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Monitoring the.autoprote.lysis of hiv-1 prote.se.by site.dire.te. spin-labe.ing and e.e.tron paramagne.ic re.onance.spe.troscopy

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Journal of Biophysical Che.istry 2011年第2期2卷 137-146页

作者： Jamie.L. Ke.r Luis Galiano Ange.o M. Ve.oro Laura S. Buse.le.ne. gail e. fanucci De.artme.t of Che.istry University of Florida Gainesville Florida USA

Site.dire.te. spin-labe.ing with continuous wave.e.e.tron paramagne.ic re.onance.spe.troscopy was use. to monitor autoprote.lysis of HIV-1 prote.se. an e.zyme.e.se.tial for viral maturation. Two prote.n constructs we.e.e.amine., name.y subtype.F and the.circulating re.ombinant form CRF01_A/e. As the.prote.se.unde.goe. se.f-cle.vage. prote.n unfolds and small pe.tide.fragme.ts containing the.spin labe. are.ge.e.ate., which colle.tive.y give.rise.to a sharp spe.tral compone.t that is e.sily disce.nable.in the.high-fie.d re.onance.line.in the.e.R spe.trum. By monitoring the.inte.sity of this spe.tral compone.t ove. time. the.autoprote.lytic stability of e.ch construct was characte.ize. unde. various conditions. Data we.e.colle.te. for sample. store. at 4 °C, 25 °C, and 37 °C, and on a subtype.F HIV-1 prote.se.sample.store. at 25 °C and containing the.FDA-approve. prote.se.inhibitor Tipranavir. As e.pe.te., the.rate.of autoprote.lysis de.re.se. as the.storage.te.pe.ature.was lowe.e.. Minimal autoprote.lysis was se.n for the.sample.that containe. Tipranavir, providing dire.tion for future.spe.troscopic studie. of active.prote.se.sample.. Whe. compare. to standard me.hods of monitoring prote.n de.radation such as ge. e.e.trophore.is or chromatographic analyse., spin-labe.ing with CW e.R offe.s a facile. re.l-time. non-consuming way to monitor autoprote.lysis or prote.n de.radation. Additionally, mass spe.trome.ry studie. re.e.le. that the.N-te.mini of both constructs are.se.sitive.to de.radation and that the.site. of spe.ific autoprote.lysis vary.

关键词： HIV-1 Prote.se Autoprote.lysis Se.f-Prote.lytic Activity Site.Dire.te. Spin-Labe.ing e.e.tron Paramagne.ic Re.onance (e.R) Spe.troscopy

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Language.arts : patte.ns of practice 6th ed.

Language arts : patterns of practice

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2005年

作者： Tompkins gail e.

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The.Pre.e.ce.but not the.Location of an Appe.dicolith Affe.ts the.Succe.s of Inte.val Appe.de.tomy in Childre. with Rupture. Appe.dicitis

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Surgical Scie.ce 2011年第2期2卷 39-44页

作者： Iyore.A. Jame. Ste.he. Druhan Donna A. Caniano gail e. Be.ne. 不详

Purpose. To de.e.mine.whe.he. the.pre.e.ce.and/or location of an appe.dicolith can pre.ict failure.of initial non-ope.ative.manage.e.t in childre. with rupture. appe.dicitis. Me.hods: A re.rospe.tive.chart re.ie. of pe.iatric patie.ts pre.e.ting with rupture. appe.dicitis was pe.forme.. Patie.ts in whom the.inte.t to tre.t was with initial non-ope.ative.manage.e.t and inte.val appe.de.tomy (IA) we.e.se.e.te. (n = 117). One.patie.t was e.clude. due.to the.pre.e.ce.of both intraluminal and e.traluminal appe.dicoliths. Childre. who faile. initial non-ope.ative.manage.e.t we.e.assigne. to the.“failure. group (n = 22). Childre. that improve. and unde.we.t e.e.tive.IA we.e.assigne. to the.“succe.s” group (n = 94). Age. ge.de., duration of symptoms, pre.e.ce.of an appe.dicolith, appe.dicolith location (intraluminal/e.traluminal), pre.e.ce.of a drainable.absce.s, and complications we.e.re.ie.e.. Re.ults: The.e.was an ove.all 18.8% failure.rate.for IA. Patie.ts with an appe.dicolith had a 41.7% failure.rate. and patie.ts without an appe.dicolith had a 13% failure.rate.(p = 0.003). Patie.ts with intraluminal or e.traluminal appe.dicoliths e.ch had a 41.7% failure.rate. The.pre.e.ce.or abse.ce.of a drainable.in-tra-abdominal absce.s did not affe.t the.failure.rate. Childre. in the.failure.group pre.e.te. to the.hospital e.rlie. (6.57 ± 2.59 vs. 10.02 ± 7.21 days;p = 0.030). Conclusions: The.pre.e.ce.of an appe.dicolith incre.se. the.like.ihood of failure.of initial non-ope.ative.manage.e.t of rupture. appe.dicitis in childre.;howe.e., the.location of the.appe.dicolith is not a pre.ictor of failure.

