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肌纤维类型对宰后成熟过程中驼肉品质的影响

西北农林科技大学学报（自然科学版） 2024年第10期52卷 1-10页

作者：吕浩迪李雅菲郑增拓乌音嘎明亮王琳琳 DULGUUN TUyATSETSEG Jambal 吉日木图内蒙古农业大学食品科学与工程学院内蒙古呼和浩特271018 蒙古国立科技大学蒙古乌兰巴托999097 中蒙生物高分子应用研究联合试验室蒙古乌兰巴托999097

【目的】研究不同部位驼肉的肌纤维组成及其对成熟过程中肉品质的影响,为驼肉的分部位精细加工提供理论支撑。【方法】收集内蒙古阿拉善4岁双峰驼的背最长肌(longissimus thoracis,LT)、半腱肌(semitendinosus,ST)和腰大肌(psoas major,... 详细信息

【目的】研究不同部位驼肉的肌纤维组成及其对成熟过程中肉品质的影响,为驼肉的分部位精细加工提供理论支撑。【方法】收集内蒙古阿拉善4岁双峰驼的背最长肌(longissimus thoracis,LT)、半腱肌(semitendinosus,ST)和腰大肌(psoas major,PM)肉样,利用ATPase酶染法和MyHC基因亚型PCR检测法,检测肌纤维组成类型、不同类型肌纤维数量和面积比及单个肌纤维的平均面积和直径;将3个部位驼肉样品在4℃下分别成熟1,6,12,24和72 h,检测不同时间驼肉的pH、色差(L、a^(*)、b^(*))、蒸煮损失和剪切力,分析肌纤维类型与肌纤维指标和肉品质指标的相关性。【结果】3个部位驼肉中均以Ⅱb型肌纤维占比最高,其中ST的Ⅰ型肌纤维数量比最低,仅为11.58%,显著低于LT和PM(Pb型肌纤维数量比为37.34%,显著低于LT和ST(Pb^(*)值随着成熟时间的延长呈上升趋势,a^(*)值随着成熟时间的延长呈先上升后下降趋势;PM的L、a^(*)、b^(*)值显著高于LT和ST(1 h L值除外)。蒸煮损失随着成熟时间的延长呈先下降后上升趋势,ST的蒸煮损失在72 h显著高于LT和PM(Pb^(*)值呈正相关;Ⅱb型肌纤维4个指标与pH呈负相关,与剪切力和蒸煮损失呈正相关;Ⅱx型肌纤维基因相对表达量与pH呈负相关,与剪切力呈正相关。【结论】Ⅰ型肌纤维占比高的肌肉,成熟过程中肉的色泽更鲜艳,嫩度更好;Ⅱx和Ⅱb型肌纤维占比高的肌肉,成熟过程中肉的剪切力大,嫩度差。

关键词：驼肉加工肌纤维类型宰后成熟驼肉品质

蒙药壮西-4调控miR-204对骨质疏松大鼠成骨细胞增殖的影响及机制

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中国老年学杂志 2024年第5期44卷 1124-1128页

作者：图布新通啦嘎包海金李桂花色·色日道尔吉瑟·道尔吉巴图浩仁他本蒙古国国立医科大学蒙古国乌兰巴托999097 内蒙古民族大学附属医院骨伤科内蒙古民族大学蒙医药学院

目的探讨蒙药壮西-4调控miR-204对骨质疏松大鼠成骨细胞增殖的影响及机制。方法将60只SD大鼠随机分为空白对照组、模型对照组、阳性对照组、低、中和高剂量组各10只。建模各组给予维A酸溶液灌胃建立骨质疏松模型,连续14 d。阳性对照... 详细信息

目的探讨蒙药壮西-4调控miR-204对骨质疏松大鼠成骨细胞增殖的影响及机制。方法将60只SD大鼠随机分为空白对照组、模型对照组、阳性对照组、低、中和高剂量组各10只。建模各组给予维A酸溶液灌胃建立骨质疏松模型,连续14 d。阳性对照组给予戊酸雌二醇灌胃,低、中、高剂量组分别给予不同剂量(50、100、200 mg/kg)蒙药壮西-4灌胃,空白对照组和模型对照组给予同体积的生理盐水灌胃,连续42 d。采用骨密度(bMD)仪测定大鼠第4腰椎及右股骨的bMD值,逆转录-聚合酶链反应(RT-PCR)血清miR-204表达量,酶联免疫吸附试验(ELISA)检测血清骨桥蛋白(OPG)、核因子κb受体活化因子配体(RANKL)含量。取MC3T3-E1细胞,分为对照组、促进组、抑制组和药物组,分别加入生理盐水、miR-204模拟物、miR-204抑制剂和蒙药壮西-4。采用RT-PCR法检测miR-204表达量,Western印迹检测RANKL、OPG蛋白表达量,噻唑蓝(MTT)检测细胞增殖情况,计算细胞存活率。结果各组第4腰椎和右股骨bMD值、血清miR-204表达量、血清OPG含量差异有统计学意义(均PbMD,其可能机制为通过上调miR-204表达促进大鼠成骨细胞增殖。

