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Molecular identification and in vitro interaction of molds associated with dry rot of potato(Solanum tuberosum L.)collected in La Trinidad,Benguet,Philippines

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Studies in Fungi 2021年第1期6卷 315-326页

作者： Carreon GAD gandalera ee Waing KGD Department of Biological Sciences College of ScienceCentral Luzon State UniversityScience City of MuñozNueva EcijaPhilippines 3120

Solanum tuberosum *** the Solanaceae family is one of the most important agricultural crops grown in the Philippines next to ***,harvested potatoes were plagued with various fungal diseases resulting to occurrence of dry rot affecting the production and harvest of *** rot in potatoes is caused by several fungal species in the genus ***,this study aimed to isolate and identify fungal species associated with dry rot disease of *** showing diseased symptoms of dry rot were randomly selected and collected from various traders in La Trinidad,Benguet and were subjected for fungal isolation through serial dilution and *** was done through observation of cultural and morphological characteristics as well as DNA barcoding using ITS region.A total of six fungal species were subjected into BLAST which revealed the following species:Aspergillus flavus(100.00%),***(100.00%),***(99.82%),Fusarium oxysporum(95.06%),***(100.00%)and Mucor velutinosus(96.45%).To establish possible fungal species as potential biocontrol agent that would lessen the use of harmful chemicals,the identified fungal organisms were interacted with one another through in vitro *** showed that fungi exhibited antagonistic interaction against each *** denaturation,hyphal penetration,hyphal coiling and cell lysis,were observed through microscopic ***,*** showed the ability to penetrate the host hyphae of the *** and ***,Mucor velutinosus could be a potential biological control agent against Fusarium species,which can reduce the use of harmful fungicides in controlling fungal diseases in potato that causes dry rot.

关键词： antagonism fungal diseases hyphal interaction Mucor velutinosus

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