检索结果-南通市图书馆

Horticultural Plant Journal 2020年第4期6卷 247-256页

作者： Ming Zhou shaogui guo Shouwei Tian Jie Zhang Yi Ren guoyi Gong Changbao Li Haiying Zhang Yong Xu Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China) Beijing Key Laboratory of Vegetable Germplasm ImprovementNational Engineering Research Center for VegetablesBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China

Watermelon fruit undergoes distinct development stages with dramatic changes during fruit *** date,the molecular mechanics of watermelon ripening remain *** and transcriptome evidences suggested that the ethylene response factor(ERF)gene ClERF069 may be an important candidate factor affecting watermelon fruit *** dissect the roles of ClERF069 in fruit ripening,structure and phylogenetic analysis were performed using the amplified full-length ***-ripening watermelon 97103,non-ripening watermelon PI296341-FR and the RIL population were used to analyze ClERF069 expression dynamics and the correlation with fruit ripening *** results indicated that ClERF069 belongs to ERF family group VI and show high homology(83%identity)to melon ERF069-like ***069 expression in watermelon flesh was negatively correlated with fruit lycopene content and sugar content during fruit ripening *** transgenic evidences indicated that overexpression of 35S:ClERF069 in tomato noticeably delayed the ripening process up to 5.2 ***,β-carotenoid accumulation patterns were altered and ethylene production patterns in transgenic fruits was significantly delayed during fruit *** together,watermelon ethylene response factor ClERF069 was concluded to be a negative regulator of fruit ripening.

关键词： watermelon ethylene response factor ERF transgenic tomato ripening

Natural variation in the NAC transcription factor NONRIPENING contributes to melon fruit ripening

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2022年第7期64卷 1448-1461页

作者： Jinfang Wang Shouwei Tian Yongtao Yu Yi Ren shaogui guo Jie Zhang Maoying Li Haiying Zhang guoyi Gong Min Wang Yong Xu National Watermelon and Melon Improvement Center Beijing Academy of Agricultural and Forestry SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)Beijing Key Laboratory of Vegetable Germplasm ImprovementBeijing 100097China Sanya Institute Hainan Academy of Agricultural SciencesHaikou 572025China

ThThe NAC transcription factor NONRIPENING(NOR)is a master regulator of climacteric fruit ***(Cucumis melo L.)has climacteric and nonclimacteric fruit ripening varieties and is an ideal model to study fruit *** natural CmNAC-NOR variants,the climacteric haplotype CmNAC-NOR^(S,N) and the non-climacteric haplotype CmNAC-NOR^(A,S),have effects on fruit ripening;however,their regulatory mechanisms have not been ***,we report that a natural mutation in the transcriptional activation domain of CmNAC-NORS,Ncontributes to climacteric melon fruit ***-NOR knockout in the climacteric-type melon cultivar“BYJH”completely inhibited fruit ripening,while ripening was delayed by 5-8 d in heterozygous cmnac-nor mutant *** AC-NOR directly activated carotenoid,ethylene,and abscisic acid biosynthetic genes to promote fruit coloration and ***,CmNAC-NOR mediated the transcription of the“CmNAC-NOR-CmNAC73-CmCWINV2”module to enhance flesh *** transcriptional activation activity of the climacteric haplotype CmNAC-NORS,Non these target genes was significantly higher than that of the nonclimacteric haplotype CmNAC-NOR^(A,S).Moreover,CmNAC-NORS,Ncomplementation fully rescued the non-ripening phenotype of the tomato(Solanum lycopersicum)cr-nor mutant,while CmNAC-NOR^(A,S) did *** results provide insight into the molecular mechanism of climacteric and non-climacteric fruit ripening in melon.

关键词： climacteric fruit fruit ripening melon NAC tran-scription factor

ClSnRK2.3 negatively regulates watermelon fruit ripening and sugar accumulation

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2023年第10期65卷 2336-2348页

作者： Jinfang Wang Yanping Wang Yongtao Yu Jie Zhang Yi Ren Shouwei Tian Maoying Li Shengjin Liao shaogui guo guoyi Gong Haiying Zhang Yong Xu State Key Laboratory of Vegetable Biobreeding National Engineering Research Center for VegetablesBeijing Key Laboratory of Vegetable Germplasms ImprovementBeijing Vegetable Research CenterBeijing Academy of Agriculture and Forestry ScienceBeijing 100097China

