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Creating animal models, why not use the Chinese tree shrew ( Tupaia belangeri chinensis)？

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Zoological Research 2017年第3期38卷 118-126页

作者： Yong-gang yao Key Laboratory of Animal Models and Human Disease Mechanisms Kunming Institute of Zoology Chinese Academy of SciencesKunming Yunnan 650223 China Kunming Primate Research Center of the Chinese Academy of Sciences Kunming Institute of Zoology Chinese Academy of SciencesKunming Yunnan 650223 China

The Chinese tree shrew （Tupaia belangeri chinensis） a squirrel-like and rat-sized mammal, has a wide distribution in Southeast Asia, South and Southwest China and has many unique characteristics that make it suitable for use as an experimental animal. There have been many studies using the tree shrew （Tupaia belangeri） aimed at increasing our understanding of fundamental biological mechanisms and for the modeling of human diseases and therapeutic responses. The recent release of a publicly available annotated genome sequence of the Chinese tree shrew and its genome database （***） has offered a solid base from which it is possible to elucidate the basic biological properties and create animal models using this species. The extensive characterization of key factors and signaling pathways in the immune and nervous systems has shown that tree shrews possess both conserved and unique features relative to primates. Hitherto, the tree shrew has been successfully used to create animal models for myopia, depression, breast cancer, alcohol-induced or non-alcoholic fatty liver diseases, herpes simplex virus type 1 （HSV-1） and hepatitis C virus （HCV） infections, to name a few. The recent successful genetic manipulation of the tree shrew has opened a new avenue for the wider usage of this animal in biomedical research. In this opinion paper, I attempt to summarize the recent research advances that have used the Chinese tree shrew, with a focus on the new knowledge obtained by using the biological properties identified using the tree shrew genome, a proposal for the genome-based approach for creating animal models, and the genetic manipulation of the tree shrew. With more studies using this species and the application of cutting-edge gene editing techniques, the tree shrew will continue to be under the spot light as a viable animal model for investigating the basis of many different human diseases.

关键词： Chinese tree shrew Genome biology Animal model Gene editing Innate immunity

Characterization of long-term ex vivo expansion of tree shrew spermatogonial stem cells

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Zoological Research 2023年第6期44卷 1080-1094页

作者： Cong Li Rui Bi Lin Wang Yu-Hua Ma Yong-gang yao Ping Zheng State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650201China Key Laboratory of Animal Models and Human Disease Mechanisms of Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650201China Kunming College of Life Science University of Chinese Academy of SciencesKunmingYunnan 650204China National Resource Center for Non-Human Primates National Research Facility for Phenotypic&Genetic Analysis of Model Animals(Primate Facility)Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650107China KIZ/CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650204China

Tree shrews(Tupaia belangeri chinensis)share a close relationship to primates and have been widely used in biomedical research.We previously established a spermatogonial stem cell(SSC)-based gene editing platform to generate transgenic tree shrews.However,the influences of long-term expansion on tree shrew SSC spermatogenesis potential remain unclear.Here,we examined the in vivo spermatogenesis potential of tree shrew SSCs cultured across different passages.We found that SSCs lost spermatogenesis ability after long-term expansion(>50 passages),as indicated by the failure to colonize the seminiferous epithelium and generate donor spermatogonia(SPG)-derivedspermatocytesor spermatids marking spermatogenesis.RNA sequencing(RNA-seq)analysis of undifferentiated SPGs across different passages revealed significant gene expression changes after sub-culturing primary SPG lines for more than 40 passages on feeder layers.Specifically,DNA damage response and repair genes(e.g.,MRE11,SMC3,BLM,and GEN1)were down-regulated,whereas genes associated with mitochondrial function(e.g.,NDUFA9,NDUFA8,NDUFA13,and NDUFB8)were up-regulated after expansion.The DNA damage accumulation and mitochondrial dysfunction were experimentally validated in high-passage cells.Supplementation with nicotinamide adenine dinucleotide(NAD+)precursor nicotinamide riboside(NR)exhibited beneficial effects by reducing DNA damage accumulation and mitochondrial dysfunction in SPG elicited by long-term culture.Our research presents a comprehensive analysis of the genetic and physiological attributes critical for the sustained expansion of undifferentiated SSCs in tree shrews and proposes an effective strategy for extended in vitro maintenance.

