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Effects of laminar shear stress versus resveratrol on the citrulline-NO cycle in endothelial cells

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Advances in Biological Chemistry 2013年第1期3卷 18-25页

作者： sue Im jang Yong Chool Boo Department of Molecular Medicine Cell and Matrix Research Institute BK21 Medical Education Program for Human Resources School of Medicine Kyungpook National University Daegu Korea

Laminar shear stress (LSS) due to pulsatile blood flow enhances endothelial function by multiple mechanisms including NO production. Red wine and its constituent, resveratrol, have also been postulated to provide vascular protective effects. The aim of the present study was to compare the effects of mechanical LSS and pharmacological resveratrol treatments on the endothelial citrulline-NO cycle. Human umbilical vein endothelial cells (HUVECs) were treated with LSS (12 dyn·cm-2) or resveratrol (25 - 100 μM). The expressions of argininosuccinate synthetase 1 (ASS1), argininosuccinate lyase (ASL), nitric oxide synthase 3 (NOS3) and cationic amino acid transporter 1 (CAT1), and the production of NO were determined. The expressions of Kruppel-like factor (KLF) 2 and KLF4 as upstream regulators of ASS1 and NOS3 were also analyzed. LSS strongly increased the mRNA levels of ASS1 (8.3 fold) and NOS3 (5.4 fold) without significant effects on ASL and CAT1 mRNAs. Resveratrol increased the ASS1 mRNA level in a dose-dependent manner up to 3.8 fold at 100 μM. The effects of resveratrol on the expressions of KLF2 and KLF4 mRNAs were smaller than those of LSS. Protein levels of ASS1 and NOS3, and NO production were markedly increased by LSS but resveratrol (50 μM) increased only ASS1 protein level. The results of the current study showed that LSS had greater effects on the citrulline-NO cycle activity leading to NO production, compared to resveratrol. Because resveratrol was not so effective at stimulating the endothelial citrulline-NO cycle, further studies are needed to find more potent drugs that increase the expression of ASS1 and NOS3 genes.

关键词： Citrulline-Nitric Oxide Cycle Endothelial Cells Laminar Shear Stress Resveratrol

Differences between Cheeks and Dorsal Hands Skin Properties during Skiing in a Cold and Windy Environment

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Journal of Cosmetics, Dermatological Sciences and Applications 2017年第1期7卷 27-33页

作者： sue Im jang Jiyei Choi Jiyeon Han Eun Joo Kim Hae Kwang Lee AMORE PACIFIC Corporation/R & D CENTER Yongin Korea

Background/Purpose: The skin protects against external factors and this skin function can be affected by environmental conditions, including low temperature, low humidity, and high altitude. Therefore, it is important to study the relationship between the environment and the skin. The purpose of this study is to determine how the skin barrier functions change at low temperatures. Methods: This study was performed at a Korean ski resort on January 2015 and evaluated 10 subjects skin condition during skiing. Skin properties were examined at 2 sites (cheeks and dorsal hands) and four time points (indoor;baseline, outdoor;after first skiing and after second skiing, indoor;after taking a shower). Results: The skin hydration on the cheek decreased after the second skiing session and the erythema decreased only after the first skiing while this value on dorsal hand was not changed by skiing. The brightness and yellowness of cheek skin increased and redness decreased therefore, skin color became pale during skiing. The extensibility of cheeks and dorsal hands decreased after skiing. We found positive correlation between skin temperature and redness (r2 = 0.707, p Conclusions: Low temperatures can affect human skin color so that the skin color becomes pale because capillary blood vessels shrink when skin is exposed to cold environment. The cheek skin tends to change easily than dorsal hand during ambient temperature change.

关键词： Low Temperature Environment Skiing Hydration Skin Color Elasticity Skin Temperature Skin Redness

用铼-锡催化剂氢化油酸制9-十八烯-1-醇

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表面活性剂工业 1994年第2期 20-24页

作者： Jhin sue jang 于宗顺马来西亚

证明一种新的二金属铼-锡催化剂在温和氢化条件下能够还原油酸为9-十八烯-1-醇(顺式和反式异构物)具有显著的收率。该论文报导的研究涉及催化剂制造方法,载体的类型,催化剂原料,金属的克分子比,活化和在活性上反应条件诸方面的效果以及... 详细信息

证明一种新的二金属铼-锡催化剂在温和氢化条件下能够还原油酸为9-十八烯-1-醇(顺式和反式异构物)具有显著的收率。该论文报导的研究涉及催化剂制造方法,载体的类型,催化剂原料,金属的克分子比,活化和在活性上反应条件诸方面的效果以及催化剂的选择性。在氧化铝上由过铼酸铵和四氯化锡化合衍生的催化剂性能最好,在氢化过程中锡的存在对于油酸烯键保护催化剂是必不可少的。

