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Genes & Diseases 2024年第3期11卷 206-219页

作者： Shuyu Li Zichen Xu Yi wang Lizhao Chen Xiangxiu wang Yanghao Zhou Daoxi Lei Guangchao Zang guixue wang Key Laboratory of Biorheological Science and Technology of Ministry of Education National and Local Joint Engineering Laboratory for Vascular ImplantsBioengineering College of Chongqing UniversityChongqing 400030China College of Basic Medical Sciences Chongqing Medical UniversityChongqing 400016China Department of Neurosurgery Daping HospitalArmy Medical Center of PLAArmy Medical UniversityChongqing 400042China

Atherosclerotic cardiovascular disease and its complications are a high-incidence disease worldwide.Numerous studies have shown that blood flow shear has a huge impact on the function of vascular endothelial cells,and it plays an important role in gene regulation of pro-inflammatory,pro-thrombotic,pro-oxidative stress,and cell permeability.Many impor-tant endothelial cell mechanosensitive genes have been discovered,including KLK10,CCN gene family,NRP2,YAP,TAZ,HIF-1α,NF-kB,FOS,JUN,TFEB,KLF2/KLF4,NRF2,and ID1.Some of them have been intensively studied,whereas the relevant regulatory mechanism of other genes remains unclear.Focusing on these mechanosensitive genes will provide new strategies for therapeutic intervention in atherosclerotic vascular disease.Thus,this article reviews the mechanosensitive genes affecting vascular endothelial cells,including classical pathways and some newly screened genes,and summarizes the latest research progress on their roles in the pathogenesis of atherosclerosis to reveal effective therapeutic targets of drugs and provide new insights foranti-atherosclerosis.

关键词： Atherosclerosis Blood flow Endothelial cell Mechanosensitive gene Therapeutic target

Effects of COVID-19 pandemic on human fertility:A scientometric and visualized evaluation

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Genes & Diseases 2024年第4期11卷 113-115页

作者： Xiangxiu wang Yifan Rao Lili Tan Ziqiu Hu Lin Wen Weixi Qin Bingyi Li Keqiao He Tieying Yin Juhui Qiu Yuming wang Yaokai Chen Xiancai Rao guixue wang Key Laboratory of Biorheological and Technology of the Ministry of Education State and Local Joint Engineering LaboratoryforVascular ImplantsModern LifeScience Experiment Teaching Center at Bioengineering College of Chongqing UniversityChongqing 400030China Department of Microbiology College of Basic Medical SciencesKey Laboratory of Microbial Engineering Under the Educational Committee in ChongqingArmy Medical University(Third Military Medical University)Chongqing 400038China Jinfeng Laboratory Chongqing 401329China Division of Infectious Diseases Chongqing Public Health Medical CenterChongqing 400036China

CovID-19,also known as coronavirus disease 2019,is a novel coronavirus disease with high infectivity,strong heterogeneity,and long incubation period(generally 3-14 days).Its main symptoms and signs include fever,dry cough,nasal congestion,fatigue,disorientation,lymphopenia,and dyspnea.The short-term and long-term impacts of covID-19 on human health,particularly its effects on human reproduction and offspring development,continue to receive significant concerns,as they may lead to potential sequelae for several decades or even centuries.

关键词： nasal fertility fever

A Novel Poly(3-hexylthiophene) Engineered Interface for Electrochemical Monitoring of Ascorbic Acid During the Occurrence of Glutamate-Induced Brain Cytotoxic Edemas

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Research 2024年第1期2023卷 301-313页

作者： Zexuan Meng Yuchan Zhang Lu Yang Shuang Zhao Qiang Zhou Jiajia Chen Jiuxi Sui Jian wang Lizhong Guo Luyue Chang Jialing He guixue wang Guangchao Zang Institute of Life Science and Laboratory of Tissue and Cell BiologyLab Teaching and Management CenterChongqing Medical UniversityChongqing 400016China Key Laboratory for Biorheological Science and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular ImplantsBioengineering College of Chongqing UniversityChongqing 400030China Jinfeng Laboratory Chongqing 401329China Department of Pathophysiology Chongqing Medical UniversityChongqingChina

