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Dietary supplementation of ferrous glycinate improves intestinal barrier function by modulating microbiota composition in Cherry Valley ducks

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Animal Nutrition 2022年第4期11卷 264-275页

作者： Haihua Yu Yueqin Xie Bing Wu Hua Zhao Xiaoling Chen gang Tian Guangmang Liu Jingyi Cai Gang Jia Institute of Animal Nutrition Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of EducationSichuan Agricultural UniversityChengduSichuan611130China Chelota Group Guanghan618300China

Ferrous glycinate(Fe-Gly)has been increasingly used as iron fortification in the diets of weaned piglets and broilers,but the effect of Fe-Gly on intestinal barrier function in meat ducks has not been well defined.This study therefore investigated the effect of Fe-Gly on apparent nutrient utilization,hematological indices,intestinal morphological parameters,intestinal barrier function and microbial composition in meat ducks.A total of 672 one-day-old Cherry Valley ducks were randomly divided into 6treatments(8 replicates for each treatment and 14 ducks for each replicate)and fed diets with 0(control),30,60,90 and 120 mg/kg Fe-Gly or 120 mg/kg Fe SO4for 35 d.The results showed that diets supplemented with Fe-Gly significantly increased average daily gain(ADG),average daily feed intake(ADFI),hematocrit(HCT),mean cell volume(MCV),the apparent utilization of dry matter(DM)and metabolizable energy(ME),villus height(VH)and villus height-to-crypt depth ratio(V:C)(P<0.05).FeGly also significantly up-regulated barrier-related genes including zonula occludens-1(ZO-1),zonula occludens-2(ZO-2),mucin 2(MUC2)and lysozyme(LYZ)(P<0.05),and down-regulated the m RNA expression of claudin-2(CLDN2)and occludin(OCLN)in the jejunum(P<0.05).The 16S r RNA sequence analysis indicated that the diet with Fe-Gly had a higher relative abundance of Intestinimonas and Romboutsia(P<0.05),which have an ability to produce short chain fatty acids(SCFAs),especially butyric acid.It also decreased the relative abundance of pathobiont,including Megamonas,Eubacterium_coprostanoligenes_group and Plebeius(P<0.05).Additionally,diets supplemented with 120 mg/kg Fe-Gly significantly increased the apparent utilization of DM and ME(P<0.05)and decreased the relative abundance of Megamonas_unclassified and Bacteroides_unclassified compared with those fed120 mg/kg Fe SO4(P<0.05).These results revealed that diets supplemented with Fe-Gly exerted a potent beneficial effect on physical,chemical,immune and microbial barriers,thereby improving the integrity of the in

关键词： Ferrous glycinate Bacterial microbiome Barrier function Cherry Valley duck

Spermine protects intestinal barrier integrity through ras-related C3 botulinum toxin substrate 1/phospholipase C-γ1 signaling pathway in piglets

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Animal Nutrition 2022年第1期8卷 135-143页

作者： Guangmang Liu Xiaomei Xu Caimei Wu Gang Jia Hua Zhao Xiaoling Chen gang Tian Jingyi Cai Jing Wang Institute of Animal Nutrition Sichuan Agricultural UniversityChengdu611130China Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Chengdu611130China Maize Research Institute Sichuan Agricultural UniversityChengdu611130China

Weaning stress can cause tight junctions damage and intestinal permeability enhancement,which leads to intestinal imbalance and growth retardation,thereby causing damage to piglet growth and development.Spermine can reduce stress.However,the mechanism of spermine modulating the intestinal integrity in pigs remains largely unknown.This study aims to examine whether spermine protects the intestinal barrier integrity of piglets through ras-related C3 botulinum toxin substrate 1(Rac1)/phospholipase C-g1(PLC-γ1)signaling pathway.In vivo,80 piglets were categorised into 4 control groups and 4 spermine groups(10 piglets per group).The piglets were fed with normal saline or spermine at 0.4 mmol/kg BW for 7 h and 3,6 and 9 d.In vitro,we investigated whether spermine protects the intestinal barrier after a tumor necrosis factor a(TNF-a)challenge through Rac1/PLC-γ1 signaling pathway.The in vivo study found that spermine supplementation increased tight junction protein mRNA levels and Rac1/PLC-γ1 signaling pathway gene expression in the jejunum of piglets.The serum D-lactate content was significantly decreased after spermine supplementation(P<0.05).The in vitro study found that 0.1 mmol/L spermine increased the levels of tight junction protein expression,Rac1/PLC-γ1 signaling pathway and transepithelial electrical resistance,and decreased paracellular permeability(P<0.05).Further experiments demonstrated that spermine supplementation enhanced the levels of tight junction protein expression,Rac1/PLC-γ1 signaling pathway and transepithelial electrical resistance,and decreased paracellular permeability compared with the NSC-23766 and U73122 treatment with spermine after TNF-a challenge(P<0.05).Collectively,spermine protects intestinal barrier integrity through Rac1/PLC-γ1 signaling pathway in piglets.

