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Thermodynamics and Magnetostriction of Fe-Co-B-Si-Nb-C Bulk Metallic Glasses

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Journal of Iron and Steel Research International 2013年第2期20卷 58-61页

作者： GAO Zhen NI Xiao-jun guo jin-hua LI De-ren LU Zhi-chao ZHOU Shao-xiong Advanced Technology and Materials Co Ltd China Iron and Steel Research Institute Group

Fe-Co-B-Si-Nb-C glassy alloy with the addition of C was prepared by arc melting and copper suck-casting. The thermodynamics and soft magnetic properties were investigated. The casted amorphous alloys were heat-treated at different temperatures. The differential scanning calorimeter （DSC） results and thermal expansion show complete thermodynamics of crystallization. Its magnetostriction properties were studied by capacitance method. Saturation magnetostriction increases to 38×10^-6. The amorphous alloy exhibits good soft magnetic properties with low coercivity and high saturation magnetic induction. The results show that minor addition of C is beneficial to enhance the saturation magnetostriction λs, and do not deteriorate the ability of forming amorphous.

关键词： amorphous material soft magnetic material magnetostriction thermal expansion

Applications of gastric peroral endoscopic myotomy in the treatment of upper gastrointestinal tract disease

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World Journal of Gastrointestinal Surgery 2024年第3期16卷 658-669页

作者： Shi-Yu Chang guo-hua jin Hai-Bo Sun Dong Yang Tong-Yu Tang Department of Gastroenterology The First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina

Gastric peroral endoscopic myotomy(G-POME)is an emerging minimally invasive endoscopic technique involving the establishment of a submucosal tun-nel around the pyloric *** 2013,Khashab et al used G-POME for the first time in the treatment of gastroparesis with enhanced therapeutic efficacy,prov-iding a new direction for the treatment of *** the recent and rapid development of G-POME therapy technology,progress has been made in the treatment of gastroparesis and other upper digestive tract diseases,such as congenital hypertrophic pyloric stenosis and gastric sleeve stricture,with *** article reviews the research progress and future prospects of G-POME for the treatment of upper digestive tract gastrointestinal diseases.

关键词： Gastric peroral endoscopic myotomy Upper digestive tract diseases Gastroparesis Congenital hypertrophic pyloric stenosis Gastric sleeve stricture

miR-103-3p targets Ndel1 to regulate neural stem cell proliferation and differentiation

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Neural Regeneration Research 2022年第2期17卷 401-408页

作者： Wen Li Shan-Shan Wang Bo-Quan Shan Jian-Bing Qin He-Yan Zhao Mei-Ling Tian Hui He Xiang Cheng Xin-hua Zhang guo-hua jin Department of Human Anatomy Institute of NeurobiologyNantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education NantongJiangsu ProvinceChina Co-Innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina

The regulation of adult neural stem cells(NSCs) is critical for lifelong neurogenesis. MicroRNAs(miRNAs) are a type of small, endogenous RNAs that regulate gene expression post-transcriptionally and influence signaling networks responsible for several cellular processes. In this study, mi R-103-3 p was transfected into neural stem cells derived from embryonic hippocampal neural stem cells. The results showed that mi R-103-3 p suppressed neural stem cell proliferation and differentiation, and promoted apoptosis. In addition, mi R-103-3 p negatively regulated Nud E neurodevelopment protein 1-like 1(Ndel1) expression by binding to the 3′ untranslated region of Ndel1. Transduction of neural stem cells with a lentiviral vector overexpressing Ndel1 significantly increased cell proliferation and differentiation, decreased neural stem cell apoptosis, and decreased protein expression levels of Wnt3 a, β-catenin, phosphor-GSK-3β, LEF1, c-myc, c-Jun, and cyclin D1, all members of the Wnt/β-catenin signaling pathway. These findings suggest that Ndel1 is a novel mi R-103-3 p target and that mi R-103-3 p acts by suppressing neural stem cell proliferation and promoting apoptosis and differentiation. This study was approved by the Animal Ethics Committee of Nantong University, China(approval No. 20200826-003) on August 26, 2020.

关键词： apoptosis canonical Wnt pathway differentiation MiR-103-3p Ndel1 neural stem cells neurogenesis proliferation

Improved Isolation and Culture of Embryonic Germ Cells from Guanzhong Dairy Goat

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Agricultural Sciences in China 2006年第7期5卷 550-557页

作者： YANG Wei-feng GE Xiu-guo hua jin-lian SHEN Wen-zheng DOU Zhong-ying Shaanxi Center of Stem Cells Engineering ＆ Technology Northwest A ＆ F University Yangling 712100 P.R.China

