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云南普朗铜矿周边优势草本根际土壤养分与细菌群落结构特征

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微生物学通报 2024年第5期51卷 1566-1582页

作者：李伟李博湛方栋郭佳玮杨继清董恒文杨继华中国有色金属工业昆明勘察设计研究院有限公司云南昆明650051 云南农业大学资源与环境学院云南昆明650201 云南农业大学建筑工程学院云南昆明650201

【背景】矿产开采会对矿场周边生态环境造成严重影响,探究矿场周边土壤养分及细菌群落结构特点对矿区周边植被恢复具有重要意义。【目的】探究云南香格里拉普朗铜矿矿场周边不同小生境的土壤养分与细菌群落结构特征。【方法】以普朗铜... 详细信息

【背景】矿产开采会对矿场周边生态环境造成严重影响,探究矿场周边土壤养分及细菌群落结构特点对矿区周边植被恢复具有重要意义。【目的】探究云南香格里拉普朗铜矿矿场周边不同小生境的土壤养分与细菌群落结构特征。【方法】以普朗铜矿周边4个小生境(亚高山草甸、山谷草地、矿山林地和亚高山林地)夏季和冬季优势草本植物的根际土壤为研究对象,分析根际土壤的养分与细菌群落结构特征。【结果】矿山林地的草本根际土壤养分含量最高,总体处于中等及以上水平。普朗铜矿周边4个小生境土壤细菌的优势菌门分别为酸杆菌门(Acidobacteriota)、放线菌门(Actinobacteriota)和变形菌门(Proteobacteria),相对丰度占比为11.7%-60.4%,优势菌属分别为慢生根瘤菌属(Bradyrhizobium)、阿菲波菌属(Afipia)和节杆菌属(Arthrobacter),相对丰度占比为6.1%-53.6%。Mantel分析结果表明,在细菌属水平分类下,土壤有机质对土壤细菌群落有显著影响。【结论】夏季土壤细菌群落相对丰度显著高于冬季(P0.05)。本研究将有助于拓展对矿山生态系统的认识,可为矿区植被恢复和土壤污染治理提供理论依据。

关键词：普朗铜矿亚高山草本土壤细菌群落养分多样性

Development of an Efficient CRISPR/Cas9 System in Fusarium verticillioides and Its Application in Reducing Mycotoxin Contamination

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Development of an Efficient CRISPR/Cas9 System in Fusarium v...

玫芦消痤膏治疗湿疹作用机制的网络药理学研究

中国植物病理学会2024年学术年会

作者： TANG Tingting DING Yi guo Wei Institute of Food Science and Technology Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport ProcessMinistry of Agriculture and Rural Affairs

Fusarium verticillioides is a globally recognized and highly impactful fungal pathogen of maize,causing yield losses and producing harmful mycotoxins that pose a threat to human and animal ***,the genetictools available for studying this crucial fungus are currently limited in comparison with other important fungal *** address this,an efficient CRISPR/Cas9 genome editing system based on an autonomously replicating plasmid with an AMA1 sequence was established in this ***,gene disruption ofpyrG and pyrE via non-homologous end-joining（NHEJ） pathway was successfully achieved,with efficiency ranging from 66% to 100%.Secondly,precise gene deletions were achieved with remarkable efficiency using a dual sgRNA expression ***,the developed genome editing system can be applied to generate designer chromosomes in ***,as evidenced by the deletion of a crucial 38 kb fragment required for fumonisin ***,the pyrG recycling system has been established and successfully applied in ***,the developed ΔFUM1 and ΔFUM mutants can serve as biocontrol agents to reduce the Fumonisin B（FB） contamination produced by the *** together,these significant advancements in geneticmanipulation and biocontrol strategies provide valuable tools for studying and mitigating the impact of *** on maize crops.

