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Dietary nutrition, intestinal microbiota dysbiosis and post-weaning diarrhea in piglets

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Animal Nutrition 2024年第2期17卷 188-207页

作者： Xuebing han Xiangdong Hu Wei Jin gang Liu department of Urology Shengjing Hospital of China Medical University College of Bioscience and Biotechnology Hunan Agricultural University Hunan Provincial Engineering Research Center of Applied Microbial Resources Development for Livestock and Poultry State Key Laboratory of Subtropical Silviculture Zhejiang A&F University

Weaning is a critical transitional point in the life cycle of piglets. Early weaning can lead to post-weaning syndrome, destroy the intestinal barrier function and microbiota homeostasis, cause diarrhea and threaten the health of piglets. The nutritional components of milk and solid foods consumed by newborn animals can affect the diversity and structure of their intestinal microbiota, and regulate post-weaning diarrhea in piglets. Therefore, this paper reviews the effects and mechanisms of different nutrients,including protein, dietary fiber, dietary fatty acids and dietary electrolyte balance, on diarrhea and health of piglets by regulating intestinal function. Protein is an essential nutrient for the growth of piglets;however, excessive intake will cause many harmful effects, such as allergic reactions, intestinal barrier dysfunction and pathogenic growth, eventually aggravating piglet diarrhea. Dietary fiber is a nutrient that alleviates post-weaning diarrhea in piglets, which is related to its promotion of intestinal epithelial integrity, microbial homeostasis and the production of short-chain fatty acids. In addition, dietary fatty acids and dietary electrolyte balance can also facilitate the growth, function and health of piglets by regulating intestinal epithelial function, immune system and microbiota. Thus, a targeted control of dietary components to promote the establishment of a healthy bacterial community is a significant method for preventing nutritional diarrhea in weaned piglets.

关键词： Diarrhea Intestinal barrier Dietary protein Dietary fiber Dietary fatty acid Dietary electrolyte balance

Effect of austenitizing condition on mechanical properties,microstructure and precipitation behavior of AISI H13 steel

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Journal of Iron and Steel Research International 2024年第1期31卷 143-156页

作者： An-gang Ning Yang Liu Rui Gao Stephen Yue Ming-bo Wang han-jie Guo College of Materials Science and Engineering Taiyuan University of TechnologyTaiyuan030024ShanxiChina department of Mining and Materials Engineering McGill UniversityMontrealQCH3A 0C5Canada ASSAB Tooling Technology(Chongqing)Co. Ltd.Chongqing401120China School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing100083China

The effects of austenitizing temperature(1223,1303,and 1373 K)and holding time(1-1500 s)on the microstructure,mechanical properties,and precipitation behavior of the H13 hot work die steel were *** results indicate a softening phenomenon when H13 steel is austenitized at 1303 K beyond 900 s and 1373 K beyond 600 s,*** the sample held for 1200 s,the tensile strength is found capable of reaching up to 2.2 GPa when quenched from a temperature above 1303 ***,prior-austenite grain size increases with the increase in austenitizing *** kinetic behavior of the precipitates(mainly MC-type carbides)in H13 steel could be elaborated through the principles set forth by the Arrhenius and Avrami ***,the comprehensive strengthening of the H13 steel was discussed in *** results show that the activation energy of the transformed fraction of carbides is higher than that of the diffusion process for common alloying elements(Cr,V,Mo,and Ni)found in the *** suggests that it would be difficult for precipitates to dissolve into the matrix when H13 steel is austenitized at high *** the increasing austenitizing temperature,the precipitation fraction decreases,and the dislocation density *** dislocation strengthening is regarded as the dominant strengthening contributed to yield strength in as-quenched H13 steel.

关键词： H13 steel Austenitizing Mechanical property Precipitation behavior Comprehensive strengthening

Clinical evaluation of serum interleukin 10 in patients with acute pancreatitis

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Hepatobiliary & Pancreatic Diseases International 2003年第1期2卷 135-138页

作者： Xi-Chun han Ying-Chao Zhang Yu Wang Ming-Ku Jia the department of surgery Secoad hospital, jilin university, changchun 130041, china department of surgery Second Hospital Jilin University Changchun 130041 China. hanchun6001@***