关键词： Rupture. Appe.dicitis Failure Non-Ope.ative Manage.e.t Appe.dicolith

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Role. of the.e.e.care.workforce.for task sharing in manage.e.t of diabe.ic re.inopathy in Cambodia

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Inte.national Journal of Ophthalmology(e.glish e.ition) 2018年第1期11卷 101-107页

作者： Mufarriq Shah gail M.Ormsby Aye.ha Noor Rahul Chakrabarti Manfre. Morche. Fakir MAmirul Islam CAle. Harpe. Jill e.Ke.ffe Pakistan Institute.of Community Ophthalmology Hayatabad Me.ical Comple. Peshawar 25000Pakistan Ce.tre.for e.e.Re.e.rch Australia Department of Ophthal-mologyUniversity of MelbourneRoyal Victorian Eye and Ear HospitalMelbourneVIC 302Australia Faculty of e.ucation Science and BusinessLifestyle Research CentreAvondale College of Higher EducationCooranbongNSW 2265Australia Vision and De.tal Care.Clinic Peshawar 25000Pakistan CARITAS Take. e.e.Hospital Krong Doun Kaev 21151Cambodia Re.ional office.Asia South e.st CBM Inte.national Unit 604 Alabang Busine.s Towe. 1216 Acacia AveMuntinlupa City 1799Philippines Statistics Data Science and EpidemiologySwinburne University of TechnologyHawthornVIC 3122Australia LV Prasad e.e.Institute Hyderabad 500034India

AIM： To ide.tify the.curre.t role. of e.e.and he.lth care.worke.s in e.e.care.de.ive.y and inve.tigate.the.r pote.tial role. in scre.ning and de.e.tion for manage.e.t of diabe.ic re.inopathy（DR） through task ***： Purposive.sampling of 24 participants including he.lth administrators, me.be.s from non-gove.nme.t organizations and all available.e.e.care.worke.s in Take. province.we.e.re.ruite.. This cross se.tional mixe. me.hod study comprise. a surve. and in-de.th inte.vie.s. Data we.e.colle.te. from me.ical re.ords at Caritas Take. e.e.Hospital（CTe.） and Kiri Vong District Re.e.ral Hospital Vision Ce.tre. and a surve. and inte.vie.s with participants we.e.done.to e.plore.the.pote.tial role. for task sharing in DR manage.e.t. Qualitative.data we.e.transcribe. into a te.t program and the. e.te.e. into N-Vivo（ve.sion 10） software.for data manage.e.t and analysis. Re.ULTS： From 2009 to 2012, a total of 105 178 patie.ts we.e.e.amine. and 14 030 e.e.surge.ie. we.e.pe.forme. in CTe. by thre. ophthalmologists supporte. by ophthalmic nurse. in ope.ating and e.e.e.amination for patie.ts. Be.we.n January 2011 and Se.te.be. 2012, 151 patie.ts（72 male.） with re.inal pathology including 125（83%） with DR visite. CTe.. In addition 170 patie.ts with diabe.e. we.e.re.e.re. to CTe. for e.e.e.aminations from Mo Po Tsyo scre.ning programs for pe.ple.with diabe.e.. Factors favouring task sharing include. high de.and for e.e.care.se.vice. and scarcity of ***： Task sharing and te.m work for e.e.care.se.vice. is functional. Participants favor the.pote.tial role.of ophthalmic nurse. in scre.ning for DR through task sharing.

关键词： task sharing diabe.e. diabe.ic re.inopathy human re.ource. Cambodia

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Re.iprocal Association be.we.n Psychological Distre.s and PTSD and The.r Re.ationship with Pre.Displace.e.t Stre.sors among Displace. Wome.

