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Relationship of Somatic Cell Count with Milk Yield and Composition in Chinese Holstein Population

Agricultural Sciences in China 2010年第10期9卷 1492-1496页

作者： guo jia-zhong liu xiao-lin xu a-juan xia zhi College of Animal Science and Technology Shaanxi Key Laboratory of Molecular Biology for Agriculture Northwest A＆F University Yangling 712100 P.R.China

The objective of this study was to analyze the relationship of somatic cell count （SCC） with milk yield, fat and protein percentage, fat and protein yield using analysis of variance and correlation analysis in Chinese Holstein population. The 10 524 test-day records of 568 Chinese Holstein Cattle were obtained from 2 commercial herds in Xi＇an region of China during February 2002 to March 2009. Milk yield, fat percentage, fat and protein yield initially increased and then dropped down with parity, whereas protein percentage decreased and SCC increased. Analysis of variance showed highly significant effects of different subclasses SCC on milk yield and composition （P〈 0.01）. Compared with milk yield with SCC ≤ 200 000 cells mL-1, milk yield losses with SCC of 200 000-500 000 cells mL-1, 501000-1 000 000 cells mL-1, ≥ 1 000 000 cells mL-1 were 0.387, 0.961 and 2.351 kg, respectively. The highly significant negative correlation coefficient between somatic cell score （SCS） and milk and protein yield, milk yield and fat and protein percentage, protein percentage and fat yield were -0.084, -0.037, -0.061, -0.168, and -0.088, respectively （P〈 0.01）. The highly significant positive correlation coefficients between SCS and fat yield and fat and protein percentage, milk yield and fat and protein yield, fat percentage and protein percentage and fat yield, protein yield and protein percentage and fat yield were 0.041, 0.177, 0.105, 0.771, 0.865, 0.122, 0.568, 0.318, and 0.695, respectively （P〈 0.01）. There was no significant relationship between fat percentage and protein yield （P 〉 0.05）. The results of the present study first time provide the relevant base-line data for assessing milk production at Xi＇an region of China.

关键词： Holstein cattle milk yield milk composition somatic cell count somatic cell score

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Compound heterozygous p.L483P and p.S310G mutations in GBA1 cause type 1 adult Gaucher disease:A case report

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World Journal of Clinical Cases 2022年第36期10卷 13426-13434页

作者： xiao-ling Wen Yao-Zi Wang xia-lin Zhang jia-Qiang Tu zhi-juan Zhang xia-xia liu Hai-Yan Lu guo-Ping Hao xiao-Huan Wang lin-Hua Yang Rui-juan Zhang Department of Hematology The First People’s Hospital of YibinYibin 644000Sichuan ProvinceChina Department of Hematology The Second Hospital of Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Hematology The Third Hospital of Shanxi Medical UniversityThe Shanxi Bethune HospitalThe Shanxi Academy of Medical SciencesThe Tongji Shanxi HospitalThe Shanxi Medical UniversityTaiyuan 030032Shanxi ProvinceChina Department of Hematology The Children’s Hospital of ShanxiTaiyuan 030006Shanxi ProvinceChina

BACKGROUND Gaucher disease(GD)is caused by a GBA1 gene mutation that leads to decreased acidβ-glucosidase activity[glucocerebrosidase(GCase)].This study aimed to identify and characterise compound heterozygous mutations in GBA1 in a patient with type 1 *** SUMMARY Here,we report a rare adult-onset type 1 GD in a 46-year-old female patient with clinical manifestations of giant spleen,thrombocytopenia,and bone pain,diagnosed by enzymatic and genetic *** and whole exome sequencing revealed heterozygous missense mutations in exon 10 c.1448T>C(p.L483P)and exon 7 c.928A>G(p.S310G)of *** latter was first reported in patients with *** modelling showed that p.S310G and p.L483P were distant from the GCase active *** p.S310G mutation in domain 1 may decrease stability between theα2 andα3 helices of *** p.L483P mutation in domain 2 reduced the van der Waals force of the side chain and disrupted the C-terminalβ-*** patient was treated with imiglucerase replacement therapy,and her condition was *** The p.L483P/p.S310G novel compound heterozygous mutation underlies type 1 GD and likely affects GCase protein *** is the first description of p.S310G being associated with mild type 1 GD in the context of a coinherited p.L483P mutation.