Although neuroelectrochemical sensing technology offers unique benefits for neuroscience research,its application is limited by substantial interference in complex brain environments while ensuring biosafety requirements.In this study,we introduced poly(3-hexylthiophene)(P3HT)and nitrogen-doped multiwalled carbon nanotubes(N-MWCNTs)to construct a composite membrane-modified carbon fiber microelectrode(CFME/P3HT-N-MWCNTs)for ascorbic acid(AA)detection.The microelectrode presented good linearity,selectivity,stability,antifouling,and biocompatibility and exhibited great performance for application in neuroelectrochemical sensing.Subsequently,we applied CFME/P3HT-N-MWCNTs to monitor AA release from in vitro nerve cells,ex vivo brain slices,and in vivo living rat brains and determined that glutamate can induce cell edema and AA release.We also found that glutamate activated the N-methyl-d-aspartic acid receptor,which enhanced Na^(+) and Cl^(−) inflow to induce osmotic stress,resulting in cytotoxic edema and ultimately AA release.This study is the first to observe the process of glutamate-induced brain cytotoxic edema with AA release and to reveal the mechanism.Our work can benefit the application of P3HT in in vivo implant microelectrode construction to monitor neurochemicals,understand the molecular basis of nervous system diseases,and discover certain biomarkers of brain diseases.

关键词： release microelectrode Engine

The Janus-faced role of Piezo1 in cardiovascular health under mechanical stimulation

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Genes & Diseases 2023年第5期10卷 1956-1968页

作者： Junyang Huang Kun Zhang Ruolin Du Wanling Liu Haijun Zhang Tian Tian Yazhou wang guixue wang Tieying Yin Key Laboratory for Biorheological Science and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular ImplantsBioengineering College of Chongqing UniversityChongqing 400030China Department of Vascular&Intervention Tenth Peoples'Hospital of Tongji UniversityShanghai 200072China School of Medicine Chongqing UniversityChongqing 400030China

In recent years,cardiovascular health problems are becoming more and more serious.At the same time,mechanical stimulation closely relates to cardiovascular health.In this context,Piezo1,which is very sensitive to mechanical stimulation,has attracted our attention.Here,we review the critical significance of Piezo1 in mechanical stimulation of endothelial cells,NO production,lipid metabolism,DNA damage protection,the development of new blood vessels and maturation,narrowing of blood vessels,blood pressure regulation,vascular permeability,insulin sensitivity,and maintenance of red blood cell function.Besides,Piezo1 may participate in the occurrence and development of atherosclerosis,diabetes,hypertension,and other cardiovascular diseases.It is worth noting that Piezo1 has dual effects on maintaining cardiovascular health.On the one hand,the function of Piezo1 is necessary to maintain cardiovascular health;on the other hand,under some extreme mechanical stimulation,the overexpression of Piezo1 may bring adverse factors such as inflammation.Therefore,this review discusses the Janus-faced role of Piezo1 in maintaining cardiovascular health and puts forward new ideas to provide references for gene therapy or nanoagents targeting Piezo1.

关键词： Atherosclerosis Cardiovascular disease Endothelial cells(ECs) Mechanical stimulation Piezo1

Mechano-sensitive TET1s inhibits endothelial athero-susceptible phenotype through regulating YAP phosphorylation

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Genes & Diseases 2023年第4期10卷 1183-1186页

作者： Lu Huang Kai Qu Caihong wang Daoxi Lei Wenhua Yan Tianhan Li Zhengjun Hou Juhui Qiu guixue wang Key Laboratory for Biorheological Science and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular ImplantsBioengineering College of Chongqing UniversityChongqing 400030China School of Advanced Manufacturing Engineering Chongqing University of Posts and TelecommunicationsChongqing 400065China Department of Ophthalmology Chongqing Hospital of Traditional Chinese MedicineChongqing 400021China The Second Affiliated Hospital of Chongqing Medical University Chongqing 400010China