A total of 219 embryonic-germ-cell-like （EG-like） clumps were derived from 15 selected goat fetuses. Isolation of primordial germ cells （PGCs） based on co-culture with primary goat embryonic fibroblast showed no difference from traditional feeder layer-based culture method used in mouse and human. The putative primary EG colonies were multilayer clumps of compact cells with unclear cell-cell boundaries. Three subculture methods of goat EG-like colony, traditional enzymatic digestion, mechanical cutting and combination of the both, were compared in this study. As a result, EG-like colonies traditionally disassociated with collagenase 1V could be subcultured for up to 4 passages. And the mechanically disaggregated EG-like colonies were successfully maintained 9-12 passages with or without enzymatic treatment. The pluripotency of the EG-like colonies was identified by their specific marker staining, spontaneous differentiation and embryoid bodies （EBs） formation in vitro. Most goat EG-like colonies （〉 80%） were AKP positive and immunocytochemically characterized with positive SSEA-1, Oct-4 and c-kit staining but SSEA-4. Under the condition of delaying passage, goat EG-like cells could differentiate into fibroblast-like, epithelium-like, and neuron-like cells. In addition, EBs could be obtained successfully in routine hanging drop culture. The serum free culture system （feeder layer-based） used in this study was suitable for keeping PGCs and EG-like cells in their undifferentiated condition, but failed to converse them to immortal cells. These results indicated that mechanical cutting is an effective method for passaging goat EG cell colonies. However, the microenvironment of conversing EG cells to immortal cells is still unclear.

关键词： goat primordial germ cells embryonic germ cells co-culture mechanical cutting

Neural differentiation of human Wharton＇s jelly-derived mesenchymal stem cells improves the recovery of neurological function after transplantation in ischemic stroke rats

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Neural Regeneration Research 2017年第7期12卷 1103-1110页

作者： Lei Zhang Lin-mei Wang Wei-wei Chen Zhi Ma Xiao Han Cheng-ming Liu Xiang Cheng Wei Shi jing-jing guo Jian-bing Qin Xiao-qing Yang guo-hua jin Xin-hua Zhang Department of Anatomy Nantong University Nantong Jiangsu Province China Department of Radiation Oncology Third People's Hospital of Yancheng Yancheng Jiangsu Province China Department of Neurosurgery the-Affiliated Hosptial of Nantong University Nantong Jiangsu Province China Department of Obstetricsand Gynecology the Affiliated Hosptial of Nantong University Nantong Jiangsu Province China Co-innovation Center of Neuroregeneration Nantong University Nantong Jiangsu Province China

Human Wharton＇s jelly-derived mesenchymal stem cells（h WJ-MSCs）have excellent proliferative ability,differentiation ability,low immunogenicity,and can be easily ***,there are few studies on their application in the treatment of ischemic stroke,therefore their therapeutic effect requires further *** this study,h WJ-MSCs were transplanted into an ischemic stroke rat model via the tail vein 48 hours after transient middle cerebral artery *** 4 weeks,neurological functions of the rats implanted with h WJ-MSCs were significantly ***,many h WJ-MSCs homed to the ischemic frontal cortex whereby they differentiated into neuron-like cells at this *** results confirm that h WJ-MSCs transplanted into the ischemic stroke rat can differentiate into neuron-like cells to improve rat neurological function and behavior.

关键词： nerve regeneration human Wharton's jelly-derived mesenchymal stem cells ischemic stroke cell transplantation middle cerebral arteryocclusion neural differentiation neurological function neural regeneration

Long non-coding RNA GAS5 promotes PC12 cells differentiation into Tuj1-positive neuron-like cells and induces cell cycle arrest

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Neural Regeneration Research 2019年第12期14卷 2118-2125页

作者： He-Yan Zhao Sheng-Tong Zhang Xiang Cheng Hao-Ming Li Lei Zhang Hui He Jian-Bing Qin Wei-Ye Zhang Yan Sun guo-hua jin Department of Human Anatomy the Jiangsu Key Laboratory of Neuroregeneration Medical School Nantong University

Growth arrest-specific 5 (GAS5) is an anti-oncogene that has been extensively studied in tumors. However, research on GAS5 in the context of nervous system disease is rare at present. This study aimed to investigate the role of the long non-coding RNA GAS5 in rat pheochromocytoma cells (PC12 cells). GAS5-overexpressing lentivirus was transfected into PC12 cells, and expression levels of GAS5 and C-myc were detected by real-time PCR. Ratios of cells in S phase were detected by 5-ethynyl-2′-deoxyuridine. Immunohistochemical staining was used to detect the immunoreactivity of neuron microtubule markers Tuj1, doublecortin, and microtubule-associated protein 2. Apoptosis was detected by flow cytometry, while expression of acetylcholine in cells was detected by western blot assay. We found that GAS5 can promote PC12 cells to differentiate into Tuj1-positive neuron-like cells with longer processes. In addition, cell proliferation and cell cycle were significantly suppressed by GAS5, whereas it had no effect on apoptosis of PC12 cells. Our results indicate that GAS5 could increase the expression of choline acetyltransferase and acetylcholine release. Thus, we speculate that GAS5 is beneficial to the recovery of neurons and the cholinergic nervous system.