关键词： Gaucher disease Parkinson’s disease Lipid metabolism Molecular mechanism Case report

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Acute and subchronic toxicity as well as evaluation of safety pharmacology of modified pulsatilla granules

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Journal of Integrative Agriculture 2017年第3期16卷 671-678页

作者： jia Rui-lin SONG xu guo Yu-fei YIN zhong-qiong liu Fei XIONG juan liu Qiu-yan jia Ren-yong LI Li-xia ZOU Yuan-feng YIN Li-zi HE Chang-liang LIANG xiao-xia YUE Gui-zhou College of Veterinary Medicine Sichuan Agricultural University Chengdu 611130 P.R. China Jinling Institute of Technology Nanjing 211169 P.R. China College of Science Sichuan Agricultural University Ya'an 625014 P.R.China

The present study investigated acute and subchronic toxicity and safety pharmacology of modified pulsatilla granules（MPG）to provide a basis for a comprehensive understanding of MPG *** results of acute toxicity testing showed that the median lethal dose of MPG was more than 5 000 mg kg^-1,suggesting that MPG was considered as practically *** subchronic toxicity study for 30 days was conducted by daily oral administration at doses of 375,750 and 1 500 mg kg^-1 in Sprague-Dawley *** results of subchronic toxicity study showed that the body weight and relative organ weight were not significantly changed by administration of *** clinical chemistry study showed that MPG could induce kidney and liver *** histopathological,mild lesions in liver and kidney were also observed,suggesting that the liver and kidney might be potential target organs of *** the safety pharmacology study,MPG did not exhibited any side effects to rats in cardiovascular system,respiratory system and central nervous *** results suggested that MPG could be considered safe for veterinary use.

关键词： modified pulsatilla granules acute toxicity subchronic toxicity safety pharmacology

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Towards the full realization of the RIBLL2 beam line at the HIRFL-CSR complex

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Science Bulletin 2018年第2期63卷 78-80页

作者： Bao-Hua Sun jian-Wei Zhao xue-Heng Zhang Li-Na Sheng zhi-Yu Sun Isao Tanihata Satoru Terashima Yong Zheng Li-Hua Zhu Li-Min Duan liu-Chun He Rong-jiang Hu Guang-Shuai Li Wen-jian lin Wei-Ping lin Chuan-Ye liu zhong liu Chen-Gui Lu Xin-Wen Ma Li-Jun Mao Yi Tian Feng Wang Meng Wang Shi-Tao Wang jia-Wen xia xiao-Dong xu Hu-Shan xu zhi-guo xu jian-Cheng Yang Da-Yu Yin You-Jin Yuan Wen-Long Zhan Yu-Hu Zhang xiao-Hong Zhou School of Physics and Nuclear Energy Engineering Beihang University Beijing 100191 China International Research Center for Nuclei and Particles in the Cosmos Beijing 100191 China Institute of Modern Physics Chinese Academy of Sciences Lanzhou 730000 China

More than 99%of the mass in the visible universe—the material that makes up ourselves,our planet,stars—is in the atomic *** the matter has existed for billions of years,only over the past few decades have we had the tools and the knowledge necessary to get a basic understanding of the structure and dynamic of *** physicists around the world have made tremendous strides by initiating a broad range of key

关键词： HIRFL-CSR 横梁建筑群排队原子核物理学家试验性世界

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Grape Seed Proanthocyanidin Extract Alleviates Arsenic-induced Oxidative Reproductive Toxicity in Male Mice

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Biomedical and Environmental Sciences 2015年第4期28卷 272-280页

作者： LI Shu Gang DING Yu Song NIU Qiang xu Shang zhi PANG Li juan MA Ru lin JING Ming xia FENG Gang ling liu jia Ming guo Shu xia Department of Public Health and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education) Shihezi University School of Medicine