关键词：蒙药壮西-4 miR-204 骨质疏松成骨细胞增殖

Spatiotemporally resolved metabolomics and isotope tracing reveal CNS drug targets

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Acta Pharmaceutica Sinica b 2023年第4期13卷 1699-1710页

作者： bo Jin Xuechao Pang Qingce Zang Man ga Jing Xu Zhigang Luo Ruiping Zhang Jiangong Shi Jiuming He Zeper Abliz State Key Laboratory of bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China NMPA Key Laboratory for Safety Research and Evaluation of Innovative Drug Beijing 100050China Key Laboratory of Mass Spectrometry Imaging and Metabolomics(Minzu University of China) National Ethnic Affairs CommissionBeijing 100081China Key Laboratory of Ethnomedicine of Ministry of Education School of PharmacyMinzu University of ChinaBeijing 100081China

Deconvolution of potential drug targets of the central nervous system(CNS)is particularly challenging because of the complicated structure and function of the ***,a spatiotemporally resolved metabolomics and isotope tracing strategy was proposed and demonstrated to be powerful for deconvoluting and localizing potential targets of CNS drugs by using ambient mass spectrometry *** strategy can map various substances including exogenous drugs,isotopically labeled metabolites,and various types of endogenous metabolites in the brain tissue sections to illustrate their microregional distribution pattern in the brain and locate drug action-related metabolic nodes and *** strategy revealed that the sedative-hypnotic drug candidate yZG-331 was prominently distributed in the pineal gland and entered the thalamus and hypothalamus in relatively small amounts,and can increase glutamate decarboxylase activity to elevateγ-aminobutyric acid(gabA)levels in the hypothalamus,agonize organic cation transporter 3 to release extracellular histamine into peripheral *** findings emphasize the promising capability of spatiotemporally resolved metabolomics and isotope tracing to help elucidate the multiple targets and the mechanisms of action of CNS drugs.

关键词： Ambient mass spectrometry imaging Spatiotemporally resolved metabolomics Isotope tracing Drug targets Central nervous system Drug candidate

Laryngotracheal stenosis in burn patients requiring mechanical ventilation

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World Journal of Otorhinolaryngology-Head and Neck Surgery 2018年第2期4卷 117-121页

作者： yekaterina A. Koshkareva William b. hughes Ahmed M.S. Soliman Department of Otolaryngology — Head & Neck Surgery Lewis Katz School of Medicine at Temple University Philadelphia PA USA Temple burn Center Lewis Katz School of Medicine at Temple University Philadelphia PA USA

Objective: To identify the incidence of laryngotracheal stenosis (LTS) in burn pa-tients requiring mechanical ventilation at a regional academic burn center. Methods: A retrospective review of all burn patients requiring endotracheal intubation or tra-cheostomy for airway management between 2003 and 2009 was performed. A group of trauma patients requiring similar airway instrumentation during the same period of time was used as a control. Results: None of the trauma patients and 2 of the burn patients developed LTS. both presented with stridor and were diagnosed within 2—5 weeks after extubation. One patient underwent successful carbon dioxide laser radial incision and dilation and continues to do well. The other patient failed endoscopic treatment and required T-tube placement. The incidence of LTS in burn patients requiring mechanical ventilation was 2.98％ overall and 4.76％ among those with inhalational injury. Conclusions: Patients become symptomatic within weeks of the initial injury. Treatment is challenging and multiple surgical procedures are often required. A larger study is necessary to determine if the incidence is higher among burn patients.

关键词： Laryngeal stenosis Tracheal stenosis Subglottic stenosis Adult burn Inhalation

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盐酸普拉克索药剂学的研究进展

西北药学杂志 2024年第3期39卷 219-224页

作者：丁媛媛甘春雨嘎鲁马宇衡李瑞娟内蒙古医科大学药学院呼和浩特010110

目的了解盐酸普拉克索的药动学及药剂学方面的研究进展,为盐酸普拉克索新剂型的进一步开发提供参考以及新思路。方法通过查阅文献,对盐酸普拉克索的药动学及药剂学研究进行综述。结果盐酸普拉克索是选择性非麦角类多巴胺受体激动剂,一... 详细信息

