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THE DEMAND DISRUPTION MANAGEMENT PROBLEM FOR A SUPPLY CHAIN SYSTEM WITH NONLINEAR DEMAND FUNCTIONS

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Systems Science and Systems Engineering 2003年第1期12卷 82-97页

作者： MinghuiXU XiangtongQI gangyu HanqinZHANG ChengxiuGAO SchoolofMathematicsandStatistics WuhanUniversityWuha430072HubeiP.R.China DepartmentofManagementScienceandInformationSystems TheUniversityofTexasAustinTexas78712U.S.A AcademyofMathematicsandSystemSciences TheChineseAcademyofSciencesBeijing100080P.R.China

This paper addresses the problem of handling the uncertainty of demand in a one-supplier-one-retailer supply chain system. Demand variation often makes the real production different from what is originally planned, causing a deviation cost from the production plan. Assume the market demand is sensitive to the retail price in a nonlinear form, we show how to effectively handle the demand uncertainty in a supply chain, both for the case of centralized-decision-making system and the case of decentralized-decision-making system with perfect coordination.

关键词： Supply chain management disruption management demand uncertainty

Calculation of carbon content of austenite during heat treatment of cast irons

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China Foundry 2010年第1期7卷 30-32页

作者： Gong Wenbang Chen Guodong Xiang gangyu Wuhan University of Science and Engineering Wuhan 430073 Hubei China

The austenitizing temperature controls the carbon content of the austenite which,in turn,influences the structure and properties of cast irons after subsequent cooling to room *** this paper,for a cast iron with known silicon content,a formula of calculating austenite carbon content at a certain austenitizing temperature was *** relationship can be used to more accurately select carbon content of austenite or austenitizing temperature to produce desired properties after subsequent cooling to room temperature.

关键词： cast iron heat treatment austenitizing austenite carbon content of austenite

Expression of cellular FLICE-inhibitory protein and its association with p53 mutation in colon cancer

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World Journal of Gastroenterology 2005年第16期11卷 2482-2485页

作者： Xiao-DongZhou Jie-PingYu Hong-XiaChen Hong-gangyu He-ShengLuo DepartmentofGastroenterology RenminHospitalofWuhanUniversityWuhan430060HubeiProvinceChina DepartmentofGynecologyandObstetrics RenminHospitalofWuhanUniversityWuhan430060HubeiProvinceChina

AIM: To investigate the expression of cellular FLICE (Fas associated death domain-like IL-lbeta-converting enzyme)-inhibitory protein (c-FLIP) and its association with p53 mutation in colon cancer. METHODS: Immunohistochemical staining of c-FLIP and mutant p53 by using specific antibodies was performed by the standard streptavidin-peroxidase technique for 45 colon cancer tissue samples with matched normal tissues. Semi-quantitative reverse transcriptional (RT)-PCR was used to measure c-FLIP mRNA levels, t-test statistical method was used in data analyses. RESULTS: c-FLIP mRNA was expressed in all colon cancer tissues and its level (0.63±0.12) was significantly higher than that in normal tissues (0.38±0.10, P<0.01). Immuno-histochemically, c-FLIP protein was also expressed in all colon cancers (45/45) and 71.1% (32/45) showed an intense immunostaining, in contrast, 93.3% (42/45) of normal colonic mucosa showed positive staining and none of them immunostained intensely. The quantity of c-FLIP protein was significantly higher in cancer tissues than in normal mucosa (7.04±1.20 vs 5.21±0.86, P<0.01). Positive staining of mutant p53 protein was found in 60% (27/45) colon cancers. c-FLIP mRNA level was decreased in p53 positive group compared with p53 negative cancer tissues (0.59±0.13 vs0.69±0.14, P<0.01), but c-FLIP protein had a significantly higher level in p53 positive cancer tissues than in negative ones (7.57±1.30 vs6.25±1.27, P<0.01). CONCLUSION: c-FLIP is specially overexpressed in colon cancers and it might contribute to carcinogenesis of normal colonic mucosa. p53 may exert transcriptional upregulation effects on c-FLI P gene and more potent effects on promoting the degradation of c-FLIP protein.

关键词： Cellular FLICE p53 Colon cancer

Expression and significance of nuclear factor κB p65 in colon tissues of rats with TNBS-induced colitis

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World Journal of Gastroenterology 2005年第12期11卷 1759-1763页

作者： Jun-HuaLi Jie-PingYu Hong-gangyu Xi-MingXu Liang-LiangYu Shi-QuanLiu DepartmentofGastroenterology TheRenminHospitalofWuhanUniversityWuhan430060HubeiProvinceChina

