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Cloning and Characterization of a Novel Gene GmMF1 in Soybean (Glycine max L. Merr.)

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Agricultural Sciences in China 2011年第12期10卷 1834-1841页

作者： JIANG Wei YANG Shou-ping YU De-yue gai jun-yi Soybean Research Institute Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing 210095 P.R. China

Cytoplasmic male sterility plays an important role in utilization of crop heterosis. Screening of soybean for novel genes related to male sterility in soybean could provide a basis for studying the molecular mechanism of male sterility in plants. In this study, gene differential expressions between the cytoplasmic male-sterile line NJCMS 1A and its maintainer line NJCMS 1B in soybean were analyzed using cDNA-AFLP. A differentially expressed fragment, GmMF-T4A15, was isolated from large flower buds of NJCMS1B. By searching the soybean genomic library and PCR amplification, the cDNA fulllength sequence of 1 311 bp was obtained and named GmMF1. The expression characteristics of GmMF1 were studied by semiquantitative real-time PCR and real-time quantitative PCR. The results showed that GmMF1 was expressed highly in flower buds of NJCMS1B. The deduced protein contains 436 amino acids and shows high similarity to members of the DUF620 protein family with unknown functions in other plant species. It is predicted that the protein encoded by GmMF1 is localized in the nucleus.

关键词： soybean cytoplasmic male sterility cDNA-AFLP gene differential expressions

Genetic Diversity of Chinese Soybean mosaic virus Strains and Their Relationships with Other Plant Potyviruses Based on P3 Gene Sequences

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Journal of Integrative Agriculture 2014年第10期13卷 2184-2195页

作者： YANG Qing-hua LI Kai ZHI Hai-jian gai jun-yi Soybean Research Institute Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory of Crop Genetics and Germplasm Enhancement Ministry of Science and Technology

Soybean mosaic virus （SMV）, a member of the genus Potyvirus, is a major pathogen of soybean plants in China, and 16 SMV strains have been identified nationwide based on a former detailed SMV classification system. As the P3 gene is thought to be involved in viral replication, systemic infection, pathogenicity, and overcoming resistance, knowledge of the P3 gene sequences of SMV and other potyviruses would be useful in efforts to know the genetic relationships among them and control the disease. P3 gene sequences were obtained from representative isolates of the above-mentioned 16 SMV strains and were compared with other SMV strains and 16 Potyvirus species from the National Center for Biotechnology GenBank database. The P3 genes from the 16 SMV isolates are composed of 1041 nucleotides, encoding 347 amino acids, and share 90.7-100% nucleotide （NT） sequence identities and 95.1-100% amino acid （AA） sequence identities. The P3 coding regions of the 16 SMV isolates share high identities （92.4-98.9% NT and 96.0-100% AA） with the reported Korean isolates, followed by the USA isolates （88.5-97.9% NT and 91.4-98.6% AA）, and share low identities （80.5-85.2% NT and 82.1-84.7% AA） with the reported HZ 1 and P isolates from Pinellia ternata. The sequence identities of the P3 genes between SMV and the 16 potyviruses varied from 44.4 to 81.9% in the NT sequences and from 21.4 to 85.3% in the AA sequences, respectively. Among them, SMV was closely related to Watermelon mosaic virus （WMV）, with 76.0-81.9% NT and 77.5-85.3% AA identities. In addition, the SMV isolates and potyvirus species were clustered into six distinct groups. All the SMV strains isolated from soybean were clustered in Group I, and the remaining species were clustered in other groups. A multiple sequence alignment analysis of the C-terminal regions indicated that the P3 genes within a species were highly conserved, whereas those among species were relatively variable.