目的了解盐酸普拉克索的药动学及药剂学方面的研究进展,为盐酸普拉克索新剂型的进一步开发提供参考以及新思路。方法通过查阅文献,对盐酸普拉克索的药动学及药剂学研究进行综述。结果盐酸普拉克索是选择性非麦角类多巴胺受体激动剂,一般用于帕金森病和不宁腿综合征的治疗,同时还具有多种药理作用,如抗抑郁作用、神经元保护作用、抗氧化作用和抗炎作用,受到医药界的广泛关注和重视。盐酸普拉克索具有口服吸收迅速、生物利用度高等特点。目前以盐酸普拉克索作为单一主药上市的制剂为口服途径给药的普通片剂与缓释片剂,但需多次服用药物,易出现血药浓度波动的现象,因此患者顺应性差,亟需得到改善。结论缓控迟释制剂、经皮给药制剂近年来发展迅速,逐步成为盐酸普拉克索制剂研发的重点。

关键词：盐酸普拉克索帕金森病药剂学药代动力学

Genetic Testing in Professional Football:Perspectives of Key Stakeholders

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Journal of Science in Sport and Exercise 2022年第1期4卷 49-59页

作者： Alexander b.T.McAuley David C.hughes Loukia G.Tsaprouni Ian Varley bruce Suraci homas R.Roos Adam J.Herbert Adam L.Kelly Faculty of Health Education and Life SciencesBirmingham City UniversityBirminghamWest MidlandsUK Department of Sport Science Nottingham Trent UniversityNottinghamUK Academy Coaching Department AFC BournemouthBournemouthUK The International Academy of Sports Science and Technology(AISTS) University of LausanneLausanneSwitzerland Department of Life Sciences Birmingham City UniversityCity South CampusWestbourne RoadEdgbastonBirmingham B153TNUK

Purpose Genetic research in football is currently in it’s infancy but is growing ***,the practical application of genetic testing in football and the views concerning its use are ***,the purpose of this study was to assess the current practical application of genetic testing in professional football and provide an insight into the perspectives of key stakeholders(i.e.,coaches,practitioners,players).Methods In total,122 participants completed an online anonymous *** consisted of 21 multiple choice and Likert scale questions,with the option of providing an explanation for each *** Findings revealed genetic testing is rarely utilised by key stakeholders(10%)or their respective organisations(14%).However,three quarters(75%)had the opinion that genetic testing will have great utility in the *** majority(72%)believed genetic testing should be used for athlete development and injury risk,whilst 35%believed that genetic testing should be utilised for talent identification ***,most key stakeholders viewed their own(89%)and their col-leagues’(79%)knowledge related to genetic testing as insufficient;mainly due to ineffective current communication methods(91%).Most believed educational workshops are required(71%),whilst nearly all(91%)were interested in developing their expertise on the utility of genetic *** Genetic testing is rarely used within professional football,although key stakeholders anticipate that it will be utilised more in the *** such,educational support may prove valuable in improving key stakeholder knowledge and the practical application of genetic testing in professional football.

关键词： Soccer Talent identification Athlete development Genetics

LASER LIFT-OFF OF gaN THIN FILMS FROM SAPPHIRE SUbSTRATES

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Acta Metallurgica Sinica(English Letters) 2001年第6期14卷 448-452页

作者： J. Xu, R. Zhang, y.P. Wang, X.Q. Xiu, S.L. Gu, b. Shen, y. Shi, Z.G. Liu and y.D. Zheng (Department of Physics and National Laboratory of Solid State Microstructures, Nanjing University, Nanjing 210093, China) Dept. of Phys. Nanjing Univ. Nanjing 210093 China

gallium Nitride film was successfully separated from sapphire substrate by laser radiation. The absorption of the 248 nm radiation by the gaN at the interface results in rapid thermal decomposition of interfacial layer, yielding metallic ga and N2 gas. The substrate can be easily removed by heating above the ga melting point (29°C). X-ray diffraction, atomic force microscopy and photoluminescence of gaN before and after lift-off process have been performed, which demonstrated that the separation and transfer process do not alter the structural quality of the gaN films. And further discussions on the threshold energy and crack-free strategies of laser lift-off process have also been presented.

关键词： gallium compounds Laser beam effects Nitrides Sapphire Separation

Promotion of the articular cartilage proteoglycan degradation by T-2 toxin and selenium protective effect

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Journal of Zhejiang University-Science b(biomedicine & biotechnology) 2008年第1期9卷 22-33页

作者： Si-yuan LI Jun-ling CAO Zhong-li SHI Jing-hong CHEN Zeng-tie ZHANG Clare E. hughes bruce CATERSON Institute of Endemic Diseases College of Medicine Xi＇an Jiaotong University Key Laboratory of Environment and Genes Related to Diseases （Xi＇an Jiaotong University） Ministry of Education Kev Laboratory of Microelement and Endemic Disease （Xi＇an Jiaotong University） Ministr） of Health Xi＇an 710061 China Laboratory of Connective Tissue biology School of Biosciences Cardiff University Cardiff CF10 3US UK