Watermelon(Citrullus lanatus) as non-climacteric fruit is domesticated from the ancestors with inedible fruits. We previously revealed that the abscisic acid(ABA) signaling pathway gene ClSnRK2.3 might infuence watermelon fruit ripening. However,the molecular mechanisms are unclear. Here,we found that the selective variation of ClSnRK2.3 resulted in lower promoter activity and gene expression level in cultivated watermelons than ancestors, which indicated ClSnRK2.3 might be a negative regulator in fruit ripening. Overexpression (OE) of ClSnRK2.3 significantly delayed watermelon fruit ripening and suppressed the accumulation of sucrose, ABA and gibberellin GA4. Furthermore,we determined that the pyrophosphate-dependent phosphofructokinase(ClPFP1) in sugar metabolism pathway and GA biosynthesis enzyme GA20 oxidase(Cl GA20ox) could be phosphorylated by ClSnRK2.3 and thereby resulting in accelerated protein degradation in OE lines and finally led to low levels of sucrose and GA4. Besides that, ClSnRK2.3 phosphorylated homeodomain-leucine zipper protein(ClHAT1) and protected it from degradation to suppress the expression of the ABA biosynthesis gene 9’-cis-epoxycarotenoid dioxygenase 3(Cl NCED3). These results indicated that ClSnRK2.3 negatively regulated watermelon fruit ripening by manipulating the biosynthesis of sucrose, ABA and GA4. Altogether, these findings revealed a novel regulatory mechanism in non-climacteric fruit development and ripening.

关键词： fruit ripening SnRK2.3 sucrose watermelon

Modulation of Sex Expression in Four Forms of Watermelon by Gibberellin, Ethephone and Silver Nitrate

在线全文

学校读者我要写书评

暂无评论

Horticultural Plant Journal 2017年第3期3卷 91-100页

作者： ZHANG Jie SHI Jianting JI Gaojie ZHANG Haiying GONG guoyi guo shaogui REN Yi FAN Jianguang TIAN Shouwei XU Yong National Engineering Research Center for Vegetables Beijing Academy of Agriculture and Forestry SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)Beijing Key Laboratory of Vegetable Germplasm Improvement

There has been no systematic research on the effect of plant growth regulators and silver nitrate treatments on the control of sex expression in watermelon. In this study, we tested sex expression responses of four watermelon forms(monoecism, gynoecism, andromonoecism,and hermaphrodite) to gibberellin, ethephon and silver nitrate treatments. Results have shown that, in monoecious plants, gibberellins(GA_3)and ethephon treatments reduced the percentage of female flowers and delayed the occurrence of the first female flower, while silver nitrate induced the formation of bisexual flowers. In gynoecious plants, both ethephon and silver nitrate treatments transformed some female flowers into bisexual flowers, and treatment with ethephon resulted in a mass of abnormal flowers, while no obvious effect of treatment with GA_3 was observed. In andromonoecious plants, ethephon and GA_3 treatments delayed the occurrence of the first bisexual flower, and GA_3 reduced the percentage of bisexual flowers, while no distinct effect for silver nitrate treatment was observed. In hermaphroditic plants, ethephon treatment induced the appearance of numerous abnormal flowers, while no obvious effects for GA_3 and silver nitrate treatments were *** analyzed the transcription levels of all the expressed aminocyclopropane-1-carboxylic acid synthase(ACS) homologues in two gynoecious mutants and their wild types. We also tested the gene expression of CitACS4 which had been recognized as the andromonoecious gene in all treatments. All these results suggested that the best masculinizing treatment for breeding of the gynoecious line is silver nitrate, which repressed the expression of CitACS4 and induced many bisexual flowers for use in self-fertilization subsequently.

关键词： watermelon sex expression gibberellins(GA3) ethephon silver nitrate

Mutation in the gene encoding 1-aminocyclopropane-1-carboxylate synthase 4 (CitACS4) led to andromonoecy in watermelon

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2016年第9期58卷 762-765页

作者： Gaojie Ji Jie Zhang Haiying Zhang Honghe Sun guoyi Gong Jianting Shi Shouwei Tian shaogui guo Yi Ren Huolin Shen Junping Gao Yong Xu Beijing Vegetable Research Center National Engineering Research Center for Vegetables Beijing Academy of Agriculture and Forestry Sciences Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)Ministry of Agriculture Beijing Key Laboratory of Vegetable Germplasm ImprovementBeijing 100097China Department of Vegetable Science College of Horticulture China Agricultural University Beijing 100097 China

Summary Although it has been reported previously that ethylene plays a critical role in sex determination in cucurbit species, how the andromonoecy that carries both the male and hermaphroditic flowers is determined in watermelon is still unknown. Here we showed that the watermelon gene 1-aminocyclopropane-1-carboxylate syn- thase 4 CCitACS4）, expressed specifically in carpel primor- dia, determines the andromonoecy in watermelon. Among four single nucleotide polymorphism （SNPs） and one lnDel identified in the coding region of CitACS4, the C364W mutation located in the conserved box 6 was co- segregated with andromonoecy. Enzymatic analyses showed that the C364W mutation caused a reduced activity in CitACS4. We believe that the reduced CitACS4 activity may hamper the programmed cell death in stamen primordia, leading to the formation of hermaphroditic flowers.

