检索结果-南通市图书馆

Development of a tRNA-derived small RNA diagnostic and prognostic signature in liver cancer

在线全文

学校读者我要写书评

暂无评论

Genes & Diseases 2022年第2期9卷 393-400页

作者： Yi Zuo Shaoqiu Chen Lingling Yan Ling Hu Scott Bowler Emory Zitello gang huang Youping Deng Tianyou Hospital Affiliated to Wuhan University of Science and TechnologyWuhan 430064PR China Department of Quantitative Health Sciences John A.Burns School of MedicineUniversity of Hawaii at ManoaHonoluluHI 96813USA Molecular Biosciences and Bioengineering Program College of Tropical Agriculture and Human ResourcesUniversity of Hawaii at ManoaHonoluluHI 96822USA Shanghai Key Laboratory for Molecular Imaging Shanghai University of Medicine and Health SciencesShanghai 201318PR China

Liver cancer presents divergent clinical *** remain opportunities for molecular markers to improve liver cancer diagnosis and prognosis,especially since tRNA-derived small RNAs(tsRNA)have rarely been *** this study,a random forests(RF)diagnostic model was built based upon tsRNA profiling of paired tumor and adjacent normal samples and validated by independent validation(IV).A LASSO model was used to developed a seven-tsRNA-based risk score signature for liver cancer *** performance was evaluated by a receiver operating characteristic curve(ROC curve)and Precision-Recall curve(PR curve).The five-tsRNA-based RF diagnosis model had area under the receiver operating characteristic curve(AUROC)88%and area under the precision–recall curve(AUPR)87%in the discovery cohort and 87%and 86%in IV-AUROC and IV-AUPR,*** seven-tsRNA-based prognostic model predicts the overall survival of liver cancer patients(Hazard Ratio 2.02,95%CI 1.36–3.00,P<0.001),independent of standard clinicopathological prognostic ***,the model successfully categorizes patients into high-low risk *** and prognostic modeling can be reliably utilized in the diagnosis of liver cancer and high-low risk classification of patients based upon tsRNA characterization.

关键词： Diagnosis Liver cancer Prognosis Random forests tRNA-derived small RNAs

Combined detection of plasma GATA5 and SFRP2 methylation is a valid noninvasive biomarker for colorectal cancer and adenomas

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2015年第9期21卷 2629-2637页

作者： Xie Zhang Yu-Fei Song Hong-Na Lu Dan-Ping Wang Xue-Song Zhang Shi-Liang huang Bei-Lei Sun Zhi-gang huang Department of Gastroenterology Ningbo Medical Treatment Center Li Huili Hospital School of Medicine Ningbo University Department of Gastroenterology Zhejiang Provincial People’s Hospital

AIM:To investigate GATA5,SFRP2,and ITGA4 methylation in plasma DNA as noninvasive biomarkers for colorectal cancer(CRC) or ***:There were 57 CRC patients,30 adenomas patients,and 47 control patients enrolled in this ***-specific polymerase chain reaction was used to determine the promoter methylation status of GATA5,SFRP2,and ITGA4 genes in plasma DNA,and their association with clinical outcome in *** predictive ability of GATA5,SFRP2,and ITGA4 methylation,individually or in combination,to detect CRC or adenomas was further ***:Hypermethylated GATA5 was detected in plasma in 61.4%(35/57) of CRC cases,43.33%(13/30) of adenoma cases,and 21.28%(10/47) of control *** hypermethylation of SFRP2 was detected in 54.39%(31/57),40.00%(12/30),and 27.66%(13/47) in plasma samples from CRC,adenomas,and controls,***4 methylation was detected in 36.84%(21/57) of plasma samples of CRC patients and in 30.00%(9/30) of plasma samples from patients with colorectal adenomas,and the specificity of this individual biomarker was 80.85%(9/47).Moreover,GATA5 methylation in the plasma was significantly correlated with larger tumor size(P =0.019),differentiation status(P =0.038),TNM stage(P =0.008),and lymph node metastasis(P =0.008).SFRP2 and ITGA4 methylation in plasma significantly correlated with differentiation status(SFRP2,P =0.012; ITGA4,P =0.007),TNM stage(SFRP2,P =0.034; ITGA4,P =0.021),and lymph node metastasis(SFRP2,P =0.034; ITGA4,P =0.021).From the perspective of predictive power and cost-performance,using GATA5 and SFRP2 together as methylation markers seemed the most favorable predictor for CRC(OR =8.06;95%CI:2.54-25.5; P < 0.01) and adenomas(OR =3.35; 95%CI:1.29-8.71; P =0.012).CONCLUSION:A combination of GATA5 and SFRP2 methylation could be promising as a marker for the detection and diagnosis of CRC and adenomas.

