed an antagonistic action between CLF and REF6, in terms of LR *** findings indicate that H3K27 methylation and demethylation activities are likely coordinated to ensure proper LR organogenesis.

关键词： REF6 PIN1/3/7 genes^FA

ABF2, ABF3, and ABF4 Promote ABA-Mediated Chlorophyll Degradation and Leaf Senescence by Transcriptional Activation of Chlorophyll Catabolic Genes and Senescence-Associated Genes in Arabidopsis

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Molecular Plant 2016年第9期9卷 1272-1285页

作者： Shan gao jiong gao Xiaoyu Zhu Yi Song Zhongpeng Li Guodong Ren Xin Zhou Benke Kuai State Key Laboratory of Genetic Engineering School of Life Sciences Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering Institute of Biodiversity ScienceFudan University Shanghai 200438 China These authors contributed equally to this article.

Chlorophyll （Chl） degradation is an integral process of leaf senescence, and NYE1/SGR1 has been demonstrated as a key regulator of Chl catabolism in diverse plant species. In this study, using yeast one-hybrid screening, we identified three abscisic acid （ABA）-responsive element （ABRE）-binding transcription factors, ABF2 （AREB1）, ABF3, and ABF4 （AREB2）, as the putative binding proteins of the NYE1 promoter. Through the transactivation analysis, electrophoretic mobility shift assay, and chromatin immunoprecipitation, we demonstrated that ABF2, ABF3, and ABF4 directly bound to and activated the NYE1 promoter in vitro and in vivo. ABA is a positive regulator of leaf senescence, and exogenously applied ABA can accelerate Chl degradation. The triple mutant of the ABFs, abf2abf3abf4, as well as two ABA-insensitive mutants, abil-1 and snrk2.2/2.3/2.6, exhibited stay-green phenotypes after ABA treatment, along with decreased induction of NYE1 and NYE2 expression. In contrast, overexpression of ABF4 accelerated Chl degradation upon ABA treatment. Interestingly, ABF2/3/4 could also activate the expression of two Chl catabolic enzyme genes, PAO and NYCl, by directly binding to their promoters. In addition, abf2abf3abf4 exhibited a functional stay-green phenotype, and senescence-associated genes （SAGs）, such as SAG29 （SWEET15）, might be directly regulated by the ABFs. Taken together, our results suggest that ABF2, ABF3, and ABF4 likely act as key regulators in mediating ABA-triggered Chl degradation and leaf senescence in general in Arabidopsis.

关键词： abscisic acid ABF transcription factor chlorophyll degradation leaf senescence Arabidopsisthaliana

Malignant transformation of aneurysmal bone cysts： a case report

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Chinese Medical Journal 2009年第1期122卷 110-112页

作者： MEI jiong gao You-shui WANG Shu-qing CAI Xuan-song Department of Orthopedics Tongji Hospital Tongji University Shanghai 200065 China

An aneurysmal bone cyst is a non-neoplastic, .destructive and expansile lesion consisting of a＂blood-filled sponge＂ that is defined to represent a benign reactive vascularity. Szendroi et al suggested that the aneurysmal bone cyst corresponds to a haemodynamic disturbance that is primary or secondary venous malformation of the bones. The association with trauma including fracture has also been noted and 25%-71% of such patients have a history of trauma. Conventionally, malignant transformation of aneurysmal bone cysts is perceived as a rare occurrence except those induced by irradiation. Both malignant fibrous histiocytoma and osteosarcoma have been reported in the malignant transformation cases.