关键词：十八烯醇铼锡催化剂氢化油酸

Renewable Biofuel Production Using Red Ginseng Marc in Microbial Fuel Cells

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Journal of Sustainable Bioenergy Systems 2022年第1期12卷 1-11页

作者： Sung Jun jang Immanuel H. Anaborne Jacqueline E. Chang Seohyun Shim sue Min Shin Justin Kong Seunghan Baek Kunmin Kim Abraham Ha Paul S. Chung The Village School Houston USA Oakton High School Vienna USA Redondo Union High School Redondo Beach USA Branksome Hall Asia International School Jeju-do Korea School of Arts & Sciences Emory University Atlanta USA School of Arts & Sciences Washington University St. Louis USA School of Arts & Sciences Johns Hopkins University Baltimore USA Fuzbien Technology Institute Rockville USA

Microbial fuel cell (MFC) is one of the clean and sustainable energy technologies, often referred to as renewable energy, and directly chemical energy contained in organic matter into electrical energy by using the catalytic activity of microorganisms. Cellulosic biomass is a particularly attractive renewable resource for its abundant supply at low cost and its neutral carbon balance. However, methanogenesis had been negatively linked to anaerobic cellulosic power generation in MFCs. Ginseng root is a saponin-rich plant material and red ginseng marc (RGM) has not been reused as a high-value resource for industry although its residue contained both electron donors and saponin, the potential power generation enhancers for MFC. In this study, RGM was supplemented into MFC to evaluate its effects on methanogenesis and power generation. Two-chamber H-type MFCs were established using rumen fluid as anolyte to ferment cellulose at 2% (w/v). RGM, the residue from the steam and press process for red ginseng beverage preparation, was freeze-dried and ground to pass 0.5 mm sieve and added to the anode of MFC at 1% (w/v;Exp. 1) or 0.1% (Exp. 2) dose for treatment. Open circuit voltage, voltage and current across an external resistor were measured daily for 10d. On d10 of operation, collected biogases were measured for total gas production and analyzed for its components. In Exp. 1, power density was between 44.0 and 97.2 with an average of 83.8 mW/m2 in 1% RGM MFCs and was between 45.2 and 76.3 with an average of 61.5 mW/m2 in control. In Exp. 2, power density was between 44.8 and 75.6 with an average of 60.9 mW/m2 in 0.1% RGM MFCs and was between 45.1 and 54.1 with an average of 49.7 mW/m2 in control. Total gas production for 10d was 563 and 523 mL for RGM and control, respectively, in Exp 1, and was 546 and 477 mL for RGM and control, respectively, in Exp 2. Methane took up 58.6 and 67.9% of total gas for RGM and control, respective

关键词： Microbial Fuel Cell Red Ginseng Marc Cellulose Methanogenesis Electricity

组织块重复贴壁使用在原代细胞培养中的实验意义

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宁夏医科大学学报 2012年第5期34卷 466-469,F0004页

作者：刘爽昝海英张鸣号姜怡邓曹军李桂忠宁夏医科大学基础医学院病理生理学系银川750004

目的旨在提高采用人脐静脉组织块贴壁法进行平滑肌细胞原代培养组织块的重复利用率,进而缩短采用细胞培养进行实验的培养周期。方法以改良人脐静脉平滑肌细胞贴壁培养法为基础,当原代培养细胞(对照组)进行第1次细胞传代后,将培养组织块... 详细信息

目的旨在提高采用人脐静脉组织块贴壁法进行平滑肌细胞原代培养组织块的重复利用率,进而缩短采用细胞培养进行实验的培养周期。方法以改良人脐静脉平滑肌细胞贴壁培养法为基础,当原代培养细胞(对照组)进行第1次细胞传代后,将培养组织块再次接种于新培养瓶中继续培养(实验组),分别记录两组细胞爬出时间、第1次传代时间、以及第1次传代后24、48、72、96h的细胞计数,通过倒置显微镜对两组细胞进行形态学观察,采用抗α-actin免疫细胞化学方法,对两组传代细胞的类型和性质进行鉴定。结果与对照组比较,实验组细胞爬出时间及第1次传代时间均明显缩短(P0.05);两组细胞形态经α-actin鉴定差异无统计学意义。结论人脐静脉组织块重复贴壁培养后,经传代其细胞数量、细胞形态与原代组织块贴壁培养的细胞差别均无统计学意义。