关键词： Spermine Intestinal barrier Rac1/PLC-γ1 signaling pathway Intestinal integrity

Selenogenome and AMPK signal insight into the protective effect of dietary selenium on chronic heat stress-induced hepatic metabolic disorder in growing pigs

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Journal of Animal Science and Biotechnology 2022年第1期13卷 152-165页

作者： Yan Liu jiayong Tang Ying He Gang Jia Guangmang Liu gang Tian Xiaoling Chen Jingyi Cai Bo Kang Hua Zhao Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Key Laboratory for Animal Disease-Resistance Nutrition Ministry of EducationHuimin RoadWenjiang DistrictChengdu 611130SichuanChina College of Animal Science and Technology Sichuan Agricultural UniversityChengdu 611130SichuanChina

Background:Chronic heat stress(CHS)disrupts hepatic metabolic homeostasis and jeopardizes product quality of pigs.Selenium(Se)may regulate the metabolic state through affect selenoprotein.Thus,we investigate the protective effect of dietary hydroxy-4-methylselenobutanoic acid(HMSeBA)on CHS induced hepatic metabolic disorder in growing pigs,and the corresponding response of selenoprotein.Methods:Forty crossbreed growing pigs were randomly assigned to five groups:control group raised in the thermoneutral environment(22±2℃)with basal diet;four CHS groups raised in hyperthermal condition(33±2℃)with basal diet and supplied with 0.0,0.2,0.4,and 0.6 mg Se/kg HMSeBA,respectively.The trial lasted 28 d.The serum biochemical,hepatic metabolism related enzyme,protein and gene expression and 25 selenoproteins in liver tissue were determined by real-time PCR,ELISA and western blot.Results:CHS significantly increased the rectal temperature,respiration rate,serum aspartate aminotransferase(AST)and low-density lipoprotein cholesterol(LDL-C)of pigs,up-regulated hepatic heat shock protein 70(HSP70)and induced lower liver weight,glycogen content,hepatic glucokinase and glutathione peroxidase(GSH-Px).The CHSinduced liver metabolic disorder was associated with the aberrant expression of 6 metabolism-related gene and 11 selenoprotein encoding genes,and decreased the protein abundance of GCK,GPX4 and SELENOS.HMSeBA improved anti-oxidative capacity of liver.0.4 or 0.6 mg Se/kg HMSeBA supplementation recovered the liver weight,glycogen content and rescue of mRNA abundance of genes related to metabolism and protein levels of GCK.HMSeBA supplementation changed expressions of 15 selenoprotein encoding genes,and enhanced protein expression of GPX1,GPX4 and SELENOS in the liver affected by CHS.CHS alone showed no impact while HMSeBA supplementation increased protein levels of p-AMPKαin the liver.Conclusions:In summary,HMSeBA supplementation beyond nutrient requirement mitigates CHS-induced hepatic metabolic disorder,recovered the liver glycoge

关键词： Chronic heat stress Hepatic metabolism HMSeBA Pigs Selenoprotein

Optimization of closing law of turbine guide vanes based on improved artificial ecosystem algorithm

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水动力学研究与进展B辑:英文版 2023年第3期35卷 582-593页

作者： Li-ying Wang jia-jie Zhang Hong-gang Fan College of Water Conservancy and Hydropower Hebei University of EngineeringHandan 056038China Hebei Key Laboratory of Smart Water Conservancy Handan 056038China State Key Laboratory of Hydroscience and Engineering Department of Energy and Power EngineeringTsinghua UniversityBeijing 100084China

Optimization of the closing law of the guide vane is the most economical and efficient way to reduce the risk incurred by pressure and speed excursions,thus guaranteeing the security of the hydro-turbine and the whole hydraulic network.In order to optimize the closing law of the guide vane of hydraulic turbine,an improved artificial ecosystem optimization algorithm was proposed(IAEO).The reverse learning was used to initialize the population,multi-strategy bound handing schemes was used to improve the algorithm convergence speed.Twenty-three mathematical benchmark functions were used to test the IAEO.Results showed an improvement in the IAEO algorithm convergence speed and a stronger exploration than other algorithms.IAEO algorithm was used to optimize the closing law of the guide vane of hydraulic turbine based on the hydraulic transient calculation.The results showed that the maximum pressure in the spiral casing inlet,the minimum pressure in the draft tube inlet and the maximum speed all meet the design requirements by use of the closing law of the guide vane optimized by IAEO.Compared with other algorithms such as particle swarm optimization(PSO),artificial ecosystem-based optimization(AEO)and grey wolf optimizer(GWO),the closing law of the guide vane optimized by IAEO algorithm was proved to be of great advantages in distribution of safety margin of each optimization goal.