Objective To determine the ability of grape seed proanthocyanidin extract （GSPE） in alleviating arsenic-induced reproductive toxicity. Methods Sixty male Kunming mice received the following treatments by gavage： normal saline solution （control）; arsenic trioxide （ATO; 4 mg/kg）; GSPE （400 mg/kg）; ATO＋GSPE （100 mg/kg）; ATO＋GSPE （200 mg/kg） and ATO＋GSPE （400 mg/kg）. Thereafter, the mice were sacrificed and weighed, and the testis was examined for pathological changes. Nuclear factor （erythroid-derived 2）-like 2 （Nrf2）, heme oxygenase 1 （HO1）, glutathione S-transferase （GST）, NAD（P）H dehydrogenase, and quinone 1 [NQO1） expression in the testis was detected by real-time PCR. Superoxide dismutase （SOD）, glutathione （GSH）, total antioxidative capability （T-AOC）, malondialdehyde （MDA）, 8-hydroxydeoxyguanosine （8-OHdG）, and reproductive indexes were analyzed. Results ATO-treated mice showed a significantly decreased sperm count and testis somatic index and activity levels of SOD, GSH, and T-AOC than control group. Compared to the ATO-treated group, ATO ＋GSPE group showed recovery of the measured parameters. Mice treated with ATO＋high-dose GSPE showed the highest level of mRNA expression of Nrf2, HO, NO.O1, and GST. Conclusion GSPE alleviates oxidative stress damage in mouse testis by activating Nrf2 signaling, thus counteracting arsenic-induced reproductive toxicity.

关键词： Grape seed proanthocyanidin extract Arsenic Reproductive toxicity Nrf2 signaling

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TNF-α基因多态性及其与奶牛乳房炎的相关性分析

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遗传 2010年第9期32卷 929-934页

作者：徐阿娟刘小林郭家中夏志西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室杨凌712100

以417头中国荷斯坦奶牛为研究对象,根据体细胞评分(Somatic cell score,SCS)的大小将该奶牛群体划分为感染牛群(100头)和健康牛群(317头)。通过PCR-RFLP和CRS-RFLP方法检测了肿瘤坏死因子α(Tumor necrosis factor-alpha,TNF-α)基因在... 详细信息

以417头中国荷斯坦奶牛为研究对象,根据体细胞评分(Somatic cell score,SCS)的大小将该奶牛群体划分为感染牛群(100头)和健康牛群(317头)。通过PCR-RFLP和CRS-RFLP方法检测了肿瘤坏死因子α(Tumor necrosis factor-alpha,TNF-α)基因在荷斯坦奶牛群体中的多态性,并分析这些多态位点和奶牛乳房炎的相关性。研究发现了3个单核苷酸多态位点(Single-nucleotide polymorphism,SNP):第2外显子39bp处G→A的突变;第4外显子293bp处C→T的突变;5′侧翼区(5′-flanking region,5′UTR)C→G的突变。这3个突变位点分别是DraⅠ、AfaⅠ和DdeⅠ限制性内切酶的酶切多态位点,其中DraⅠ为创造酶切位点。经过基因型分析与χ2检验表明:3个酶切多态位点在荷斯坦奶牛群中均未达到Hardy-Weinberg平衡状态。运用SPSS13.0软件,采用最小二乘拟合线性模型分析3个酶切多态位点与SCS的关系,结果表明:AA基因型个体在DraⅠ酶切位点中的SCS显著大于BB及AB基因型个体(P<0.05),BB基因型表现出乳房炎抗性。AfaⅠ酶切位点中BB基因型个体的SCS显著大于AA及AB基因型个体(P<0.05),AA基因型表现出乳房炎抗性。DdeⅠ酶切位点中,AB基因型个体的SCS显著低于AA基因型个体(P<0.05),AB基因型为优良基因型。因此BB、AA、AB基因型分别为DraⅠ、AfaⅠ、DdeⅠ酶切位点中的优良基因型,可作为分子标记应用于奶牛乳房炎抗性筛选。

关键词： TNF-α基因 PCR-RFLP 多态性乳房炎相关性

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奶牛体细胞评分的变化规律研究

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西北农林科技大学学报（自然科学版） 2010年第6期38卷 67-70页