关键词： 1-aminocyclopropane-1-carboxylic acid synthase Citrullus lanatus sex determination

在线全文

学校读者我要写书评

暂无评论

苏北盆地天然气成藏特征及选区评价

天然气工业 1999年第3期19卷 6-14页

作者：郭念发郑绍贵中国新星石油公司华东石油地质研究院

苏北盆地面积３．８×１０４ｋｍ２，天然气资源丰富，发育了海相下、中、上古生界三套巨厚烃源岩系和陆相早第三系及局部地区中、下侏罗统两套富含有机质的暗色泥岩。海相地层中由于裂隙、晶间孔、溶孔、溶缝及晶洞发育，弥补了原生孔... 详细信息

苏北盆地面积３．８×１０４ｋｍ２，天然气资源丰富，发育了海相下、中、上古生界三套巨厚烃源岩系和陆相早第三系及局部地区中、下侏罗统两套富含有机质的暗色泥岩。海相地层中由于裂隙、晶间孔、溶孔、溶缝及晶洞发育，弥补了原生孔隙储集能力的不足，使其变为有效的储集岩。陆相早第三系储层平均孔隙度大于２５％，渗透率大于１×１０－３μｍ２。盆地内纵向上发育四套区域盖层，自上而下为上第三系盐城组厚层泥岩、白垩系浦口组泥岩与膏盐层、二叠系龙潭组泥岩与煤层系、志留系高家边组泥岩，这些盖层分别封隔了新生界、中生界、上古生界及下古生界储层中的天然气，配置成四套有利的储盖组合。该盆地天然气类型主要有三种：生物气———低温热解气，如刘庄气田；油田伴生气———凝析气，如周庄、永安气田；多源混合气（含幔源气），如黄桥气田。该盆地成藏控制因素有四：多气源、高生气强度；有利构造带；晚期断裂活动；地质背景不同，圈闭类型不同。综上所述提出苏北盆地今后天然气勘探的方向有三：小海—海安区块、淮阴区块和泰兴—如皋区块。

关键词：苏北盆地天然气气藏形成勘探区成藏特征

Karyotype Stability and Unbiased Fractionation in the Paleo-Allotetraploid Cucurbita Genomes

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2017年第10期10卷 1293-1306页

作者： Honghe Sun Shan WU guoyu Zhang Chen Jiao shaogui guo Yi Ren Jie Zhang Haiying Zhang guoyi Gong Zhangcai Jia Fan Zhang Jiaxing Tian William J. Lucas Jeff J. Doyle Haizhen Li Zhangjun Fei Yong Xu National Engineering Research Center for Vegetables Beijing Academy of Agriculture and Forestry Sciences Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China) Beijing Key Laboratory of Vegetable Germplasm Improvement Beijing 100097 China Boyce Thompson Institute Cornell University Ithaca NY 14853 USA Department of Plant Biology College of Biological Sciences University of California Davis CA 95616 USA Section of Plant Breeding & Genetics School of Integrated Plant Sciences Cornell University Ithaca NY 14853 USA USDA-ARS Robert W. Holley Center for Agriculture and Health Ithaca NY 14853 USA

The Cucurbita genus contains several economically important species in the Cucurbitaceae family. Here, we report high-quality genome sequences of C. maxima and C. moschata and provide evidence supporting an allotetraploidization event in Cucurbita. We are able to partition the genome into two homoeologous subgenomes based on different genetic distances to melon, cucumber, and watermelon in the Benincaseae tribe. We estimate that the two diploid progenitors successively diverged from Benincaseae around 31 and 26 million years ago （Mya）, respectively, and the allotetraploidization happened at some point between 26 Mya and 3 Mya, the estimated date when C. maxima and C. moschata diverged. The subgenomes have largely maintained the chromosome structures of their diploid progenitors. Such long-term karyotype stability after polyploidization has not been commonly observed in plant polyploids. The two subgenomes have retained similar numbers of genes, and neither subgenome is globally dominant in gene expression. Allele-specific expression analysis in the C. maxima ×C. moschata interspecific F1 hybrid and their two parents indicates the predominance of trans-regulatory effects underlying expression divergence of the parents, and detects transgressive gene expression changes in the hybrid correlated with heterosis in important agronomic traits. Our study provides insights into polyploid genome evolution and valuable resources for genetic improvement of cucurbit crops.