关键词： Soybean mosaic virus Potyvirus P3 gene homology analysis phylogenetic tree multiple sequence alignment

Calculation of Coronary Angiographic Total Blush in Patients with Coronary Artery Disease and its Prognostic Implication

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Chinese Medical Journal 2015年第18期128卷 2485-2490页

作者： Jing-Jing gai Lu-Yue gai Jian-jun Yan Qin-Hua Jin Department of Cardiology Chinese People's Liberation Army General Hospital Beijing 100853 China

Background： Myocardial perfusion grade （MPG） is an accepted method of evaluating myocardial perfilsion. However, it does not take into the account, the extent of the perfusion. We hypothesized that myocardial blush area times MPG （total blush） would be more accurate than simple MPG, and yield better prognostic information. Methods： About 34 patients were recruited alter they had consented to both coronary angiography （CAG） and single photon emission computed tomography （SPECT）, and divided into two groups. A special dedicated computer was employed to calculate the total blush. The CAG was performed as a conventional way. Scintigraphic technetium 99m methoxyisobutyl-isonitrile rest and stress images were evaluated quantitatively. The comparison was made between stenosis versus chronic total occlusion （CTO）, MPG 1, 2 versus MPG 3, percutaneous intervention （PC1） successful versus failure. A correlation was made between ejection traction （EF） and myocardial perfusion by MPG, total blush, SPECT, and syntax score. Results： The perfusion indices of total blush, summed difference score （SDS） and syntax score were insignificant between the two groups （P 〉 0.05）. However, the left ventricular end diastolic volume was significantly larger in CTO （P 〈 0.05）. The patients with stenosis had better MPG than with CTO （P 〈 0.05）. The increased MPG was associated with increased total blush, higher syntax score, and EF （P 〈 0.05）. Successful PCI resulted in better perfusion indicated by increased total blush, and MPG （P 〈 0.05） but successful PC1 did not change syntax score. EF and SDS significantly. Multivariate linear analysis with EF as the dependent factor and syntax score, SDS, total blush, blush area, and MPG as the independent factors showed a significantly higher degree of correlation （R = 0.87, P 〈 0.05）. Conclusion： Alter PCI the total blush and EF improved significantly indicating its potential application in the thture.

关键词： Coronary Heart Disease: Ejection Fraction Myocardial Perfusion Percutaneous Coronary Intervention: Single PhotonEmission Computed Tomography Syntax Score

QTL Identification of the Insensitive Response to Photoperiod and Temperature in Soybean by Association Mapping

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Journal of Integrative Agriculture 2013年第8期12卷 1423-1430页

作者： ZUO Qiao-mei WEN Zi-xiang ZHANG Shu-yun HOU Jin-feng gai jun-yi YU De-yue XING Han Key Laboratory of Biology and Genetic Improvement of Soybean Ministry of Agriculture/National Key Laboratory of Crop Genetics and Germplasm Enhancement/College of AgricultureNanjing Agricultural University

The insensitive response to photoperiod and temperature is an important quantitative trait for soybean in wide adaptation breeding. The natural variation in response to photoperiod and temperature was detected using 275 accessions of soybean [Glycine max （L.） Merrill] from China. Genome-wide association mapping, based on population structure analysis, was carried out using 118 SSR markers by the TASSEL GLM （general linear model） program. Nine SSR markers （P〈0.01） were associated with the value of the response to photoperiod and temperature （VRPT） caused by days to flowering （DF）, among which, Satt308 （LG M）, Sattl50 （LG M） and Satt440 （LG l）, were identified in both 2006 and 2007. Twelve SSR markers （P〈0.01） were associated with VRPT caused by days to maturity （DM）, among which three markers, Satt387 （LG N）, Satt307 （LG C2） and AW310961 （LG J）, were detected in both 2006 and 2007. In addition, a total of 20 elite alleles were screened out over 2006 and 2007 for being associated with an insensitive response to photoperiod and temperature （IRPT） caused by DF and a total of seven different elite alleles were screened out for being associated with IRPT caused by DM. Among these elite alleles, five alleles, Satt150-244, Satt308-164, Satt308-206, Satt440-176, and Satt440-206, were associated with IRPT caused by DF and were identified in both years, but only one allele, Satt307-170, was identified as being associated with an IRPT caused by DM. Based on these elite alleles, a set of typical accessions were screened out. The result about the genetic basis of IRPT is meaningful for soybean wide adaption breeding.

