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Simvastatin Action Is Not Related to HDAC2 Expression in Non-Small Cell Lung Cancer (NSCLC)

Journal of Cancer Therapy 2016年第12期7卷 939-952页

作者： gallelli luca Falcone Daniela Perri Mariarita Erika Cione Pelaia Girolamo Mesuraca Maria Terracciano Rosa Spaziano Giuseppe D’Agostino Bruno Navarra Michele Savino Rocco Department of Health Science University of Catanzaro Catanzaro Italy Department of Pharmacy Health and Nutritional Science University of Calabria Cosenza Italy Department of Medical and Surgical Sciences University of Catanzaro Catanzaro Italy Department of Experimental Medicine School of Medicine Second University of Naples Naples Italy Department of Drug Sciences and Health Products University of Messina Messina Italy

In this study, we lowered the expression of HDAC2 protein, to evaluate the effects of simvastatin on the biochemical pathways involved in inflammatory and metastatic response. The model used is the non-small cell lung cancer line (GLC-82). Trypan blue staining for assessing vital cell number to be seed and MTT assay was used as cell proliferation test. Lentivus for HDAC2 was used to silence its mRNA. Western blotting analysis was used for protein extracts, and ELISA was done on culture media for cytokines (IL-6, IL-8 and TNF-alpha) release. Hydrogen peroxide (H2O2) was used to induce oxidative stress. Our results have shown that Lentivirus containing the shHDAC2 in GLC-82 cells was able to reduce protein expression of HDAC2. In the GLCshHDAC2 cell line obtained, H2O2 induced a significant increase in cytokines release and ERK1/2 phosphorylation (P 0.01);a significant decrease of RECK activation (P 0.01);a significant increased activation (P 0.01) of both MMP-2 and MMP-9 and an increased activation of NF-κB, MyD88, TRAF-6, TRADD, TRAF-2. In GLCshHDAC2 cell, the treatment with simvastatin (30 μM), significantly affected all the biochemical markers examinated (P 0.01). In conclusion, from our report emerge, that simvastatin is able per se to inhibit oxidative stress in lung cancer cells, overcoming HDAC2 expression.

关键词： Lung Adenocarcinoma Cells HDAC2 Statins Cell Proliferation Cytokines

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Effects of calibration process on the simulation of rear-end conflicts at roundabouts

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Journal of Traffic and Transportation Engineering(English Edition) 2019年第2期6卷 175-184页

作者： Vincenzo gallelli Giuseppe Guido Alessandro Vitale Rosolino Vaiana Department of Civil Engineering University of Calabria

A methodology for calibrating and validating VISSIM simulation model is presented that allows to replicate the observed vehicles conflicts. A roundabout case study has been selected to test the usefulness of a combined approach of VISSIM simulation package and the surrogate safety assessment model(SSAM) for providing reliable estimates of traffic conflicts. Safety performance has been assessed from the field by video-recording vehicle interactions at the roundabout, and then expressed in terms of time to collision(TTC)*** proposed calibration procedure has been performed by a multistage methodology involving microscopic drivers' car following behavior parameters to enhance the correlation between observed and simulated queue lengths at the roundabout's entries. The calibration procedure is based on a statistical screening of inputs leading to a linear expression relating significant parameters to the queue length. The best estimates of the model's parameters have been determined using a genetic algorithm *** spatial distribution of the rear-end conflicts and the TTC values determined by SSAM have been finally compared with the observed ones to analyze the capability of the model of replicating rear-end *** results suggest to this calibration procedure impacts positively on the estimate of the safety performance measures obtained through the simulation *** the good results in the evaluation of the model's accuracy, the simulation seems to fail in reproducing the traffic phenomena linked to unusual driving behavior, and therefore it is not able to replicate forced drivers' maneuvers that can lead to a conflict situation.

关键词： Simulation Safety performance Unmanned aerial vehicle Roundabouts Traffic conflicts

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中国梦理论：中国国家话语的新形态

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学术界 2017年第2期 136-144页

作者： [意]Beatrice gallelli 北京大学新闻与传播学院北京100871 威尼斯卡福斯卡里大学意大利威尼斯30123

"中国梦"是当代中国最高领导人对近现代中国社会建构理想的新概括,这既是一种政治理论,同时也体现了中国国家话语的新趋向。"中国梦"富有深刻的修辞内涵和全球意义,投射了中国的国家价值取向,引领了中国在国际舞台上的走向。从学理角度... 详细信息

"中国梦"是当代中国最高领导人对近现代中国社会建构理想的新概括,这既是一种政治理论,同时也体现了中国国家话语的新趋向。"中国梦"富有深刻的修辞内涵和全球意义,投射了中国的国家价值取向,引领了中国在国际舞台上的走向。从学理角度阐释这一国家隐喻的修辞建构,有利于理解当代中国及其在全球话语体系中的角色。本文试图从国家传播学、国家修辞学、国家话语学等视角探索"中国梦"的概念意义、文化意义及其在中国国家话语体系和全球话语体系建构中的地位和作用。本文认为,"中国梦理论"体现了中国政治话语向民间话语的转型,拓展了政治话语在民间的话语空间,在国际上体现了当代中国最高领导人的全球性思维。

关键词： '中国梦' 修辞国家意象话语

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