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Inte.national Journal of Me.tal He.lth Promotion 2023年第5期25卷 699-710页

作者： e.habor S.Ide.udia Babatola D.Olawa gail e.Wyatt Norwe.ta G.Milburn Faculty of Humanitie. North-West UniversityMafikeng Campus34151South Africa De.artme.t of Psychiatry&Be.avioural Scie.ce. Jane&Terry Semel Institute for Neuroscience&Human BehaviourUniversity of California Los AngelesLos Angeles90095USA

It is e.tablishe. in the.psychological lite.ature.that pre.displace.e.t stre.sors,PTSD symptoms,and psycholo-gical distre.s are.associate. among inte.nally displace. ***,e.isting studie. have.not de.onstrate. the.me.hanism unde.lying the.e.*** study compare. two e.planatory mode.s;one.with PTSD symptoms se.e.ity e.plaining the.indire.t association be.we.n pre.displace.e.t stre.sors and psychological dis-tre.s,and the.othe. with psychological distre.s e.plaining the.indire.t re.ationship be.we.n pre.displace.e.t stre.sors and PTSD symptoms *** a cross-se.tional de.ign,631 wome.(Me.n age.31.18±8.59)we.e.conve.ie.tly and purpose.y se.e.te. from the.displace. wome. harboure. in two camps in Borno State.Nige.ia,due.to the.Boko-Haram *** we.e.colle.te. utilizing structure. que.tionnaire. and subje.te. to path *** de.onstrate. that PTSD symptoms se.e.ity and psychological distre.s indire.tly e.plaine. the.r re.pe.tive.and inde.e.de.t association with pre.displace.e.t ***,PTSD symptoms had a statis-tically large. standardize. indire.t e.fe.t size.gre.te. indire.t e.fe.t pe.ce.tage.and bigge. kappa-square.(k2)e.fe.t size.than psychological *** symptoms and psychological distre.s may have.a re.iprocal inﬂue.ce.on e.ch othe. from pre.displace.e.t ***,PTSD symptoms can be.te. e.plain the.association be.we.n pre.displace.e.t stre.sors and psychological distre.s than the.othe. way *** outcome. have.important implications for the.psychological tre.tme.t of displace. pe.sons.

关键词： PTSD symptoms psychological distre.s traumatic stre.s inte.nally displace. pe.sons

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<i>Stachybotrys chartarum</i>(<i>atra</i>) spore.e.tract alte.s surfactant prote.n e.pre.sion and surfactant function in isolate. fe.al rat lung e.ithe.ial ce.ls, fibroblasts and human A549 ce.ls

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Ope. Journal of Pe.iatrics 2013年第3期3卷 243-256页

作者： gail F. Pollard Anthony Shaw Michae. Sowa Thomas Rand Jame. A. Thlive.is Jame. e. Scott De.artme.t of Biology St. Mary’s University Halifax Nova Scotia De.artme.t of Human Anatomy and Ce.l Scie.ce University of Manitoba Winnipeg Canada De.artme.t of Oral Biology University of Manitoba Winnipeg Canada National Re.e.rch Council Biodiagnostics Institute Winnipeg Canada

Moulds, notably Stachybotrys chartarum (atra), are.constant contributors to air pollution particularly to air quality in buildings. The.spore. the.se.ve. or the.r volatile.organic products are.pre.e.t in variable.amounts in almost all e.vironme.ts, particularly in buildings affe.te. by flooding. The.e.moulds and products can account for the.sick building syndrome.and have.be.n tie. to such occurre.ce. as the.outbre.k of pulmonary he.oside.osis and he.orrhage.in infants in Cle.e.and, Ohio. The.pre.e.t study was de.igne. to inve.tigate.the.e.fe.ts of S. chartarum e.tracts on surfactant prote.n e.pre.sion, surfactant quality and ce.l survival in the.de.e.oping lung. S. chartarum e.tracts we.e.incubate. with culture. of se.e.al ce.l type.;isolate. fe.al lung type.II ce.ls and fe.al lung fibroblasts, and human lung A549 ce.ls, a continuously growing ce.l line.de.ive. from surfactant producing type.II alve.lar ce.ls. MTT formazan assays we.e.e.ploye. to te.t ce.l viability. The.synthe.is and re.e.se.of the.pre.ominant surfactant prote.n A (SP-A), which is involve. in the.re.ulation of surfactant turnove. and me.abolism, and surfactant prote.n B (SP-B) involve. in shuttling phospholipids be.we.n surfactant subcompartme.ts was also asse.se.. Antibodie. to the.e.prote.ns and we.te.n blotting re.ults we.e.use. to asse.s the.quantity of prote.n produce. by the.various ce.l type.. A nove. approach utilizing captive.bubble.surfactome.ry was e.ploye. to inve.tigate.the.quality of surfactant in te.ms of surface.te.sion and bubble.volume.me.sure.e.ts. e.e.tron microscopy was use. to e.amine.change. in ce.lular structure.of control and S. chartarum-tre.te. ce.ls. Re.ults of the.study showe. that e.posure.to the.S. chartarum e.tracts had de.e.e.ious e.fe.ts on fe.al lung e.ithe.ial ce.l viability and the.r ability to produce.pulmonary surfactant. S. chartarum e.tracts also induce. de.e.e.ious change. to the.de.e.oping fe.al lung ce.ls in te.ms of e.pre.sion of SP-A and SP-B as we.l a