Atherosclerosis is a chronic progressive disease and one of the major causes of cardio-cerebral vascular diseases.Accumulating evidence indicates that endothelial dysfunc-tion is the initiating step in atherosclerosis.The pattern of local blood flow becomes disturbed(low and oscillatory shear stress[OSS])in the curved or branched segments of the arterial tree,causing endothelial cells(ECs)to exhibit athero-susceptible phenotypes,such as hyperproliferation and inflammation.

关键词： cerebral arterial atherosclerosis

Structural and temporal dynamics analysis on drug-eluting stents: History, research hotspots and emerging trends

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Bioactive Materials 2023年第5期23卷 170-186页

作者： Lili Tan Xiangxiu wang Kungshan Yuan Tieying Yin Ruolin Du Li Shen Zhirong Zhu Suhua Yu Haijun Zhang guixue wang Key Laboratory of Biorheological and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular ImplantsModern Life Science Experiment Teaching Center at Bioengineering College of Chongqing UniversityChongqing400030China National United Engineering Laboratory for Biomedical Material Modification Shandong Rientech Medical Tech Co.LtdDezhouShandong251100China Department of Cardiology Zhongshan HospitalFudan UniversityShanghai Institute of Cardiovascular DiseasesShanghaiNational Clinical Research Center for Interventional MedicineShanghai200032China Shanghai Key Lab of Chemical Assessment and Sustainability School of Chemical Science and EngineeringTongji UniversityShanghai200092China Blue Sail Pioneer Lab JW Medical SystemsJiwei Shandong264200China JinFeng Laboratory Chongqing401329China

Purpose:This review aims to explore the history,research hotspots,and emerging trends of drug-eluting stents(DES)in the last two decades from the perspective of structural and temporal dynamics.Methods:Publications on DES were retrieved from WoSCC.The bibliometric tools including CiteSpace and HistCite were used to identify the historical features,the evolution of active topics,and emerging trends on the DES field.Results:In the last 20 years,the field of DES is still in the hot phase and there is a wide range of extensive scientific collaborations.In addition,active topics emerge in different periods,as evidenced by a total of 41 disciplines,511 keywords,and 1377 papers with citation bursts.Keyword clustering anchored five emerging research subfields,namely#0 dual antiplatelet therapy,#3 drug-coated balloon,#4 bifurcation,5#rotational atherectomy,and 6#quantitative flow ratio.The keyword alluvial map shows that the most persistent research concepts in this field are thrombosis,restenosis,etc.,and the emerging keywords are paclitaxel eluting balloon,coated balloon,drug-eluting balloon,etc.There are 7 recent research subfields anchored by reference clustering,namely#2 dual antiplatelet therapy,#4 drug-coated balloon,#5 peripheral artery disease,#8 fractional flow reserve,#10 bioresorbable vascular scaffold,#13 intravascular ultrasound,#14 biodegradable polymer.Conclusion:The findings based on the bibliometric studies provide the current status and trends in DES research and may help researchers to identify hot topics and explore new research directions in this field.

关键词： Drug-eluting stents Dual antiplatelet therapy Drug-coated balloon Bioresorbable scaffold Bibliometric

High performance of multi-layered alternating Ni-Fe-P and Co-P films for hydrogen evolution

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Green Energy & Environment 2022年第1期7卷 75-85页

作者： Zunhang Lv Kaihang wang Yingying Si Zihan Li Tianpeng Yu Xin Liu guixue wang Guangwen Xie Luhua Jiang College of Materials Science and Engineering Qingdao University of Science and TechnologyQingdao266042China