AIM: To investigate the role of NF-κB in the pathogenesis of TNBS-induced colitis in rats. METHODS: Thirty-two healthy adult Sprague-Dawley (SD) rats were randomly divided into four groups of eight each: normal, NS, model Ⅰ, model Ⅱ groups in our study. Rat colitis model was established through 2-,4-,6-trinitrobenzene sulfonic acid (TNBS) enema. At the end of four weeks, the macroscopical and histological changes of the colon were examined and mucosa myeloperoxidase (MPO) activities assayed. NF-κB p65 expression was determined by Western blot assessment in cytoplasmic and nuclear extracts of colon tissue, and the expressions of TNF-α and ICAM-1 protein in colon tissue were examined by immunohistochemistry. The relativities between expression of NF-κB p65 and other parameters were analyzed. RESULTS: TNBS enema resulted in pronounced pathological changes of colonic mucosa in model Ⅱ group (macroscopic and histological injury indices 6.25±1.39 and 6.24±1.04, respectively), which were in accordance with the significantly elevated MPO activity (1.69±0.11). And the nuclear level of NF-κB and expression of TNF-α, ICAM-1 in rats of model II group were higher than that of normal control (9.7±1.96 vs1.7±0.15, 84.09±14.52 vs 16.03±6.21, 77.69±8.09 vs 13.41±4.91 P<0.01), Linear correlation analysis revealed that there were strong correlations between the nuclear level of NF-κB and the tissue positive expression of TNF-α and ICAM-1, MPO activities, macroscopical and histological indices in TNBS-induced colitis, respectively (r= 0.8235, 0.8780, 0.8572, 0.9152, 0.8247; P<0.05). CONCLUSION: NF-κB plays a pivotal role in the pathogenesis of ulcerative colitis, which might account for the up-regulation the expression of TNF-α and ICAM-1.

关键词： NF-κB Ulcerative colitis TNBS

Nuclear factor-kB p65 (RelA) transcription factor is constitutively activated in human colorectal carcinoma tissue

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World Journal of Gastroenterology 2004年第22期10卷 3255-3260页

作者： Liang-LiangYu Hong-gangyu Jie-PingYu He-ShengLuo Xi-MingXu Jun-HuaLi DepartmentofGastroenterology RenminHospitalWuhanUniversityWuhan430060HubeiProvinceChina LaboratoryforExperimentalGastroenterology DepartmentofMedicineISt.JosephHospitalRuhr-UniversityBochumBochumGermany

AIM: Activation of transcription factor nuclear factor-κB(NF-κB) has been shown to play a role in cell proliferation, apoptosis, cytokine production, and oncogenesis. The purpose of this study was to determine whether NF-κB was constitutively activated in human colorectal tumor tissues and, if so, to determine the role of NF-κB in colorectal tumorigenesis, and furthermore, to determine the association of RelA expression with tumor cell apoptosis and the expression of Bcl-2 and Bcl-xL. METHODS: Paraffin sections of normal epithelial, adenomatous and adenocarcinoma tissues were analysed immunohistochemically for expression of RelA, Bcl-2 and Bcl-xL proteins. Electrophoretic mobility shift assay (EMSA) was used to confirm the increased nuclear translocation of RelA in colorectal tumor tissues. The mRNA expressions of Bcl-2 and Bcl-xL were determined by reverse transcription polymerase chain reaction (RT-PCR) analysis. Apoptotic cells were detected triphosphate fluorescence nick end labeling (TUNEL) method. RESULTS: The activity of NF-κB was significantly higher in adenocardnoma tissue in comparison with that in adenomatous and normal epithelial tissues. The apoptotic index (AI) significantly decreased in the transition from adenoma to adenocarcinoma. Meanwhile, the expressions of Bcl-2 and Bcl-xL protein and their mRNAs were significantly higher in adenocarcinoma tissues than that in adenomatous and normal epithelial tissues. CONCLUSION: NF-κB may inhibit apoptosis via enhancing the expression of the apoptosis genes Bcl-2 and Bcl-xL. And the increased expression of RelA/nuclear factor-κB plays an important role in the pathogenesis of colorectal carcinoma.

关键词：原子核基因-κB p65 RelA 转录因子血液活性结直肠癌肿瘤 NF-κB

Analysis of the double-layer a-Si：H emitter with different doping concentrations for α-Si：H/c-Si heterojunction solar cells

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Frontiers in Energy 2017年第1期11卷 92-95页

作者： Haibin HUANG gangyu TIAN Tao WANG Chao GAO Jiren YUAN Zhihao YUE Lang ZHOU Institute of Photovoltaics Nanchang University Nanchang 330031China

Double-layer emitters with different doping concentrations （DLE） have been designed and prepared for amorphous silicon/crystalline silicon （ct-Si：H/c-Si） hetero- junction solar cells. Compared with the traditional single layer emitter, both the experiment and the simulation （AFORS-HET, http：//***/html/releasepaper/2014/04/282/） prove that the double-layer emitter increases the short circuit current of the cells significantly. Based on the quantum efficiency （QE） results and the current-voltage-temperature analysis, the mechanism for the experimental results above has been investigated. The possible reasons for the increased current include the enhancement of the QE in the short wavelength range, the increase of the tunneling probability of the current transport and the decrease of the activation energy of the emitter layers.