关键词： CRISPR/Cas9 Fusarium verticillioides fumonisin uracil auxotrophic selection system atoxigenic strain

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西部中医药 2024年第7期37卷 43-50页

作者：欧洪郭静王江瑞成都中医药大学四川成都610075 成都中医药大学附属医院四川成都610075 贵州良济药业有限公司贵州龙里551200

目的:研究苗药玫芦消痤膏治疗湿疹的作用机制,探索玫芦消痤膏治疗湿疹的作用靶标及信号途径。方法:在中药系统药理学数据库和分析平台,以及多个数据库中,联合检索筛选玫芦消痤膏活性成分-作用靶点,基因名注释使用UniProt数据库,并在OMI... 详细信息

目的:研究苗药玫芦消痤膏治疗湿疹的作用机制,探索玫芦消痤膏治疗湿疹的作用靶标及信号途径。方法:在中药系统药理学数据库和分析平台,以及多个数据库中,联合检索筛选玫芦消痤膏活性成分-作用靶点,基因名注释使用UniProt数据库,并在OMIM数据库、GeneCards数据库、DrugBank数据库中检索湿疹的相关疾病靶标,再采用Rversion 3.6.2软件及Cytoscape 3.7.1软件构建玫芦消痤膏治疗湿疹的成分-作用靶点-通路网络,最后利用R语言中Bioc Manager包进行基因本体功能注释及KEGG通路富集分析。结果:经筛选得到玫芦消痤膏活性成分35个,槐定碱、槲皮素、苦参碱、木犀草素、鞣花酸、花生四烯酸、芦荟大黄素等是关键活性成分;作用靶标基因97个,核心蛋白有丝裂原活化蛋白激酶3前列腺素内过氧化物合酶,丝裂原活化蛋白激酶1、内皮一氧化氮合成酶、激素受体1、雄激素受体、脑源性神经营养因子、转录因子、过氧化物酶体增生激活受体等,参与调控高级糖基化终末产物-受体、肿瘤坏死因子、白细胞介素17、丝裂原活化蛋白激酶、PI3K-Akt等信号通路,从而发挥抗炎等作用。结论:玫芦消痤膏能有效缓解湿疹症状,可能与肿瘤坏死因子、PI3K-Akt等经典炎症通路有关,为后续分析玫芦消痤膏的药理机制和实验验证提供了理论支撑。

关键词：湿疹玫芦消痤膏网络药理学靶点通路

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基于LSTM深度学习网络的分布动载荷识别

振动与冲击 2024年第11期43卷 126-134页

作者：郭安丰吴邵庆东南大学土木工程学院南京211189 东南大学江苏省工程力学分析重点实验室南京211189

提出一种基于LSTM深度学习网络的分布动载荷识别新方法。首先,建立结构有限元模型并对载荷作用区域进行平面化和子区域网格划分,构建子区域上以形函数形式分布的动载荷和有限元模型节点动响应之间的传递关系,建立节点处应变动响应与对... 详细信息

提出一种基于LSTM深度学习网络的分布动载荷识别新方法。首先,建立结构有限元模型并对载荷作用区域进行平面化和子区域网格划分,构建子区域上以形函数形式分布的动载荷和有限元模型节点动响应之间的传递关系,建立节点处应变动响应与对应子区域上分布动载荷的样本库;其次,利用Meyer小波对样本库中的时域样本进行特征提取,并基于LSTM深度学习网络训练子区域上分布动载荷与有限元模型节点应变动响应的传递关系;最后,开展了数值仿真研究,利用有限元模型仿真应变动响应识别了三维壁板结构表面的分布动载荷,验证了所提出方法的有效性。研究旨在为服役状态下壁板结构上动载荷环境预示提供技术支撑。

关键词：分布动载荷载荷识别深度学习网络小波变换仿真研究

Definition and Algorithms for Reliable Steiner Tree Problem

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Journal of Systems Science & Complexity 2015年第4期28卷 876-886页

作者： TANG Yaohua yang Wenguo guo Tiande School of Mathematics University of Chinese Academy of Sciences

This paper considers a new form of the Steiner tree problem that is more practical and reliable,which we call Reliable Steiner Tree(RST)*** authors give a detailed definition for this new problem and design both an exact algorithm and an approximation algorithm for *** definition is based on the reliability of full components instead of Steiner *** task is thus to find the most reliable full components to make up an optimum reliable Steiner *** exact algorithm designed for this problem utilizes a dynamic programming *** approximation algorithm designed in this paper exploits a local search strategy that looks for the best full component according to a selection function at a time.