关键词： aneurysmal bone cyst prosthesis implantation malignant fibrous histiocytoma

Quick-scanning X-ray absorption fine structure beamline at SSRF

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Nuclear Science and Techniques 2024年第6期35卷 1-13页

作者： Yu Chen Qian gao Zheng Jiang jiong Li Shuo Zhang Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204China National Synchrotron Radiation Laboratory University of Science and Technology of ChinaHefei 230029China

The layout and characteristics of the hard X-ray spectroscopy beamline(BL11B)at the Shanghai synchrotron radiation facility are described ***11B is a bending-magnet beamline dedicated to conventional and millisecond-scale quick-scanning X-ray absorption fine *** is equipped with a cylindrical collimating mirror,a double-crystal monochromator comprising Si(111)and Si(311),a channel-cut quick-scanning Si(111)monochromator,a toroidal focusing mirror,and a high harmonics rejection *** can provide 5-30 keV of X-rays with a photon flux of~5×10^(11)photons/s and an energy resolution of~1.31×10^(-4)at 10 *** performance of the beamline can satisfy the demands of users in the fields of catalysis,materials,and environmental *** paper presents an overview of the beamline design and a detailed description of its performance and capabilities.

关键词： Bending magnet beamline X-ray absorption fine structure Quick-scanning XAFS In situ Synchrotron radiation

The NPR1-WRKY46-WRKY6 signaling cascade mediates probenazole/salicylic acid-elicited leaf senescence in Arabidopsis thaliana

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Journal of Integrative Plant Biology 2021年第5期63卷 924-936页

作者： Dingyu Zhang Zheng Zhu jiong gao Xin Zhou Shuai Zhu Xiaoyan Wang Xiaolei Wang Guodong Ren Benke Kuai State Key Laboratory of Genetic Engineering and Fudan Center for Genetic Diversity and Designing Agriculture Institute of Plant BiologySchool of Life SciencesFudan UniversityShanghai 200438China Institute of Neurology Huashan HospitalFudan UniversityShanghai 200040China Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering Institute of Biodiversity ScienceFudan UniversityShanghai 200438China

Endogenous salicylic acid(SA) regulates leaf senescence, but the underlying mechanism remains largely unexplored. The exogenous application of SA to living plants is not efficient for inducing leaf senescence. By taking advantage of probenazole(PBZ)-inducedbiosynthesisof endogenous SA, we previously established a chemical inducible leaf senescence system that depends on SA biosynthesis and its core signaling receptor NPR1 in Arabidopsis thaliana. Here,using this system, we identified WRKY46 and WRKY6 as key components of the transcriptional machinery downstream of NPR1 signaling. Upon PBZ treatment, the wrky46 mutant exhibited significantly delayed leaf senescence. We demonstrate that NPR1 is essential for PBZ/SA-induced WRKY46 activation, whereas WRKY46 in turn enhances NPR1 expression. WRKY46 interacts with NPR1 in the nucleus, binding to the W-box of the WRKY6 promoter to induce its expression in response to SA signaling. Dysfunction of WRKY6 abolished PBZ-induced leaf senescence, while overexpression of WRKY6 was sufficient to accelerate leaf senescence even under normal growth conditions, suggesting that WRKY6 may serve as an integration node of multiple leaf senescence signaling pathways. Taken together,these findings reveal that the NPR1-WRKY46-WRKY6 signaling cascade plays a critical role in PBZ/SA-mediated leaf senescence in Arabidopsis.

关键词： leaf senescence NPR1 probenazole salicylic acid WRKY46 WRKY6

Synthetic promoters consisting of defined cis-acting elements link multiple signaling pathways to probenazole-inducible system

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第4期16卷 253-263页

作者： Zheng ZHU jiong gao Jin-xiao YANG Xiao-yan WANG Guo-dong REN Yu-long DING Ben-ke KUAI The Southern Modern Forestry Collaborative Innovation Center Nanjing Forestry University College of Biology and the Environment Nanjing Forestry University State Key Laboratory of Genetic Engineering and Institute of Plant Biology School of Life Sciences Fudan University Bamboo Research Institute Nanjing Forestry University