关键词： QTL association mapping soybean insensitive response photoperiod and temperature

Maize-soybean strip intercropping： Achieved a balance between high productivity and sustainability

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Journal of Integrative Agriculture 2018年第4期17卷 747-754页

作者： DU jun-bo HAN Tian-fu gai jun-yi YONG Tai-wen SUN Xin WANG Xiao-chun YANG Feng LIU Jiang SHU Kai LIU Wei-guo YANG Wen-yu Sichuan Engineering Research Center for Crop Strip Intercropping System/Key Laboratory of Crop Eco-physiology and Farming System in Southwest China Ministry of Agriculture/College of Agronomy Sichuan Agricultural University Chengdu 611130 P.R.China Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China National Center for Soybean Improvement Nanjing Agricultural University Nanjing 210095 P.R. China

Intercropping is one of the most vital practice to improve land utilization rate in China that has limited arable land resource. However, the traditional intercropping systems have many disadvantages including illogical field lay-out of crops, low economic value, and labor deficiency, which cannot balance the crop production and agricultural sustainability. In view of this, we developed a novel soybean strip intercropping model using maize as the partner, the regular maize-soybean strip intercropping mainly popularized in northern China and maize-soybean relay-strip intercropping principally extended in southwestern China. Compared to the traditional maize-soybean intercropping systems, the main innovation of field lay-out style in our present intercropping systems is that the distance of two adjacent maize rows are shrunk as a narrow strip, and a strip called wide strip between two adjacent narrow strips is expanded reserving for the growth of two or three rows of soybean plants. The distance between outer rows of maize and soybean strips are expanded enough for light use efficiency improvement and tractors working in the soybean strips. Importantly, optimal cultivar screening and increase of plant density achieved a high yield of both the two crops in the intercropping systems and increased land equivalent ratio as high as 2.2. Annually alternative rotation of the adjacent maize-and soybean-strips increased the grain yield of next seasonal maize, improved the absorption of nitrogen, phosphorus, and potasium of maize, while prevented the continuous cropping obstacles. Extra soybean production was obtained without affecting maize yield in our strip intercropping systems, which balanced the high crop production and agricultural sustainability.

关键词： maize soybean strip intercropping high production agricultural sustainability

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植物数量性状遗传体系的分离分析方法研究

遗传 2005年第1期27卷 130-136页

作者：盖钧镒南京农业大学国家大豆改良中心南京210095

在传统的数量性状多基因遗传模型基础上提出主基因多基因遗传模型具普遍性,纯主基因或纯多基因遗传模型只是其特例。由此初步建立了植物数量性状遗传体系分离分析方法。目前该方法可以检验2～3个主基因的个别遗传效应、多基因整体的遗... 详细信息

在传统的数量性状多基因遗传模型基础上提出主基因多基因遗传模型具普遍性,纯主基因或纯多基因遗传模型只是其特例。由此初步建立了植物数量性状遗传体系分离分析方法。目前该方法可以检验2～3个主基因的个别遗传效应、多基因整体的遗传效应和两者的遗传率。介绍这种分离分析方法的研究经过、主要进展及应用效果,并以实例说明其分析步骤、方法和效果。

关键词：植物数量性状主基因-多基因遗传模型分离分析

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酶法提取柿子皮中黄酮类物质的研究

中国检验检测 2024年第1期32卷 20-23页

作者：马永富张育生盖俊玲谭琦王珊珊刘波莱阳市检验检测中心莱阳265200

以柿子皮为原料,采用酶解法提取对柿子皮中黄酮类物质进行提取。运用单因素试验和正交试验研究酶解温度、提取时间、添加酶剂量、缓冲液pH值对黄酮提取的影响。结果表明:影响柿皮提取黄酮化合物的因素由大到小的是:pH值﹥提取时间﹥酶... 详细信息