关键词： double-layer emitter α-Si:H/c-Si heterojunc-tion solar cell short circuit current quantum efficiency,current-voltage-temperature

F-Actin reassembly during focal adhesion impacts single cell mechanics and nanoscale membrane structure

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Science China Chemistry 2012年第9期55卷 1922-1930页

作者： ZIMMER Christopher C SHI LiFang SHIH YiPing LI JieRen JIN LeeWay LO SuHao LIU gangyu Department of Chemistry University of California Davis CA 95616 USA Department of Biochemistry and Molecular Medicine Center.for Tissue Regeneration and Repair University of California Davis Medical Center Sacramento CA 95817 USA M.I.N.D. Institute and Department of Pathology and Laboratory Medicine ' Alzheimer's Disease Center University of California Davis Medical Center Sacramento CA 95817 USA

Focal adhesions play an important role in cell spreading,migration,and overall mechanical *** relationship of cell structural and mechanical properties was investigated in the context of focal adhesion *** atomic force microscopy(AFM) and laser scanning confocal microscopy(LSCM) was utilized to measure single cell mechanics,in correlation with cellular morphology and membrane structures at a nanometer *** stages of focal adhesion were verified via confocal fluorescent studies,which confirmed three representative F-actin assemblies,actin dot,filaments network,and long and aligned fibrous bundles at ***-deformation profiles of living cells were measured at the single cell level,and displayed as a function of height deformation,relative height deformation and relative volume *** focal adhesion progresses,single cell compression profiles indicate that both membrane and cytoskeleton stiffen,while spreading increases especially from focal complex to focal ***,AFM imaging reveals morphological geometries of spherical cap,spreading with polygon boundaries,and elongated or polarized *** features are dominated by protrusions of 41-207 nm tall,short rods with 1-6 μm in length and 10.2-80.0 nm in height,and long fibrous features of 31-246 nm tall,*** protrusion is attributed to local membrane folding,and the rod and fibrous features are consistent with bilayer decorating over the F-actin *** collectively,the reassembly of F-actin during focal adhesion formation is most likely responsible for the changes in cellular mechanics,spreading morphology,and membrane structural features.

关键词： focal adhesion actin fibers actin assembly fibroblast membrane structure single cell mechanics atomic forcemicroscopy laser scanning confocal microscopy

Styrax japonicus functional genomics:an efficient virus induced gene silencing(VIGS)system

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Horticultural Plant Journal 2024年第1期10卷 252-258页

作者： gangyu Sun Yiqian Ju Cuiping Zhang Lulu Li Xinqiang Jiang Xiaoman Xie Yizeng Lu Kuiling Wang Wei Li College of Landscape Architecture and Forestry Qingdao Agricultural UniversityQingdaoShandong 266109China Shandong Provincial Center of Forest and Grass Germplasm Resources JinanShandong 250102China

VIGS(Virus-induced gene silencing),a method for posttranscriptional gene silencing,is an effective technique for investigating the activities of genes in *** there is no report for available VIGS system in Styrax japonicus,the application of a VIGS approach that results in a gene knockdown to study gene function is *** this study,we compared the characteristics that could affect the viability of VIGS in ***,including the acetosyringone(AS)concentration,the Agrobacterium’s optical density and the inoculation *** stable reference genes of *** were selected to validate the gene’s knockdown by quantitative *** a result,we successfully constructed 2 VIGS systems based on TRV virus:vacuum with AS concentration of 200μmol·L^(-1)and OD600of 0.5,and friction-osmosis with AS concentration of 200μmol·L^(-1)and OD600of 1.0,which silencing efficiency was 83.33%and 74.19%,*** successfully applied VIGS method provides a rapid and effective reverse gene functional analysis approach in *** to identify unknown gene functions.

关键词： Styrax japonicus VIGS Reference gene

Study of a 105 GHz short-pulse dummy load for the electron cyclotron resonance heating system with the quasi-optical method

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Plasma Science and Technology 2022年第10期24卷 139-146页

作者： Shouqi XIONG Donghui XIA Mei HUANG gangyu CHEN Wenting WENG Guoyao FAN Yuan PAN International Joint Research Laboratory of Magnetic Confinement Fusion and Plasma Physics State Key Laboratory of Advanced Electromagnetic Engineering and TechnologySchool of Electrical and Electronic EngineeringHuazhong University of Science and TechnologyWuhan 430074People's Republic of China Southwestern Institute of Physics Chengdu 610041People's Republic of China

The accurate power measurement is important for an ECRH system in *** dummy load is designed and developed for the measurement of the millimeter wave *** work analyzes the dummy load based on the quasi-optical method and the ray tracing *** reflectivity and thermal deposition of the dummy load have been considered to ensure the safety of the entire ***-power tests have been carried out at a 105 GHz/500 kW ECRH *** results of the tests indicate that the designed dummy load is stable and valid.

关键词： dummy load power measurement quasi-optical method ray tracing ECRH