关键词： Tree shrews Spermatogonial stem cells Nicotinamide riboside DNA damage Mitochondrial dysfunction

Electronic and Optical Properties of Rocksalt CdO: A first-Principles Density-Functional Theory Study

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Modeling and Numerical Simulation of Material Science 2013年第1期3卷 16-19页

作者： gang yao Xinyou An Hongwen Lei Yajun Fu Weidong Wu Science and Technology on Plasma physics Laboratory Research Center of Laser Fusion CAEP P.O.Box 919-983 Mianyang 621900 P.R. China Science and Technology on Plasma physics Laboratory Research Center of Laser Fusion CAEP P.O.Box 919-983 Mianyang 621900 P.R. China State Key Laboratory Cultivation Base for Nonmetal Composites and Functional Materials School of Materials Science and Engineering Southwest University of Science and Technology Mianyang 621010 P.R. China Science and Technology on Plasma physics Laboratory Research Center of Laser Fusion CAEP P.O.Box 919-983 Mianyang 621900 P.R. China State Key Laboratory Cultivation Base for Nonmetal Composites and Functional Materials School of Materials Science and Engineering Southwest University of Science and Technology Mianyang 621010 P.R. China

The structural, electronic and optical properties of rocksalt CdO have been studied using the plane-wave-based pseudo-potential density functional theory within generalized gradient approximation. The calculated lattice parameters are in agreement with previous experimental work. The band structure, density of states, and Mulliken charge population are obtained, which indicates that rocksalt CdO having the properties of a halfmetal due to an indirect band gap of -0.51eV. The mechanical properties show that rocksalt CdO is mechanically stable, isotropic and malleable. Significantly, we propose a correct value for ε1(0) of about 4.75, which offers theoretical data for the design and application for rocksalt CdO in optoelectronic materials.

关键词： Density-Functional Theory Electronic Structure Optical Properties Rocksalt CdO

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降解性塑料薄膜材料的研究现状与应用潜力

天津造纸 2024年第1期46卷 22-26,38页

作者：姚刚李宝庆梁欣姜俊志阎瑞香深圳顺丰泰森控股(集团)有限公司深圳518000 天津科技大学轻工科学与工程学院天津300457

用传统塑料薄膜对纸张进行覆膜处理已不能满足市场需求,可降解塑料薄膜材料的应用与推广,为提高传统覆膜纸的生态友好性提供了新思路。本文阐述了可降解塑料薄膜的分类、降解机理和制备工艺,概述了降解性塑料薄膜的发展现状与应用潜力,... 详细信息

用传统塑料薄膜对纸张进行覆膜处理已不能满足市场需求,可降解塑料薄膜材料的应用与推广,为提高传统覆膜纸的生态友好性提供了新思路。本文阐述了可降解塑料薄膜的分类、降解机理和制备工艺,概述了降解性塑料薄膜的发展现状与应用潜力,以期为高效率、多领域利用可降解塑料薄膜提供理论支持。今后对降解性塑料薄膜材料的开发应用,除了成本外,应更多关注于材料的生态友好性,同时积极扩展其应用领域。

关键词：降解性塑料薄膜降解机理制备工艺

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航空背景下“工程制图”课程思政课堂构建

教育教学论坛 2024年第11期 177-180页

作者：张冉阳赵刚要郭正华南昌航空大学航空制造工程学院江西南昌330063

航空专业背景下的“工程制图”,不仅具有一般工科工程图绘制的基本特征,而且涉及飞行器常用零部件特有的绘制方法、飞行器零件图画法规定及其装配图画法规定等。针对具有航空特色专业的“工程制图”课程教学,引领航空文化融入“工程制... 详细信息