Judiciously engineering the electrocatalysts is attractive and challenging to exploit materials with high electrocatalytic performance for hydrogen evolution reaction.Herein,we successfully perform the interface engineering by alternately depositing Co±P and Ni±Fe±P films on nickel foam,via facile electroless plating and de-alloying process.This work shows that there is a significant effect of de-alloying process on alloy growth.The electronic structure of layered alloys is improved by interface engineering.The multilayer strategy significantly promotes the charge transfer.Importantly,the Co±P/Ni±Fe±P/NF electrode fabricated by interface engineering exhibits excellent electrocatalytic hydrogen evolution activity with an overpotential of 43.4 mV at 10 mA cm^(-2)and long-term durability for 72 h in alkaline medium(1 mol L^(-1)KOH).The innovative strategy of this work may aid further development of commercial electrocatalysts.

关键词： Interface engineering Electroless plating Amorphous materials Hydrogen evolution reaction Electrocatalysts

The recent advances and future perspectives of genetic compensation studies in the zebrafish model

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Genes & Diseases 2023年第2期10卷 468-479页

作者： Muhammad Abdul Rouf Lin Wen Yoga Mahendra Jinxuan wang Kun Zhang Shuang Liang Yuming wang Zhenggong Li Yeqi wang guixue wang Key Laboratory for Biorheological Science and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular Implants Bioengineering College of Chongqing University Chongqing 400030China Center of Cardiology Chongqing General HospitalUniversity of Chinese Academy of SciencesChongqing 400013China

Genetic compensation is a remarkable biological concept to explain the genetic robustness in an organism to maintain its fitness and viability if there is a disruption occurred in the genetic variation by mutation.However,the underlying mechanism in genetic compensation remain unsolvable.The initial concept of genetic compensation has been studied in model organisms when there was a discrepancy between knockout-mediated and knockdown-mediated phenotypes.In the zebrafish model,several studies have reported that zebrafish mutants did not exhibit severe phenotype as shown in zebrafish morphants for the same genes.This phenomenon in zebrafish mutants but not morphants is due to the response of genetic compensation.In 2019,two amazing works partially uncovered genetic compensation could be triggered by the upregulation of compensating genes through regulating NMD and/or PTC-bearing mRNA in collaboration with epigenetic machinery in mutant zebrafish.In this review,we would like to update the recent advances and future perspectives of genetic compensation studies,which including the hypothesis of time-dependent involvement and addressing the discrepancy between knockout-mediated and knockdown-mediated to study gene function in the zebrafish model.At last,the study of genetic compensation could be a potential therapeutic strategy to treat human genetic disorder related diseases.

关键词： Epigenetic machinery Genetic compensation Genetic robustness Knockdown-mediated Knockout-mediated NMD PTC Zebrafish

Particle Size-Controlled Oxygen Reduction and Evolution Reaction Nanocatalysts Regulate Ru(bpy)_(3)^(2+)’s Dual-potential Electrochemiluminescence for Sandwich Immunoassay

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Research 2023年第4期2023卷 701-715页

作者： Shijun wang Shu Zhu Ziqi Kang Xiangxiu wang Zixin Deng Kun Hu Jianjun Hu Xiancheng Liu guixue wang Guangchao Zang Yuchan Zhang Institute of Life Science and Laboratory of Tissue and Cell Biology Lab Teaching&Management CenterChongqing Medical UniversityChongqing 400016China Key Laboratory for Biorheological Science and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular ImplantsBioengineering College of Chongqing UniversityChongqing 400030China Jinfeng Laboratory Chongqing 401329China Department of Pathophysiology Chongqing Medical UniversityChongqing 400016China Department of Pathology Guizhou Provincial People’s HospitalGuiyangGuizhou 550002China