作者：郭家中刘小林徐阿娟夏志西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室陕西杨凌712100

【目的】研究影响奶牛体细胞评分(SCS)的因素,为奶牛体细胞评分性状的遗传分析和乳房炎防治提供依据。【方法】收集2000-10-2009-03西安地区2个奶牛场575头中国荷斯坦牛的10 620条奶牛群改良(DHI)测定记录,应用最小二乘法原理,配合线性... 详细信息

【目的】研究影响奶牛体细胞评分(SCS)的因素,为奶牛体细胞评分性状的遗传分析和乳房炎防治提供依据。【方法】收集2000-10-2009-03西安地区2个奶牛场575头中国荷斯坦牛的10 620条奶牛群改良(DHI)测定记录,应用最小二乘法原理,配合线性固定效应模型,分析了牧场、年份、月份、胎次、泌乳月份等因素对SCS的影响。【结果】不同水平的牧场、年份、月份、胎次、泌乳月份对SCS影响的差异显著性分析表明,生产管理因素显著影响牛群的SCS(P<0.05);随着DHI项目的实施,牛群SCS值逐年显著降低(P<0.05);夏季高温和冬季低温显著提高SCS值(P<0.05);伴随胎次和泌乳月份的增加,SCS不断升高(P<0.05)。【结论】通过改善挤奶技术、加强卫生管理等措施,能够降低SCS。在体细胞评分性状的遗传分析中,必须考虑环境和生理因素的效应。

关键词：中国荷斯坦牛体细胞评分变化规律

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奶牛CRP基因多态性与奶牛乳腺炎及乳质乳量的相关性研究及生物信息学分析

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中国兽医学报 2011年第4期31卷 609-613,618页

作者：夏志刘小林徐阿娟郭家中贺花西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室陕西杨凌712100

选用400头中国荷斯坦奶牛取血样400份,每份各2管。根据奶牛CRP基因序列设计2对引物,利用PCR-SSCP技术检测CRP基因的多态性,并用透射免疫比浊法测定奶牛血清CRP水平,然后运用SPSS等统计学软件对其与乳房炎及乳质、乳量的关系进行统计分... 详细信息

选用400头中国荷斯坦奶牛取血样400份,每份各2管。根据奶牛CRP基因序列设计2对引物,利用PCR-SSCP技术检测CRP基因的多态性,并用透射免疫比浊法测定奶牛血清CRP水平,然后运用SPSS等统计学软件对其与乳房炎及乳质、乳量的关系进行统计分析。结果显示,在第1对引物的扩增片段中检测到多态位点,出现3种基因型(AA、AB、BB),第2对引物的扩增片段中并未检测到多态位点。将第1对引物所扩片段的不同基因型与奶牛乳腺炎的相关性作关联分析,结果3种不同基因型奶牛的体细胞数、乳质率、蛋白率、305d产奶量均无显著差异,但患乳房炎奶牛个体全为AA型。而CRP含量检测结果可知患乳房炎的奶牛与正常个体之间的血清CRP含量并无显著差异。

关键词： C反应蛋白多态性乳房炎抗性易感性感染奶牛

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Metatranscriptome of human lung microbial communities in a cohort of mechanically ventilated COVID-19 Omicron patients

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Signal Transduction and Targeted Therapy 2023年第12期8卷 5919-5930页