关键词： nerve regeneration growth arrest-specific 5 PC12 cell neuron proliferation cell cycle choline acetyltransferase acetylcholine Alzheimer's disease neural regeneration

基于主成分分析协同随机森林算法的热连轧带钢宽度预测

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东北大学学报（自然科学版） 2021年第9期42卷 1268-1274,1289页

作者：丁敬国郭锦华东北大学轧制技术及连轧自动化国家重点实验室辽宁沈阳110819

为提高热连轧粗轧带钢生产过程中换钢种、换规格及换辊后的首块带钢宽度设定模型精度,本文提出一种基于主成分分析协同随机森林(PCA-RF)算法的宽度预测模型.采用主成分分析法对数据样本合理分析,通过计算特征值、主成分贡献度及累计贡... 详细信息

为提高热连轧粗轧带钢生产过程中换钢种、换规格及换辊后的首块带钢宽度设定模型精度,本文提出一种基于主成分分析协同随机森林(PCA-RF)算法的宽度预测模型.采用主成分分析法对数据样本合理分析,通过计算特征值、主成分贡献度及累计贡献度进行特征选择.在PCA筛选的变量数据集上训练最佳随机森林宽度预测模型.同时,使用支持向量机回归(SVR)、K-最近邻(KNN)模型进行对比验证.通过实际应用表明,PCA-RF各道次宽度模型R-squared值控制在99.9%~1,且96%以上样本点预测误差在-5~5 mm,从而证明该模型实现了换钢种、换规格及换辊后的首块钢宽度的高精度预测.

关键词：热连轧粗轧主成分分析特征选择宽度预测随机森林算法

Combination therapy reduces the percutaneous coronary intervention acute myocardial infarction incidence of no-reflow after primary in patients with ST-segment elevation

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Journal of Geriatric Cardiology 2015年第2期12卷 135-142页

作者： Shan-Shan ZHOU Feng TIAN Yun-Dai CHEN jing WANG Zhi-Jun SUN Jun guo Qin-hua jin Department of Cardiology Chinese PLA General Hospital Fuxing Road 28 Haidian District Beij'ing 100853 China

Background No-reflow is associated with an adverse outcome and higher mortality in patients with ST-segment elevation acute myocardial infarction （STEMI） who undergo percutaneous coronary intervention （PCI） and is considered a dynamic process characterized by multiple pathogenetic components. The aim of this study was to investigate the effectiveness of a combination therapy for the prevention of no-reflow in patient with acute myocardial infarction （AMI） undergoing primary PCI. Methods A total of 621 patients with STEMI who underwent emergency primary PCI were enrolled in this study. Patients with high risk of no-reflow （no-flow score 〉 10, by using a no-flow risk prediction model, n = 216） were randomly divided into a controlled group （n = 108） and a combination therapy group （n = 108）. Patients in the controlled group received conventional treatment, while patients in combination therapy group received high-dose （80 mg） atorvastatin pre-treatment, intracoronary administration of adenosine （140 ~tg/min per kilogram） during PCI procedure, platelet membrane glycoprotein lib/Ilia receptor antagonist （tirofiban, ***/kg bolus followed by 0.15 ~tg/kg per minute） and thrombus aspiration. Myocardial contrast echocardiography was performed to assess the myocardial perfusion 72 h after PCI. Major adverse cardiac events （MACE） were followed up for six months. Results Incidence of no-reflow in combination therapy group was 2.8%, which was similar to that in low risk group 2.7% and was significantly lower than that in control group （35.2%, P 〈 0.01）. The myocardial perfusion （A= 13） values were higher in combination therapy group than that in control group 72 h after PCI. After 6 months, there were six （6.3%） MACE events （one death, two non-fatal MIs and three revasculafizations） in combination therapy group and 12 （13.2%）（four deaths, three non-fatal MIs and five revascularizations, P 〈 0.05） in control group. Conclusions Combination of thrombus aspiration, high-dose stat

关键词： Acute myocardial infarction Myocardial contrast echocardiography No-reflow phenomenon Percutaneous coronary interven-tion ST-elevation myocardial infarction

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医疗和健康服务定性研究的理论视角

中国心理卫生杂志 2015年第7期29卷 481-486页

作者：郭金华北京大学社会学系社会学人类学研究所北京100871

医疗和健康服务研究越来越关注并期望使用定性研究,但是部分研究也存在将定性研究简化为具体的研究方法,存在相对忽略方法背后的理论视角的倾向。本文通过回顾定性研究的发展脉络揭示定性研究的理论视角及其实质。在此基础上,探讨了医... 详细信息

医疗和健康服务研究越来越关注并期望使用定性研究,但是部分研究也存在将定性研究简化为具体的研究方法,存在相对忽略方法背后的理论视角的倾向。本文通过回顾定性研究的发展脉络揭示定性研究的理论视角及其实质。在此基础上,探讨了医疗及健康服务领域引入定性研究的价值与方向。

关键词：定性研究方法视角互动