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A single nucleotide substitution in the MATE transporter gene regulates plastochron and the many noded dwarf phenotype in barley(Hordeum vulgare L.)

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Journal of Integrative Agriculture 2023年第8期22卷 2295-2305页

作者： guo bao-jian SUN Hong-wei QI jiang HUANG Xin-yu HONG Yi HOU jian Lü Chao WANG Yu-lin WANG Fei-fei ZHU Juan guo Gang-gang XU Ru-gen jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain CropsYangzhou UniversityYangzhou 225009P.R.China State Key Laboratory of Barley and Yak Germplasm Resources and Genetic Improvement/Tibet Academy of Agricultural and Animal Husbandry Sciences(TAAAS) Lhasa 850002P.R.China Key Laboratory of Crop Germplasm Resources and Utilization Ministry of Agriculture/National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China

In higher plants,the shoot apical meristem produces lateral organs in a regular spacing(phyllotaxy)and timing(plastochron).The molecular analysis of mutants associated with phyllotaxy and plastochron would increase our understanding of the mechanism of shoot architecture *** this study,we identified mutant mnd8ynp5 that shows an increased rate of leaf emergence and a larger number of nodes in combination with a dwarfed growth habit from an EMS-treated population of the elite barley cultivar Yangnongpi *** a map-based cloning strategy,the mnd8 gene was narrowed down to a 6.7-kb genomic interval on the long arm of chromosome *** analysis revealed that a C to T single-nucleotide mutation occurred at the first exon(position 953)of HORVU5Hr1G118820,leading to an alanine(Ala)to valine(Val)substitution at the 318th amino acid ***,HORVU5Hr1G118820 was defined as the candidate gene of MND8 encoding 514 amino acids and containing two multidrug and toxic compound extrusion(MATE)*** is highly homologous to maize Bige1and has a conserved function in the regulation of plant development by controlling the leaf initiation *** of modern barely varieties showed that Hap-1 was the dominant haplotype and was selected in barley breeding around the ***,our results indicated that mnd8ynp5 is a novel allele of the HORVU5Hr1G118820 gene that is possibly responsible for the shortened plastochron and many noded dwarf phenotype in barley.

关键词： barley EMS plastochron many noded dwarf MATE transporter shoot architecture

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Proteomic Identification of Rhythmic Proteins in Maize Seedling Leaves

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Journal of Integrative Agriculture 2012年第12期11卷 1958-1967页

作者： FENG Wan-jun guo bao-jian YAO Ying-yin PENG Hui-ru SUN Qi-xin NI Zhong-fu Key Laboratory of Crop Heterosis and Utilization Ministry of Education/State Key Laboratory for AgrobiotechnologyMinistry of Education/Key Laboratory of Crop Genomics and Genetic ImprovementMinistry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/College of Agriculture and Biotechnology China Agricultural University National Plant Gene Research Centre

Plant leaves respond to day/night cycling in a number of physiological ways. At the mRNA level, the expression of some genes changes during the 24 h period. To determine which proteins exhibited a rhythmic pattern of expression, proteomic profiles in maize seedling leaves were analyzed by high-throughput two-dimensional gel electrophoresis, combined with MALDI-TOF MS technology. Of the 464 proteins that were detected with silver staining in a pH range of 4-7, 17 （3.66%） showed clock rhythmicity in their abundance. These proteins belonged to diverse functional groups and proteins involved in photosynthesis and carbon metabolism were over-represented. These findings provide a new perspective on the relationship between the physiological functions of leaves and the clock rhythmic system.

关键词： proteomics rhythmic proteins maize leaves mass spectrometry two-dimensional gel electrophoresis

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Tetramethylpyrazine nitrone improved mitochondrial dysfunction, downregulated α-synuclein, and reduced neurodegeneration in Parkinson disease models

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中国药理学与毒理学杂志 2019年第10期 843-844页

作者： guo bao-jian ZHENG Cheng-you QIU Xiao-ling ZHANG Zai-jun Institute of New Drug Research and Guangzhou Key Laboratory of Innovative Chemical Drug Research in Cardiocerebrovascular Diseases Jinan University College of Pharmacy

