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Effect of melatonin and/or cysteamine on development and vitrification of buffalo embryos

Asian pacific Journal of Reproduction 2017年第4期6卷 176-180页

作者： Mohamed MM El-Sokary Mohamed El-Raey Karima GhM Mahmoud Mahmoud EA Abou El-Roos gamal ms sosa Department of Theriogenology Faculty of Veterinary MedicineBenha UniversityP.O:13736TokhKaliobiaEgypt Department of Anim.Reprod.&A.I. National Research CenterDokkiGizaEgypt

Objective:To assess the effects of melatonin and/or cysteamineonin vitro maturation, culturing and post-warming of buffalo ***: Buffalo oocytes were classified into control, cysteamine (50 μM), melatonin (10 ng/mL) and cysteamine (50 μM) + melatonin (10 ng/mL) treatment groups. In experiment 1, previous treatments were added duringin vitromaturation and culturing of buffalo ***:Cleavage and blastocyst rates were significantly (P<0.05) increased in melatonin treated group (70.5±0.9 and 12.8±1.0, respectively). However this effect was potentiated when combined with cysteamine (74.0±1.7 and 14.8±1.7, respectively). In experiment 2, the treatements were added in maturtaion, culturing as well as post-warming culture media. Embryos at 7 d were *** assessement directly after warming showed significant increase (P<0.05) in cysteamine, melatonin and their combination groups (76.8±2.8, 80.0±2.1 and 83.3±1.7, respectively) than control (65.8±2.4);but the viability after 24 h post-warming was the best in cysteamine + melatonin combination group (61.4±2.1).Conculsions: Enriching maturation, culturing and post-warming media of buffalo oocytes and embryos with melatonin and/or cysteamine have significantly beneficial effects on oocyte developmental competence as well as embryos vitrification procedure outcomes which in turn resulting in enhancement of commercial buffalo embryo production.

关键词： Melatonin Cysteamine Buffalo Oocyte Maturation Vitrification

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Effect of using ascorbic acid and cysteamine supplementation onin-vitrodevelopment of buffalo embryos

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Asian pacific Journal of Reproduction 2017年第2期6卷 85-88页

作者： Al-shimaa Al-H.H.El-Naby Karima Gh.M.Mahmoud gamal A.M.sosa Mahmoud E.A.Abouel-Roos Youssef F.Ahmed Department of Theriogenology Faculty of Veterinary MedicineBenha UniversityEgypt Department of Animal Reproduction and A.I. National Research CenterDokkiGizaEgypt

Objective:To improvein vitro embryo production in buffalo by supplementation of L-ascorbic acid during maturation and development (experiment 1) and combination with another antioxidant as cysteamine (experiment 2).Methods:Two experiments were performed, the first one aimed to evaluate the different concentrations (0, 25, 50, 100 μM) of L-ascorbic acid on embryo developmental rate of buffalo oocytes. The L-ascorbic acid was added to the maturation and culture media. In the second experiment, oocytes were cultured in media with two type of antioxidant (ascorbic acid + cysteamine) or ascorbic acid ***:There was a significant increase in cleavage rate at 25, 50 μM than 100 μM and control group. But, the blastocyst rate was higher at 50 μM ascorbic acid than other concentrations (0, 25, 100 μM). Supplementation of ascorbic acid and cysteamine to maturation and cultured media improved embryo development than ascorbic acid ***: Using of 50 μM L-ascorbic acid duringin vitro maturation and development improve the developmental competence of buffalo oocytes, this effect was increase with the presence of cysteamine.

关键词： Buffalo Oocytes L-ascorbic acid Cysteamine Embryo development

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