关键词： Cucurbita maxima Cucurbita moschata paleo-allotetraploid karyotype stability unbiased fractionation allele-specific expression

CRISPR/Cas9-mediated mutagenesis of CiBG1 decreased seed size and promoted seed germination in watermelon

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2021年第1期8卷 1013-1024页

作者： Yanping Wang Jinfang Wang shaogui guo Shouwei Tian Jie Zhang Yi Ren Maoying Li guoyi Gong Haiying Zhang Yong Xu National Watermelon and Melon Improvement Center Beijing Academy of Agricultural and Forestry SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)Beijing Key Laboratory of Vegetable Germplasm ImprovementBeijing 100097China

Abscisic acid(ABA)is a critical regulator of seed development and germination.β-glucosidases(BGs)have been suggested to be contributors to increased ABA content because they catalyze the hydrolysis of ABA-glucose ester to release free ***,whether BGs are involved in seed development is *** this study,a candidate gene,CiBG1,in watermelon was selected for targeted mutagenesis via the CRISPR/Cas9 *** size and weight were significantly reduced in the Clbg1-mutant watermelon lines,which was mainly attributed to decreased cell number resulting from decreased ABA levels.A transcriptome analysis showed that the expression of 1015 and 1429 unique genes was changed 10 and 18 days after pollination(DAP),***-and cell cycle-related genes were enriched in the differentially expressed genes of wild type and Clbg1-mutant lines during seed ***,the expression of genes in the major signaling pathways of seed size control was also *** addition,seed germination was promoted in the Cibg1-mutant lines due to decreased ABA *** results indicate that ClBG1 may be critical for watermelon seed size regulation and germination mainly through the modulation of ABA content and thereby the transcriptional regulation of cytoskeleton-,cell cycle-and signaling-related *** results lay a foundation for dissecting the molecular mechanisms of controlling watermelon seed size,a key agricultural trait of significant economic importance.

关键词： CRISPR/Cas9 size thereby

Spd和MGBG对黄瓜子房内源多胺和蛋白质组成的影响及与单性结实的关系

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

园艺学报 2005年第4期32卷 632-637页

作者：陈学好于杰徐强郭绍贵扬州大学农学院园艺系扬州225009

以黄瓜(Cucum is sativusL.)单性结实品系EP-6与非单性结实品系ZR-2为试材,研究外源亚精胺(Spd)和多胺合成抑制剂MGBG处理对子房(幼果)内源多胺含量和蛋白质组成的影响及其与黄瓜单性结实和幼果发育的关系。结果表明:花前2 d对ZR-2不授... 详细信息

以黄瓜(Cucum is sativusL.)单性结实品系EP-6与非单性结实品系ZR-2为试材,研究外源亚精胺(Spd)和多胺合成抑制剂MGBG处理对子房(幼果)内源多胺含量和蛋白质组成的影响及其与黄瓜单性结实和幼果发育的关系。结果表明:花前2 d对ZR-2不授粉子房施用外源亚精胺能显著提高子房内源亚精胺、精胺与腐胺水平,促进其单性结实和果实生长,而花前2 d对EP-6不授粉子房外源施用MGBG则显著降低其子房内源亚精胺和精胺水平,提高了内源腐胺水平,导致子房萎蔫。运用SDS-PAGE法研究了外源亚精胺和MGBG处理后子房中可溶性蛋白质组成的变化,结果表明:ZR-2用外源Spd处理,开花当天至花后3 d的子房中出现了分子量为55 kD与37 kD的两种特异蛋白质,花后1 d至花后3 d又出现了88 kD与82 kD两种特异蛋白质;单性结实品系EP-6用MGBG处理子房,开花当天至花后3 d出现了47 kD与25kD两种特异蛋白质,引起85 kD、45 kD与10 kD蛋白质的消失。这些结果表明,外源多胺和多胺合成抑制剂处理可能是通过改变内源多胺水平和蛋白质合成而影响单性结实和子房(幼果)发育。

关键词：黄瓜多胺特异蛋白质单性结实

在线全文

学校读者我要写书评

暂无评论

早熟优质耐裂西瓜新品种京欣四号的选育

长江蔬菜 2008年第6X期 22-24页

作者：宫国义许勇张海英郭绍贵国家蔬菜工程技术研究中心北京100097

西瓜新品种京欣四号早熟,果实发育期28 d,全生育期90d左右。植株生长势强,抗病,坐瓜性好。果实圆形,绿底覆盖墨绿窄条纹,外形美观。单瓜质量7～8 kg,剖面均匀,红瓤,中心可溶性固形物含量11.5%。皮薄,耐裂,耐储运,肉质脆嫩,口感佳。与京... 详细信息

西瓜新品种京欣四号早熟,果实发育期28 d,全生育期90d左右。植株生长势强,抗病,坐瓜性好。果实圆形,绿底覆盖墨绿窄条纹,外形美观。单瓜质量7～8 kg,剖面均匀,红瓤,中心可溶性固形物含量11.5%。皮薄,耐裂,耐储运,肉质脆嫩,口感佳。与京欣一号相比,耐裂性有较大提高,中心糖度高,瓤色更红。适于早春小拱棚、露地和秋大棚栽培及远距离运输。

关键词：京欣四号优质耐裂品种选育