OBJECTIVE The peroxisome proliferatoractivated receptor γ co-activator 1α(PGC-1α) and nuclear factor(erythroid-derived 2)-like 2(Nrf2) are key regulators controlling antioxidant defense, mitochondrial biogenesis and cellular proteostasis. Dysfunction of any of these processes has been implicated in the pathogenesis of Parkinson disease(PD). Activation of PGC-1α/Nrf2 might improve mitochondrial dysfunction, promote α-synuclein(α-syn) clearance, and attenuate degeneration of nigral dopaminergic neurons in PD. To test this, we used tetramethylpyrazine nitrone(TBN), a potent free radical scavenger, to activate PGC-1α/Nrf2 pathway. METHODS 1-Methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine(MPTP)-induced mouse model, unilateral intrastriatal injection of 6-hydroxydopamine(6-OHDA)-induced rat model, and transgenic mice overexpression of human A53 T mutant α-synuclein were used to evaluate TBN's therapeutic efficacy and to explore its mechanisms of action. RESULTS TBN protected against 1-methyl-4-phenylpyridinium(MPP+) and 6-OHDA insult in cultured primary midbrain neurons. TBN promoted α-syn clearance by autophagy and proteasomal pathways in the cell models overexpressed human A53 T mutant α-syn. In MPTP-treated mouse and unilateral 6-OHDA-lesioned rat, as well as in α-syn transgenic mouse model, TBN improved motor impairment, increased survive of nigra dopaminergic neurons, and elevated striatal dopamine level, while decreased the products of oxidative ***, TBN down-regulated α-syn level in the brain and serum of α-syn transgenic mice. These improvements in vitro and in vivo were associated with activation of PGC-1α/Nrf2 signal pathway, resulting in reduced oxidative stress, enhanced mitochondrial function, and increased protein clearance. CONCLUSION Our work demonstrates that activation of PGC-1α/Nrf2 by TBN can maintain the survival of nigral dopaminergic neurons, and might be disease-modifying in PD models, providing the critical rationale for further devel

关键词： tetramethylpyrazine nitrone Parkinson disease peroxisome proliferator-activated receptor γ co-activator 1α nuclear factor(erythroid-derived 2)-like 2 α-synuclein

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丝裂霉素C与卡介苗膀胱灌注预防膀胱癌术后复发的疗效观察

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中国现代应用药学 2007年第z1期24卷 650-652页

作者：包国建浙江东阳市中医院浙江东阳322100

目的探讨丝裂霉素C(MMC)和卡介苗(BCG)膀胱腔内序贯组合灌注对预防膀胱癌术后复发的疗效。方法38例膀胱癌患者经尿道电切术(TURBT)或膀胱部分切除术后分两组:①MMC和BCG组合(20例):术后1周内先用MMC膀胱腔内灌注,第2周后用BCG膀胱腔内... 详细信息

目的探讨丝裂霉素C(MMC)和卡介苗(BCG)膀胱腔内序贯组合灌注对预防膀胱癌术后复发的疗效。方法38例膀胱癌患者经尿道电切术(TURBT)或膀胱部分切除术后分两组:①MMC和BCG组合(20例):术后1周内先用MMC膀胱腔内灌注,第2周后用BCG膀胱腔内灌注。以后二药交替使用,每周1次。②单用BCG组(18例)术后随访24个月,单用BCG组3例分别于术后6,8,11个复发、复发率16%(3/18),MMC和BCG组合无复发。两组均无严重并发症和不良反应。结论MMC和BCG组合定期磅胱内灌注的化学免疫疗法对预防膀胱癌术后复发疗效肯定。

关键词：膀胱癌丝裂霉素C 卡介苗灌注法

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NP-20 Tetramethylpyrazine Nitrone Activates PGC-1α/Nrf2 Pathway to Prevent Neurodegeneration in Experimental Models of Parkinson’s Disease

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神经药理学报 2018年第4期8卷 117-118页

作者： ZHANG Zai-jun guo bao-jian ZHENG Cheng-you WANG Yu-qiang Institute of New Drug Research Jinan University College of PharmacyGuangzhou510632China