关键词： Hydraulic transient process the closing law of guide vane improved artificial ecosystem-based optimization(IAEO) optimization calculation

2-divisibility of Some Odd Hole Free Graphs

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Acta Mathematicae Applicatae Sinica 2022年第3期38卷 710-718页

作者： Wei DONG jia-lei SONG Bao-gang XU School of Information and Engineering Nanjing Xiaozhuang UniversityNanjing 211171China Institute of Mathematics School of Mathematical SciencesNanjing Normal UniversityNanjing 210023China

Let G be a graph.We say that G is 2-divisible if for each induced subgraph H of G,either V(H)is a stable set,or V(H)can be partitioned into two sets A and B such thatω(H[A])<ω(H)andω(H[B])<ω(H).A hole is an induced cycle of length at least 4,a bull is a graph consisting of a triangle with two disjoint pendant edges,a diamond is the graph obtained from K4 by removing an edge,a dart denotes the graph obtained from a diamond by adding a pendant edge to one vertex of degree 3,and a racket denotes the graph obtained from a diamond by adding a pendant edge to one vertex of degree 2.In this paper,we prove that every{odd hole,H}-free graph is 2-divisible,where H is a dart,or a racket,or a bull.As corollaries,X(G)≤min{2ω^(G)-1,(ω^(G)/2+1)}if G is{odd hole,dart}-free,or{odd hole,racket}-free,or{odd hole,bull}-free.

关键词： hole odd hole 2-divisibility

Selenoproteins synergistically protect porcine skeletal muscle from oxidative damage via relieving mitochondrial dysfunction and endoplasmic reticulum stress

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畜牧与生物技术杂志:英文版 2023年第5期14卷 2180-2196页

作者： Jinzhong Jing Ying He Yan Liu jiayong Tang Longqiong Wang Gang Jia Guangmang Liu Xiaoling Chen gang Tian Jingyi Cai Lianqiang Che Bo Kang Hua Zhao Key Laboratory for Animal Disease‑Resistance Nutrition of Ministry of Education of China Ministry of Agriculture and Rural Affairsof Sichuan ProvinceAnimal Nutrition InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina College of Animal Science and Technology Sichuan Agricultural UniversityChengdu 611130SichuanChina

Background The skeletal muscle of pigs is vulnerable to oxidative damage,resulting in growth retardation.Selenoproteins are important components of antioxidant systems for animals,which are generally regulated by dietary selenium(Se)level.Here,we developed the dietary oxidative stress(DOS)-inducing pig model to investigate the protective effects of selenoproteins on DOS-induced skeletal muscle growth retardation.Results Dietary oxidative stress caused porcine skeletal muscle oxidative damage and growth retardation,which is accompanied by mitochondrial dysfunction,endoplasmic reticulum(ER)stress,and protein and lipid metabolism disorders.Supplementation with Se(0.3,0.6 or 0.9 mg Se/kg)in form of hydroxy selenomethionine(OH-SeMet)linearly increased muscular Se deposition and exhibited protective effects via regulating the expression of selenotranscriptome and key selenoproteins,which was mainly reflected in lower ROS levels and higher antioxidant capacity in skeletal muscle,and the mitigation of mitochondrial dysfunction and ER stress.What’s more,selenoproteins inhibited DOS induced protein and lipid degradation and improved protein and lipid biosynthesis via regulating AKT/mTOR/S6K1 and AMPK/SREBP-1 signalling pathways in skeletal muscle.However,several parameters such as the activity of GSH-Px and T-SOD,the protein abundance of JNK2,CLPP,SELENOS and SELENOF did not show dose-dependent changes.Notably,several key selenoproteins such as MSRB1,SELENOW,SELENOM,SELENON and SELENOS play the unique roles during this protection.Conclusions Increased expression of selenoproteins by dietary OH-SeMet could synergistically alleviate mitochondrial dysfunction and ER stress,recover protein and lipid biosynthesis,thus alleviate skeletal muscle growth retardation.Our study provides preventive measure for OS-dependent skeletal muscle retardation in livestock husbandry.