关键词：组织块重复贴壁原代培养平滑肌细胞

Measurement of the integrated luminosity of data samples collected during 2019-2022 by the Belle Ⅱ experiment

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Chinese Physics C 2025年第1期49卷 7-18页

作者： I.Adachi L.Aggarwal H.Ahmed J.K.Ahn H.Aihara N.Akopov A.Aloisio N.Althubiti N.Anh Ky D.M.Asner H.Atmacan T.Aushev V.Aushev M.Aversano R.Ayad V.Babu H.Bae S.Bahinipati P.Bambade Sw.Banerjee M.Barrett J.Baudot A.Baur A.Beaubien F.Becherer J.Becker J.V.Bennett F.U.Bernlochner V.Bertacchi M.Bertemes E.Bertholet M.Bessner S.Bettarini B.Bhuyan F.Bianchi L.Bierwirth T.Bilka D.Biswas A.Bobrov D.Bodrov J.Borah A.Boschetti A.Bozek P.Branchini T.E.Browder A.Budano S.Bussino Q.Campagna M.Campajola L.Cao G.Casarosa C.Cecchi J.Cerasoli M.-C.Chang P.Chang R.Cheaib P.Cheema B.G.Cheon K.Chilikin K.Chirapatpimol H.-E.Cho K.Cho S.-J.Cho S.-K.Choi S.Choudhury J.Cochran L.Corona J.X.Cui S.Das E.De La Cruz-Burelo S.A.De La Motte G.de Marino G.De Nardo G.De Pietro R.de Sangro M.Destefanis S.Dey R.Dhamija A.Di Canto F.Di Capua J.Dingfelder Z.Doležal I.Domínguez Jiménez T.V.Dong K.Dort D.Dossett S.Dubey K.Dugic G.Dujany P.Ecker D.Epifanov J.Eppelt P.Feichtinger T.Ferber T.Fillinger C.Finck G.Finocchiaro A.Fodor F.Forti A.Frey B.G.Fulsom A.gabrielli E.Ganiev M.Garcia-Hernandez R.Garg G.Gaudino V.Gaur A.Gaz A.Gellrich G.Ghevondyan D.Ghosh H.Ghumaryan G.Giakoustidis R.Giordano A.Giri P.Gironella B.Gobbo R.Godang O.Gogota P.Goldenzweig W.Gradl E.Graziani D.Greenwald Z.Gruberová T.Gu K.Gudkova I.Haide S.Halder Y.Han K.Hara T.Hara C.Harris K.Hayasaka H.Hayashii S.Hazra C.Hearty M.T.Hedges A.Heidelbach I.Heredia de la Cruz M.Hernández Villanueva T.Higuchi M.Hoek M.Hohmann R.Hoppe P.Horak C.-L.Hsu T.Humair T.Iijima K.Inami N.Ipsita A.Ishikawa R.Itoh M.Iwasaki W.W.Jacobs D.E.Jaffe E.-J.jang Q.P.Ji S.Jia Y.Jin A.Johnson K.K.Joo H.Junkerkalefeld M.Kaleta D.Kalita J.Kandra K.H.Kang G.Karyan T.Kawasaki F.Keil C.Kiesling C.-H.Kim D.Y.Kim J.-Y.Kim K.-H.Kim Y.-K.Kim Y.J.Kim H.Kindo K.Kinoshita P.Kodyš T.Koga S.Kohani K.Kojima A.Korobov S.Korpar E.Kovalenko R.Kowalewski P.Križan P.Krokovny T.Kuhr R.Kumar K.Kumara A.Kuzmin Y.-J.Kwon S.Lacaprara Y.-T.Lai K.Lalwani T.Lam L.Lanceri J.S.Lange M.Laurenza K.Lautenbach R.Leboucher M.J.Lee C.Lemettais P.Leo D.Lev 不详

series of data samples was collected with the Belle Ⅱ detector at the SuperKEKB collider from March 2019 to June *** determine the integrated luminosities of these data samples using three distinct methodologies involving Bhabha(e^(+)e^(-)→e^(+)e^(-)(ny),digamma(e^(+)e^(-)→γγ(nγ),and dimuon(e^(+)e^(-)→μ^(+)μ^(-)(nγ)*** total integrated luminosity obtained with Bhabha,digamma,and dimuon events is(426.88±0.03±2.61)fb^(-1),(429.28±0.03±2.62)fb^(-1),and(423.99±0.04±3.83)fb^(-1),where the first uncertainties are statistical and the second are *** resulting total integrated luminosity obtained from the combination of the three methods is(427.87±2.01)fb^(-1).

关键词： integrated luminosity Bhabha digamma dimuon BelleⅡ