Objective:Disease-modifying therapies are not available for Parkinson’s disease(PD)and several trials targeting individual pathways implicated in PD pathogenesis have *** hypothesize that a potentially more powerful strategy is the molecule targeting multiple signaling cascades involved in *** is the conjugate of natural product tetramethylpyrazine(TMP)and the nitrone group of a neuroprotective agent *** neuroprotective effects of TBN have been verifi ed in various models of transient and permanent ischemic stroke,traumatic brain injury,as well as optic nerve injury through multifunctional *** objective of present study was to inverstigate the therapeutic effi cay and mechanisms of TBN on PD ***:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced mouse model,unilateral intrastriatal injection of 6-hydroxydopamine(6-OHDA)-induced rat model,and transgenic mice overexpression of human A53T mutantα-synuclein were used to evaluate TBN’s therapeutic efficacy and elucidate its mechanisms of ***:TBN takes the best of the Chinese medicine TMP,which has superior capability penetrating the blood-brain barrier(BBB),and the nitrone,which potently scavenging free *** a result,TBN effi ciently penetrates the BBB delivering free radical scavenging nitrone to the brain to afford protection to neuronal *** treatment partially restores motor impairment,increases content of DA and its metabolites,and reduces loss of hydroxylase positive(TH+)neurons in SNpc of 6-OHDA and MPTP-induced and transgenic rodent PD *** intriguing is that TBN signifi cantly preserves motor function and reduces serum level of alpha-synuclein by 85%in A53T alpha-synuclein transgenic mice after 8 weeks’*** also specifi cally suppresses MPTP intoxication-induced serum and mRNA levels of TNF-alpha and IL-6 in both wild-type and Rag1-/-immune deficient *** studies show that TBN targeting PGC-1alpha and Nrf2 activatio

关键词： Experimental PGC-1α treatment α-synuclein multiple expression strategy effects

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异种金属管复合塑性流动连接的流动分析

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锻压技术 2010年第4期35卷 112-115页

作者：刘飞飞包国建江西理工大学机电工程学院江西赣州341000

针对异种金属管材的连接需要,基于塑性流动原理,提出一种固相连接方法——复合塑性流动连接。结合流函数理论,对连接过程中金属塑性流动特性进行了分析,掌握了金属塑性流动的规律。并通过有限元软件对材料为铝合金LY12和紫铜T2的管材进... 详细信息

针对异种金属管材的连接需要,基于塑性流动原理,提出一种固相连接方法——复合塑性流动连接。结合流函数理论,对连接过程中金属塑性流动特性进行了分析,掌握了金属塑性流动的规律。并通过有限元软件对材料为铝合金LY12和紫铜T2的管材进行连接过程的仿真实验研究,模拟结果表明,金属的塑性流动情况与理论分析的结果一致。

关键词：异种金属管连接方法流函数塑性流动

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等径角挤压法制备超细晶工艺研究

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上海有色金属 2010年第2期31卷 70-73页

作者：包国建江西理工大学机电工程学院江西赣州341000

超细晶材料因具有较好的综合性能而倍受关注,等径角挤压法制备超细晶是目前研究的热点。介绍了等径角挤压法制备超细晶的原理,各参数对等径角挤压材料性能的影响,分析了等径角挤压法存在的问题以及等径角挤压材料的应用前景。

关键词：超细晶等径角挤压制备材料性能

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康北煤田大强井田煤层特征及煤层对比

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陕西煤炭 2015年第1期34卷 63-65页

作者：郭宝建东北煤田地质局一〇一勘探队辽宁调兵山112700

介绍了辽宁省康北煤田大强井田的地质构造,地层情况,分析了区内煤层的发育特征和煤层的沉积特征,以及煤层的对比方法,对比依据和对比的可靠程度。

关键词：大强井田煤层沉积特征煤层对比

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TBN improves motor function and prolongs survival in SOD1G93A and TDP-43M337V mouse model of amyotrophic lateral sclerosis

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中国药理学与毒理学杂志 2021年第9期35卷 647-648页

作者： HUANG Chun-hui LI Jun ZHANG Gui-liang LIN Ying-qi LI Cai-juan ZHENG Xiao SONG Xi-cheng HAN Bo-feng guo bao-jian TU Zhu-chi ZHANG Jun SUN Ye-wei WANG Yu-qiang YAN Sen ZHANG Zai-jun Institute of New Drug Research Jinan University College of PharmacyGuangzhou 510632China