OBJECTIVES: To evaluate the behavior of serum interleukin 10 (IL-10) in patients with acute pancreatitis and to explore the relationship between this cytokine and the severity of the disease. METHODS: In 45 patients with acute pancreatitis, the serum concentrations of IL-10 was determined on days 1, 2, 3, 4, 5 after admission. Twelve healthy subjects were also studied as controls. These subjects were tested using a commercial ELISA kit. The severity of pancreatitis was determined according to APACHE Ⅱ score and Balthazar CT criteria. RESULTS: Healthy subjects had no detectable serum levels of IL-10. In acute pancreatitis patients, the serum IL-10 levels were increased on the first day after the onset of the disease and then progressively decreased in the following days. On the first day after the onset of acute pancreatitis, the serum levels of IL-10 in patients with mild acute pancreatitis were significantly higher than in those with severe acute pancreatitis. In the following days, however, no statistically significant difference was observed between the two groups. CONCLUSIONS: Serum IL-10 concentration reflects the severity of acute pancreatitis. IL-10 is a useful variable for early prediction of the prognosis of acute pancreatitis. The low values of serum IL-10 in patients with severe acute pancreatitis suggests that there may be altered down-regulation of immune system response. An enhanced release of IL-10 may be a method for early treatment of acute pancreatitis.

关键词： interleukin 10 acute pancreatitis

NUMERICAL COMPUTATION OF BOUNDED SOLUTIONS FORA SEMILINEAR ELLIPTIC EQUATION ON AN INFINITE STRIP

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Journal of Computational Mathematics 1999年第2期17卷 159-170页

作者： Fu-ming Ma(department of Mathematics, jilin ’university, changchun 130023, china) 吉林大学数学系吉林长春

In this paper, we consider the computation of bounded solutions of a semilinear elliptic equation on an infinite strip. The dynamical system approach and reduction on center manifold are used to overcome the difficult... 详细信息

关键词： numerical method nonlinear PDE center manifold

Alloy as advanced catalysts for electrocatalysis:From materials design to applications

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Chinese Chemical Letters 2024年第6期35卷 104-122页

作者： Pingfan Zhang Shihuan Hong Ning Song Zhonghui han Fei Ge gang Dai Hongjun Dong Chunmei Li Advanced Chemical Engineering Laboratory of Green Materials and Energy of Jiangsu Province Institute of Green Chemistry and Chemical TechnologySchool of Chemistry and Chemical EngineeringJiangsu UniversityZhenjiang 212013China Daqo Group Co.Ltd. Zhenjiang 212221China Key Laboratory of Groundwater Resources and Environment Ministry of EducationJilin Provincial Key Laboratory of Water Resource and EnvironmentCollege of New Energy and EnvironmentJilin UniversityChangchun 130021China

With the deep integration of electrochemical research with energy,environment,catalysis,and other fields,more and more new electrochemical catalytic reactions have entered our research *** catalysts have recently emerged as a new type of nanomaterial due to the rapid development of kinetic controlled synthesis *** materials offer several advantages over monometallic catalysts,including larger element combinations,complex geometries,bifunctional sites,and reduced use of precious *** paper provides a review of alloy electrocatalysts that are designed and prepared specifically for electrocatalytic *** use of alloy materials in electrocatalyst design is also discussed,highlighting their widespread application in this ***,various synthesis methods and synthesis mechanisms are systematically *** that,by correlating the properties of materials with the structure,relevant strategies toward advanced alloy electrocatalysts including composition regulation,size,morphology,surface engineering,defect engineering,interface engineering and strain engineering are *** addition,the important electrocatalytic applications and mechanisms of alloy electrocatalysts are described and ***,the current challenges and prospects regarding the development of alloy nanomaterials are *** review serves as a springboard from a fundamental understanding of alloy structural dynamics to design and various applications of electrocatalysts,particularly in energy and environmental sustainability.

关键词： Alloy Nanomaterial Electrocatalyst Materials design Electrocatalysis

Labeling of human insulin-like growth factor-I eukaryotic expression vector with green fluorescent protein

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Chinese Journal of Traumatology 2005年第3期8卷 132-137页

作者：刘一张绍昆吴宏department of Ophthamology second hospital jilin university changchun 130041 china 单玉兴王刚徐莘香 department of Orthopaedic surgery First Hospital Jilin University Changchun 130021China

Objective: To label human insulin-like growth factor-I （hIGF-I） eukaryotic expression vector with green fluorescent protein （GFP） for the repair of articular cartilage defects. Methods: GFP cDNA was inserted into pcDNA3.1-hIGF-1 to construct the co-expression vector with two multiple cloning sites mammalian expression vector under two cytomegalovirus promoters/enhancers respectively. Recombinant pcGI was transfected into NIH 3T3 cells with the help of lipofectamine. Results: Enzyme digestion and agarose gel electrophoresis analysis revealed that pcGI vector contained correct GFP and hIGF-I cDNA. Expression of （hIGF-1） and GFP was confirmed in transfected NIH 3T3 cells by immunocytochemical analysis and fluorescence microscopy. Conclusions: hIGF-I eukaryotic expression vector has been successfully labeled with GFP.