航空专业背景下的“工程制图”,不仅具有一般工科工程图绘制的基本特征,而且涉及飞行器常用零部件特有的绘制方法、飞行器零件图画法规定及其装配图画法规定等。针对具有航空特色专业的“工程制图”课程教学,引领航空文化融入“工程制图”教学全过程,深挖“工程制图”教学内容中蕴含的思政元素,构建“工程制图”思想政治理论课堂,把思想政治教育有机融入“工程制图”教学全过程;在培养学生形体想象、创造和工程设计能力的同时,致力于培养具有优秀团队合作精神、工匠精神和忠诚奉献、航空报国精神的高素质人才。

关键词：航空文化工程制图课程思政思政元素

Recent advances of ferro-/piezoelectric polarization effect for dendrite-free metal anodes

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稀有金属:英文版 2023年第8期42卷 2516-2544页

作者： Hai-Xia Zhang Peng-Fei Wang Chuan-gang yao Shi-Peng Chen Ke-Di Cai Fa-Nian Shi School of Materials Science and Engineering Shenyang University of TechnologyShenyang 110870China School of Environmental and Chemical Engineering Shenyang University of TechnologyShenyang 110870China College of Chemistry and Materials Engineering Bohai UniversityJinzhou 121013China

Metal anodes based on plating/stripping electrochemistry,for instance,common alkaline metal lithium(Li),sodium(Na),potassium(K),polyvalent metal magnesium(Mg),aluminum(Al),calcium(Ca)and zinc(Zn)are imminently evoked and increasingly researched for future generation high-energy-density rechargeable batteries due to their large theoretical capacity,low electrochemical potential,and superior electronic conductivity in recent years.However,the uncontrolled dendrite formation issue induces low Coulombic efficiency,short lifespan,and hazardous security risks,hindering the actual applications of metal batteries.Among various solutions,the utilization of ferro-/piezoelectric materials for metal anodes displays active effects on decreasing local current density,suppressing dendrite growth,and tolerating volume expansion benefits from the unique ferro-/piezoelectric polarization effect.This review presents the research progress of ferro-/piezoelectric polarization effect for regulating the dendritic growth of metal anodes for the first time.First,the current challenges and strategies of metal anodes are proposed.Then,ferro-/piezoelectric materials and their working principle are discussed.Finally,the recent research progress of ferroelectric and piezoelectric materials on dynamic regulation of dendrite growth is summarized,and the future perspectives are prospected.We hope this review could draw more attention in designing metal anodes with self-polarization materials and promoting their practical applications.

关键词： Metal anode Ferro-/piezoelectric material Polarization effect Dendrite-free Lithium Perovskite

模型和数据驱动结合的暖通空调需求响应成本分析

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电力需求侧管理 2024年第2期26卷 27-33页

作者：赵本源赵建立张沛超姚刚杜江贵州电网有限责任公司电力调度控制中心贵阳550002 国网上海市电力公司上海200030 电力传输与功率变换控制教育部重点实验室(上海交通大学) 上海200240

为实现楼宇暖通空调与电网友好互动,提出了模型与数据驱动相结合的需求响应成本分析。首先,利用人工神经网络的拟合能力,从物理模型中自动学习并构建动态模型。然后,考虑温度变化对楼宇人员工作效率的影响,建立暖通空调设定温度的优化... 详细信息

为实现楼宇暖通空调与电网友好互动,提出了模型与数据驱动相结合的需求响应成本分析。首先,利用人工神经网络的拟合能力,从物理模型中自动学习并构建动态模型。然后,考虑温度变化对楼宇人员工作效率的影响,建立暖通空调设定温度的优化模型并基于粒子群优化算法进行求解。最后,将用户因偏离最优设定温度而产生的额外成本定义为响应成本,据此提出暖通空调的需求响应成本分析方法。仿真结果表明,通过设定暖通空调的最优温度,可显著降低用户的总成本;响应成本曲线可反映用户的工作效率损失因素,从而帮助用户在需求响应中理性确定投标价格。

关键词：暖通空调需求响应成本人工神经网络工作效率

Tau Accumulation in the Spinal Cord Contributes to Chronic Inflammatory Pain by Upregulation of IL-1βand BDNF