关键词： Colorectal cancer GATA binding protein 5 Secreted

Intestinal tuberculosis with small bowel stricture and hemorrhage as the predominant manifestation: Three case reports

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Surgery 2024年第1期16卷 248-256页

作者： gang huang Kang-Kang Wu Xiao-Na Li Jing-Hua Kuai Ai-Jun Zhang Department of Gastroenterology Qilu Hospital(Qingdao)Cheeloo College of MedicineShandong UniversityQingdao 266000Shandong ProvinceChina

BACKGROUND Intestinal tuberculosis is a chronic disease caused by Mycobacterium tuberculosis that mainly affects the ileum and *** bowel tuberculosis,characterized by predominant involvement of the small intestine,is an extremely rare condition with highly atypical clinical presentations,making diagnosis even more *** SUMMARY We report three cases of small intestinal tuberculosis,two of the patients pre-sented primarily with abdominal pain,and one presented with gastrointestinal *** patients underwent blood tests and imaging *** bowel endoscopy(SBE)revealed that the main lesions in these patients were in-testinal stenosis or gastrointestinal bleeding caused by small intestinal *** patient ultimately underwent surgical *** a complex diagnostic process and comprehensive analysis,all patients were confirmed to have small intestinal tuberculosis and received standard antituberculosis treatment,leading to an improvement in their *** Patients with SBTs present with nonspecific symptoms such as abdominal pain,weight loss,and occasional gastrointestinal *** diagnosis requires a thorough evaluation of clinical symptoms and various tests to avoid misdiagno-sis and complications.

关键词： Intestinal tuberculosis Gastrointestinal hemorrhage Small bowel endoscopy Case report

Nocturnal hypertension and riser pattern are associated with heart failure rehospitalization in patients with heart failure with preserved ejection fraction

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Geriatric Cardiology 2023年第6期20卷 448-458页

作者： gang huang Xiao-Han LIU Yue ZHANG Yu-Xin ZHU Yun HE Tian-Bo WANG Zhen ZHANG Jun-Bo XU Department of Cardiology the Third People’s Hospital of ChengduSichuanChina Cardiovascular Disease Research Institute of Chengdu SichuanChina Affiliated Hospital of Southwest Jiaotong University SichuanChina The Second Affiliated Chengdu Clinical College of Chongqing Medical University SichuanChina

BACKGROUND Nocturnal hypertension is reported as a risk factor for cardiovascular *** study aimed to explore the potential association between nocturnal hypertension and heart failure(HF)rehospitalization in patients with HF with preserved ejection fraction(HFpEF).METHODS A total of 538 patients with HFpEF from May 2018 to December 2021 were consequently recruited in this study and followed up until they were readmitted for HF or the end of this *** regression analysis was used to reveal the potential association between nighttime blood pressure(BP)levels,nocturnal hypertension and nocturnal BP patterns and HF ***-Meier curve was used to assess the cumulative event-free survival rate between *** There were 537 patients with HFpEF were included in the final *** mean age of the study population was 77.14±8.68 years,and 41.2% of patients were *** a median follow-up duration of 10.93(4.19–21.13)months,176 patients(32.7%)with HFpEF were readmitted for *** regression analysis had revealed that nighttime systolic BP level[hazards ratio(HR)=1.018,95%CI:1.008–1.028,P=0.001],nighttime diastolic BP level(HR=1.024,95%CI:1.007–1.042,P=0.007),nocturnal hypertension(HR=1.688,95%CI:1.229–2.317,P=0.001)were associated with HF ***-Meier analysis had demonstrated that patients with nocturnal hypertension had significantly lower event-free survival rate(log-rank P<0.001).Furthermore,patients with a riser pattern had a higher risk of HF rehospitalization(HR=1.828,95%CI:1.055–3.166,P=0.031)and lower eventfree survival rate(log-rank P=0.003)than those with a dipper *** findings were also confirmed in patients with HFpEF and *** Nighttime BP levels,nocturnal hypertension and a riser pattern are independently associated with HF rehospitalization in patients with HFpEF,and prominently in patients with HFpEF and *** controlled nighttime BP levels should be empha