关键词： Fe.al Lung Surfactant Surface Te.sion Black Mould Captive Bubble Surfactome.e. Surfactant Prote.ns

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Pote.t and conditional re.ire.te. T ce.l killing of tumor ce.ls using Half DVD-Ig

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Prote.n & Ce.l 2018年第1期9卷 121-129页

作者： Philip D. Bardwe.ll Matthe. M. Staron Junjian Liu Qingfe.g Tao Susanne.sce.ne. gail Bukofze. Luis e. Rodrigue. Che.-Ho Choi Je.nife. Wang Qing Chang Fe.g Dong Che.rie.Donawho Jie.i Wang Christine.M. Grinne.l e.it Tarcsa Charle. Hutchins Tariq Ghayur Jijie.Gu Foundational Immunology AbbVie Bioresearch Center Worcester MA 01605 USA Global Pre.linical Safe.y AbbVie Bioresearch Center Worcester MA 01605 USA Oncology Discove.y AbbVie Inc. North Chicago IL 60064 USA DMPK-BA AbbVie Bioresearch Center Worcester MA 01605 USA Re.e.rch and De.e.opme.t AbbVie Inc. North Chicago IL 60064 USA Torque.The.ape.tics Inc. Cambridge MA 02142 USA Innove.t Bioloqics Inc. Suzhou China Lyvge. Biopharma Shanghai 201203 China

Nove. biologics that re.ire.t cytotoxic T lymphocyte. （CTLs） to kill tumor ce.ls be.ring a tumor associate. antige. hold gre.t promise.in the.clinic. Howe.e., the.ability to safe.y and pote.tly targe. CD3 on CTL toward tumor associate. antige.s （TAA） e.pre.se. on tumor ce.ls re.ains a challe.ge.of both te.hnology and biol- ogy. He.e.n we.de.cribe.the.use.of a Half DVD-Ig format that can re.ire.t CTL to kill tumor ce.ls. Notably, Half DVD-Ig mole.ule. that are.monovale.t for e.ch spe.i- ficity de.onstrate. re.uce. non-spe.ific CTL activation and conditional CTL activation upon binding to TAA compare. to intact te.ravale.t DVD-Ig mole.ule. that are.bivale.t for e.ch spe.ificity, while.maintaining good drug like.prope.tie. and appropriate.PK prope.tie..

关键词： DVD-Ig Half DVD-Ig Halt'body e.ide.mal growth factor re.e.tor re.ire.te. T-ce.l cytotoxicity rCTL

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CD8 T ce.l re.ponse.in a phase.I study of the.ape.tic vaccination of advance. NSCLC with alloge.e.c tumor ce.ls se.re.ing e.doplasmic re.iculum-chape.one.gp96-Ig-pe.tide.comple.e.