关键词： Insulin-like growth factor I Transgenes Green fluorescent protein

Calcium Overload Is A Critical Step in Programmed Necrosis of ARPE-19 Cells Induced by High-Concentration H_2O_2

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Biomedical and Environmental Sciences 2010年第5期23卷 371-377页

作者： GUANG-YU LI BIN FAN YONG-CHEN ZHENG department of Ophthalmology Second Hospital of Jilin University Changchun 130041 Jilin China

Objective Oxidative stress plays an important role in retinal pigmental epithelium （RPE） death during aging and the development of age-related macular *** early reports indicate that reactive oxygen species （ROS） including H2O2 can trigger apoptosis at lower concentrations and necrosis at higher concentrations,the exact molecular mechanism of RPE death is still *** purpose of this study was to investigate the molecular pathways involved in RPE death induced by exogenous ROS,especially at higher *** Cultured ARPE-19 cells were treated with H2O2 at different concentrations and cell viability was measured with the MTT *** death was morphologically studied by microscopy using APOPercentage assay and PI ***,the impact of oxidative stress on ARPE-19 cells was assessed by HO-1 and PARP-1 Western blotting and by the protection of antioxidant *** influx was determined using the fura-2 calcium indicator and the role of intracellular calcium overload in ARPE-19 cell death was evaluated following cobalt treatment to block calcium *** H2O2 reduced the viability of ARPE-19 cells in a concentration-dependent manner,which was presented as a typical s-shaped *** death caused by high concentrations of H2O2 was confirmed to be programmed ***,dying ARPE-19 cells were extremely swollen and lost the integrity of their plasma membrane,positively detected with APOPercentage assay and PI staining.24-hour treatment with 500 ？mol/L H2O2 induced remarkable up-regulation of HO-1 and PARP-1 in ARPE-19 ***,antioxidant treatment using EGCG effectively protected cells from H2O2-induced injury,increasing cell viability from 14.17%±2.31% to 85.77%±4.58%.After H2O2 treatment,intracellular calcium levels were highly elevated with a maximum concentration of ***,the calcium channel inhibitor cobalt was able to blunt this calcium influx and blocked the necrotic

关键词： Apoptosis ARPE-19 cell Necrosis Oxidative-stressed injury Hydrogen peroxide

Kernel model-based diagnosis

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Progress in Natural Science:Materials International 2002年第2期12卷 63-67页

作者： OUYANG Dantong(department of Computer Science, jilin university, changchun 130012, china) jilin Univ Dept Comp Sci Changchun 130012 Peoples R China

The methods for computing the kernel consistency-based diagnoses and the kernel abductive diagnoses are only suited for the situation where part of the fault behavioral modes of the components are known. The characterization of the kernel model-based diagnosis based on the general causal theory is proposed, which can break through the limitation of the above methods when all behavioral modes of each component are known. Using this method, when observation subsets deduced logically are respectively assigned to the empty or the whole observation set, the kernel consistency-based diagnoses and the kernel abductive diagnoses can deal with all situations. The direct relationship between this diagnostic procedure and the prime implicants/implicates is proved, thus linking theoretical result with implementation.

关键词： model-based diagnosis general causal theory prime implicant/implicate diagnostic space.

COMPARISON　THEOREMS　TO　BOUNDARY　VALUE　PROBLEMS　FOR　ORDINARY　DIFFERENTIAL　EQUATIONS

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Chinese Annals of Mathematics,Series B 1994年第2期15卷 165-172页

作者： LI YONG WANG HUAIZHONG(department of Matehematics,jilin university, changchun 130023, jilin, china) jilin UNIV DEPT MATHCHANGCHUN 130023PEOPLES R CHINA

A unified approach is given to several classes of nonlinear boundary value problems, and some previous existence theorems are extended.

关键词： Boundary value problem Existence theorem Brouwer degree,Schauder's fixed point theorem.