作者： lin Wang jia-Bao Cao Bin-Bin xia Yue-juan Li xuan Zhang guo-Xin Mo Rui-juan Wang Si-Qi guo Yu-Qing Zhang Kun xiao Guang-Fa Zhu Peng-Fei liu Li-Cheng Song Xi-Hui Ma Ping-Chao xiang jiang Wang Yu-Hong liu Fei Xie xu-Dong Zhang xiang-Xin Li Wan-Lu Sun Yan Cao Kai-Fei Wang Wen-Hui Zhang Wei-Chao Zhao Peng Yan Ji-Chao Chen Yu-Wei Yang zhong-Kuo Yu Jing-Si Tang Li xiao Jie-Min Zhou Li-Xin Xie Jun Wang College of Pulmonary&Critical Care Medicine 8th Medical Center of Chinese PLA General HospitalBeijing 100091China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Faculty of Biological Science and Technology Baotou Teacher’s CollegeBaotou 014030China Department of Respiratory Medicine PLA Strategic Support Force Medical CenterBeijing 100101China Department of Respiratory and Critical Care Medicine Beijing Anzhen HospitalCapital Medical UniversityBeijing Institute of HeartLung and Blood Vessel DiseasesBeijing 100029China Respiratory Research Institute Department of Pulmonary&Critical Care MedicineBeijing Key Laboratory of OTIRthe 8th Medical Center of Chinese PLA General HospitalBeijing 100091China Shougang hospital of Peking University Beijing 100144China Department of Respiratory Medicine Beijing Changping HospitalBeijing 102200China Department of Respiratory and Critical Care Medicine Beijing Institute of Respiratory Medicine and Beijing Chao-Yang HospitalCapital Medical UniversityBeijing 100020China Pulmonary research institute Senior Department of Respiratory and Critical Care Medicinethe 8th medical center of Chinese PLA general hospitalBeijing 100091China College of Pulmonary&Critical Care Medicine Chinese PLA General HospitalBeijing 100853China China Aerospace Science&Industry Corporation 731 hospital Beijing 100074China Department of Respiratory and Critical Care Medicine Aerospace Center HospitalBeijing 100049China Vision Medicals Center for Infectious Diseases Guangzhou 510700China

The Omicron variant of the severe acute respiratory syndrome coronavirus 2(SARS‑CoV‑2)infected a substantial proportion of Chinese population,and understanding the factors underlying the severity of the disease and fatality is valuable for future prevention and clinical *** recruited 64 patients with invasive ventilation for COVID-19 and performed metatranscriptomic sequencing to profile host transcriptomic profiles,plus viral,bacterial,and fungal content,as well as virulence factors and examined their relationships to 28-day mortality were *** addition,the bronchoalveolar lavage fluid(BALF)samples from invasive ventilated hospital/community-acquired pneumonia patients(HAP/CAP)sampled in 2019 were included for *** analysis revealed that all Omicron strains belong to BA.5 and BF.7 sub-lineages,with no difference in 28-day mortality between *** to HAP/CAP cohort,invasive ventilated COVID-19 patients have distinct host transcriptomic and microbial signatures in the lower respiratory tract;and in the COVID-19 non-survivors,we found significantly lower gene expressions in pathways related viral processes and positive regulation of protein localization to plasma membrane,higher abundance of opportunistic pathogens including bacterial Alloprevotella,Caulobacter,Escherichia-Shigella,Ralstonia and fungal Aspergillus sydowii and Penicillium *** analysis further revealed significant associations between host immune responses and microbial compositions,besides synergy within viral,bacterial,and fungal *** study presents the relationships of lower respiratory tract microbiome and transcriptome in invasive ventilated COVID-19 patients,providing the basis for future clinical treatment and reduction of fatality.

关键词： patients mortality prevention

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奶牛乳房炎对奶牛血清若干生化指标的影响

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中国乳品工业 2010年第7期38卷 32-34,64页

作者：夏志刘小林郭家中徐阿娟西北农林科技大学动物科技学院陕西杨凌712100

为研究乳房炎对奶牛生理代谢的影响,进而为深入分析乳房炎的致病理提供血液学依据。采用生化分析仪,对奶牛乳房炎感染组和阴性对照组的多项血清指标进行测定。结果表明,感染组和对照组相比,白蛋白(ALB)和丙氨酸氨基转移酶(ALT)等指标差... 详细信息

为研究乳房炎对奶牛生理代谢的影响,进而为深入分析乳房炎的致病理提供血液学依据。采用生化分析仪,对奶牛乳房炎感染组和阴性对照组的多项血清指标进行测定。结果表明,感染组和对照组相比,白蛋白(ALB)和丙氨酸氨基转移酶(ALT)等指标差异显著(P<0.05),其中白蛋白差异极显著(P<0.01);而超敏C反应蛋白(Hs-CRP)、天冬氨酸氨基转移酶(AST)、葡萄糖(GLU)和总胆红素(TBIL)质量分数在对照组和感染组,差异不显著。因此,白蛋白和丙氨酸氨基转移酶可作为奶牛乳房炎的血液学指示指标。

关键词：乳房炎生化指标血清奶牛

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