Probenazole （3-allyloxy-l,2-benzisothiazole-1,1-dioxide, PBZ）, the active component of Oryzemate, could induce systemic acquired resistance （SAR） in plants through the induction of salicylic acid （SA） biosynthesis. As a widely used chemical inducer, PBZ is a good prospect for establishing a new chemical-inducible system. We first designed artificially synthetic promoters with tandem copies of a single type of cis-element （SARE, JERE, GCC, GST1, HSRE, and W-box） that could mediate the expression of the tS-glucuronidase （GUS） reporter gene in plants upon PBZ treatment. Then we combined different types of elements in order to improve inducibility in the PBZ-inducible system. On the other hand, we were surprised to find that the cis-elements, which are responsive to jasmonic acid （JA） and ethylene, also responded to PBZ, implying that SA, JA, and ethylene pathways also would play important roles in PBZ＇s action. Further analysis demonstrated that PBZ also induced early events of innate immunity via a signaling pathway in which Ca2＋ influx and mitogen-activated protein kinase （MAPK） activity were involved. We constructed synthesized artificial promoters to establish a PBZ chemical-inducible system, and preliminarily explored SA, JA, ethylene, calcium, and MAPK signaling pathways via PBZ-inducible system, which could provide an insight for in-depth study.

关键词： Probenazole Systemic acquired resistance (SAR) Cis-acting element Chemical-inducible system Synthetic promoter

Landing Gear Shimmy and Directional Stability of Aircraft Undergoing Non-Straight Taxiing

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Chinese Journal of Aeronautics 2001年第2期14卷 73-77页

作者：顾宏斌高泽迥 College of Civil Aviation Nanjing Univ. Aero. Astronautics Nanjing 210016 China

A dynamic model of landing gear struts is introduced in a non-inertial coordinate system. Together with a six degrees of freedom model of aircraft ground handling, the model can be applied to investigate the landing gear shimmy and the directional stability of aircraft undergoing non-straight taxiing. Take the K8 aircraft as an example, motions of the strut under both the straight and non-straight taxiing are simulated as well as the directional adjusting procedure of the airframe. Results of the example are reasonable, indicating that the model may have some value in related research.

关键词： Airframes Degrees of freedom (mechanics) Gears Mathematical models Struts

Integrin β5 subunit regulates hyperglycemia-induced vascular endothelial cell apoptosis through FoxO1-mediated macroautophagy

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Chinese Medical Journal 2024年第5期137卷 565-576页

作者： Xuze Lin Sizhuang Huang Side gao Jinxing Liu jiong Tang Mengyue Yu Department of Cardiology Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100037China Department of Cardiology Fuwai Yunnan Cardiovascular HospitalKunmingYunnan 650000China

Background:Hyperglycemia frequently induces apoptosis in endothelial cells and ultimately contributes to microvascular dysfunction in patients with diabetes mellitus(DM).Previous research reported that the expression of integrins as well as their ligands was elevated in the diseased vessels of DM ***,the association between integrins and hyperglycemia-induced cell death is still *** research was designed to investigate the role played by integrin subunit β5(ITGB5)in hyperglycemia-induced endothelial cell ***:We used leptin receptor knockout(Lepr-KO)(db/db)mice as spontaneous diabetes animal *** deletion of ITGB5 in endothelial cell was achieved by injecting vascular targeted adeno-associated virus via tail ***,we also applied small interfering RNA in vitro to study the mechanism of ITGB5 in regulating high glucose-induced cell ***:ITGB5 and its ligand,fibronectin,were both upregulated after exposure to high glucose in vivo and in ***5 knockdown alleviated hyperglycemia-induced vascular endothelial cell apoptosis and microvascular rarefaction in *** vitro analysis revealed that knockdown of either ITGB5 or fibronectin ameliorated high glucose-induced apoptosis in human umbilical vascular endothelial cells(HUVECs).In addition,knockdown of ITGB5 inhibited fibronectin-induced HUVEC apoptosis,which indicated that the fibronectin-ITGB5 interaction participated in high glucose-induced endothelial cell *** using RNA-sequencing technology and bioinformatic analysis,we identified Forkhead Box Protein O1(FoxO1)as an important downstream target regulated by ***,we demonstrated that the excessive macroautophagy induced by high glucose can contribute to HUVEC apoptosis,which was regulated by the ITGB5-FoxO1 ***:The study revealed that high glucose-induced endothelial cell apoptosis was positively regulated by ITGB5,which suggested that ITGB5 could potentially be used

关键词： Endothelial cell apoptosis Integrin Autophagy Diabetes