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Neuroscience Bulletin 2024年第4期40卷 466-482页

作者： Shuxia Zhang Yeru Chen Yongjie Wang Hongwei Wang Dandan yao gang Chen Department of Anesthesiology School of MedicineSir Run Run Shaw HospitalZhejiang UniversityHangzhou310016China Key Laboratory of Elemene Anti-Cancer Medicine of Zhejiang Province and Holistic Integrative Pharmacy Institutes Hangzhou Normal UniversityHangzhou311121China Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang Province Holistic Integrative Pharmacy InstitutesHangzhou Normal UniversityHangzhou311121China

Microtubule-associated protein Tau is responsible for the stabilization of neuronal microtubules under normal physiological conditions.Much attention has been focused on Tau’s contribution to cognition,but little research has explored its role in emotions such as pain,anxiety,and depression.In the current study,we found a significant increase in the levels of p-Tau(Thr231),total Tau,IL-1β,and brain-derived neurotrophic factor(BDNF)on day 7 after complete Freund's adjuvant(CFA)injection;they were present in the vast majority of neurons in the spinal dorsal horn.Microinjection of Mapt-shRNA recombinant adeno-associated virus into the spinal dorsal cord alleviated CFA-induced inflammatory pain and inhibited CFA-induced IL-1βand BDNF upregulation.Importantly,Tau overexpression was sufficient to induce hyperalgesia by increasing the expression of IL-1βand BDNF.Furthermore,the activation of glycogen synthase kinase 3 beta partly contributed to Tau accumulation.These findings suggest that Tau in the dorsal horn could be a promising target for chronic inflammatory pain therapy.

关键词： Tau Inflammatory pain IL-1β BDNF

屡配不孕母牛FOXP3、FSHR、FMR1基因多态性与生殖激素相关性分析

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畜牧兽医学报 2024年第6期55卷 2727-2740页

作者：王选艺孙亚伟龙雨薇王俪颖周渝新李娜马雪连赵红琼姚刚新疆农业大学动物医学学院乌鲁木齐830052

旨在研究叉头框蛋白P3基因(forkhead box protein P3,FOXP3)、促卵泡激素受体基因(follicle stimulating hormone receptor,FSHR)、智力低下1基因(fragile X mental retardation 1,FMR1)的多态性,并探讨了基因多态性对母牛屡配不孕以及母牛激素水平的影响。通过PCR方法扩增基因突变位点的序列,对PCR结果进行酶切鉴定和测序峰图分析,统计三种基因的基因型。分析三种基因突变的组间差异。使用ELISA法检测两组雌激素(estrogen, E)、促卵泡激素(follicle-stimulating hormone, FSH)、催乳素(prolactin, PRL)、睾酮素(testosterone, T)、促黄体生成素(luteinizing hormone, LH)激素浓度。并将基因多态性与激素水平进行关联分析。FOXP3基因检测出3种基因型:AA、AG和GG型;FSHR基因检测出3种基因型:AA、AC和CC型;FMR1基因5′UTR端302 bp处检测到T碱基插入。卡方适应性检验结果表明,FOXP3基因g.92,377,635 A>G位点、FSHR基因g.31,450,279 A>C位点均低于哈代-温伯格平衡状态(PG位点G等位基因与母牛患屡配不孕疾病呈显著正相关(PC位点C等位基因与母牛屡配不孕具有显著正相关性(P<0.05,r=0.14)。FOXP3以及FSHR基因的多态性位点可能作为母牛屡配不孕的标志性识别位点,可用于筛选屡配不孕母牛的潜在候选遗传标记,这需要进一步的证明。FOXP3基因G等位基因与E浓度呈弱正相关(P<0.05,r=0.16),FMR1基因T碱基的插入与E浓度呈弱正相关(P<0.05,r=0.187)。然而FOXP3、FSHR基因的变异如何调控母牛繁殖能力还需进一步研究证实。

关键词：牛屡配不孕基因多态性激素关联分析

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