以柿子皮为原料,采用酶解法提取对柿子皮中黄酮类物质进行提取。运用单因素试验和正交试验研究酶解温度、提取时间、添加酶剂量、缓冲液pH值对黄酮提取的影响。结果表明:影响柿皮提取黄酮化合物的因素由大到小的是:pH值﹥提取时间﹥酶剂量﹥提取温度,最高的提取参数是:pH4.8的磷酸氢二钠—柠檬酸缓冲液,70mg的复合酶,50℃的温度下提取60min。

关键词：黄酮类物质酶解法单因素试验正交试验

Gene Mapping of Brachytic Stem and Its Effect on Main Agronomic Traits in Soybean

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Agricultural Sciences in China 2005年第10期4卷 728-732页

作者： CUI Shi-you MENG Qing-chang HUANG Fang ZHAO Tuan-jie gai jun-yi YU De-yue National Center for Soybean Improvement National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 P.R.China

Brachytic stem is a major trait in plant type .of soybean and its yield potential may be higher under high population when compared with normal stem. In the present investigation, 152 recombinant inbred line （RIL） families derived from the cross of Bogao （normal stem） and Nannong 94-156 （brachytic stem） were used to map genes and QTLs of three plant type traits and to identify the effects of brachytic stem on agronomic traits such as yield. The primary results indicated that brachytic stem （sb） and determinate growth habit （drl） were mapped on linkage groups B2 and L, three major QTLs related to plant height were detected and mapped on linkage group L near drl, another minor QTL was mapped near sb on linkage group B2-1. Lines with brachytic stem had shorter plant height, lower biomass, yield, harvest index and pods per plant, and essentially no differences in days to maturity and 100-seed weight when compared with normal stem lines. It was obvious that the effect of brachytic stem on yield was due to the decreased height, biomass and harvest index.

关键词： Brachytic stem Gene mapping Quantitative trait loci (QTL) Agronomic trait Soybean

Comparison of symptom and risk assessment methods among patients with chronic obstructive pulmonary disease

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Chinese Medical Journal 2014年第14期127卷 2594-2598页

作者： Zhang Rongbao Tan Xingyu He Quanying Chen Qing gai jun Wei Jing'an Wang Yan Peking Univ Peoples Hosp Dept Resp & Crit Care Med Beijing 100044 Peoples R China

Background The global strategy for the diagnosis, management and prevention of chronic obstructive pulmonary disease （COPD） guidelines classify patients into four groups according to the number of symptoms and the level of future risk of acute exacerbation COPD （AECOPD）. This study aimed to compare the results of different methods used in diagnosis of COPD and evaluate the accuracy of the assessment methods in guiding clinical practice. Methods A survey was conducted of 194 COPD outpatients between March and September 2012. Demographic characteristics, the number of exacerbations the patient has had within the previous 12 months, COPD assessment test （CAT）, Modified British Medical Research Council （mMRC） scale, and results of the lung function tests were recorded. Results Of the 194 patients assessed, 21 had a CAT score 〉10 and an mMRC grade ≤1, 13 had a CAT score 〈10 and an mMRC grade ≥2. A predicted forced expiratory volume in one second （FEV1%） of 〈50% with less than two acute exacerbations was observed in 39 patients, while a predicted FEV1% of 〉50% was noted in 20 patients with two or more acute exacerbations. The sensitivity of a predicted FEV1% 〈50% in predicting the risk of AECOPD in the future was 80.9%, while that in the real number of AECOPD events recorded was 62.8%, the difference being statistically significant （P=0.004）. The sensitivity of CAT in predicting the severity of symptoms was 90%, while that of mMRC was 83.8%, and the difference was not statistically significant. Conclusions The COPD assessment method recommended by the global initiative for chronic obstructive pulmonary disease （GOLD） 2011 is complicated and should be simplified. CAT is more comprehensive and accurate than mMRC. The lung function classification is a better tool for predicting the risk of AECOPD in the future, and the number of AECOPD can be referred to when required.

关键词： Zhang Rongbao Tan Xingyu He Quanying Chen Qing gai jun Wei Jing'an and Wang Yan