Multiple signal strategies remarkably improve the accuracy and efficiency of electrochemiluminescence(ECL)immunoassays,but the lack of potential-resolved luminophore pairs and chemical cross talk hinders their development.In this study,we synthesized a series of gold nanoparticles(AuNPs)/reduced graphene oxide(Au/rGO)composites as adjustable oxygen reduction reaction and oxygen evolution reaction catalysts to promote and modulate tris(2,2′-bipyridine)ruthenium(II)(Ru(bpy)_(3)^(2+))’s multisignal luminescence.With the increase in the diameter of AuNPs(3 to 30 nm),their ability to promote Ru(bpy)_(3)^(2+)’s anodic ECL was first impaired and then strengthened,and cathodic ECL was first enhanced and then weakened.Au/rGOs with medium-small and medium-large AuNP diameters remarkably increased Ru(bpy)_(3)^(2+)’s cathodic and anodic luminescence,respectively.Notably,the stimulation effects of Au/rGOs were superior to those of most existing Ru(bpy)_(3)^(2+)co-reactants.Moreover,we proposed a novel ratiometric immunosensor construction strategy using Ru(bpy)_(3)^(2+)’s luminescence promoter rather than luminophores as tags of antibodies to achieve signal resolution.This method avoids signal cross talk between luminophores and their respective co-reactants,which achieved a good linear range of 10−7 to 10−1 ng/ml and a limit of detection of 0.33 fg/ml for detecting carcinoembryonic antigen.This study addresses the previous scarcity of the macromolecular co-reactants of Ru(bpy)_(3)^(2+),broadening its application in biomaterial detection.Furthermore,the systematic clarification of the detailed mechanisms for converting the potential-resolved luminescence of Ru(bpy)_(3)^(2+)could facilitate an in-depth understanding of the ECL process and should inspire new designs of Ru(bpy)_(3)^(2+)luminescence enhancers or applications of Au/rGOs to other luminophores.This work removes some impediments to the development of multisignal ECL biodetection systems and provides vitality into their widespread applications.

关键词： luminescence potential reactant

Cathepsin K contributed to disturbed flow-induced atherosclerosis is dependent on integrin-actin cytoskeleton–NF–κB pathway

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Genes & Diseases 2023年第2期10卷 583-595页

作者： Fei Fang Tang Feng Jianwei Li Huaiyi Zhang Qin wang Yidan Chen guixue wang Yang Shen Xiaoheng Liu Institute of Biomedical Engineering West China School of Basic Medical Sciences & Forensic MedicineSichuan UniversityChengduSichuan 610041China Department of Endocrinology and Metabolism West China HospitalSichuan UniversityChengduSichuan 610041China Key Laboratory for Biorheological Science and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular ImplantsCollege of BioengineeringChongqing UniversityChongqing 400030China

Atherosclerosis is a chronic inflammatory disease, occurring preferentially in bifurcation, branching, and bending of blood vessels exposed to disturbed flow. Disturbed flow in atheroprone areas activates elevated proteases, degrading elastin lamellae and collagenous matrix, resulting in endothelial dysfunction and vascular remodeling. As a mediator for extracellular matrix protein degradation, cathepsin K (CTSK) was directly regulated by hemodynamics and contributed to atherosclerosis. The mechanism of CTSK responding to disturbed flow and contributing to disturbed flow-induced atherosclerosis is unclear. In this study, the partial carotid ligation model of mice and in vitro disturbed shear stress model were constructed to explore the contribution and potential mechanism of CTSK in atherosclerosis. Our results indicated that CTSK elevated in the disturbed flow area in vivo and in vitro along with endothelial inflammation and atherogenesis. Additionally, the expression of integrin αvβ3 was upregulated in these atheroprone areas. We found that inhibition of the integrin αvβ3-cytoskeleton pathway could significantly block the activation of NF-κB and the expression of CTSK. Collectively, our findings unraveled that disturbed flow induces increased CTSK expression, and contributes to endothelial inflammation and vascular remodeling, leading to atherogenesis eventually. This study is helpful to provide new enlightenment for the therapy of atherosclerosis.

关键词： Atherosclerosis Cathepsin K Disturbed flow Integrin NF-κB