关键词： pattern fraction admitted

Endogenous hydrogen sulfide and ERK1/2-STAT3 signaling pathway may participate in the association between homocysteine and hypertension

在线全文

学校读者我要写书评

暂无评论

Journal of Geriatric Cardiology 2019年第11期16卷 822-834页

作者： Lin SHI Xiao-Yun LIU Zhi-gang huang Zhi-Yi MA Yang XI Lu-Yan WANG Ning-Ling SUN Hypertension Research Laboratory Department of CardiologyPeking University People’s HospitalBeijingChina Emergency Department Peking University Shenzhen HospitalShenzhenChina

Background Homocysteine(Hcy)is a risk factor for hypertension,although the mechanisms are poorly *** We first explored the relationship between Hcy levels and blood pressure(BP)by analyzing the clinical data of primary hypertensive patients admitted to our ***,we explored a rat model to study the effect of Hcy on blood pressure and the role of *** hyperhomocysteinemia(HHcy)rat model was induced to explore the effect of Hcy on blood pressure and the possible *** carried out tissue histology,extraction and examination of RNA and ***,we conducted cell experiments to determine a likely mechanism through renin-angiotensin-aldosterone system(RAAS)and extracellular signal-regulated kinase 1/2(ERK1/2)signaling *** In primary hypertensive inpatients with HHcy,blood pressure was significantly higher as compared with inpatient counterparts lacking *** the rat model,blood pressure of the Wistar rats was significantly increased with increases in serum Hcy levels and decreased after folate *** converting enzyme 1(ACE1)expression in the Wistar Hcy group was enhanced comparing to controls,but was decreased in the Wistar folate *** II receptor type 1(AGTR1)levels in the kidney tissue increased in the Wistar folate *** serum H2S and kidney cystathionineγ-lyase decreased with elevated levels of serum *** vitro,increased concentrations and treatment times for Hcy were associated with increased expression of collagen type 1 and *** dose and time dependent response was also observed for p-STAT3 and p-ERK1/2 *** Endogenous H2S might mediate the process of altered blood pressure in response to changes in serum Hcy levels,in a process that is partly dependent on activated RAAS and ERK1/2-STAT3 signaling pathway.

关键词： Angiotensin converting enzyme 1 Blood pressure ERK1/2-STAT3 signaling pathway Homocysteine Hydrogen sulfide

A solid phase approach to PDMP analogs: A general strategy for combinatorial library

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2013年第9期24卷 845-848页

作者： Chun-Hong Dong Yue-Teng Zhang gang huang Jing-Jing Dong Hong-Xu Liu Xian-Hai Tian Jing Wang Zhi-gang Lv Li-Na Song Wen-Quan Yu College of Chemistry and Molecular Engineering Zhengzhou University Jiaozuo University

The present work reports the first solid phase synthesis of biologically interesting D-threo-l-phenyl-2- decanoylamino-3-morpholino-l-propanol （D-threo-PDMP） derivatives. This synthetic strategy includes facile preparation of versatile azido intermediate （5） in a relatively short sequence and the subsequent derivatization of 5, which led to a series of sulfonamide, urea and heterocycle substituted PDMP analogs （10 and 10＇）. With this method, a 5280-member compound library has been successfully built by IRORI Nanokan system.

关键词： Solid phase synthesis PDMP analogs Combinatorial library

在线全文

学校读者我要写书评

暂无评论

大跨度钢结构雨棚关键节点研究

铁道工程学报 2013年第4期30卷 100-104页

作者：黄刚中铁第四勘察设计院集团有限公司武汉430063

研究目的:大跨度钢结构雨棚关键节点受力复杂、杆件较多,采用常规节点形式往往需要增设插板、外加劲肋等,影响建筑美观;且现行规范对相贯节点设计存在空白之处。本文拟采用铸钢节点形式,并运用有限元分析方法对铸钢节点进行计算分析。... 详细信息