Objective: To identify the relationship between T-2 toxin and Kashin-beck disease (KbD),the effects of T-2 toxin on aggrecan metabolism in human chondrocytes and cartilage were investigated in vitro. Methods: Chondrocytes were isolated from human articular cartilage and cultured in vitro. Hyaluronic acid (HA),soluble CD44 (sCD44),IL-1β and TNF-α levels in super-natants were measured by enzyme-linked immunosorbent assay (ELISA). CD44 content in chondrocyte membrane was deter-mined by flow cytometry (FCM). CD44,hyaluronic acid synthetase-2 (HAS-2) and aggrecanases mRNA levels in chondrocytes were determined using reverse transcription polymerase chain reaction (RT-PCR). Immunocytochemical method was used to investigate expressions of bC-13,3-b-3(-) and 2-b-6 epitopes in the cartilage reconstructed in vitro. Results: T-2 toxin inhibited CD44,HAS-2,and aggrecan mRNA expressions,but promoted aggrecanase-2 mRNA expression. Meanwhile,CD44 expression was found to be the lowest in the chondrocytes cultured with T-2 toxin and the highest in control plus selenium group. In addition,ELISA results indicated that there were higher sCD44,IL-1β and TNF-α levels in T-2 toxin group. Similarly,higher HA levels were also observed in T-2 toxin group using radioimmunoprecipitation assay (RIPA). Furthermore,using monoclonal antibodies bC-13,3-b-3 and 2-b-6,strong positive immunostaining was found in the reconstructed cartilage cultured with T-2 toxin,whereas no positive staining or very weak staining was observed in the cartilage cultured without T-2 toxin. Selenium could partly inhibit the effects of T-2 toxin above. Conclusion: T-2 toxin could inhibit aggrecan synthesis,promote aggrecanases and pro-inflammatory cytokines production,and consequently induce aggrecan degradation in chondrocytes. These will perturb metabolism balance between aggrecan synthesis and degradation in cartilage,inducing aggrecan loss in the end,which may be the initiation of the cartilage degradation.

关键词： T-2 toxin Kashin-beck disease (KbD) Aggrecan IL-1β TNF-α Aggrecanase Hyaluronic acid (HA) CD44

Increased expression of chondroitin sulphate proteoglycans in rat hepatocellular carcinoma tissues

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World Journal of gastroenterology 2012年第30期18卷 3962-3976页

作者： Xiao-Li Jia Si-yuan Li Shuang-Suo Dang yan-An Cheng Xin Zhang Wen-Jun Wang Clare E hughes bruce Caterson Department of Infectious Diseases The Second Affiliated Hospital of Medical School of Xi'an Jiaotong University Xi'an 710004 Shaanxi Province China Connective Tissue biology Laboratories Division of Pathophysiology and Repair School of Biosciences Cardiff University Cardiff Wales CF10 3AX United Kingdom The Institute of Endemic Disease Medical School of Xi'an Jiaotong University Xi'an 710061 Shaanxi Province China

AIM:To investigate the expression of chondroitin sulphate proteoglycans(CSPGs)in rat liver tissues of hepatocellular carcinoma(HCC).METHODS:Thirty male Sprague Dawley rats were randomly divided into two groups:control group(n=10) and HCC model group(n=20).Rats in the HCC model groups were intragastrically administrated with 0.2%(w/v)N-diethylnitrosamine(DEN)every 5 d for 16 wk,whereas 0.9%(w/v)normal saline was administered to rats in the control *** 16 wk from the initiation of experiment,all rats were killed and livers were collected and fixed in 4%(w/v)*** tissues were embedded in paraffin and *** staining(hematoxylin and eosin and Toluidine blue)was performed to demonstrate the onset of HCC and the content of sulphated glycosaminoglycan(sgaG).Immunohistochemical staining was performed to investigate the expression of chondroitin sulphate(CS)/dermatan sulphate(DS)-gaG,heparan sulphate(HS)-gaG,keratan sulphate(KS)-gaG in liver ***,expression and distribution of CSPG family members,including aggrecan,versican,biglycan and decorin in liver tissues,were also immunohistochemically ***:After 16 wk administration of DEN,malignant nodules were observed on the surface of livers from the HCC model group,and their hepatic lobule structures appeared largely disrupted under *** blue staining demonstrated that there was an significant increase in sgaG content in HCC tissues when compared with that in the normal liver tissues from the control group[0.37±0.05 integrated optical density per stained area(IOD/area)and 0.21± 0.01 IOD/area,PgaG in HCC tissues was induced by an elevated expression of CS/DS(0.28±0.02 IOD/area and 0.18±0.02 IOD/area,Pbut not KS gaGs in HCC *** studies thereby were performed to investigate the expression and distribution o

关键词： Hepatocellular carcinoma Proteoglycan Chondroitin sulphate Heparan sulphate Keratan sulphate