关键词： Dietary oxidative stress Endoplasmic reticulum stress Growth retardation Mitochondrial dysfunction Selenoproteins Skeletal muscle

Periodontal Ligament Stem Cells Reverse the High Glucose Level-Induced Inflammatory State of Macrophages

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Biomedical and Environmental Sciences 2022年第7期35卷 674-679页

作者： LIU Yun Xia DING Xiao Ling TANG Shuai CUI Shu Yue LIU jia jia DING gang School of Stomatology Weifang Medical UniversityWeifang 261053ShandongChina Clinical Competency Training Center Weifang Medical UniversityWeifang 261053ShandongChina

Periodontitis is a chronic infectious disease characterized by plaque biofilm as its main pathogenic factor;its clinical manifestations include gingival inflammation,alveolar bone resorption,attachment loss,and periodontal pocket formation,all of which eventually lead to tooth loss and seriously affect the quality of life of patients[1].This illness has a close relationship with blood sugar levels in patients with diabetes and is one of the most important complications of diabetes[2].Macrophages are an important part of host immune responses and exhibit powerful functions of identifying,phagocytizing,and removing bacteria;macrophage polarization is involved in the pathogenesis of periodontitis[3].

关键词： inflammation removing eventually

Forecast of natural gas supply and demand in China under the background of “Dual Carbon Targets”

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Petroleum Exploration and Development 2023年第2期50卷 492-504页

作者： jia Ailin CHENG gang CHEN Weiyan LI Yilong PetroChina Research Institute of Petroleum Exploration&Development Beijing 100083China

As a kind of clean energy which creates little carbon dioxide, natural gas will play a key role in the process of achieving “Peak Carbon Dioxide Emission” and “Carbon Neutrality”. The Long-range Energy Alternatives Planning System(LEAP) model was improved by using new parameters including comprehensive energy efficiency and terminal effective energy consumption. The Back Propagation(BP) Neural Network–LEAP model was proposed to predict key data such as total primary energy consumption, energy mix, carbon emissions from energy consumption, and natural gas consumption in China. Moreover, natural gas production in China was forecasted by the production composition method. Finally, based on the forecast results of natural gas supply and demand, suggestions were put forward on the development of China’s natural gas industry under the background of “Dual Carbon Targets”. The research results indicate that under the background of carbon peak and carbon neutrality, China’s primary energy consumption will peak(59.4×10^(8)tce) around 2035, carbon emissions from energy consumption will peak(103.4×10^(8)t) by 2025, and natural gas consumption will peak(6100×10^(8)m^(3)) around 2040, of which the largest increase will be contributed by the power sector and industrial sector. China’s peak natural gas production is about(2800–3400)×10^(8)m^(3), including(2100–2300)×10^(8)m^(3)conventional gas(including tight gas),(600–1050)×10^(8)m^(3)shale gas, and(150–220)×10^(8)m^(3)coalbed methane. Under the background of carbon peak and carbon neutrality, the natural gas consumption and production of China will further increase, showing a great potential of the natural gas industry.

关键词： carbon peak and carbon neutrality energy mix carbon emissions natural gas consumption natural gas produc-tion new energy system terminal consumption scale production,supply,storage and marketing

Novel hollow microsphere with porous carbon shell embedded with Cu/Co bimetal nanoparticles:Facile large-scale preparation and catalytic hydrogenation performance

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Journal of Materials Science & Technology 2022年第27期122卷 44-53页

作者： Gaiping Du Ran Liu Qianqian jia gang Han Zhenguo An Jingjie Zhang Key Laboratory of Photochemical Conversion and Optoelectronic Materials Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China

Non-noble bimetals have attracted extensive attention for their natural aboundance and low cost,but it remains a big challenge to design and synthesize novel supported non-noble bimetal nanocatalyst in a controllable and high-efficient manner.Herein,a novel hollow spherical supported non-noble bimetal nanocatalyst with porous carbon shell as the continuous matrix and Cu/Co bimetal nanoparticles as the dispersion phase is successfully fabricated by a convenient strategy involving spray drying and subsequent heat treatment.The morphology and microstructure depend catalyst activity of the hollow spherical supported catalyst has been studied systematically.It is found that the heating temperature plays a critical role in determining the microstructure and catalytic performance of the products.With an optimal heating temperature of 600°C,the corresponding product exhibits the highest normalized reaction rate constant(k_(n))of 25.4 s^(-1)g^(-1)for catalytic reduction of 4-notrophenol,which can be attributed to the suitable synergism of the well-defined bimetal structure,combined effect of the two metallic phases and the metal-support interaction.This work provides an additional strategy for the simultaneous formation of both the support and the active loading phase of supported non-noble bimetal nanocatalyst,and may shed some light on the high-efficiency synthesis of other supported heterostructure with various compositions and properties.

关键词： Carbon supported non-noble catalysts Bimetal nanoparticles Hollow microspheres Catalytic hydrogenation