研究目的:大跨度钢结构雨棚关键节点受力复杂、杆件较多,采用常规节点形式往往需要增设插板、外加劲肋等,影响建筑美观;且现行规范对相贯节点设计存在空白之处。本文拟采用铸钢节点形式,并运用有限元分析方法对铸钢节点进行计算分析。研究结论:(1)大跨度钢结构雨棚关键节点采用铸钢节点,可避免多杆焊接时的残余应力、节点设计自由度大、外形设计容易满足建筑造型要求;(2)采用有限元方法可以计算得出铸钢节点的强度破坏、局部稳定破坏和节点的变形,并与设计规范进行校核;(3)通过合理设置节点壁厚、内加劲肋等,可有效降低应力、增强节点刚度、避免局部变形,满足设计要求;(4)本文结论适用于网架、桁架等大跨度钢结构节点设计。

关键词：钢结构雨棚铸钢节点有限元

Could the Recent Taal Volcano Eruption Trigger an El Ni?o and Lead to Eurasian Warming?

在线全文

学校读者我要写书评

暂无评论

Advances in Atmospheric Sciences 2020年第7期37卷 663-670页

作者： Fei LIU Chen XING Jinbao LI Bin WANG Jing CHAI Chaochao GAO gang huang Jian LIU Deliang CHEN School of Atmospheric Sciences and Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies Sun Yat-sen UniversityZhuhai 519082China Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) Zhuhai 519082China Earth System Modeling and Climate Dynamics Research Center Nanjing University of Information Science and TechnologyNanjing 210044China Department of Geography University of Hong KongPokfulamHong Kong Department of Atmospheric Sciences and International Pacific Research Center University of Hawaii at ManoaHonoluluHI 96822USA Department of Environmental Science Zhejiang UniversityHangzhou 310058China State key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China Key Laboratory for Virtual Geographic Environment Ministry of EducationJiangsu Provincial State Key Laboratory Cultivation Base of Geographical Environment EvolutionSchool of Geography ScienceNanjing Normal UniversityNanjing 210023China Department of Earth Sciences University of GothenburgGothenburg 40530Sweden

The Taal Volcano in Luzon is one of the most active and dangerous volcanoes of the Philippines.A recent eruption occurred on 12 January 2020(Fig.1a),and this volcano is still active with the occurrence of volcanic *** eruption has become a deep concern worldwide,not only for its damage on local society,but also for potential hazardous consequences on the Earth’s climate and environment.

关键词： Volcano Philippines worldwide

Statistical analysis of helium bubbles in transmission electron microscopy images based on machine learning method

在线全文

学校读者我要写书评

暂无评论

Nuclear Science and Techniques 2021年第5期32卷 107-117页

作者： Zhong-Hang Wu Ju-Ju Bai Di-Da Zhang gang huang Tian-Bao Zhu Xi-Jiang Chang Ren-Duo Liu Jun Lin Jiu-Ai Sun Department of Scientific Research Shanghai University of Medicine and Health Sciences Affiliated Zhoupu HospitalShanghai 201318China Shanghai Key Laboratory of Molecular Imaging Shanghai University of Medicine and Health SciencesShanghai 201318China Shanghai Institute of Applied Physics Chinese Academy of ScienceShanghai 201800China Shanghai Shanghe Intelligent Technology Co.Ltd. Shanghai 201800China College of Science Donghua UniversityShanghai 201620China School of Medical Imaging Shanghai University of Medicine and Health SciencesShanghai 201318China

Helium bubbles,which are typical radiation microstructures observed in metals or alloys,are usually investigated using transmission electron microscopy(TEM).However,the investigation requires human inputs to locate and mark the bubbles in the acquired TEM images,rendering this task laborious and prone to *** this paper,a machine learning method capable of automatically identifying and analyzing TEM images of helium bubbles is proposed,thereby improving the efficiency and reliability of the *** the proposed technique,helium bubble clusters are first determined via the densitybased spatial clustering of applications with noise algorithm after removing the background and noise *** each helium bubble cluster,the number of helium bubbles is determined based on the cluster size depending on the specific image ***,the helium bubble clusters are analyzed using a Gaussian mixture model,yielding the location and size information on the helium *** contrast to other approaches that require training using numerous annotated images to establish an accurate classifier,the parameters used in the established model are determined using a small number of TEM *** results of the model formulated according to the proposed approach achieved a higher F1 score validated through some helium bubble images manually ***,the established model can identify bubble-like objects that humans cannot facilely *** computationally efficient method achieves object recognition for material structure identification that may be advantageous to scientific work.

关键词： TEM GMM DBSCAN Helium bubble Machine learning