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Advance. in Lung Cance. 2013年第1期2卷 9-18页

作者： Luis e. Rae. gail R. Walke. Paule.te.Baldie.e.a Fishe. Jorge.e. Gome. Khale. Tolba e.gardo S. Santos e.khard R. Podack De.artme.t of Me.icine Division of Hematology/Oncology University of Miami Miller School of Medicine Miami USA De.artme.t of Microbiology and Immunology University of Miami Miller School of Medicine Miami USA Sylve.te. Compre.e.sive.Cance. Ce.te. Biostatistics Core Facility University of Miami Miller School of Medicine Miami USA

Antige. containing, alloge.e.c ce.ls se.re.ing the.ge.e.ically modifie. prote.n and pe.tide.chape.one.gp96-Ig cross, prime.and e.pand antige. spe.ific CD8 T ce.ls with the.ape.tic antitumor activity in mice. In a first in man phase.I study, we.now re.ort the.re.ults of the.ape.tic vaccination of non-small ce.l lung cance. (NSCLC) patie.ts with an e.tablishe., alloge.e.c non-small ce.l lung ade.ocarcinoma ce.l line.se.re.ing gp96-Ig. Advance. NSCLC-patie.ts stage.IIIB or IV of any histological subtype.we.e.e.rolle. and tre.te. with up to 36 vaccinations ove. the.course.of 18 we.ks. Primary e.dpoint was safe.y, se.ondary e.dpoints tumor re.ponse.and ove.all survival. Me.sure.e.t of tumor antige. spe.ific CD8 CTL re.ponse. is pre.lude. by the.lack of known NSCLC associate. antige.s. The.e.ore. we.me.sure. patie.t CD8 T ce.l-IFN-γ re.ponse. to allo-antige.s of the.vaccine.ce.ls as surrogate.for tumor antige. spe.ific CD8 CTL. In 7 of 18 tre.te. patie.ts tumor growth was stabilize., howe.e. none.of the.18 patie.ts had an obje.tive.tumor re.ponse.by Re.IST crite.ia. Of 15 patie.ts e.aluable.for immune.re.ponse. 11 re.ponde. to vaccination with more.than twofold incre.se.in CD8-IFN-γ fre.ue.cy above.base.ine. The.e.patie.ts had a me.ian survival time.of 16.5 months. Four patie.ts who had no CD8 re.ponse.above.base.line.had survival time. from 2.1 to 6.7 months. Our data are.consiste.t with the.conce.t that ge.e.ation of CD8 CTL by the.ape.tic vaccination may de.ay tumor growth and progre.sion and me.iate.prolonge. survival e.e. in the.abse.ce.of obje.tive.tumor re.ponse.. Furthe. studie. will be.re.uire. to te.t this conce.t and promising re.ult.

关键词： He.t Shock Prote.n Chape.one Gp-96 Non-Small Ce.l Lung Cance. Ge.e Transfe. Immunothe.apy Alloge.e.c Vaccine Cytotoxic CD8 T Ce.ls

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Book Re.omme.dation: Advance. in Wate. Quality Control

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Journal of Wate. Re.ource.and Prote.tion 2010年第12期2卷 995-996页

作者： gail Krantzbe.g Ayse.ul Tanik 不详

Book Re.omme.dation: Advance. in Wate. Quality Control gail Krantzbe.g, Ayse.ul Tanik e. al. Scie.tific Re.e.rch Publishing, 2010 316 page. ISBN: 978-1-935068-08-2 Pape.back (US$89.00) e.book (US$89.00) Orde. online. ... 详细信息

关键词： Book Re.omme.dation Advance. in Wate. Quality Control

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Polynomial Lag-e.fe.t Mode.ling for Re.ationship be.we.n Particulate.Matte. and Re.piratory Hospital Admissions,Brisbane.Australia

Polynomial Lag-effect Modelling for Relationship between Par...

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第三届环境与职业医学国际学术研讨会

作者： gail Williams School of Population He.lth University of QueenslandHerstonQLD4006Australia

＜正＞ Although it has be.n highlighte. re.e.tly that air pollutants usually have.continuous impacts on he.lth （i.e. lag-e.fe.ts） , most of the.studie. we.e.base. on the.assumption that re.ationship be.we.n e.vironme.tal e.posure.and he.lth outcome. is line.r. Howe.e., the.assumption may be.invalid be.ause.the.biological dose.re.ponse.re.ationship is ofte. curviline.r. This study aime. to de.e.op a polynomial lag-e.fe.t mode. parame.rically to e.timate.the.association be.we.n particulate.matte. le.s than 10μmin diame.e. （PM10） and re.piratory hospital admissions（RHA） .Two-stage.ge.e.alize. additive.Poisson re.re.sion mode.s we.e.use.. In stage.I, nonparame.ric e.timation was pe.forme. to discove. an appropriate.form of the.de.e.de.ce.of RHA on

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