关键词： Approximation algorithm exact algorithm reliability steiner tree.

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腐植酸接枝共聚物的研究进展及应用前景

应用化工 2024年第4期53卷 873-876,882页

作者：杨会国马丽萍张彦彬谢肥东化全县侯翠红郑州大学化工学院国家钙镁磷复合肥技术研究推广中心河南郑州450000

综述了接枝共聚物的发展现状与腐植酸类共聚物的研究进展。腐植酸含有丰富的含氧官能团,如羧基、酚羟基等基团,使得腐植酸分子具有氧化还原、亲水、络合、吸附、分散以及易于接枝共聚改性的能力。从不同用途介绍腐植酸类共聚物,因其具... 详细信息

综述了接枝共聚物的发展现状与腐植酸类共聚物的研究进展。腐植酸含有丰富的含氧官能团,如羧基、酚羟基等基团,使得腐植酸分子具有氧化还原、亲水、络合、吸附、分散以及易于接枝共聚改性的能力。从不同用途介绍腐植酸类共聚物,因其具有成本低、绿色、安全等特性,在工业、农业、畜牧业、林业以及医药等方面都有着广阔的应用前景。

关键词：腐植酸黄腐酸接枝改性螯合

Effect of phosphatase and tensin homolog-induced putative kinase 1/E3 ubiquitin ligase Parkin mediated mitochondrial autophagy on chronic kidney disease myocardial injury and the intervention mechanism of Shenshuai recipe(肾衰方)

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Journal of Traditional Chinese Medicine 2024年第5期44卷 934-943页

作者： ZHANG Gedi LIU Gengxin guo Min LUO Fuli YAN Ziyou GE Wei School of Graduate Jiangxi University of Chinese Medicine Department of Nephrology Affiliated Hospital of Jiangxi University of Chinese Medicine Department of Anorectal Affiliated Hospital of Jiangxi University of Chinese Medicine

OBJECTIVE: To study whether Shenshuai recipe(肾衰方, SSR) can play a protective role on chronic kidney disease myocardial injury model through phosphatase and tensin homolog-induced putative kinase 1(PINK1)/E3 ubiquitin ligase Parkin(Parkin) mitochondrial autophagy ***: Forty-eight nephrectomized rats were randomly divided into six groups: sham-operated group,model group, Benazepril group, low, medium and highdose groups of SSR. The rats were given the corresponding intervention for six weeks, then were *** was examined by enzyme linked immunosorbent assay(ELISA). Cardiac ultrasound was used to detect cardiac function in 5/6 nephrectomized rats. Myocardial tissue was examined by light and electron microscopy;PINK1, Parkin, microtubule-associated protein 1 light chain 3 II(LC3B), sequestosome 1(P62), BECN1(Beclin-1)and dynamin-related protein 1(Drp-1) were measured by real time polymerase chain reaction(RT-PCR), Western blot(WB) and immunohistochemistry(IHC).RESULTS: The expression levels of blood urea nitrogen(BUN) and creatinine(SCr) in the model group were significantly higher than those in the sham-operated group, indicating that modeling was successful. SSR can protect myocardium by reducing the relative expression of creatine kinase myocardial isoenzyme and hypersensitivity cardiac troponin I(P < 0.05). SSR can improve cardiac function in rats after ultrasound *** can improve the pathological manifestations of myocardial tissue after Masson staining. SSR can increase the number of autophagosomes and autophagiclysosomes in 5/6 nephrectomized rats(P <0.05). Determined by RT-PCR, WB and IHC, SSR can increase the relative expression of PINK1, Parkin, and LC3B(P < 0.05), and decrease the relative expression of P62, Beclin-1 and Drp-1(P < 0.05).CONCLUSIONS: The PINK1/Parkin mitochondrial autophagy pathway in myocardial tissues in 5/6 nephrectomy CKD myocardial injury rats was *** can activate PINK1/Parkin mitochondrial autophag

关键词： PTEN phosphohydrolase Shenshuai recipe mitochondrial autophagy myocardial injury renal insufficiency, chronic ubiquitin-protein ligases