OBJECTIVE Amyotrophic lateral sclerosis(ALS)is a fatal neurodegenerative dis⁃ease characterized by progressive loss of upper and lower motor neurons that results in skeletal muscle atrophy,weakness and ***⁃tive stress plays a key role in the pathogenesis of ALS,including familial forms of the disease arising from mutation of the gene coding for superoxide dismutase 1(SOD1).Moreover,although the pathogenesis of ALS is unclear,the abnormal accumulation of TAR DNA-binding pro⁃tein of 43 ku(TDP-43)is a pathological feature that exists in almost all *** far,there is no drug that can cure ALS/***⁃pyrazine nitrone(TBN)is a derivative of tetra⁃methylapyrazine,derived from the traditional Chinese medicine Ligusticum chuanxiong,which has been widely proven to have therapeutic effects on models of various neurodegenerative *** is currently under clinical investi⁃gation for several indications including a phaseⅡtrial of ***,we explored the therapeutic effect of TBN in the SOD1G93A and TDP-43M337V ALS mouse *** In the SOD1G93A transgenic mouse model,TBN was administered to mice by intraperitoneal or intragastric injection after the onset of motor *** the same time,we unilaterally and bilaterally injected the TDP-43M337V virus into the striatum of the WT mouse,and gave the TBN treatment after the mice developed a *** administering these two models for a period of time,we con⁃ducted behavioral tests,including rotarod test,balance beam test,climbing pole test,etc,to evaluate the efficacy of TBN on SOD1G93A and TDP-43M337V ***,we explored the possible mechanism of action of TBN in the treatment of ALS through Western blotting and immunohistochemistry/immunofluorescence staining *** In the SOD1G93A transgenic mouse model,TBN slowed the pro⁃gression of motor neuron disease as evidenced by improved motor performance,reduced spinal motor neuron loss and the associated glial respons

关键词： amyotrophic lateral sclerosis tetra⁃methylpyrazine nitrone superoxide dismutase 1

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Tetramethylpyrazine analogue T-006 promotes clearance of alpha-synuclein by enhancing proteasome activity in Parkinson disease models

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中国药理学与毒理学杂志 2019年第9期33卷 658-659页

作者： ZHOU He-feng SHAO Min guo bao-jian LI Chu-wen LU Yu-cong YANG Xuan-jun LI Sheng-nan LI Hai-tao ZHU Qi ZHONG Han-bing WANG Yu-qiang ZHANG Zai-jun LU Jia-hong LEE Ming-yuen Simon State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences University of MacaoMacao 999078China Department of Bioengineering Zhuhai Campus of Zunyi Medical CollegeZhuhai 519041China Institute of New Drug Research College of PharmacyJinan UniversityGuangzhou 510000China Department of Biology South University of Science and TechnologyShenzhen 518000China

OBJECTIVE To investigate the effects of T-006(tetramethylpyrazine derivative)in promotingα-Synuclein(α-Syn)degradation and evaluated the neuroprotective effects in cellular and animalα-Syn model of Parkinson disease(PD).METHODS The inducible PC12 cells overexpressingα-syn and the homozygous transgenic(Tg)mice expressing A53T humanα-syn were used to evaluate the neuroprotective effects of *** cellular study,MTT,Western blotting,proteasomal activity assay and qRT-PCR were applied to analyze the pharmacological effects and underlying mecha⁃*** gene knock-down and overexpression approaches were used to dissect the molecular signaling *** animal study,ten-month-old homozygousα-Syn Tg mice were treated with T-006(3 mg·kg-1)daily by gavage for four *** Western blotting,immunohistochemistry and behavioral tests were applied to determine the neuropatho⁃logical *** T-006 promoted the degradation of WT and mutantα-Syn in PC12α-Syn inducible cells via an ubiquitin-proteasome system(UPS)dependent and autophagy-lysosome pathway independent *** mecha⁃nism of action involved the upregulation of 20S proteasome subunit LMP7 expression,which leads to activation of the chymotrypsin-like proteasomal activity for protein ***,we demonstrated that T-006 activated PKA/Akt/mTOR pathway upstream for LMP 7 up-regulation and UPS ***,we illustrated that T-006 promoted both Triton-soluble and-insoluble forms ofα-syn and protected againstα-Syn-induced neurotoxicity in A53Tα-Syn Tg *** T-006 is a potent UPS activator which promotes the degradation of pathogenic proteinα-Syn in cellular and animal PD *** study thus high-lights the therapeutic potential of small molecular UPS activator like T-006 in the treatment of PD and related conditions.

关键词： α-synuclein degradation LMP7 proteasome activity Parkinson disease

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