致密储层差异化成岩作用及其对烃类充注过程的影响——以鄂尔多斯盆地延安地区下二叠统山西组1段为例

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天然气地球科学 2024年第10期35卷 1777-1788页

作者：郭兰郭顺丁超马志武延长油田股份有限公司陕西延安716000 陕西延长石油(集团)有限责任公司资源与勘探开发部陕西西安710075 西安石油大学地球科学与工程学院陕西西安710065 西安石油大学陕西省油气成藏地质学重点实验室陕西西安710065

针对鄂尔多斯盆地非常规天然气勘探亟待解决的关键地质问题,揭示储层成岩致密化与油气充注的动态过程和关系,利用铸体薄片观察、X射线衍射、扫描电镜分析,细致对比不同类型岩石相的成岩作用类型及特征的差异性。通过流体包裹体岩相学、... 详细信息

针对鄂尔多斯盆地非常规天然气勘探亟待解决的关键地质问题,揭示储层成岩致密化与油气充注的动态过程和关系,利用铸体薄片观察、X射线衍射、扫描电镜分析,细致对比不同类型岩石相的成岩作用类型及特征的差异性。通过流体包裹体岩相学、显微测温及激光拉曼探针测试,结合单井埋藏史和热史模拟,确定烃类充注期次和年代。综合利用成岩矿物间及与烃类间镜下结构关系观察,建立含气储层成岩—油气充注演化序列。结果表明:山西组1段主要发育2期烃类充注,第一期发生在晚三叠世—早、中侏罗世(220~170 Ma),第二期发生在晚侏罗世—早白垩世(160~100 Ma)。不同类型岩石的成岩作用、致密化过程及其与烃类充注的序列明显存在差异,富石英贫塑性岩屑砂岩在第二期烃类充注前物性较好,中成岩阶段烃类充注时边致密边成藏,富塑性岩屑砂岩早成岩阶段已基本被压实,而碳酸盐致密胶结砂岩由于方解石和菱铁矿的形成,储层致密化导致第二期烃类无法进入。深入理解致密储层差异成岩过程与油气充注关系,对筛选天然气有利区具有重要意义。

关键词：延安地区山西组非常规储层成岩演化成藏时序

Origin and structures of solar eruptions Ⅰ: Magnetic flux rope

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Science China Earth Sciences 2017年第8期60卷 1383-1407页

作者： CHENG Xin guo yang DING MingDe School of Astronomy and Space Science Nanjing University Key Laboratory for Modern Astronomy and Astrophysics (Nanjing University) Ministry of Education

Coronal mass ejections(CMEs) and solar flares are the large-scale and most energetic eruptive phenomena in our solar system and able to release a large quantity of plasma and magnetic flux from the solar atmosphere into the solar wind. When these high-speed magnetized plasmas along with the energetic particles arrive at the Earth, they may interact with the magnetosphere and ionosphere, and seriously affect the safety of human high-tech activities in outer space. The travel time of a CME to 1 AU is about 1–3 days, while energetic particles from the eruptions arrive even earlier. An efficient forecast of these phenomena therefore requires a clear detection of CMEs/flares at the stage as early as possible. To estimate the possibility of an eruption leading to a CME/flare, we need to elucidate some fundamental but elusive processes including in particular the origin and structures of CMEs/flares. Understanding these processes can not only improve the prediction of the occurrence of CMEs/flares and their effects on geospace and the heliosphere but also help understand the mass ejections and flares on other solar-type stars. The main purpose of this review is to address the origin and early structures of CMEs/flares, from multi-wavelength observational perspective. First of all, we start with the ongoing debate of whether the pre-eruptive configuration, i.e., a helical magnetic flux rope(MFR), of CMEs/flares exists before the eruption and then emphatically introduce observational manifestations of the MFR. Secondly, we elaborate on the possible formation mechanisms of the MFR through distinct ways. Thirdly, we discuss the initiation of the MFR and associated dynamics during its evolution toward the CME/flare. Finally, we come to some conclusions and put forward some prospects in the future.

关键词： Coronal mass ejections Flares Magnetic flux ropes Magnetic field EUV/UV emissions Photosphere Corona Particle acceleration